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The map label for this gene is thlA [H]

Identifier: 78224493

GI number: 78224493

Start: 3714912

End: 3716087

Strand: Direct

Name: thlA [H]

Synonym: Gmet_3302

Alternate gene names: 78224493

Gene position: 3714912-3716087 (Clockwise)

Preceding gene: 78224492

Following gene: 78224499

Centisome position: 92.93

GC content: 64.97

Gene sequence:

>1176_bases
ATGAATGAAATATTTGTGGTTGATGCTCTGAGAACACCGTTTGGTTCATTTTCCGGCATGCTGGCCGACGTGGATGCCCC
AAAGCTGGCCGCCACCGTCATGAAAGAGCTTCTGGCAAAGGCCGGCCTCGCCCCCGACACGGTGAATGAAGTCATTGTGG
GGCAGGTCCTCTCCGGCGGCTGTGGCCAAGCCCCGGCCCGCCAGGCCATGCGCGGGGCGGGAATCCCCGACACGGCCCAT
GCCCTGACCGTCAACAAGGTCTGCGGAAGCGGCCTGAAGGCAATCATGCTCGGCGCCGACAGCATTCGTCTCGGTGAGTC
CCAAATTGTCATCGCCGGCGGCATGGAGAACATGTCCCTGGCGCCCTTTTTCCTCAAGAAGGCCCGCGCCGGCTACCGCA
TGGGGCACAGTGAACTGCTGGACCTGCTGATCTACGACGGTCTCCAGGATCCCTATACGGGCCGCCACATGGGAGAGATC
GGTGAGGCGAGCGCGGAGCGGGGCAACCTCTCTCGCGAGGAGCAGGACGCGTTCGCCCGGCGCTCCTACGAACTGGCCCA
GGCGGCGGTGACCGGCCGCATCTTTGCCGACGAGATCGTGCCGGTGGTAAAGAAGGGGAAAAAGGGTGACGAAACCATTG
GCACGGACGAAGAGCCCTTCAAGGGTGATCCGAGCAAGCTGCCATCTCTGCGGGCCGCCTTCAAGAAGGACGGCACCATC
ACCGCCGGCAACGCCTCCACCATCAACGACGGCGCGGCCCTGGCCCTACTGACCGATGCCGACGGCCTAAAGAAGTACAA
CCTCACCCCCAAGGCCCGGATCGTTGCCTCGGCCACGGAAAGCCGTCATCCCGACAACTTCCCCGAGGCCCCCATCGGCG
CCATCGAGAAGGCCTGCGCCAAGGCCGGCCTGACGGTGTCCGACATCGACCTCTTCGAGATCAACGAGGCCTTTGCCTCG
GTGGCCCTCCTGGCCGTCAAGGGTCTCGGCCTCCCCTTGGACAAGGTGAACGTCAACGGCGGCGCCTGCGCCATCGGCCA
CCCCATCGGCGCCAGCGGCGGCCGGCTTGCAGCCACGGTCATCCGGGAACTCCATCGGCGGCAGAAACGCTACGGCCTCG
CCACCCTCTGCATCGGCGGCGGCGAGGCGGTGGCAGTCATCTTCGAGCGGGTTTGA

Upstream 100 bases:

>100_bases
GCCGATGTCATGCAGTTCGTGCCGGCCCTGACAGCACGGGTCATGCGGTGACAAACGGCATATCTTCTACAAAAGTGGCA
CAGACTATCGGGAGGCTTCG

Downstream 100 bases:

>100_bases
TATTAAAAATCCACCACGGAGACAGGGTGGCACGGAAAAAGGCAAAGAAATGGGGTTCGTTTCCCAGGTTATCTCAACGC
CTCCGTCTCTCAGTGGTTGC

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNEIFVVDALRTPFGSFSGMLADVDAPKLAATVMKELLAKAGLAPDTVNEVIVGQVLSGGCGQAPARQAMRGAGIPDTAH
ALTVNKVCGSGLKAIMLGADSIRLGESQIVIAGGMENMSLAPFFLKKARAGYRMGHSELLDLLIYDGLQDPYTGRHMGEI
GEASAERGNLSREEQDAFARRSYELAQAAVTGRIFADEIVPVVKKGKKGDETIGTDEEPFKGDPSKLPSLRAAFKKDGTI
TAGNASTINDGAALALLTDADGLKKYNLTPKARIVASATESRHPDNFPEAPIGAIEKACAKAGLTVSDIDLFEINEAFAS
VALLAVKGLGLPLDKVNVNGGACAIGHPIGASGGRLAATVIRELHRRQKRYGLATLCIGGGEAVAVIFERV

Sequences:

>Translated_391_residues
MNEIFVVDALRTPFGSFSGMLADVDAPKLAATVMKELLAKAGLAPDTVNEVIVGQVLSGGCGQAPARQAMRGAGIPDTAH
ALTVNKVCGSGLKAIMLGADSIRLGESQIVIAGGMENMSLAPFFLKKARAGYRMGHSELLDLLIYDGLQDPYTGRHMGEI
GEASAERGNLSREEQDAFARRSYELAQAAVTGRIFADEIVPVVKKGKKGDETIGTDEEPFKGDPSKLPSLRAAFKKDGTI
TAGNASTINDGAALALLTDADGLKKYNLTPKARIVASATESRHPDNFPEAPIGAIEKACAKAGLTVSDIDLFEINEAFAS
VALLAVKGLGLPLDKVNVNGGACAIGHPIGASGGRLAATVIRELHRRQKRYGLATLCIGGGEAVAVIFERV
>Mature_391_residues
MNEIFVVDALRTPFGSFSGMLADVDAPKLAATVMKELLAKAGLAPDTVNEVIVGQVLSGGCGQAPARQAMRGAGIPDTAH
ALTVNKVCGSGLKAIMLGADSIRLGESQIVIAGGMENMSLAPFFLKKARAGYRMGHSELLDLLIYDGLQDPYTGRHMGEI
GEASAERGNLSREEQDAFARRSYELAQAAVTGRIFADEIVPVVKKGKKGDETIGTDEEPFKGDPSKLPSLRAAFKKDGTI
TAGNASTINDGAALALLTDADGLKKYNLTPKARIVASATESRHPDNFPEAPIGAIEKACAKAGLTVSDIDLFEINEAFAS
VALLAVKGLGLPLDKVNVNGGACAIGHPIGASGGRLAATVIRELHRRQKRYGLATLCIGGGEAVAVIFERV

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=43.2569974554707, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=45.4081632653061, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=40.2061855670103, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=39.2405063291139, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=30.9133489461358, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194353979, Length=389, Percent_Identity=32.3907455012853, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=44.6428571428571, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=46.3104325699746, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=41.5422885572139, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=36.6748166259169, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1788683, Length=415, Percent_Identity=28.6746987951807, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=41.3881748071979, Blast_Score=310, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI25147385, Length=387, Percent_Identity=41.6020671834625, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17535921, Length=391, Percent_Identity=38.8746803069054, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=32.8282828282828, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=33.2547169811321, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6325229, Length=394, Percent_Identity=43.1472081218274, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6322031, Length=400, Percent_Identity=35.5, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.1530612244898, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=43.9897698209719, Blast_Score=310, Evalue=8e-85,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=38.3248730964467, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.0754716981132, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40850; Mature: 40850

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIFVVDALRTPFGSFSGMLADVDAPKLAATVMKELLAKAGLAPDTVNEVIVGQVLSGG
CCCEEEEECHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
CGQAPARQAMRGAGIPDTAHALTVNKVCGSGLKAIMLGADSIRLGESQIVIAGGMENMSL
CCCCHHHHHHHCCCCCCCHHHEEHHHHHCCCCEEEEECCCCEEECCCEEEEECCCCCCCH
APFFLKKARAGYRMGHSELLDLLIYDGLQDPYTGRHMGEIGEASAERGNLSREEQDAFAR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RSYELAQAAVTGRIFADEIVPVVKKGKKGDETIGTDEEPFKGDPSKLPSLRAAFKKDGTI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHCCCCE
TAGNASTINDGAALALLTDADGLKKYNLTPKARIVASATESRHPDNFPEAPIGAIEKACA
ECCCCCCCCCCEEEEEEECCCCCEECCCCCCCEEEEECHHCCCCCCCCCCCHHHHHHHHH
KAGLTVSDIDLFEINEAFASVALLAVKGLGLPLDKVNVNGGACAIGHPIGASGGRLAATV
HCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCEEEECCCCCCCCCHHHHHH
IRELHRRQKRYGLATLCIGGGEAVAVIFERV
HHHHHHHHHHCCEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MNEIFVVDALRTPFGSFSGMLADVDAPKLAATVMKELLAKAGLAPDTVNEVIVGQVLSGG
CCCEEEEECHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
CGQAPARQAMRGAGIPDTAHALTVNKVCGSGLKAIMLGADSIRLGESQIVIAGGMENMSL
CCCCHHHHHHHCCCCCCCHHHEEHHHHHCCCCEEEEECCCCEEECCCEEEEECCCCCCCH
APFFLKKARAGYRMGHSELLDLLIYDGLQDPYTGRHMGEIGEASAERGNLSREEQDAFAR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RSYELAQAAVTGRIFADEIVPVVKKGKKGDETIGTDEEPFKGDPSKLPSLRAAFKKDGTI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHCCCCE
TAGNASTINDGAALALLTDADGLKKYNLTPKARIVASATESRHPDNFPEAPIGAIEKACA
ECCCCCCCCCCEEEEEEECCCCCEECCCCCCCEEEEECHHCCCCCCCCCCCHHHHHHHHH
KAGLTVSDIDLFEINEAFASVALLAVKGLGLPLDKVNVNGGACAIGHPIGASGGRLAATV
HCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCEEEECCCCCCCCCHHHHHH
IRELHRRQKRYGLATLCIGGGEAVAVIFERV
HHHHHHHHHHCCEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]