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The map label for this gene is stc [H]

Identifier: 78224499

GI number: 78224499

Start: 3722946

End: 3724592

Strand: Direct

Name: stc [H]

Synonym: Gmet_3308

Alternate gene names: 78224499

Gene position: 3722946-3724592 (Clockwise)

Preceding gene: 78224493

Following gene: 78224501

Centisome position: 93.13

GC content: 60.96

Gene sequence:

>1647_bases
ATGGATACACATACCCCCGCTTCGCTGTTCCTTAAAGCAGACTATTGCAGATGCGGTATTGTCCTCCTCGACGAATCAGG
CCGCGTTACCGCCCTGAACCGGCAGTTTTTCGACACGACAGGCCTGGAGTTCAGAGAGGGTGATCTCCCTCCCGACGAGT
TGCGGGGGCTGAAGAAAGGAGAGTGGATTTTCTGGGGGAGGAAGTGGTTCGTGCAAGCGGAGGGGCGGCACGGGGTGGAG
CTGCTGGCAATCCGGCGCGTTCGGGCTGCGGACCATATACTGGGGCCGTACGGAGAGAACTGCCTGAATGAAGAGATGGT
GAAAATGGTCCTCGACAACCCCTACGAGGGACTGACGGTCGTCGACGCTACGGGGAAGGTCACCCTGCTCAGCCCGTCCA
ACGAGCAGTGGCTGGGGCTGGAAGTCGGAGGGGGACGGGGCCTCGACCTGTCGGCCATTGCGCCGGCCAGCCATTTGTCG
GAGGTGGCCCGCACCGGCGTGGCCGAACACGCCCAGGTTGTCGACCTGCACGGCAAGACCAAGGTCACGGTGAACCTTCC
CATCCGGCGGGACAACAAGGTGATGGGCGCTTTCGGGAGAATCCTCTTCAAGAGCACGGAACAAGTGGAGAAGCTGGTGA
CCAAGGTGCGGACCATGGAACTCCAGGTGGAGCGTTTCGAGACCCTGCTGGATGAAATGCGGGGGAAACGCTACTCCTTC
CGTAACATCCTCACCAAAAACAAAGAAATGCTGATGCTCATCGAACAGGCACGACGCGTGGCCGACTCCGGCGCGACCGT
GCTCATCCTGGGAGAAAGCGGGACTGGCAAGGAGTTGTTCGCCCAGGCGCTCCACGAAGCTTCGAGGAGGAACAAGGGGC
CTTTCATTGCAGTAAACTGCGGCGCAATCCCCCGCGACCTGATCGAATCGGAACTGTTCGGCTACGAAGAAGGGGCCTTC
AGCGGCGCCAGGAAAAAGGGGAAGCCCGGCAAGTTCGAACTGGCGTGCGGCGGAACGCTCTTTCTGGATGAGATCGGTGA
ACTCCCCCTCGAAAGTCAGGCAAAGCTGCTGCGGGTGCTTGAGGAGCGTAAGATAGACCGCCTGGGAGGCACGGCGCCGC
TCCCCGTCGATTTTCGCCTGGTGGCCGCCACGAACCGCGATCTCATGACGAACGTCAACAAAGGGAGATTCCGCGCCGAT
CTCTATTACCGCATCAACGAGTTCCCCCTCGAGATTCCCCCCCTCAGGTCCAGACCGGAAGACATCCCCCTGCTGGCGAA
GCATTTTCTGACGGAAGTGTCACGCCGGGAAGAACTGCCGATGCTCAAGATTTCCGCAGGAGCCACGGAGGCGTTGATAT
CGCACGACTGGCCCGGCAATGTACGGGAACTGCGCGGGGTCATGCGTCAGATGGCCTGGAAATCCCAGGGATTAACCATA
GAGCCCCACCACCTGCCGCCGGCGCTCAACAAGGGACAGACCGTCGGGGTCTCCGGTACGCTGGAGGAACAGACAGCCAG
GACGGAGCGGGCCGCCATCGAATCGGCCCTGCAGGCTGCGGGTGGGAACCGGGCGCTGACCGCACGCATGCTTGGAATCC
ATCGCACCGCCTTATACAAAAAGATGCACCGACTGGGGATGGAATAG

