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The map label for this gene is def [H]

Identifier: 78224529

GI number: 78224529

Start: 3753435

End: 3753938

Strand: Direct

Name: def [H]

Synonym: Gmet_3338

Alternate gene names: 78224529

Gene position: 3753435-3753938 (Clockwise)

Preceding gene: 78224528

Following gene: 78224530

Centisome position: 93.9

GC content: 56.94

Gene sequence:

>504_bases
ATGGTGCGTACAATTCTTACTTATCCCAACCCGATCCTCAAAAAGAAGGCGGTGCCGGTTGCGGTCATCAACGACGCGAC
CCGTGAACTGGTCCGCGACATGGCCGAAACCATGTATGACGCCCAGGGGGTCGGCCTGGCCGCCCCGCAGATCGGCGTAA
GCCAGCGGGTCATCGTCATTGATGTCTCTCAGCGCGAAGAAAGGCCGGAGCTGATTGTCTGCATCAACCCGGTGTTCGTC
CATACCGAAGGGGAATCATACGAGGAAGAGGGTTGCCTCTCTGTCCCCAAGTACTCCGCCAATGTGCACCGGCACGCCAA
GGTTGTCGTTAAGGCGCTCAATCTCGATGGAGAGGAAGTGACCTATAGGGCGGAAGGGCTTCTGGCCATCGCTTTCCAGC
ACGAGATAGACCACCTGGAAGGGATGCTCTTCGTGGATCACCTTTCTCCCCTGAAGCGGGAGATGTTCAAGAAAAAATAC
CGCCGCATGGTTGAGGAAGGGTAG

Upstream 100 bases:

>100_bases
GGATCTCATGTGACTTCGCCGTCCCGTCAGGAAGGGTTTGATTTTTTCGCCCTATCTCTCTATATTCATACGAATAACTA
TCGTTGAAGGTGCATGAACC

Downstream 100 bases:

>100_bases
GATTACTGCATGGCCGGACTCAGCATCATCTTCATGGGAACCCCTGATTTCGCCTGTCCCACGCTCACGAGACTCATCGA
GCGAGGCGAGGATGTAATTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MVRTILTYPNPILKKKAVPVAVINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVIDVSQREERPELIVCINPVFV
HTEGESYEEEGCLSVPKYSANVHRHAKVVVKALNLDGEEVTYRAEGLLAIAFQHEIDHLEGMLFVDHLSPLKREMFKKKY
RRMVEEG

Sequences:

>Translated_167_residues
MVRTILTYPNPILKKKAVPVAVINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVIDVSQREERPELIVCINPVFV
HTEGESYEEEGCLSVPKYSANVHRHAKVVVKALNLDGEEVTYRAEGLLAIAFQHEIDHLEGMLFVDHLSPLKREMFKKKY
RRMVEEG
>Mature_167_residues
MVRTILTYPNPILKKKAVPVAVINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVIDVSQREERPELIVCINPVFV
HTEGESYEEEGCLSVPKYSANVHRHAKVVVKALNLDGEEVTYRAEGLLAIAFQHEIDHLEGMLFVDHLSPLKREMFKKKY
RRMVEEG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=32.484076433121, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=47.5308641975309, Blast_Score=145, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=35.6687898089172, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.6455696202532, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18884; Mature: 18884

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRTILTYPNPILKKKAVPVAVINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVI
CCEEEEECCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DVSQREERPELIVCINPVFVHTEGESYEEEGCLSVPKYSANVHRHAKVVVKALNLDGEEV
ECCCCCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCE
TYRAEGLLAIAFQHEIDHLEGMLFVDHLSPLKREMFKKKYRRMVEEG
EEECCCEEEEEEEHHHHHHCCCCEEHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVRTILTYPNPILKKKAVPVAVINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVI
CCEEEEECCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DVSQREERPELIVCINPVFVHTEGESYEEEGCLSVPKYSANVHRHAKVVVKALNLDGEEV
ECCCCCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCE
TYRAEGLLAIAFQHEIDHLEGMLFVDHLSPLKREMFKKKYRRMVEEG
EEECCCEEEEEEEHHHHHHCCCCEEHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA