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The map label for this gene is pncA [H]

Identifier: 78223577

GI number: 78223577

Start: 2683974

End: 2684573

Strand: Direct

Name: pncA [H]

Synonym: Gmet_2374

Alternate gene names: 78223577

Gene position: 2683974-2684573 (Clockwise)

Preceding gene: 78223576

Following gene: 78223578

Centisome position: 67.14

GC content: 61.83

Gene sequence:

>600_bases
ATGAAGAGCACGTCAGCCCTTCTGATCGTCGACGTCCAGAACGATTTCTGCCCCGGAGGCTTGCTACCCGTACCAGAGGG
GGATCGGGTCGTCCCGCTGCTCAACCGCTACATGGAGCTTTTCAGAGAGAAAAAGCTGCCGATCATCGCATCCCGGGACT
GGCATCCGGCCATCACATCCCACTTTCGGGACTTCGGTGGCATCTGGCCGGTCCACTGCGTCCAGGGGAGCGAGGGGGCC
CGGTTCCACCGCGACCTGGCCCTTCCGGACGACGCCATTGTGATCTCCAAGGGACAAGACCCGGCTCAGGACGCCTATTC
CGCGTTCCAGGCGACCACGGAAAGCGGCGTGTCCTTCCCGGAACTCCTTAAAGAGCTGGGTATCACCAGACTTTTCGTGG
GGGGCCTCGCCACGGACTACTGTGTCAAGGAATCGGTTCTGGACGGGCTGCGCCACGGTCTTGCGGTAACACTCCTTGAG
GATGCGGTACGGGGGGTCAACCTGACTCCCGGCGACTCGGCAAGGGCTATCGAAAACATGGTCGTTGCCGGCGCGGTGCG
AATGAATTTCAGCAGGCTTCAGGCCGACCCTCCCTCGTGA

Upstream 100 bases:

>100_bases
AATACAAGGTCTCCATCAGCGCCGGTCTCAACGAGCTACGGCTGCAGCTTATGGACGAGGTTCAACGGGGATATAAATAG
ATCCAGCAGGAGAACTATCC

Downstream 100 bases:

>100_bases
CCGTCCATGAGAGGGAATACATCCCGTTTGACTTTTGAGGGGGAAAGCGCTAGAGTGATTCGTCTCAAATCCCCATGAAA
TCAAAGGACAATTCCCGTAA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKSTSALLIVDVQNDFCPGGLLPVPEGDRVVPLLNRYMELFREKKLPIIASRDWHPAITSHFRDFGGIWPVHCVQGSEGA
RFHRDLALPDDAIVISKGQDPAQDAYSAFQATTESGVSFPELLKELGITRLFVGGLATDYCVKESVLDGLRHGLAVTLLE
DAVRGVNLTPGDSARAIENMVVAGAVRMNFSRLQADPPS

Sequences:

>Translated_199_residues
MKSTSALLIVDVQNDFCPGGLLPVPEGDRVVPLLNRYMELFREKKLPIIASRDWHPAITSHFRDFGGIWPVHCVQGSEGA
RFHRDLALPDDAIVISKGQDPAQDAYSAFQATTESGVSFPELLKELGITRLFVGGLATDYCVKESVLDGLRHGLAVTLLE
DAVRGVNLTPGDSARAIENMVVAGAVRMNFSRLQADPPS
>Mature_199_residues
MKSTSALLIVDVQNDFCPGGLLPVPEGDRVVPLLNRYMELFREKKLPIIASRDWHPAITSHFRDFGGIWPVHCVQGSEGA
RFHRDLALPDDAIVISKGQDPAQDAYSAFQATTESGVSFPELLKELGITRLFVGGLATDYCVKESVLDGLRHGLAVTLLE
DAVRGVNLTPGDSARAIENMVVAGAVRMNFSRLQADPPS

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=197, Percent_Identity=44.1624365482233, Blast_Score=154, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI193206735, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543130, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648247, Length=206, Percent_Identity=30.5825242718447, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21626; Mature: 21626

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTSALLIVDVQNDFCPGGLLPVPEGDRVVPLLNRYMELFREKKLPIIASRDWHPAITS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHH
HFRDFGGIWPVHCVQGSEGARFHRDLALPDDAIVISKGQDPAQDAYSAFQATTESGVSFP
HHHHHCCCCCEEEECCCCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHH
ELLKELGITRLFVGGLATDYCVKESVLDGLRHGLAVTLLEDAVRGVNLTPGDSARAIENM
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VVAGAVRMNFSRLQADPPS
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSTSALLIVDVQNDFCPGGLLPVPEGDRVVPLLNRYMELFREKKLPIIASRDWHPAITS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHH
HFRDFGGIWPVHCVQGSEGARFHRDLALPDDAIVISKGQDPAQDAYSAFQATTESGVSFP
HHHHHCCCCCEEEECCCCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHH
ELLKELGITRLFVGGLATDYCVKESVLDGLRHGLAVTLLEDAVRGVNLTPGDSARAIENM
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VVAGAVRMNFSRLQADPPS
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]