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The map label for this gene is gltA [H]

Identifier: 78223885

GI number: 78223885

Start: 3035314

End: 3036639

Strand: Reverse

Name: gltA [H]

Synonym: Gmet_2689

Alternate gene names: 78223885

Gene position: 3036639-3035314 (Counterclockwise)

Preceding gene: 78223886

Following gene: 78223883

Centisome position: 75.96

GC content: 57.54

Gene sequence:

>1326_bases
ATGGCACTCAAGGAAACCCTGAAGCAAAAAATTGAGGAGTTCCGTCCCCGTACCACCAGACTTGTGAAAGAGTTTGGGAA
AGTGGTTATCGACCAGGTAACAATTGATCAGGCCATTGGCGGTGCCCGTGACATCCGCAGCCTGGTAACCGATATCTCCT
ACCTCGACCCGCAGGAAGGTATCCGGTTCCGCGGCAAGACCATTCCCGAGACCTTTGAGGCGCTCCCCAAGGCCTCCGGC
TCTGATTATCCGACCGTTGAGTCCTTCTGGTACTTCCTCCTTACCGGCGAGGTTCCGACTCAAGCCCAGGTTGACGAGGT
GGTTGCTGAGTGGAAGACCCGTCAGGTAGTCCCCCAGTATGTATTTGACGCAATCAGCGCCCTGCCGAAGGAAAGCCACC
CGATGGTGATGCTGTCGGTCGGCATCCTCGCCCTGCAGAAGGATTCCAAATTTGCCGGTTTCTACAACTCTGGCAAGTTT
AACAAGATGACTGCCTGGGAATACGTTTATGAAGATGCCAGCGATCTCGTTGCCCGTATCCCCATCATCGCCGCGTTCAT
TTACAACCTCAAGTACAGGGGTGGCAAGCAGAGTGCCATAGACCCGACTGCAGACTGCGGCGCCAACTTCGCCCGCATGA
TCGGTCAGTGCAAGGAGTACGAGGACGTTGCCCGGATGTACTTCATCCTTCACTCCGACCACGAATCCGGCAACGTTTCA
GCGCACACCACTCACTTGGTACACTCTGCCCTGTCCGACCCCTACTATGCATACTCTGCCGGTCTTAACGGCCTTGCCGG
TCCGCTGCACGGCCTTGCCAACCAGGAAGTCCTCGACTGGACCATCAAGTTCCAGCAGAAGTACTGCAAGGATGTGGAGC
CGACCAAGGAACTGGTCACCAAGGCCCTCTGGGATACCCTCAATGCCGGCCAGGTCGTACCGGGCTACGGCCACGCCGTT
CTCCGCAAGACCGACCCGCGCTACATGTCGCAGCGTGAGTTCTGCCTCAAGACCCCGGGTCTCAAAGATGATCCGCTCTT
CAAGCTTGTTGCCATGATCTTTGAGACCGCCCCGGGCGTCCTCATGGAGCACGGCAAGGCCAAGAACCCCTGGCCGAACG
TTGATGCCCAGTCCGGTGTCATCCAGTGGTACTATGGCCTCCGTGAGTGGGACTTCTACACCGTCCTCTTCGGTGTAGGC
CGCGCTCTCGGTTGCATGGCGAACATCACTTGGGACCGTGGTCTCGGCTACGCCATCGAGCGTCCGAAGTCCGTTACCAC
CCCGATGCTTGAGAAGTGGGCTGAAGAAGGTGGCCGGAAGTTCTAA

Upstream 100 bases:

>100_bases
GTATACTACCAGTGGAAAGGAGGGGGAAGAAAAGGTAGTCGAGGTTTATGGTTTGTTTTTGTTTATTAAAACACTATAAC
GTGAAAGGAGAGTACGTAAC

Downstream 100 bases:

>100_bases
TACTTCCTGTGTGCGGGAGTCTCCCAAGGACTCCCGCATGTAGCCAGAAGTGCAGAAAAAGGGGAGATGTCTAGACATCT
CCCCCTTTTCGTATGTGCGG

Product: type I citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MALKETLKQKIEEFRPRTTRLVKEFGKVVIDQVTIDQAIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFEALPKASG
SDYPTVESFWYFLLTGEVPTQAQVDEVVAEWKTRQVVPQYVFDAISALPKESHPMVMLSVGILALQKDSKFAGFYNSGKF
NKMTAWEYVYEDASDLVARIPIIAAFIYNLKYRGGKQSAIDPTADCGANFARMIGQCKEYEDVARMYFILHSDHESGNVS
AHTTHLVHSALSDPYYAYSAGLNGLAGPLHGLANQEVLDWTIKFQQKYCKDVEPTKELVTKALWDTLNAGQVVPGYGHAV
LRKTDPRYMSQREFCLKTPGLKDDPLFKLVAMIFETAPGVLMEHGKAKNPWPNVDAQSGVIQWYYGLREWDFYTVLFGVG
RALGCMANITWDRGLGYAIERPKSVTTPMLEKWAEEGGRKF

Sequences:

>Translated_441_residues
MALKETLKQKIEEFRPRTTRLVKEFGKVVIDQVTIDQAIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFEALPKASG
SDYPTVESFWYFLLTGEVPTQAQVDEVVAEWKTRQVVPQYVFDAISALPKESHPMVMLSVGILALQKDSKFAGFYNSGKF
NKMTAWEYVYEDASDLVARIPIIAAFIYNLKYRGGKQSAIDPTADCGANFARMIGQCKEYEDVARMYFILHSDHESGNVS
AHTTHLVHSALSDPYYAYSAGLNGLAGPLHGLANQEVLDWTIKFQQKYCKDVEPTKELVTKALWDTLNAGQVVPGYGHAV
LRKTDPRYMSQREFCLKTPGLKDDPLFKLVAMIFETAPGVLMEHGKAKNPWPNVDAQSGVIQWYYGLREWDFYTVLFGVG
RALGCMANITWDRGLGYAIERPKSVTTPMLEKWAEEGGRKF
>Mature_440_residues
ALKETLKQKIEEFRPRTTRLVKEFGKVVIDQVTIDQAIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFEALPKASGS
DYPTVESFWYFLLTGEVPTQAQVDEVVAEWKTRQVVPQYVFDAISALPKESHPMVMLSVGILALQKDSKFAGFYNSGKFN
KMTAWEYVYEDASDLVARIPIIAAFIYNLKYRGGKQSAIDPTADCGANFARMIGQCKEYEDVARMYFILHSDHESGNVSA
HTTHLVHSALSDPYYAYSAGLNGLAGPLHGLANQEVLDWTIKFQQKYCKDVEPTKELVTKALWDTLNAGQVVPGYGHAVL
RKTDPRYMSQREFCLKTPGLKDDPLFKLVAMIFETAPGVLMEHGKAKNPWPNVDAQSGVIQWYYGLREWDFYTVLFGVGR
ALGCMANITWDRGLGYAIERPKSVTTPMLEKWAEEGGRKF

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=435, Percent_Identity=49.6551724137931, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI1786939, Length=397, Percent_Identity=30.4785894206549, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786527, Length=343, Percent_Identity=25.0728862973761, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555174, Length=436, Percent_Identity=50.2293577981651, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6324328, Length=439, Percent_Identity=52.8473804100228, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319850, Length=437, Percent_Identity=51.0297482837529, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6325257, Length=451, Percent_Identity=39.689578713969, Blast_Score=327, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24640126, Length=437, Percent_Identity=51.0297482837529, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24640124, Length=437, Percent_Identity=51.0297482837529, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21356863, Length=439, Percent_Identity=43.5079726651481, Blast_Score=357, Evalue=6e-99,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 49633; Mature: 49502

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKETLKQKIEEFRPRTTRLVKEFGKVVIDQVTIDQAIGGARDIRSLVTDISYLDPQEG
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHC
IRFRGKTIPETFEALPKASGSDYPTVESFWYFLLTGEVPTQAQVDEVVAEWKTRQVVPQY
CEECCCCCHHHHHHHCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFDAISALPKESHPMVMLSVGILALQKDSKFAGFYNSGKFNKMTAWEYVYEDASDLVARI
HHHHHHHCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PIIAAFIYNLKYRGGKQSAIDPTADCGANFARMIGQCKEYEDVARMYFILHSDHESGNVS
HHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
AHTTHLVHSALSDPYYAYSAGLNGLAGPLHGLANQEVLDWTIKFQQKYCKDVEPTKELVT
HHHHHHHHHHHCCCCEEHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
KALWDTLNAGQVVPGYGHAVLRKTDPRYMSQREFCLKTPGLKDDPLFKLVAMIFETAPGV
HHHHHHCCCCCCCCCCCCHHEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCH
LMEHGKAKNPWPNVDAQSGVIQWYYGLREWDFYTVLFGVGRALGCMANITWDRGLGYAIE
HHHCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHC
RPKSVTTPMLEKWAEEGGRKF
CCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALKETLKQKIEEFRPRTTRLVKEFGKVVIDQVTIDQAIGGARDIRSLVTDISYLDPQEG
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHC
IRFRGKTIPETFEALPKASGSDYPTVESFWYFLLTGEVPTQAQVDEVVAEWKTRQVVPQY
CEECCCCCHHHHHHHCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFDAISALPKESHPMVMLSVGILALQKDSKFAGFYNSGKFNKMTAWEYVYEDASDLVARI
HHHHHHHCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PIIAAFIYNLKYRGGKQSAIDPTADCGANFARMIGQCKEYEDVARMYFILHSDHESGNVS
HHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
AHTTHLVHSALSDPYYAYSAGLNGLAGPLHGLANQEVLDWTIKFQQKYCKDVEPTKELVT
HHHHHHHHHHHCCCCEEHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
KALWDTLNAGQVVPGYGHAVLRKTDPRYMSQREFCLKTPGLKDDPLFKLVAMIFETAPGV
HHHHHHCCCCCCCCCCCCHHEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCH
LMEHGKAKNPWPNVDAQSGVIQWYYGLREWDFYTVLFGVGRALGCMANITWDRGLGYAIE
HHHCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHC
RPKSVTTPMLEKWAEEGGRKF
CCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7522844; 12948626 [H]