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The map label for this gene is hyfF [H]

Identifier: 78223795

GI number: 78223795

Start: 2947955

End: 2949391

Strand: Reverse

Name: hyfF [H]

Synonym: Gmet_2598

Alternate gene names: 78223795

Gene position: 2949391-2947955 (Counterclockwise)

Preceding gene: 78223796

Following gene: 78223794

Centisome position: 73.78

GC content: 62.84

Gene sequence:

>1437_bases
ATGTTGTATGCCCTTGTTCTGCTCCCCCTGGTGGGGGCGTTATGCGCCTGGATCGTGCCGGACAATCGTCTCAGGCCCAT
TACCCTGCCGGCTGTTGCGACGGTTCACCTGGCACTGACGGCGCTATTGCTGGTGAACACGCCGCCACCATCCCCCGGAG
GCTGGATCTGGCTCGACCCCCTGGGGAAGGTGGTGCTCCTGTGCATCAGCCTCCTCTTCACCGTCTGCGCCTATTATGCC
GTCGGCTACCTCTCCTACCGTCAGGAGCGTTCGAACCGGGTCCTCTGCATGGGGCTGCTGGTCTGCCTTTCGGCCATGGG
GCTGGTCACCATGACCCAGCATCTGGGACTGCTCTGGGTCGCACTGGAAACAACCACCGTGTCCATGGCCCCCCTCATCT
ACTTCAATCGCAATGCCCGCTCCATAGAGGCCACCTGGAAGTATCTGCTCCTCTGTTCCGTTGGCATCGGCCTGGCGTTG
CTCGGGCTGCTGTTCCTCGCCTATTCCACCTACGTGGCCCACCGGGACGCCACCCTGCTGCTCGGCCCGCTGATGCAGTC
CGCCCGTGAGCTGAACCCCGGCTGGCTCCACGCGGCGGCGATTTTTCTCCTGGTGGGGTATGGTTCAAAGATGGGGCTGG
CCCCGCTCCATGCCTGGAAGCCCGATGCCTATGGCGAAGCGCCCGGCCTGGTGGGGGCCTTGCTTGCCGGGGGGCTGGTC
AACTGCGCATTTCTGTGCATCATGCGGGTTTATCAGATCTGCCTGGCATCCGGTGCCGAACTGGCGTTCTTTCAAGATGC
CCTCGTCGGCATGGGGCTCATCTCCATGGCCTTTGCCGCCGTATTCATGGCGCACCAGGCCGACTTCAAGAGGATGCTGG
CCTATTCGAGCGTCGAACATGTGGGGATTCTCGCCATCGGTCTCGGGCTTGGCAAGGGGGCGCTGTACGGCGCGCTCTTT
CATCTCCTGAACAACGGGCTGACCAAGGGGGTGCTGTTCCTCTCCTCCGGCAACATCCACCGGTCCTACGGAAACAAGAC
GACCGATGTGGTCAGGGGGGCACTGGCACGGCTCCCCTGGTCGGGGGGGCTCTTTCTGGCCGGTTTCATTGCCATTACCG
GTTCACCGCCGTTCTCGCCGTTCATCAGTGAGTATGCGATTGTCAGCAACGCCTTCATCGACGGGCGGCATCTGGTGGGG
TGGCTGTTCCTGCTGTTTCTGGTCATTGTGTTCATCGGCATGGCCCTGACGGTGCTGCCGATGGTCATGGGTACCCCACC
CGCCGGCCTCGAACCCTCCGATTACGAGGACCGTCTGCTGACCGTGGGGCCGCCGTTTCTCATGATGGCGGTCATCCTGC
TGCTGGGGATATGGCCGCCTGCTTTCCTTGTCTCGCTCCTGACAGAGGGCGCTGCGATGCTGGGGGTGCAACCGTGA

Upstream 100 bases:

>100_bases
GGGGATATTCGTCATGGGTATTGTCATCAACCATATCAGCCGGGAATTCTCGTCGATCGACACCTCCCGCCTGCGGGCCC
TGAAGGAGGAGTAGCCTCAC

Downstream 100 bases:

>100_bases
AGGATTCATTCCGCACCATATACAACGGGGCCTCCGTCCCCCTGGCAGAGATTCCCCCCGTAGATATCAACGAGTTTTTG
CAGGGGATTCTCGATTCCGT

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLYALVLLPLVGALCAWIVPDNRLRPITLPAVATVHLALTALLLVNTPPPSPGGWIWLDPLGKVVLLCISLLFTVCAYYA
VGYLSYRQERSNRVLCMGLLVCLSAMGLVTMTQHLGLLWVALETTTVSMAPLIYFNRNARSIEATWKYLLLCSVGIGLAL
LGLLFLAYSTYVAHRDATLLLGPLMQSARELNPGWLHAAAIFLLVGYGSKMGLAPLHAWKPDAYGEAPGLVGALLAGGLV
NCAFLCIMRVYQICLASGAELAFFQDALVGMGLISMAFAAVFMAHQADFKRMLAYSSVEHVGILAIGLGLGKGALYGALF
HLLNNGLTKGVLFLSSGNIHRSYGNKTTDVVRGALARLPWSGGLFLAGFIAITGSPPFSPFISEYAIVSNAFIDGRHLVG
WLFLLFLVIVFIGMALTVLPMVMGTPPAGLEPSDYEDRLLTVGPPFLMMAVILLLGIWPPAFLVSLLTEGAAMLGVQP

Sequences:

>Translated_478_residues
MLYALVLLPLVGALCAWIVPDNRLRPITLPAVATVHLALTALLLVNTPPPSPGGWIWLDPLGKVVLLCISLLFTVCAYYA
VGYLSYRQERSNRVLCMGLLVCLSAMGLVTMTQHLGLLWVALETTTVSMAPLIYFNRNARSIEATWKYLLLCSVGIGLAL
LGLLFLAYSTYVAHRDATLLLGPLMQSARELNPGWLHAAAIFLLVGYGSKMGLAPLHAWKPDAYGEAPGLVGALLAGGLV
NCAFLCIMRVYQICLASGAELAFFQDALVGMGLISMAFAAVFMAHQADFKRMLAYSSVEHVGILAIGLGLGKGALYGALF
HLLNNGLTKGVLFLSSGNIHRSYGNKTTDVVRGALARLPWSGGLFLAGFIAITGSPPFSPFISEYAIVSNAFIDGRHLVG
WLFLLFLVIVFIGMALTVLPMVMGTPPAGLEPSDYEDRLLTVGPPFLMMAVILLLGIWPPAFLVSLLTEGAAMLGVQP
>Mature_478_residues
MLYALVLLPLVGALCAWIVPDNRLRPITLPAVATVHLALTALLLVNTPPPSPGGWIWLDPLGKVVLLCISLLFTVCAYYA
VGYLSYRQERSNRVLCMGLLVCLSAMGLVTMTQHLGLLWVALETTTVSMAPLIYFNRNARSIEATWKYLLLCSVGIGLAL
LGLLFLAYSTYVAHRDATLLLGPLMQSARELNPGWLHAAAIFLLVGYGSKMGLAPLHAWKPDAYGEAPGLVGALLAGGLV
NCAFLCIMRVYQICLASGAELAFFQDALVGMGLISMAFAAVFMAHQADFKRMLAYSSVEHVGILAIGLGLGKGALYGALF
HLLNNGLTKGVLFLSSGNIHRSYGNKTTDVVRGALARLPWSGGLFLAGFIAITGSPPFSPFISEYAIVSNAFIDGRHLVG
WLFLLFLVIVFIGMALTVLPMVMGTPPAGLEPSDYEDRLLTVGPPFLMMAVILLLGIWPPAFLVSLLTEGAAMLGVQP

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=427, Percent_Identity=34.4262295081967, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1788827, Length=348, Percent_Identity=29.5977011494253, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788829, Length=415, Percent_Identity=27.4698795180723, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788613, Length=237, Percent_Identity=28.2700421940928, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=214, Percent_Identity=29.9065420560748, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367154, Length=352, Percent_Identity=25.5681818181818, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51179; Mature: 51179

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYALVLLPLVGALCAWIVPDNRLRPITLPAVATVHLALTALLLVNTPPPSPGGWIWLDP
CHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHEECCCCCCCCEEEECH
LGKVVLLCISLLFTVCAYYAVGYLSYRQERSNRVLCMGLLVCLSAMGLVTMTQHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEE
ALETTTVSMAPLIYFNRNARSIEATWKYLLLCSVGIGLALLGLLFLAYSTYVAHRDATLL
EEEHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGPLMQSARELNPGWLHAAAIFLLVGYGSKMGLAPLHAWKPDAYGEAPGLVGALLAGGLV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NCAFLCIMRVYQICLASGAELAFFQDALVGMGLISMAFAAVFMAHQADFKRMLAYSSVEH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGILAIGLGLGKGALYGALFHLLNNGLTKGVLFLSSGNIHRSYGNKTTDVVRGALARLPW
HHHHEEECCCCCHHHHHHHHHHHHCCCHHCEEEEECCCEECCCCCCHHHHHHHHHHHCCC
SGGLFLAGFIAITGSPPFSPFISEYAIVSNAFIDGRHLVGWLFLLFLVIVFIGMALTVLP
CCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVMGTPPAGLEPSDYEDRLLTVGPPFLMMAVILLLGIWPPAFLVSLLTEGAAMLGVQP
HHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MLYALVLLPLVGALCAWIVPDNRLRPITLPAVATVHLALTALLLVNTPPPSPGGWIWLDP
CHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHEECCCCCCCCEEEECH
LGKVVLLCISLLFTVCAYYAVGYLSYRQERSNRVLCMGLLVCLSAMGLVTMTQHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEE
ALETTTVSMAPLIYFNRNARSIEATWKYLLLCSVGIGLALLGLLFLAYSTYVAHRDATLL
EEEHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGPLMQSARELNPGWLHAAAIFLLVGYGSKMGLAPLHAWKPDAYGEAPGLVGALLAGGLV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NCAFLCIMRVYQICLASGAELAFFQDALVGMGLISMAFAAVFMAHQADFKRMLAYSSVEH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGILAIGLGLGKGALYGALFHLLNNGLTKGVLFLSSGNIHRSYGNKTTDVVRGALARLPW
HHHHEEECCCCCHHHHHHHHHHHHCCCHHCEEEEECCCEECCCCCCHHHHHHHHHHHCCC
SGGLFLAGFIAITGSPPFSPFISEYAIVSNAFIDGRHLVGWLFLLFLVIVFIGMALTVLP
CCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVMGTPPAGLEPSDYEDRLLTVGPPFLMMAVILLLGIWPPAFLVSLLTEGAAMLGVQP
HHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]