Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 78223231

GI number: 78223231

Start: 2263892

End: 2265862

Strand: Reverse

Name: asnB [H]

Synonym: Gmet_2024

Alternate gene names: 78223231

Gene position: 2265862-2263892 (Counterclockwise)

Preceding gene: 78223232

Following gene: 78223230

Centisome position: 56.68

GC content: 62.66

Gene sequence:

>1971_bases
ATGAGCCAGGGAATTTTCGGGATAATCGATTTCAGGAACGCCATTGCCGACAGGGATGTGCTGGGTAATGCCATGGCGGC
CTACCTGAAGATGAGTGGCAGTGGTGAGTTCTCCGTCTCCACGGGGCTCGGCCATCTCATCGGCATGCGGCGGAGCGCCG
CCGACTCCCCGAGCCGCCAGACCCGCATAGAGCACGATCCCGACGCCGGTCTTGCGGCGGTTATCCACGGCGAGGTCAAC
AACTACCGGGAGCTCCTCTCGCAGCATCCACCCTCGGGGCGTCCCTGCTGCGGCGACCTTGACCTCGTCCTGCGCATGTA
CCGGCGCAGCGGCGCCTCCTTCGCCCGCTCCATCAACGGCCTCTTCACCATCGGCATCCTCGACGAACGGGAAAAGACCT
TCCGCCTCATGAACGACCGCTTCGGCCTCGCCCACCAGCTCTACTGGACCGTGGCCGACGGACGGCTTATCTTCGCCACC
CATCTCAAAACCCTCCTGGCCTGTCCGGGGGTAAGGCGGGAACTGGACCACGAAGGCCTCAACCTGTTCCTGAAATACGC
CTACATCACCTCTCCCTGGTCGATCCTCAAAGGGATCAACAAGCTGCCGCCGGGGCACATGCTCACTTTCCGTGATGGCA
AAGCGGAGGTTGCCCCCTACTGGCAGTTTGATGCCGTGACCCGCCCAGGCATGACCATGGAAGAAGCGATTCCCGCCTAC
CGTGATCTACTGCGCGGTGCAATCGCCAGGCGCATGGGGAGCGACGGCAGCACCGGCGTCATGTTGAGCGGCGGGCTCGA
TTCCAGCGCCAACGCGGCCCTGGCTGCCTCCTGCTCCAGTACGCCGGTCAAGACCTTCGCCATCGGCTTTGAAGACCCCC
GTTTCGACGAGCGCCCCTTCGCCCGGATCGTGGCAAAGCACTTCGGCACCGATCACCATGAGTACACCATCACCGGTCGC
GAGATCGACGATCTCCCTTCCCTGGTGTGGCACCTGGAAGAGCCCTATTTCGAGTTCGGCCTCTTCCTCACCTACATGGG
GCTCAAATCGGCCAGCGGCCACGTCCAGGCGGTCATCGGCGGCGAAGGGGCCGACCAGATGTACGGCACCGGCGGTTTCG
CCGGCGGCCGACCTGCTGCCGTCCACTACCTGTTCCGCAAGAGCGGCATGATCGGTCCGGCCCGCACCGTCTCAAGCTTG
CTCCGGGGTCCCCTGTTCTATGATCGCGACAACCTCGGCTTCCAGTTGCGGCTCTTCTGGAATCGGGCAGCAGATCTGAA
CGACTGGTACTTCTACGGTTACGACGGCCACGAACTGAGCCAACTTTACAGAAACGGCAACCTGGCCCGGGTGCCGCGTA
TCTTCGGCGAGCCCGCGGGCAGCCCGGAATCCTTTGCCCAGGTTTACCTGGAAACCCAGATCGAGCAGGACATGCGGCAC
TACGTCAACGAGAATGTCATGGTCAAGTCCGGCCGCATGGCCGACATGCTTGGCCTGGTGCTCCGTGAGTCGTTCCTGGA
CGCCGATGTGGCCGACTTCCTGGTGTCTCTCCCCATGCCCGTCAAACGGGGCGGGGGGCTTGTGGACCACCTCCGGGGGA
GATTCAAGAGCAAGCTCCTGCACCGCAAGGCCATGGAGGGGATCCTCCCCCCGGAAATCATGTCCAAGCCCAAGCAGGGG
GGATTCGTGCCGGTCATGATCTTCCTCAACGACCCGGCCCTGAGGAAACGGATCTACGAGAAGCTGCTCCGGTCCGAAGT
CATGGGAGAGTATTTCCGCACCGATTATCTCCGTACCCTGTTCGAGAAATACGAGTCGTTCCAGCAGGGGGCGGTCGGCT
GGCCCAACTTCCTCAACTCGAAGGCGAACCGGATCCTGTTCCTGCTCACCTTCGACATCTGGCATCACTGCTACCTGGCC
AACGATCCCCTCGGCGTCACGCCGCCGAGCCTGAACGAGTATCTGGGGTAG

Upstream 100 bases:

