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The map label for this gene is purH

Identifier: 78224101

GI number: 78224101

Start: 3287622

End: 3289187

Strand: Reverse

Name: purH

Synonym: Gmet_2905

Alternate gene names: 78224101

Gene position: 3289187-3287622 (Counterclockwise)

Preceding gene: 78224102

Following gene: 78224100

Centisome position: 82.28

GC content: 65.2

Gene sequence:

>1566_bases
ATGGCAAAGATTACCCGTGCGCTTATCAGTCTTTCCGACAAAACCGGCATTGTCGAGTTCGCCAGGGAACTGGCCGGCTA
CGGCGTCGAGATCCTCTCCACCGGCGGCACCGCAAAGCTGCTCCGGGATGCCGGCCTGACGGTGAAGGACGTGTCCGAGC
ACACCGGCTTCCCCGAGATGCTGGACGGCCGGGTGAAGACCCTCCATCCGAAGGTCCACGGCGGGCTCCTTGGCATGCGC
TCCAACCCGGAGCACGTGGCCACCATGAAGGAACACGGCATCCTCCCCATCGACATGGTGGTGGTGAACCTCTACCCCTT
CGAGGCCACCGTGGCCAATCCCGCCTGCACCCTTGAGGACGCCATCGAGAACATCGATATCGGCGGCCCCACCATGCTCC
GCTCCGCGGCCAAGAACAACGCCGACGTGACGGTCGTTGTGGACCCGGCCGACTACCGGACGGTCCTTGACGAGATGAAG
GCATCGGGCGGCTCCGTTGCCAAGGAGACCAACTTCCGCCTGGCGGTGAAGGTCTACCAGCACACCGCTGCCTACGATGG
CGCCATCTCCAACTGGCTCGGCGCCCGGACCGGGGAGGGGGTCGCTACCTATTCCGACACCATTACCCTCCAGTTCAAGA
AGGCCCAGGAGATGCGCTACGGCGAAAACCCCCACCAATCGGCCGCCTTCTACGTGGAGCGGCAGGTGCAGGAGGCGTCG
GTTTCTACGGCCCGCCAGCTCCAGGGGAAGGAACTCTCCTACAACAACATCGGCGACACCGACGCGGCCCTGGAGTGCGT
GAAGCAGTTCAGCGAAGGGCCCGCCTGCGTCATTGTCAAGCACGCGAACCCCTGCGGCGTGGCCATCGGGAAGAACCTCC
TGGAGGCCTACGACCGGGCCTACGCCACCGACCCCGAATCGGCCTTCGGCGGCATCATCGCCTTCAATGGCGAGTTGGAC
GCCGAGACGGCCAAGGCCATCTGTGACCGCCAGTTCGTGGAGGTGATCATCGCCCCCTCAGTTTCCAAAGCGGCCGTGGA
GGTGGTGGCGGCCAAGAAAAACGTCCGGCTCCTGGAGTGCGGCATATGGCCGGCCCAGCCGGCCCAGCGCCTCGATTTCA
AGCGGGTGAACGGCGGGCTTCTGGTCCAGGACACTGACTTGGCCCTCTCGGCCGAGTTGAAGGTGGTGTCGAAGCGCCAG
CCCACGGCGCAGGAGATGATCGACCTCCAGTTCGCCTGGCGGGTGGCCAAGTTCGTGAAGTCCAATGCCATCGTCTATGG
CAAGGACGGCATGACCATCGGCGTCGGGGCCGGCCAGATGAGCCGGGTCAACTCGGCCCGCATCGCCGCCATCAAGGCGG
AGCACGCGGGGCTTGAGGTGCAGGGGGCGGTCATGGCCTCCGATGCCTTCTTCCCCTTCCGCGACGGCATCGACAACGCC
GCCGCCGTGGGGATCACGGCGGTCATCCAGCCCGGGGGAAGCATGCGCGACGCCGAGGTGATCGCCGCTGCTGATGAGCA
CGGCATGGCCATGGTCTTCACGGGGATGAGGCATTTCAGGCACTAA

Upstream 100 bases:

>100_bases
TTTCCGGCACCCCAGCCTTCGAGAAGCACTTTCTGCAGCATATCAATTTCCCGCATACAATCTGATCGATTCATCAATTC
AATTCTCAAGGAGTGACACC

Downstream 100 bases:

>100_bases
GGCGAATATTACGAAAATCCACCACGGAGATTTTTTTATGAAAGTACTCGTTGTCGGCGGCGGCGGGCGGGAGCATGCCC
TTGTCTGGAAGATTGCCCAG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MAKITRALISLSDKTGIVEFARELAGYGVEILSTGGTAKLLRDAGLTVKDVSEHTGFPEMLDGRVKTLHPKVHGGLLGMR
SNPEHVATMKEHGILPIDMVVVNLYPFEATVANPACTLEDAIENIDIGGPTMLRSAAKNNADVTVVVDPADYRTVLDEMK
ASGGSVAKETNFRLAVKVYQHTAAYDGAISNWLGARTGEGVATYSDTITLQFKKAQEMRYGENPHQSAAFYVERQVQEAS
VSTARQLQGKELSYNNIGDTDAALECVKQFSEGPACVIVKHANPCGVAIGKNLLEAYDRAYATDPESAFGGIIAFNGELD
AETAKAICDRQFVEVIIAPSVSKAAVEVVAAKKNVRLLECGIWPAQPAQRLDFKRVNGGLLVQDTDLALSAELKVVSKRQ
PTAQEMIDLQFAWRVAKFVKSNAIVYGKDGMTIGVGAGQMSRVNSARIAAIKAEHAGLEVQGAVMASDAFFPFRDGIDNA
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH

Sequences:

>Translated_521_residues
MAKITRALISLSDKTGIVEFARELAGYGVEILSTGGTAKLLRDAGLTVKDVSEHTGFPEMLDGRVKTLHPKVHGGLLGMR
SNPEHVATMKEHGILPIDMVVVNLYPFEATVANPACTLEDAIENIDIGGPTMLRSAAKNNADVTVVVDPADYRTVLDEMK
ASGGSVAKETNFRLAVKVYQHTAAYDGAISNWLGARTGEGVATYSDTITLQFKKAQEMRYGENPHQSAAFYVERQVQEAS
VSTARQLQGKELSYNNIGDTDAALECVKQFSEGPACVIVKHANPCGVAIGKNLLEAYDRAYATDPESAFGGIIAFNGELD
AETAKAICDRQFVEVIIAPSVSKAAVEVVAAKKNVRLLECGIWPAQPAQRLDFKRVNGGLLVQDTDLALSAELKVVSKRQ
PTAQEMIDLQFAWRVAKFVKSNAIVYGKDGMTIGVGAGQMSRVNSARIAAIKAEHAGLEVQGAVMASDAFFPFRDGIDNA
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH
>Mature_520_residues
AKITRALISLSDKTGIVEFARELAGYGVEILSTGGTAKLLRDAGLTVKDVSEHTGFPEMLDGRVKTLHPKVHGGLLGMRS
NPEHVATMKEHGILPIDMVVVNLYPFEATVANPACTLEDAIENIDIGGPTMLRSAAKNNADVTVVVDPADYRTVLDEMKA
SGGSVAKETNFRLAVKVYQHTAAYDGAISNWLGARTGEGVATYSDTITLQFKKAQEMRYGENPHQSAAFYVERQVQEASV
STARQLQGKELSYNNIGDTDAALECVKQFSEGPACVIVKHANPCGVAIGKNLLEAYDRAYATDPESAFGGIIAFNGELDA
ETAKAICDRQFVEVIIAPSVSKAAVEVVAAKKNVRLLECGIWPAQPAQRLDFKRVNGGLLVQDTDLALSAELKVVSKRQP
TAQEMIDLQFAWRVAKFVKSNAIVYGKDGMTIGVGAGQMSRVNSARIAAIKAEHAGLEVQGAVMASDAFFPFRDGIDNAA
AVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=515, Percent_Identity=38.8349514563107, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=66.2239089184061, Blast_Score=703, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=503, Percent_Identity=35.5864811133201, Blast_Score=254, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=333, Percent_Identity=28.8288288288288, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71985556, Length=146, Percent_Identity=34.2465753424658, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=38.477366255144, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6323056, Length=497, Percent_Identity=36.6197183098592, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649832, Length=508, Percent_Identity=37.5984251968504, Blast_Score=282, Evalue=5e-76,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_GEOMG (Q39RK1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385848.1
- HSSP:   P31335
- ProteinModelPortal:   Q39RK1
- SMR:   Q39RK1
- STRING:   Q39RK1
- GeneID:   3740855
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2905
- NMPDR:   fig|269799.3.peg.3075
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   Q39RK1
- ProtClustDB:   PRK00881
- BioCyc:   GMET269799:GMET_2905-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55783; Mature: 55652

