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The map label for this gene is hyfE [C]

Identifier: 78223796

GI number: 78223796

Start: 2949398

End: 2950039

Strand: Reverse

Name: hyfE [C]

Synonym: Gmet_2599

Alternate gene names: 78223796

Gene position: 2950039-2949398 (Counterclockwise)

Preceding gene: 78223797

Following gene: 78223795

Centisome position: 73.8

GC content: 59.19

Gene sequence:

>642_bases
ATGAGTTCATTCGCCGATCAACTGCTGGTACTGGTGATGCTGATCAATCTGATCATGCTTGGCACGAGCAGGCTGATGTT
TTCCATCCGTGCCGTTGCCGCCCAGGGGGTCATCCTCGGTATCCTCCCCGGCTTGATACACCCGTTCTCCGGACACCTGG
CCAGCATCACCGTCGGCATCATCCTGGCCAAGGGAGTGGCGATTCCCTACTTCATCGGAGGCGCCGTCCGCAAGGCCCAG
ATCAGGCGCGAAGTTGAACCCTTCATCGGGTATGTGCCGACCTTGCTCCTCGGTGCCGTTTTCACCGCCCTCTCGTTTGT
CTTTGCCGAACGCCTGCCTCTGGCGCCCGAACATAGGGATCTCCTGTTTGTGCCGGCCTCCATTGCCACGCTGCTGTGCG
GTTTTCTGATCCTGACGACCCGCCGCAAGGCCATTTCCCAGGTCATCGGCTATCTGGTGATGGAGAACGGGATATTCGTA
TTCGGTCTTCTCCTGACCGAGGCGATGCCGGTCATGGTTGAGGCCGGCGCCCTGCTCGACCTTCTCGTGGGGATATTCGT
CATGGGTATTGTCATCAACCATATCAGCCGGGAATTCTCGTCGATCGACACCTCCCGCCTGCGGGCCCTGAAGGAGGAGT
AG

Upstream 100 bases:

>100_bases
TCGGTGATGGCCCGGTTGCGTCTGGTCCGCGTTCCGCAACTGCTGGTCGGAGCCACTATCCTGTCCGCCTTTGCCATGCT
GCTGGTATTGAGGTAGCCCC

Downstream 100 bases:

>100_bases
CCTCACATGTTGTATGCCCTTGTTCTGCTCCCCCTGGTGGGGGCGTTATGCGCCTGGATCGTGCCGGACAATCGTCTCAG
GCCCATTACCCTGCCGGCTG

Product: NAD-dependent dehydrogenase subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSSFADQLLVLVMLINLIMLGTSRLMFSIRAVAAQGVILGILPGLIHPFSGHLASITVGIILAKGVAIPYFIGGAVRKAQ
IRREVEPFIGYVPTLLLGAVFTALSFVFAERLPLAPEHRDLLFVPASIATLLCGFLILTTRRKAISQVIGYLVMENGIFV
FGLLLTEAMPVMVEAGALLDLLVGIFVMGIVINHISREFSSIDTSRLRALKEE

Sequences:

>Translated_213_residues
MSSFADQLLVLVMLINLIMLGTSRLMFSIRAVAAQGVILGILPGLIHPFSGHLASITVGIILAKGVAIPYFIGGAVRKAQ
IRREVEPFIGYVPTLLLGAVFTALSFVFAERLPLAPEHRDLLFVPASIATLLCGFLILTTRRKAISQVIGYLVMENGIFV
FGLLLTEAMPVMVEAGALLDLLVGIFVMGIVINHISREFSSIDTSRLRALKEE
>Mature_212_residues
SSFADQLLVLVMLINLIMLGTSRLMFSIRAVAAQGVILGILPGLIHPFSGHLASITVGIILAKGVAIPYFIGGAVRKAQI
RREVEPFIGYVPTLLLGAVFTALSFVFAERLPLAPEHRDLLFVPASIATLLCGFLILTTRRKAISQVIGYLVMENGIFVF
GLLLTEAMPVMVEAGALLDLLVGIFVMGIVINHISREFSSIDTSRLRALKEE

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23060; Mature: 22929

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFADQLLVLVMLINLIMLGTSRLMFSIRAVAAQGVILGILPGLIHPFSGHLASITVGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHH
ILAKGVAIPYFIGGAVRKAQIRREVEPFIGYVPTLLLGAVFTALSFVFAERLPLAPEHRD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LLFVPASIATLLCGFLILTTRRKAISQVIGYLVMENGIFVFGLLLTEAMPVMVEAGALLD
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLVGIFVMGIVINHISREFSSIDTSRLRALKEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
SSFADQLLVLVMLINLIMLGTSRLMFSIRAVAAQGVILGILPGLIHPFSGHLASITVGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHH
ILAKGVAIPYFIGGAVRKAQIRREVEPFIGYVPTLLLGAVFTALSFVFAERLPLAPEHRD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LLFVPASIATLLCGFLILTTRRKAISQVIGYLVMENGIFVFGLLLTEAMPVMVEAGALLD
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLVGIFVMGIVINHISREFSSIDTSRLRALKEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]