>100_bases
GAGGGGTCCTATTTTTCCTTTCCGACCAAAGACCACGTGCGGGAATTCAAACAGAACGGCAAGAGGCTGATCTGAGCGCG
GCCATGAAGGATCATCACCA

Downstream 100 bases:

>100_bases
GGCGACGATGGACACGATTCCGGCAATGACAGCCCCGGTCCTCATGTACCATGAGGTAACCGCCACCAGCGAGCAGGACA
AGGCGGTGCGAAAGACCAAC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MSQGIFGIIDFRNAIADRDVLGNAMAAYLKMSGSGEFSVSTGLGHLIGMRRSAADSPSRQTRIEHDPDAGLAAVIHGEVN
NYRELLSQHPPSGRPCCGDLDLVLRMYRRSGASFARSINGLFTIGILDEREKTFRLMNDRFGLAHQLYWTVADGRLIFAT
HLKTLLACPGVRRELDHEGLNLFLKYAYITSPWSILKGINKLPPGHMLTFRDGKAEVAPYWQFDAVTRPGMTMEEAIPAY
RDLLRGAIARRMGSDGSTGVMLSGGLDSSANAALAASCSSTPVKTFAIGFEDPRFDERPFARIVAKHFGTDHHEYTITGR
EIDDLPSLVWHLEEPYFEFGLFLTYMGLKSASGHVQAVIGGEGADQMYGTGGFAGGRPAAVHYLFRKSGMIGPARTVSSL
LRGPLFYDRDNLGFQLRLFWNRAADLNDWYFYGYDGHELSQLYRNGNLARVPRIFGEPAGSPESFAQVYLETQIEQDMRH
YVNENVMVKSGRMADMLGLVLRESFLDADVADFLVSLPMPVKRGGGLVDHLRGRFKSKLLHRKAMEGILPPEIMSKPKQG
GFVPVMIFLNDPALRKRIYEKLLRSEVMGEYFRTDYLRTLFEKYESFQQGAVGWPNFLNSKANRILFLLTFDIWHHCYLA
NDPLGVTPPSLNEYLG

Sequences:

>Translated_656_residues
MSQGIFGIIDFRNAIADRDVLGNAMAAYLKMSGSGEFSVSTGLGHLIGMRRSAADSPSRQTRIEHDPDAGLAAVIHGEVN
NYRELLSQHPPSGRPCCGDLDLVLRMYRRSGASFARSINGLFTIGILDEREKTFRLMNDRFGLAHQLYWTVADGRLIFAT
HLKTLLACPGVRRELDHEGLNLFLKYAYITSPWSILKGINKLPPGHMLTFRDGKAEVAPYWQFDAVTRPGMTMEEAIPAY
RDLLRGAIARRMGSDGSTGVMLSGGLDSSANAALAASCSSTPVKTFAIGFEDPRFDERPFARIVAKHFGTDHHEYTITGR
EIDDLPSLVWHLEEPYFEFGLFLTYMGLKSASGHVQAVIGGEGADQMYGTGGFAGGRPAAVHYLFRKSGMIGPARTVSSL
LRGPLFYDRDNLGFQLRLFWNRAADLNDWYFYGYDGHELSQLYRNGNLARVPRIFGEPAGSPESFAQVYLETQIEQDMRH
YVNENVMVKSGRMADMLGLVLRESFLDADVADFLVSLPMPVKRGGGLVDHLRGRFKSKLLHRKAMEGILPPEIMSKPKQG
GFVPVMIFLNDPALRKRIYEKLLRSEVMGEYFRTDYLRTLFEKYESFQQGAVGWPNFLNSKANRILFLLTFDIWHHCYLA
NDPLGVTPPSLNEYLG
>Mature_655_residues
SQGIFGIIDFRNAIADRDVLGNAMAAYLKMSGSGEFSVSTGLGHLIGMRRSAADSPSRQTRIEHDPDAGLAAVIHGEVNN
YRELLSQHPPSGRPCCGDLDLVLRMYRRSGASFARSINGLFTIGILDEREKTFRLMNDRFGLAHQLYWTVADGRLIFATH
LKTLLACPGVRRELDHEGLNLFLKYAYITSPWSILKGINKLPPGHMLTFRDGKAEVAPYWQFDAVTRPGMTMEEAIPAYR
DLLRGAIARRMGSDGSTGVMLSGGLDSSANAALAASCSSTPVKTFAIGFEDPRFDERPFARIVAKHFGTDHHEYTITGRE
IDDLPSLVWHLEEPYFEFGLFLTYMGLKSASGHVQAVIGGEGADQMYGTGGFAGGRPAAVHYLFRKSGMIGPARTVSSLL
RGPLFYDRDNLGFQLRLFWNRAADLNDWYFYGYDGHELSQLYRNGNLARVPRIFGEPAGSPESFAQVYLETQIEQDMRHY
VNENVMVKSGRMADMLGLVLRESFLDADVADFLVSLPMPVKRGGGLVDHLRGRFKSKLLHRKAMEGILPPEIMSKPKQGG
FVPVMIFLNDPALRKRIYEKLLRSEVMGEYFRTDYLRTLFEKYESFQQGAVGWPNFLNSKANRILFLLTFDIWHHCYLAN
DPLGVTPPSLNEYLG

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010848, Length=308, Percent_Identity=25.6493506493506, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI168229248, Length=308, Percent_Identity=25.6493506493506, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI168229252, Length=308, Percent_Identity=25.6493506493506, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI168229250, Length=308, Percent_Identity=25.6493506493506, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI296010852, Length=285, Percent_Identity=25.9649122807018, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI296010850, Length=285, Percent_Identity=25.9649122807018, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786889, Length=318, Percent_Identity=23.2704402515723, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71993933, Length=315, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25147557, Length=315, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73403; Mature: 73272

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGIFGIIDFRNAIADRDVLGNAMAAYLKMSGSGEFSVSTGLGHLIGMRRSAADSPSRQ
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHHHHHHHHHCCCCCCCC
TRIEHDPDAGLAAVIHGEVNNYRELLSQHPPSGRPCCGDLDLVLRMYRRSGASFARSING
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCC
LFTIGILDEREKTFRLMNDRFGLAHQLYWTVADGRLIFATHLKTLLACPGVRRELDHEGL
EEEEEEECCHHHHHHHHHHHCCCEEEEEEEEECCCEEHHHHHHHHHHCCCHHHHHCCCCC
NLFLKYAYITSPWSILKGINKLPPGHMLTFRDGKAEVAPYWQFDAVTRPGMTMEEAIPAY
EEEEEEHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHH
RDLLRGAIARRMGSDGSTGVMLSGGLDSSANAALAASCSSTPVKTFAIGFEDPRFDERPF
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCHH
ARIVAKHFGTDHHEYTITGREIDDLPSLVWHLEEPYFEFGLFLTYMGLKSASGHVQAVIG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEC
GEGADQMYGTGGFAGGRPAAVHYLFRKSGMIGPARTVSSLLRGPLFYDRDNLGFQLRLFW
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEECCCCCCEEEEEEE
NRAADLNDWYFYGYDGHELSQLYRNGNLARVPRIFGEPAGSPESFAQVYLETQIEQDMRH
CCCCCCCCCEEECCCHHHHHHHHHCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
YVNENVMVKSGRMADMLGLVLRESFLDADVADFLVSLPMPVKRGGGLVDHLRGRFKSKLL
HCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
HRKAMEGILPPEIMSKPKQGGFVPVMIFLNDPALRKRIYEKLLRSEVMGEYFRTDYLRTL
HHHHHHCCCCHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKYESFQQGAVGWPNFLNSKANRILFLLTFDIWHHCYLANDPLGVTPPSLNEYLG
HHHHHHHHCCCCCCCHHHCCCCCEEEEEEEEHHHHHEEECCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SQGIFGIIDFRNAIADRDVLGNAMAAYLKMSGSGEFSVSTGLGHLIGMRRSAADSPSRQ
CCCCEEEEEHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHHHHHHHHHCCCCCCCC
TRIEHDPDAGLAAVIHGEVNNYRELLSQHPPSGRPCCGDLDLVLRMYRRSGASFARSING
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCC
LFTIGILDEREKTFRLMNDRFGLAHQLYWTVADGRLIFATHLKTLLACPGVRRELDHEGL
EEEEEEECCHHHHHHHHHHHCCCEEEEEEEEECCCEEHHHHHHHHHHCCCHHHHHCCCCC
NLFLKYAYITSPWSILKGINKLPPGHMLTFRDGKAEVAPYWQFDAVTRPGMTMEEAIPAY
EEEEEEHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHH
RDLLRGAIARRMGSDGSTGVMLSGGLDSSANAALAASCSSTPVKTFAIGFEDPRFDERPF
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCHH
ARIVAKHFGTDHHEYTITGREIDDLPSLVWHLEEPYFEFGLFLTYMGLKSASGHVQAVIG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEC
GEGADQMYGTGGFAGGRPAAVHYLFRKSGMIGPARTVSSLLRGPLFYDRDNLGFQLRLFW
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEECCCCCCEEEEEEE
NRAADLNDWYFYGYDGHELSQLYRNGNLARVPRIFGEPAGSPESFAQVYLETQIEQDMRH
CCCCCCCCCEEECCCHHHHHHHHHCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
YVNENVMVKSGRMADMLGLVLRESFLDADVADFLVSLPMPVKRGGGLVDHLRGRFKSKLL
HCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
HRKAMEGILPPEIMSKPKQGGFVPVMIFLNDPALRKRIYEKLLRSEVMGEYFRTDYLRTL
HHHHHHCCCCHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKYESFQQGAVGWPNFLNSKANRILFLLTFDIWHHCYLANDPLGVTPPSLNEYLG
HHHHHHHHCCCCCCCHHHCCCCCEEEEEEEEHHHHHEEECCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]