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The map label for this gene is purD [H]

Identifier: 78224100

GI number: 78224100

Start: 3286313

End: 3287584

Strand: Reverse

Name: purD [H]

Synonym: Gmet_2904

Alternate gene names: 78224100

Gene position: 3287584-3286313 (Counterclockwise)

Preceding gene: 78224101

Following gene: 78224099

Centisome position: 82.24

GC content: 65.02

Gene sequence:

>1272_bases
ATGAAAGTACTCGTTGTCGGCGGCGGCGGGCGGGAGCATGCCCTTGTCTGGAAGATTGCCCAGTCCCCTCTGGTTGGAAA
GGTCTACTGTGCCCCGGGTAACCCTGGCATCGGCGCCCTTGCCGAGAATGTCCCCCTGACGGTGGACGACCTCCCCGGCC
TCCTGGCCTTTGCCAAGGAAAAGGAAATCGGCCTTACGGTGGTGGGGCCGGAGTTGCCGCTCTCCCTCGGCATTGTCGAT
CTCTTTGAGGAACATGGCCTTCTGGTCTTCGGCGCCCGGAGGAACGCGGCCATCATCGAGGCGAGCAAGGCGTTCTCCAA
GGATCTCATGAAAAAGTACGGCGTCCCCACGGCCGCCTACGACGTCTTCACCGAGGTGGACCCGGCTGTCGCCTTCATCG
ACAAGGTCGGGGTCCCCATCGTCATCAAGGCAGACGGCCTGGCCGCCGGCAAGGGAGTCATCATCGCCCAGAGCCGAGAC
GAGGCTGTGGCAACGGTGAACGACATGCTCTCCGGCAACGCCTTCGGCTCGGCCGGTTCCCGGGTGGTGATCGAGGAGTT
CCTCGTGGGGGAGGAGGCTTCGTTCCTTGCCTTCACCGACGGAAAACAGATTATTCCTCTGGCCAGCGCCCAGGACCACA
AGGCGGTCTTCGACGGCGACCAGGGGCCCAACACCGGAGGGATGGGGGCCTACTCGCCTGCGCCGGTGGTTACCCAAACC
ATCCACGACTTCGCCATGGCCGAGGTGATGCGCCGGACCGTGGACGGCATGGCCGCCGAGGGGCGCCCCTACCGGGGGGT
TCTCTATGCGGGGCTCATGATCAACGGCAGCGACGTGAAGGTTCTGGAATTCAACGCCCGCTTCGGCGACCCCGAGTGCC
AACCGCTCCTCATGCGGATGAAGTCGGACATCGTGCCGGTGCTCCTCGGCGTGGCCAGGGGGGACCTGTCTGGGGTTGCC
ATCGAGTGGCACGACAAGGCGGCCGTCTGCGTTGTCATGGCTGCCGAGGGATATCCCGCCGACTACCGGAAGGGGGACGA
GATTCGCGGGCTCGACGAGGCTGCCGCCATCGATGACCTCTACGTTTTCCACGCCGGCACTGCTGCCGGGGACGGGAAGA
TCGTTACTGCCGGAGGGCGGGTGCTGGGGGTTACCGGCCTCGGCGCCACCGTGAAGGACGCCATCGATCGGGCCTACCGG
GGGGTCGCGAAGATCTGCTGGGAAGGGGTCCATTTCAGGAAGGATATCGGGGCAAAGGCGCTGAAGCGGTAG

Upstream 100 bases:

>100_bases
GCCGCTGCTGATGAGCACGGCATGGCCATGGTCTTCACGGGGATGAGGCATTTCAGGCACTAAGGCGAATATTACGAAAA
TCCACCACGGAGATTTTTTT

Downstream 100 bases:

>100_bases
CCGGCGCACGGAATCGAGACTGCATACACTAGGGGTACGTATGGGTAATCCACAGGTTCTTATTGTCATGGGGAGCGATT
CGGACCTCTCTATCATGGAG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVLVVGGGGREHALVWKIAQSPLVGKVYCAPGNPGIGALAENVPLTVDDLPGLLAFAKEKEIGLTVVGPELPLSLGIVD
LFEEHGLLVFGARRNAAIIEASKAFSKDLMKKYGVPTAAYDVFTEVDPAVAFIDKVGVPIVIKADGLAAGKGVIIAQSRD
EAVATVNDMLSGNAFGSAGSRVVIEEFLVGEEASFLAFTDGKQIIPLASAQDHKAVFDGDQGPNTGGMGAYSPAPVVTQT
IHDFAMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGSDVKVLEFNARFGDPECQPLLMRMKSDIVPVLLGVARGDLSGVA
IEWHDKAAVCVVMAAEGYPADYRKGDEIRGLDEAAAIDDLYVFHAGTAAGDGKIVTAGGRVLGVTGLGATVKDAIDRAYR
GVAKICWEGVHFRKDIGAKALKR

Sequences:

>Translated_423_residues
MKVLVVGGGGREHALVWKIAQSPLVGKVYCAPGNPGIGALAENVPLTVDDLPGLLAFAKEKEIGLTVVGPELPLSLGIVD
LFEEHGLLVFGARRNAAIIEASKAFSKDLMKKYGVPTAAYDVFTEVDPAVAFIDKVGVPIVIKADGLAAGKGVIIAQSRD
EAVATVNDMLSGNAFGSAGSRVVIEEFLVGEEASFLAFTDGKQIIPLASAQDHKAVFDGDQGPNTGGMGAYSPAPVVTQT
IHDFAMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGSDVKVLEFNARFGDPECQPLLMRMKSDIVPVLLGVARGDLSGVA
IEWHDKAAVCVVMAAEGYPADYRKGDEIRGLDEAAAIDDLYVFHAGTAAGDGKIVTAGGRVLGVTGLGATVKDAIDRAYR
GVAKICWEGVHFRKDIGAKALKR
>Mature_423_residues
MKVLVVGGGGREHALVWKIAQSPLVGKVYCAPGNPGIGALAENVPLTVDDLPGLLAFAKEKEIGLTVVGPELPLSLGIVD
LFEEHGLLVFGARRNAAIIEASKAFSKDLMKKYGVPTAAYDVFTEVDPAVAFIDKVGVPIVIKADGLAAGKGVIIAQSRD
EAVATVNDMLSGNAFGSAGSRVVIEEFLVGEEASFLAFTDGKQIIPLASAQDHKAVFDGDQGPNTGGMGAYSPAPVVTQT
IHDFAMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGSDVKVLEFNARFGDPECQPLLMRMKSDIVPVLLGVARGDLSGVA
IEWHDKAAVCVVMAAEGYPADYRKGDEIRGLDEAAAIDDLYVFHAGTAAGDGKIVTAGGRVLGVTGLGATVKDAIDRAYR
GVAKICWEGVHFRKDIGAKALKR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=48.8207547169811, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=48.8207547169811, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=48.8207547169811, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=48.8207547169811, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=56.1771561771562, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=42.4528301886792, Blast_Score=291, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=43.6651583710407, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=43.3874709976798, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=43.3874709976798, Blast_Score=313, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44376; Mature: 44376

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGGGGREHALVWKIAQSPLVGKVYCAPGNPGIGALAENVPLTVDDLPGLLAFAKE
CEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHCCCCEEHHCCCCHHHHHCC
KEIGLTVVGPELPLSLGIVDLFEEHGLLVFGARRNAAIIEASKAFSKDLMKKYGVPTAAY
CCCCEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCHHHH
DVFTEVDPAVAFIDKVGVPIVIKADGLAAGKGVIIAQSRDEAVATVNDMLSGNAFGSAGS
HHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCC
RVVIEEFLVGEEASFLAFTDGKQIIPLASAQDHKAVFDGDQGPNTGGMGAYSPAPVVTQT
EEEEHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHH
IHDFAMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGSDVKVLEFNARFGDPECQPLLMRM
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHH
KSDIVPVLLGVARGDLSGVAIEWHDKAAVCVVMAAEGYPADYRKGDEIRGLDEAAAIDDL
HHCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCEE
YVFHAGTAAGDGKIVTAGGRVLGVTGLGATVKDAIDRAYRGVAKICWEGVHFRKDIGAKA
EEEECCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHH
LKR
HCC
>Mature Secondary Structure
MKVLVVGGGGREHALVWKIAQSPLVGKVYCAPGNPGIGALAENVPLTVDDLPGLLAFAKE
CEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHCCCCEEHHCCCCHHHHHCC
KEIGLTVVGPELPLSLGIVDLFEEHGLLVFGARRNAAIIEASKAFSKDLMKKYGVPTAAY
CCCCEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCHHHH
DVFTEVDPAVAFIDKVGVPIVIKADGLAAGKGVIIAQSRDEAVATVNDMLSGNAFGSAGS
HHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCC
RVVIEEFLVGEEASFLAFTDGKQIIPLASAQDHKAVFDGDQGPNTGGMGAYSPAPVVTQT
EEEEHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHH
IHDFAMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGSDVKVLEFNARFGDPECQPLLMRM
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHH
KSDIVPVLLGVARGDLSGVAIEWHDKAAVCVVMAAEGYPADYRKGDEIRGLDEAAAIDDL
HHCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCEE
YVFHAGTAAGDGKIVTAGGRVLGVTGLGATVKDAIDRAYRGVAKICWEGVHFRKDIGAKA
EEEECCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHH
LKR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]