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The map label for this gene is hycD [H]

Identifier: 78223797

GI number: 78223797

Start: 2950044

End: 2950952

Strand: Reverse

Name: hycD [H]

Synonym: Gmet_2600

Alternate gene names: 78223797

Gene position: 2950952-2950044 (Counterclockwise)

Preceding gene: 78223798

Following gene: 78223796

Centisome position: 73.82

GC content: 58.2

Gene sequence:

>909_bases
ATGACAGACATTATCATCCACTGCGCAGTGGCGCTCCTGTTGCCGCCACTGTTGCTCGGGGTTATCGGCAAGACCAAGGC
GGCCTTTGCCGGAAGGGTTGGAGCGCCCTATCTGCAGCCCTATTTCGACCTGATCAAGCTCTTCCGCAAGGGATCGGTCT
TTAGTGAGACCACCACCTGGGTTTTCCGTGCCGGCCCGTTGATAACACTCTCCGCAGCGCTTATCGCAGTGTTGCTGATC
CCGCTGGGCCGCCATGTCGCGCCACTTTCTTTCAGTGGCGATCTCATCCTCTTTGCCTACCTTTTCGGATTGTCCCGGTT
CTTTACCACCATTGCGGCTCTGGATACCGGTTCTAGTTTCGAAGGGATGGGAGCGGCACGTGAAGTTACGTTTTCCTGCC
TTGCCGAACCGACCCTCTTCTTTTCCCTGCTGACTCTGGCCCGCATAAGCAAGTCGCTTTCCCTGTCAACGATACTCACC
CATCCCACGCCCGCCATCTGGCTTGCCGCCGGGGCGAGCATGCTGTTGCTGGTGGGGGCGCTGTTCATCACCCTTCTGGC
GGAGAACTCCCGCATCCCCTTCGACGATCCCAACACGCACCTCGAATTGACCATGATCCATGAAGTCATGGTCCTTGATC
ACAGTGGCCCCGCCTTCGGGATCATCCTCTACGGAGCGGCGCTGAAACTGTTCATTCTCGGAGCGGTCTTTGTAAATATC
GCCTTCCCTGTCGCCACCGGAAATGCGCTTCTCGACTGGGGCATCTTCATTGTCGCCATGCTTGTGCTGGCGGTGGCAAT
CGGCGTTGTCGAGTCGGTGATGGCCCGGTTGCGTCTGGTCCGCGTTCCGCAACTGCTGGTCGGAGCCACTATCCTGTCCG
CCTTTGCCATGCTGCTGGTATTGAGGTAG

Upstream 100 bases:

>100_bases
ATTTACGTGTTGTATATATTCGCCACGCTCTGCGTCCTGATGATCTGGAAGCACTGAACGAGGACAGCGGCGTTCCCGTC
TCCTTCGAGGATGGCACGTT

Downstream 100 bases:

>100_bases
CCCCATGAGTTCATTCGCCGATCAACTGCTGGTACTGGTGATGCTGATCAATCTGATCATGCTTGGCACGAGCAGGCTGA
TGTTTTCCATCCGTGCCGTT

Product: respiratory-chain NADH dehydrogenase subunit 1

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTDIIIHCAVALLLPPLLLGVIGKTKAAFAGRVGAPYLQPYFDLIKLFRKGSVFSETTTWVFRAGPLITLSAALIAVLLI
PLGRHVAPLSFSGDLILFAYLFGLSRFFTTIAALDTGSSFEGMGAAREVTFSCLAEPTLFFSLLTLARISKSLSLSTILT
HPTPAIWLAAGASMLLLVGALFITLLAENSRIPFDDPNTHLELTMIHEVMVLDHSGPAFGIILYGAALKLFILGAVFVNI
AFPVATGNALLDWGIFIVAMLVLAVAIGVVESVMARLRLVRVPQLLVGATILSAFAMLLVLR

Sequences:

>Translated_302_residues
MTDIIIHCAVALLLPPLLLGVIGKTKAAFAGRVGAPYLQPYFDLIKLFRKGSVFSETTTWVFRAGPLITLSAALIAVLLI
PLGRHVAPLSFSGDLILFAYLFGLSRFFTTIAALDTGSSFEGMGAAREVTFSCLAEPTLFFSLLTLARISKSLSLSTILT
HPTPAIWLAAGASMLLLVGALFITLLAENSRIPFDDPNTHLELTMIHEVMVLDHSGPAFGIILYGAALKLFILGAVFVNI
AFPVATGNALLDWGIFIVAMLVLAVAIGVVESVMARLRLVRVPQLLVGATILSAFAMLLVLR
>Mature_301_residues
TDIIIHCAVALLLPPLLLGVIGKTKAAFAGRVGAPYLQPYFDLIKLFRKGSVFSETTTWVFRAGPLITLSAALIAVLLIP
LGRHVAPLSFSGDLILFAYLFGLSRFFTTIAALDTGSSFEGMGAAREVTFSCLAEPTLFFSLLTLARISKSLSLSTILTH
PTPAIWLAAGASMLLLVGALFITLLAENSRIPFDDPNTHLELTMIHEVMVLDHSGPAFGIILYGAALKLFILGAVFVNIA
FPVATGNALLDWGIFIVAMLVLAVAIGVVESVMARLRLVRVPQLLVGATILSAFAMLLVLR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=288, Percent_Identity=32.9861111111111, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082115, Length=244, Percent_Identity=32.3770491803279, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 32264; Mature: 32133

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIIIHCAVALLLPPLLLGVIGKTKAAFAGRVGAPYLQPYFDLIKLFRKGSVFSETTTW
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
VFRAGPLITLSAALIAVLLIPLGRHVAPLSFSGDLILFAYLFGLSRFFTTIAALDTGSSF
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMGAAREVTFSCLAEPTLFFSLLTLARISKSLSLSTILTHPTPAIWLAAGASMLLLVGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LFITLLAENSRIPFDDPNTHLELTMIHEVMVLDHSGPAFGIILYGAALKLFILGAVFVNI
HHHHHHHCCCCCCCCCCCCCEEHEEEHEEHEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AFPVATGNALLDWGIFIVAMLVLAVAIGVVESVMARLRLVRVPQLLVGATILSAFAMLLV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LR
CC
>Mature Secondary Structure 
TDIIIHCAVALLLPPLLLGVIGKTKAAFAGRVGAPYLQPYFDLIKLFRKGSVFSETTTW
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
VFRAGPLITLSAALIAVLLIPLGRHVAPLSFSGDLILFAYLFGLSRFFTTIAALDTGSSF
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMGAAREVTFSCLAEPTLFFSLLTLARISKSLSLSTILTHPTPAIWLAAGASMLLLVGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LFITLLAENSRIPFDDPNTHLELTMIHEVMVLDHSGPAFGIILYGAALKLFILGAVFVNI
HHHHHHHCCCCCCCCCCCCCEEHEEEHEEHEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AFPVATGNALLDWGIFIVAMLVLAVAIGVVESVMARLRLVRVPQLLVGATILSAFAMLLV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]