Upstream 100 bases:

>100_bases
TATTTTAACGCGTGTAGACATATGGATACACACATCGCCCACCACCTCCGAAGAGATGGCGGTTTTGCAGCTATGAACCC
AAAAGGAGACATGTCGACTG

Downstream 100 bases:

>100_bases
CGACTATGGCTCCCCCAAACTTCCCAAAAGCTTGTATGGATTGGTTGCGTACGAGAAACAGGCAACGGTTCCCGAATAGT
CAAGAAGCGCCCTGAGCCGG

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MDTHTPASLFLKADYCRCGIVLLDESGRVTALNRQFFDTTGLEFREGDLPPDELRGLKKGEWIFWGRKWFVQAEGRHGVE
LLAIRRVRAADHILGPYGENCLNEEMVKMVLDNPYEGLTVVDATGKVTLLSPSNEQWLGLEVGGGRGLDLSAIAPASHLS
EVARTGVAEHAQVVDLHGKTKVTVNLPIRRDNKVMGAFGRILFKSTEQVEKLVTKVRTMELQVERFETLLDEMRGKRYSF
RNILTKNKEMLMLIEQARRVADSGATVLILGESGTGKELFAQALHEASRRNKGPFIAVNCGAIPRDLIESELFGYEEGAF
SGARKKGKPGKFELACGGTLFLDEIGELPLESQAKLLRVLEERKIDRLGGTAPLPVDFRLVAATNRDLMTNVNKGRFRAD
LYYRINEFPLEIPPLRSRPEDIPLLAKHFLTEVSRREELPMLKISAGATEALISHDWPGNVRELRGVMRQMAWKSQGLTI
EPHHLPPALNKGQTVGVSGTLEEQTARTERAAIESALQAAGGNRALTARMLGIHRTALYKKMHRLGME

Sequences:

>Translated_548_residues
MDTHTPASLFLKADYCRCGIVLLDESGRVTALNRQFFDTTGLEFREGDLPPDELRGLKKGEWIFWGRKWFVQAEGRHGVE
LLAIRRVRAADHILGPYGENCLNEEMVKMVLDNPYEGLTVVDATGKVTLLSPSNEQWLGLEVGGGRGLDLSAIAPASHLS
EVARTGVAEHAQVVDLHGKTKVTVNLPIRRDNKVMGAFGRILFKSTEQVEKLVTKVRTMELQVERFETLLDEMRGKRYSF
RNILTKNKEMLMLIEQARRVADSGATVLILGESGTGKELFAQALHEASRRNKGPFIAVNCGAIPRDLIESELFGYEEGAF
SGARKKGKPGKFELACGGTLFLDEIGELPLESQAKLLRVLEERKIDRLGGTAPLPVDFRLVAATNRDLMTNVNKGRFRAD
LYYRINEFPLEIPPLRSRPEDIPLLAKHFLTEVSRREELPMLKISAGATEALISHDWPGNVRELRGVMRQMAWKSQGLTI
EPHHLPPALNKGQTVGVSGTLEEQTARTERAAIESALQAAGGNRALTARMLGIHRTALYKKMHRLGME
>Mature_548_residues
MDTHTPASLFLKADYCRCGIVLLDESGRVTALNRQFFDTTGLEFREGDLPPDELRGLKKGEWIFWGRKWFVQAEGRHGVE
LLAIRRVRAADHILGPYGENCLNEEMVKMVLDNPYEGLTVVDATGKVTLLSPSNEQWLGLEVGGGRGLDLSAIAPASHLS
EVARTGVAEHAQVVDLHGKTKVTVNLPIRRDNKVMGAFGRILFKSTEQVEKLVTKVRTMELQVERFETLLDEMRGKRYSF
RNILTKNKEMLMLIEQARRVADSGATVLILGESGTGKELFAQALHEASRRNKGPFIAVNCGAIPRDLIESELFGYEEGAF
SGARKKGKPGKFELACGGTLFLDEIGELPLESQAKLLRVLEERKIDRLGGTAPLPVDFRLVAATNRDLMTNVNKGRFRAD
LYYRINEFPLEIPPLRSRPEDIPLLAKHFLTEVSRREELPMLKISAGATEALISHDWPGNVRELRGVMRQMAWKSQGLTI
EPHHLPPALNKGQTVGVSGTLEEQTARTERAAIESALQAAGGNRALTARMLGIHRTALYKKMHRLGME

