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The map label for this gene is zraS [H]

Identifier: 78223657

GI number: 78223657

Start: 2777032

End: 2778135

Strand: Reverse

Name: zraS [H]

Synonym: Gmet_2454

Alternate gene names: 78223657

Gene position: 2778135-2777032 (Counterclockwise)

Preceding gene: 78223658

Following gene: 78223656

Centisome position: 69.5

GC content: 63.5

Gene sequence:

>1104_bases
ATGACGCTGACAGGTCCCCTCCGCATCCTGTTTCTTGCCGCAGCAATCATCGGGATCAGCCTCTTTCATTATCTGACTCC
GCTCCATCTTCCTGCGCTCCACGACATCTTCCAGCGGCTCTACTACCTGCCGATCATTCTGGCCGCCCTCTGGTTCGGTC
TGAGGGGGGGGCTCGCCGCTTCCATCATCGTCAGCATCCTTTACGTTCCCCATGTCCTCTTTCAGTGGGGCGTGCATCCG
TCGCTGGAGCTGGAGAAATTCCTGGAGATTCTCCTCTACAACGTGGTGGGGGGAGTTACCGGATTCCTCTGCCAGAAGGA
GGGGGAGCGCCGCGAGGAACTGGAACGCGCGGCCCGGCGCCTCGAAGAATCGTACCGCGCGCTCCGGAGCCAGGCAGACC
TCATTATCGGCATCGAGGAACAACTGCGCCGGGCCGAGCGGCTGTCGGCCCTTGGAGAGCTTTCGGCCATGCTGGCCCAT
GAGATCCGCAATCCCCTCGGCTCTATCCGGGGGACGGCGGAAATCCTTCGGGACGACTTCCAGCCGGGGCATCCGAAGCA
CGAGTTCCTGGAGATTCTCCTCAAGGAGACCGATCGCCTGAACCGGGTGGTCGAGGATTTTCTCCGGCTGTCACGTCCTG
GTGACGGGGAGAGAAAGACCTGCGACCTGGCTGCCGAGCTGCGGGAGTTGGTGACTCTTCTCGGCAGCGATGCCGCGGCC
CGAGGGGTGCGAATTGGCCTTGAGGCTGACGAGGTGCCGCCGGTCACCGGCGACCCGGAGAAACTGCGGCAGGCTTTTCT
CAATTTGATGCTGAACGGCATCCAGGCCACCGGTGCGGGGGGGACTGTTACGGTGAGGGTGAAGATGTTCCCGCGTAGCG
GCGACGACCGCCCCCTTGTGGAGCTGGCGTTTGCCGACACCGGTGCGGGAATCCCGCGGGAGAGCCTCGAAAGGATATTC
ACGCCGTTTTTCACCACGAAGGAGGGGGGGACGGGACTCGGTCTCGCCATCACCCAACGGATCGTGGAGGGACACGGCGG
GACCGTGACGGTGGAGAGCGAGCCGGGGCAGGGGACAACGTTCCGGGTGCGGCTTCCCCTGTAA

Upstream 100 bases:

>100_bases
GGCTCAGTCTGTCTGGTCTATCTCACCCGGTCCGGCGCCGGCCGGGCCTGACCGATTTCTCCCTTGCATTATTGCTGGCC
ATCCTCTACATAATCACACC

Downstream 100 bases:

>100_bases
GGAGTGACGGGTGGCAAATGGCGAGAAGAAAACCGTGCGGGTGCTGATCGTTGACGATGATGTGTCGCTGCGCCGGGTGC
TCGAGTATAATCTCCAGGAG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTLTGPLRILFLAAAIIGISLFHYLTPLHLPALHDIFQRLYYLPIILAALWFGLRGGLAASIIVSILYVPHVLFQWGVHP
SLELEKFLEILLYNVVGGVTGFLCQKEGERREELERAARRLEESYRALRSQADLIIGIEEQLRRAERLSALGELSAMLAH
EIRNPLGSIRGTAEILRDDFQPGHPKHEFLEILLKETDRLNRVVEDFLRLSRPGDGERKTCDLAAELRELVTLLGSDAAA
RGVRIGLEADEVPPVTGDPEKLRQAFLNLMLNGIQATGAGGTVTVRVKMFPRSGDDRPLVELAFADTGAGIPRESLERIF
TPFFTTKEGGTGLGLAITQRIVEGHGGTVTVESEPGQGTTFRVRLPL

Sequences:

>Translated_367_residues
MTLTGPLRILFLAAAIIGISLFHYLTPLHLPALHDIFQRLYYLPIILAALWFGLRGGLAASIIVSILYVPHVLFQWGVHP
SLELEKFLEILLYNVVGGVTGFLCQKEGERREELERAARRLEESYRALRSQADLIIGIEEQLRRAERLSALGELSAMLAH
EIRNPLGSIRGTAEILRDDFQPGHPKHEFLEILLKETDRLNRVVEDFLRLSRPGDGERKTCDLAAELRELVTLLGSDAAA
RGVRIGLEADEVPPVTGDPEKLRQAFLNLMLNGIQATGAGGTVTVRVKMFPRSGDDRPLVELAFADTGAGIPRESLERIF
TPFFTTKEGGTGLGLAITQRIVEGHGGTVTVESEPGQGTTFRVRLPL
>Mature_366_residues
TLTGPLRILFLAAAIIGISLFHYLTPLHLPALHDIFQRLYYLPIILAALWFGLRGGLAASIIVSILYVPHVLFQWGVHPS
LELEKFLEILLYNVVGGVTGFLCQKEGERREELERAARRLEESYRALRSQADLIIGIEEQLRRAERLSALGELSAMLAHE
IRNPLGSIRGTAEILRDDFQPGHPKHEFLEILLKETDRLNRVVEDFLRLSRPGDGERKTCDLAAELRELVTLLGSDAAAR
GVRIGLEADEVPPVTGDPEKLRQAFLNLMLNGIQATGAGGTVTVRVKMFPRSGDDRPLVELAFADTGAGIPRESLERIFT
PFFTTKEGGTGLGLAITQRIVEGHGGTVTVESEPGQGTTFRVRLPL

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=41.5584415584416, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=40, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790300, Length=235, Percent_Identity=32.3404255319149, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789149, Length=284, Percent_Identity=28.8732394366197, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788393, Length=239, Percent_Identity=29.7071129707113, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786783, Length=244, Percent_Identity=30.7377049180328, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994928, Length=219, Percent_Identity=30.1369863013699, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87081816, Length=244, Percent_Identity=33.6065573770492, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=29.5081967213115, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786600, Length=221, Percent_Identity=28.0542986425339, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=25.4980079681275, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=215, Percent_Identity=30.6976744186047, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=28.6324786324786, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786912, Length=285, Percent_Identity=29.4736842105263, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=255, Percent_Identity=28.2352941176471, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789808, Length=222, Percent_Identity=28.8288288288288, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40521; Mature: 40389

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTGPLRILFLAAAIIGISLFHYLTPLHLPALHDIFQRLYYLPIILAALWFGLRGGLAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SIIVSILYVPHVLFQWGVHPSLELEKFLEILLYNVVGGVTGFLCQKEGERREELERAARR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LEESYRALRSQADLIIGIEEQLRRAERLSALGELSAMLAHEIRNPLGSIRGTAEILRDDF
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPGHPKHEFLEILLKETDRLNRVVEDFLRLSRPGDGERKTCDLAAELRELVTLLGSDAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
RGVRIGLEADEVPPVTGDPEKLRQAFLNLMLNGIQATGAGGTVTVRVKMFPRSGDDRPLV
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEE
ELAFADTGAGIPRESLERIFTPFFTTKEGGTGLGLAITQRIVEGHGGTVTVESEPGQGTT
EEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCE
FRVRLPL
EEEEECC
>Mature Secondary Structure 
TLTGPLRILFLAAAIIGISLFHYLTPLHLPALHDIFQRLYYLPIILAALWFGLRGGLAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SIIVSILYVPHVLFQWGVHPSLELEKFLEILLYNVVGGVTGFLCQKEGERREELERAARR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LEESYRALRSQADLIIGIEEQLRRAERLSALGELSAMLAHEIRNPLGSIRGTAEILRDDF
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPGHPKHEFLEILLKETDRLNRVVEDFLRLSRPGDGERKTCDLAAELRELVTLLGSDAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
RGVRIGLEADEVPPVTGDPEKLRQAFLNLMLNGIQATGAGGTVTVRVKMFPRSGDDRPLV
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEE
ELAFADTGAGIPRESLERIFTPFFTTKEGGTGLGLAITQRIVEGHGGTVTVESEPGQGTT
EEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCE
FRVRLPL
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]