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITRALISLSDKTGIVEFARELAGYGVEILSTGGTAKLLRDAGLTVKDVSEHTGFPEM
CCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEECHHHCCCHHH
LDGRVKTLHPKVHGGLLGMRSNPEHVATMKEHGILPIDMVVVNLYPFEATVANPACTLED
HCCCCEEECCHHCCCEECCCCCCHHHHHHHHCCCCEEEEEEEEEECCEEECCCCCCCHHH
AIENIDIGGPTMLRSAAKNNADVTVVVDPADYRTVLDEMKASGGSVAKETNFRLAVKVYQ
HHHHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCEECCCCEEEEEEEH
HTAAYDGAISNWLGARTGEGVATYSDTITLQFKKAQEMRYGENPHQSAAFYVERQVQEAS
HHHHHCCHHHHHHCCCCCCCEEEECCEEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHH
VSTARQLQGKELSYNNIGDTDAALECVKQFSEGPACVIVKHANPCGVAIGKNLLEAYDRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
YATDPESAFGGIIAFNGELDAETAKAICDRQFVEVIIAPSVSKAAVEVVAAKKNVRLLEC
CCCCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEE
GIWPAQPAQRLDFKRVNGGLLVQDTDLALSAELKVVSKRQPTAQEMIDLQFAWRVAKFVK
CCCCCCHHHHCCCEECCCCEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SNAIVYGKDGMTIGVGAGQMSRVNSARIAAIKAEHAGLEVQGAVMASDAFFPFRDGIDNA
CCCEEECCCCCEEEECCCCHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHCCCCCH
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH
HHHEEEEEECCCCCCCCCEEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
AKITRALISLSDKTGIVEFARELAGYGVEILSTGGTAKLLRDAGLTVKDVSEHTGFPEM
CHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEECHHHCCCHHH
LDGRVKTLHPKVHGGLLGMRSNPEHVATMKEHGILPIDMVVVNLYPFEATVANPACTLED
HCCCCEEECCHHCCCEECCCCCCHHHHHHHHCCCCEEEEEEEEEECCEEECCCCCCCHHH
AIENIDIGGPTMLRSAAKNNADVTVVVDPADYRTVLDEMKASGGSVAKETNFRLAVKVYQ
HHHHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCEECCCCEEEEEEEH
HTAAYDGAISNWLGARTGEGVATYSDTITLQFKKAQEMRYGENPHQSAAFYVERQVQEAS
HHHHHCCHHHHHHCCCCCCCEEEECCEEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHH
VSTARQLQGKELSYNNIGDTDAALECVKQFSEGPACVIVKHANPCGVAIGKNLLEAYDRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
YATDPESAFGGIIAFNGELDAETAKAICDRQFVEVIIAPSVSKAAVEVVAAKKNVRLLEC
CCCCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEE
GIWPAQPAQRLDFKRVNGGLLVQDTDLALSAELKVVSKRQPTAQEMIDLQFAWRVAKFVK
CCCCCCHHHHCCCEECCCCEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SNAIVYGKDGMTIGVGAGQMSRVNSARIAAIKAEHAGLEVQGAVMASDAFFPFRDGIDNA
CCCEEECCCCCEEEECCCCHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHCCCCCH
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH
HHHEEEEEECCCCCCCCCEEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA