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The map label for this gene is ispB [H]

Identifier: 78223658

GI number: 78223658

Start: 2778262

End: 2779230

Strand: Reverse

Name: ispB [H]

Synonym: Gmet_2455

Alternate gene names: 78223658

Gene position: 2779230-2778262 (Counterclockwise)

Preceding gene: 78223662

Following gene: 78223657

Centisome position: 69.53

GC content: 62.64

Gene sequence:

>969_bases
ATGCATTCTGCTCTTGCCCTGGTCGGTGATGACCTGAAAAACGTCGAAGCACAGTTCAAGAAGGACCTCGAGTCGGATGT
CTACCTGATCCGCAAAGTGGGGGAATACGTTCTCGCCAGCGGGGGAAAACGGATCCGGCCGACGCTCCTGCTCCTCGCCG
CCAAGCTGTGCGGCTACACCGGCGACCGGCATGTGCCCCTGGCGAGCGTCATCGAATTCATCCATACCGCCACGCTTCTC
CACGACGACGTGGTGGACAACGCGACCCTGCGCCGCGGCAACGCCTCGGCCAACGAGGTGTGGGGCAACGAGGCATCGGT
CCTCGTGGGCGATTTCCTCTTCTCCAAGTCGTTCTCTCTCATGGTTCAGGCCGGTGACCTGCGGGTTCTCAAGGTCATAT
CCGATGCCACCACCATCATTGCCGAGGGTGAGGTCCTCCAGCTCCTCTGCACGAGCGATCTGGATATGACGCTGGAGCGG
TACATTGAAGTGGTGAAGAGCAAGACCGCCGTCCTCCTCTCCGCTGCCTGCGAGGCGGGAGCCATCCTCGGGAAGGCCAC
GCCGGAGCAGGAACTGGCCCTGCGGGACTTCGGCATGGATCTGGGGATTGCCTTCCAGCTCATGGACGACACCCTCGACT
ACACGGCCAGCGAGGAGCAGTTCGGCAAGGAGATCGGCCATGACCTGGAAGAGGGGAAGATCACCCTCCCGCTCATCCAC
ACCCTCCGCAAGTGCACCGACGAGGAGCGCGAAACCATCGCCGCCGTCGTGGAGAAGGAGGTCATGGAGCCGGGTGACTT
CGAGGCGGTATTCGCCCTGGTCCACAAGTATGGCGGCATCCAGTACACCACCGAGGTGGCAGGCGAGTACATTGCCCGCT
GCAAGGAGCACCTCCAGGCGTTCCCCGCCTCCACGGAGAAGCAGGCCGCGCTCGATCTGGCCGACTACGTGGTGAGCCGC
AACCGTTAA

Upstream 100 bases:

>100_bases
TCAATCCGCGATGGATGGCGGTTTGCGCGGGCAGAAAAGCTTTGACAAAACTGGCGTAATTCCCGTAGTATTGCCCAAAT
CTTAGCCTGAGGTCGTGTTA

Downstream 100 bases:

>100_bases
CGAACCCTCTGCCCCGGTCACACGGCGGCTCAGTCTGTCTGGTCTATCTCACCCGGTCCGGCGCCGGCCGGGCCTGACCG
ATTTCTCCCTTGCATTATTG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MHSALALVGDDLKNVEAQFKKDLESDVYLIRKVGEYVLASGGKRIRPTLLLLAAKLCGYTGDRHVPLASVIEFIHTATLL
HDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSLMVQAGDLRVLKVISDATTIIAEGEVLQLLCTSDLDMTLER
YIEVVKSKTAVLLSAACEAGAILGKATPEQELALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
TLRKCTDEERETIAAVVEKEVMEPGDFEAVFALVHKYGGIQYTTEVAGEYIARCKEHLQAFPASTEKQAALDLADYVVSR
NR

Sequences:

>Translated_322_residues
MHSALALVGDDLKNVEAQFKKDLESDVYLIRKVGEYVLASGGKRIRPTLLLLAAKLCGYTGDRHVPLASVIEFIHTATLL
HDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSLMVQAGDLRVLKVISDATTIIAEGEVLQLLCTSDLDMTLER
YIEVVKSKTAVLLSAACEAGAILGKATPEQELALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
TLRKCTDEERETIAAVVEKEVMEPGDFEAVFALVHKYGGIQYTTEVAGEYIARCKEHLQAFPASTEKQAALDLADYVVSR
NR
>Mature_322_residues
MHSALALVGDDLKNVEAQFKKDLESDVYLIRKVGEYVLASGGKRIRPTLLLLAAKLCGYTGDRHVPLASVIEFIHTATLL
HDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSLMVQAGDLRVLKVISDATTIIAEGEVLQLLCTSDLDMTLER
YIEVVKSKTAVLLSAACEAGAILGKATPEQELALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
TLRKCTDEERETIAAVVEKEVMEPGDFEAVFALVHKYGGIQYTTEVAGEYIARCKEHLQAFPASTEKQAALDLADYVVSR
NR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=319, Percent_Identity=30.0940438871473, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4758430, Length=209, Percent_Identity=24.4019138755981, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI83700220, Length=209, Percent_Identity=24.4019138755981, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=45.9375, Blast_Score=288, Evalue=2e-79,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=28.3842794759825, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17505681, Length=307, Percent_Identity=30.6188925081433, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=30.5647840531561, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=245, Percent_Identity=25.7142857142857, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=29.5302013422819, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281365769, Length=257, Percent_Identity=22.1789883268482, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24660002, Length=257, Percent_Identity=22.1789883268482, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35283; Mature: 35283

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSALALVGDDLKNVEAQFKKDLESDVYLIRKVGEYVLASGGKRIRPTLLLLAAKLCGYT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GDRHVPLASVIEFIHTATLLHDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE
MVQAGDLRVLKVISDATTIIAEGEVLQLLCTSDLDMTLERYIEVVKSKTAVLLSAACEAG
EEEECCEEEEHHHHHHHHEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
AILGKATPEQELALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
HHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCEEHHHHH
TLRKCTDEERETIAAVVEKEVMEPGDFEAVFALVHKYGGIQYTTEVAGEYIARCKEHLQA
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
FPASTEKQAALDLADYVVSRNR
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHSALALVGDDLKNVEAQFKKDLESDVYLIRKVGEYVLASGGKRIRPTLLLLAAKLCGYT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GDRHVPLASVIEFIHTATLLHDDVVDNATLRRGNASANEVWGNEASVLVGDFLFSKSFSL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE
MVQAGDLRVLKVISDATTIIAEGEVLQLLCTSDLDMTLERYIEVVKSKTAVLLSAACEAG
EEEECCEEEEHHHHHHHHEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
AILGKATPEQELALRDFGMDLGIAFQLMDDTLDYTASEEQFGKEIGHDLEEGKITLPLIH
HHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCEEHHHHH
TLRKCTDEERETIAAVVEKEVMEPGDFEAVFALVHKYGGIQYTTEVAGEYIARCKEHLQA
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
FPASTEKQAALDLADYVVSRNR
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]