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=342, Percent_Identity=45.3216374269006, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=46.875, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788905, Length=302, Percent_Identity=46.3576158940397, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1790437, Length=304, Percent_Identity=44.7368421052632, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=41.9354838709677, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1786524, Length=321, Percent_Identity=42.6791277258567, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1790299, Length=316, Percent_Identity=43.6708860759494, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1789233, Length=266, Percent_Identity=46.6165413533835, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082152, Length=311, Percent_Identity=44.6945337620579, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=39.6875, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=37.5796178343949, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI87081858, Length=331, Percent_Identity=35.6495468277946, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=36, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 61014; Mature: 61014

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4 ; PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHTPASLFLKADYCRCGIVLLDESGRVTALNRQFFDTTGLEFREGDLPPDELRGLKKG
CCCCCCHHEEEEECCCEEEEEEECCCCCEEEECCHHHCCCCCCEECCCCCHHHHCCCCCC
EWIFWGRKWFVQAEGRHGVELLAIRRVRAADHILGPYGENCLNEEMVKMVLDNPYEGLTV
CEEEEEEEEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEE
VDATGKVTLLSPSNEQWLGLEVGGGRGLDLSAIAPASHLSEVARTGVAEHAQVVDLHGKT
EECCCEEEEECCCCCCEEEEEECCCCCCCCHHCCCHHHHHHHHHCCHHHCCEEEEECCCE
KVTVNLPIRRDNKVMGAFGRILFKSTEQVEKLVTKVRTMELQVERFETLLDEMRGKRYSF
EEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RNILTKNKEMLMLIEQARRVADSGATVLILGESGTGKELFAQALHEASRRNKGPFIAVNC
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
GAIPRDLIESELFGYEEGAFSGARKKGKPGKFELACGGTLFLDEIGELPLESQAKLLRVL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCEEEHHHHCCCCCHHHHHHHHHH
EERKIDRLGGTAPLPVDFRLVAATNRDLMTNVNKGRFRADLYYRINEFPLEIPPLRSRPE
HHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCC
DIPLLAKHFLTEVSRREELPMLKISAGATEALISHDWPGNVRELRGVMRQMAWKSQGLTI
CCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEE
EPHHLPPALNKGQTVGVSGTLEEQTARTERAAIESALQAAGGNRALTARMLGIHRTALYK
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
KMHRLGME
HHHHHCCC
>Mature Secondary Structure
MDTHTPASLFLKADYCRCGIVLLDESGRVTALNRQFFDTTGLEFREGDLPPDELRGLKKG
CCCCCCHHEEEEECCCEEEEEEECCCCCEEEECCHHHCCCCCCEECCCCCHHHHCCCCCC
EWIFWGRKWFVQAEGRHGVELLAIRRVRAADHILGPYGENCLNEEMVKMVLDNPYEGLTV
CEEEEEEEEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEE
VDATGKVTLLSPSNEQWLGLEVGGGRGLDLSAIAPASHLSEVARTGVAEHAQVVDLHGKT
EECCCEEEEECCCCCCEEEEEECCCCCCCCHHCCCHHHHHHHHHCCHHHCCEEEEECCCE
KVTVNLPIRRDNKVMGAFGRILFKSTEQVEKLVTKVRTMELQVERFETLLDEMRGKRYSF
EEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RNILTKNKEMLMLIEQARRVADSGATVLILGESGTGKELFAQALHEASRRNKGPFIAVNC
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
GAIPRDLIESELFGYEEGAFSGARKKGKPGKFELACGGTLFLDEIGELPLESQAKLLRVL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCEEEHHHHCCCCCHHHHHHHHHH
EERKIDRLGGTAPLPVDFRLVAATNRDLMTNVNKGRFRADLYYRINEFPLEIPPLRSRPE
HHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCC
DIPLLAKHFLTEVSRREELPMLKISAGATEALISHDWPGNVRELRGVMRQMAWKSQGLTI
CCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEE
EPHHLPPALNKGQTVGVSGTLEEQTARTERAAIESALQAAGGNRALTARMLGIHRTALYK
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
KMHRLGME
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA