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The map label for this gene is rpfG [H]

Identifier: 78223198

GI number: 78223198

Start: 2220896

End: 2223163

Strand: Direct

Name: rpfG [H]

Synonym: Gmet_1991

Alternate gene names: 78223198

Gene position: 2220896-2223163 (Clockwise)

Preceding gene: 78223197

Following gene: 78223202

Centisome position: 55.56

GC content: 55.11

Gene sequence:

>2268_bases
ATGACCTCCGTACCAGACGACCTCCGGATGTTCGAAAAGAAGTTCTCCATACTACAGGAAATATCGAATGCCTTGGTGGT
TACGGACAACCTGAGTGCAATCGCGAACCTCATGCTCGACCTGGCCATCAACTACACAAGGGCCGAGAAGGGGTCTCTCA
TGCTCGTCAACGAGCGGGATGAACTCTACATTCTTGCGGGACGCGGCATAGATCTCAACCTTGTGCGAACCTACCGGGCA
AAGATCGGCGAAGGGATTGCCGGGGTTGTGGCAAAGAACCGCACTCCCGTGCTTGTGGAAGATCTGGCCAACGAGCCGCG
GTTCAAGGGGATAACCCGCGACCGCTACCGGACCAACTCCTTCGCATCATGCCCCATCATAAGCAAGAACCGGCTCCTTG
GTGTCCTCAACATCAACGACAAGGTTGACGGAGCCCCCTTCAACGAAGACGAGTTCTCCCTCATCAAAATCATTGCCAAT
CAGGCAGCTATTGCCATGGAGAATGCCATTCTCGTCGGACAACTCCGATTCAAAGCCGCAGAACAGGAAGGGATCAACCG
CAAGCTCATCGAAAGTGACGTGGTAAAGACCGAATTCATCACCCGGGTATCCCACGAATTGCGTACCCCCCTCAACTCCA
TCAAGGGTTCCGTTTACTACCTGAGGCAGTCCGAACGCCTCTCGCGGCGCGAACAGGAAGAGTTTTACGACATCATCTCC
GGCGAGACGGACAAACTGATCGGCATCGTGGAAAACCAGCTTGACTTCCTCCGCTTTGAAGACGAGACGATGCTCATAAA
CAAGACCCTGGTAAGCCTCAGGGAGGTACTGAATGAAGTGTGCGGCTCAAAGAACATCAAAACCCTCTTTGCCCGCAAAG
ATATAAGCATTGATGTGGAGATAGGTGATGGACTTTCAGATGTAGTCGGCGACAGAATCAGGATAGTTCAGTTTTTCCTC
AACATTCTTGAAGGGTTGAGCTACTTTCTGGAGCGGGGCGACGTGGTTCGCATTTCCGCCCGGGAGAACGATGACATTCA
CGTATCCATAGGACTTCCGCGCAGACTGCCCGAGAGCATCACCCCTTACCTTTTCAGTTCGAATCATCTGTTTCAGACCG
ATTCCCCCGACGAAAAACTCAAACTCTACCTGGCCCGGAAGGTAGTGGACATTCATCGCTGGGATCTGGTCGCGGAAAAC
ACCGCCACGGGTCTCCTAGTTTCGCTTATCATACCAAAGAGCGCCCGCCAGAAGGTAGACGCCGTCGTCAATACAACCCT
CGATATGTTCCTGGAGTTCATTGCCGAGGTACTCGATGTGAACATGTGCTCGGTGATGCTCTGCGACGACATGACGGGCG
ATCTCATGATCAAGGGCGCGAGAGGCCTCGACGACCACATGATCAAGCAGACCCGCGTGCGGATGGGAGACCGGATCGCC
GGGTGGGTCGCCCTTGAGGGTGAACCGCTCTTTATCGAGGATATCGAGCAGGATCCCCGTTTTGGGAGAAAGAGCGTTCC
CCAGTACAACAGCAAATCGCTCATTTCGCTCCCCCTCAAGGTGGGCGGCAAGGTCATCGGCGTCCTGAACCTCAACAACA
AGCGCTCAGCCAGTCCTTTCACCGGGCGGGACTTCGATCTGGCCCAGGCGATGTGCGAACGGGTTGCCTACTTCATGGGC
CGGGTCCACGGGGGGGATCACCAGGGGGACGAATTTCGGCAGCTGATGACCTCGTTCGACAGCCTCATCACTGCCGAAAA
GAAATACCACAAGAAGGATCCCCGCCTCCCCGACATTGCCATGGGCATCGCTGCTCAAATCGGAATCGGAGAAGATGAGC
GAAGGTCAATACTCTTTGCGTCGGTTCTCTACGACCTGGGGCTTGTGCTCCTCGAAGAGAGCATCCTGAACAAGAAGGGG
AAACTCCTCCCCTCGGAAGAAAACACCCTCAAGATTCACCCTTACACAACGGTGGGGCTCCTGGATTCATTCGAATTCTC
CGAAGAGATCAAGCGGGGGATTCTCCACCACCATGAACGGTATGACGGGACCGGTTATCCCGACGGACTGAAGGGAGACG
AGATTCCGCTCATCTCGCGGATCCTGTCGGTTGTGGACGCCTATTGCGCCATGACGTCGGAAAGGCCGTACCGTCCCCGA
ATGGACATGGCCGATGCCCTTGCAGAAGTTCACAAGCATACGGGGACGCTCTACGACCCGAAGGTGGTGAAGGCGTTGGA
TTTCGTCGTTGGCGAGGGGGTGGAGTAG

Upstream 100 bases:

>100_bases
ACACTAAATTCTGGCACTCTTGTTGCGTACTATTACGTGACCCCGTTCTTCTTTCAGCGATGTTGAAAAAAGATACCCCA
TTGCGGGAGTAGGCGTTCAC

Downstream 100 bases:

>100_bases
CAAGACCAGCGGACGGCTGTTCCGCCGTGCCAGTCACCAATCATGCGTTAATGCCGTGCTTCCTCATGAGATCATAGAGT
GTTGGCCTGCTGATTCCCAA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTSVPDDLRMFEKKFSILQEISNALVVTDNLSAIANLMLDLAINYTRAEKGSLMLVNERDELYILAGRGIDLNLVRTYRA
KIGEGIAGVVAKNRTPVLVEDLANEPRFKGITRDRYRTNSFASCPIISKNRLLGVLNINDKVDGAPFNEDEFSLIKIIAN
QAAIAMENAILVGQLRFKAAEQEGINRKLIESDVVKTEFITRVSHELRTPLNSIKGSVYYLRQSERLSRREQEEFYDIIS
GETDKLIGIVENQLDFLRFEDETMLINKTLVSLREVLNEVCGSKNIKTLFARKDISIDVEIGDGLSDVVGDRIRIVQFFL
NILEGLSYFLERGDVVRISARENDDIHVSIGLPRRLPESITPYLFSSNHLFQTDSPDEKLKLYLARKVVDIHRWDLVAEN
TATGLLVSLIIPKSARQKVDAVVNTTLDMFLEFIAEVLDVNMCSVMLCDDMTGDLMIKGARGLDDHMIKQTRVRMGDRIA
GWVALEGEPLFIEDIEQDPRFGRKSVPQYNSKSLISLPLKVGGKVIGVLNLNNKRSASPFTGRDFDLAQAMCERVAYFMG
RVHGGDHQGDEFRQLMTSFDSLITAEKKYHKKDPRLPDIAMGIAAQIGIGEDERRSILFASVLYDLGLVLLEESILNKKG
KLLPSEENTLKIHPYTTVGLLDSFEFSEEIKRGILHHHERYDGTGYPDGLKGDEIPLISRILSVVDAYCAMTSERPYRPR
MDMADALAEVHKHTGTLYDPKVVKALDFVVGEGVE

Sequences:

>Translated_755_residues
MTSVPDDLRMFEKKFSILQEISNALVVTDNLSAIANLMLDLAINYTRAEKGSLMLVNERDELYILAGRGIDLNLVRTYRA
KIGEGIAGVVAKNRTPVLVEDLANEPRFKGITRDRYRTNSFASCPIISKNRLLGVLNINDKVDGAPFNEDEFSLIKIIAN
QAAIAMENAILVGQLRFKAAEQEGINRKLIESDVVKTEFITRVSHELRTPLNSIKGSVYYLRQSERLSRREQEEFYDIIS
GETDKLIGIVENQLDFLRFEDETMLINKTLVSLREVLNEVCGSKNIKTLFARKDISIDVEIGDGLSDVVGDRIRIVQFFL
NILEGLSYFLERGDVVRISARENDDIHVSIGLPRRLPESITPYLFSSNHLFQTDSPDEKLKLYLARKVVDIHRWDLVAEN
TATGLLVSLIIPKSARQKVDAVVNTTLDMFLEFIAEVLDVNMCSVMLCDDMTGDLMIKGARGLDDHMIKQTRVRMGDRIA
GWVALEGEPLFIEDIEQDPRFGRKSVPQYNSKSLISLPLKVGGKVIGVLNLNNKRSASPFTGRDFDLAQAMCERVAYFMG
RVHGGDHQGDEFRQLMTSFDSLITAEKKYHKKDPRLPDIAMGIAAQIGIGEDERRSILFASVLYDLGLVLLEESILNKKG
KLLPSEENTLKIHPYTTVGLLDSFEFSEEIKRGILHHHERYDGTGYPDGLKGDEIPLISRILSVVDAYCAMTSERPYRPR
MDMADALAEVHKHTGTLYDPKVVKALDFVVGEGVE
>Mature_754_residues
TSVPDDLRMFEKKFSILQEISNALVVTDNLSAIANLMLDLAINYTRAEKGSLMLVNERDELYILAGRGIDLNLVRTYRAK
IGEGIAGVVAKNRTPVLVEDLANEPRFKGITRDRYRTNSFASCPIISKNRLLGVLNINDKVDGAPFNEDEFSLIKIIANQ
AAIAMENAILVGQLRFKAAEQEGINRKLIESDVVKTEFITRVSHELRTPLNSIKGSVYYLRQSERLSRREQEEFYDIISG
ETDKLIGIVENQLDFLRFEDETMLINKTLVSLREVLNEVCGSKNIKTLFARKDISIDVEIGDGLSDVVGDRIRIVQFFLN
ILEGLSYFLERGDVVRISARENDDIHVSIGLPRRLPESITPYLFSSNHLFQTDSPDEKLKLYLARKVVDIHRWDLVAENT
ATGLLVSLIIPKSARQKVDAVVNTTLDMFLEFIAEVLDVNMCSVMLCDDMTGDLMIKGARGLDDHMIKQTRVRMGDRIAG
WVALEGEPLFIEDIEQDPRFGRKSVPQYNSKSLISLPLKVGGKVIGVLNLNNKRSASPFTGRDFDLAQAMCERVAYFMGR
VHGGDHQGDEFRQLMTSFDSLITAEKKYHKKDPRLPDIAMGIAAQIGIGEDERRSILFASVLYDLGLVLLEESILNKKGK
LLPSEENTLKIHPYTTVGLLDSFEFSEEIKRGILHHHERYDGTGYPDGLKGDEIPLISRILSVVDAYCAMTSERPYRPRM
DMADALAEVHKHTGTLYDPKVVKALDFVVGEGVE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=197, Percent_Identity=26.3959390862944, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 84936; Mature: 84805

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVPDDLRMFEKKFSILQEISNALVVTDNLSAIANLMLDLAINYTRAEKGSLMLVNERD
CCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCEEECCCCCEEEEECCC
ELYILAGRGIDLNLVRTYRAKIGEGIAGVVAKNRTPVLVEDLANEPRFKGITRDRYRTNS
CEEEEECCCCEEHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
FASCPIISKNRLLGVLNINDKVDGAPFNEDEFSLIKIIANQAAIAMENAILVGQLRFKAA
CCCCCEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEHHHHHHH
EQEGINRKLIESDVVKTEFITRVSHELRTPLNSIKGSVYYLRQSERLSRREQEEFYDIIS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECHHHHHHHHHHHHHHHHC
GETDKLIGIVENQLDFLRFEDETMLINKTLVSLREVLNEVCGSKNIKTLFARKDISIDVE
CCCHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEE
IGDGLSDVVGDRIRIVQFFLNILEGLSYFLERGDVVRISARENDDIHVSIGLPRRLPESI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHCCHHC
TPYLFSSNHLFQTDSPDEKLKLYLARKVVDIHRWDLVAENTATGLLVSLIIPKSARQKVD
CCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCHHHHHHH
AVVNTTLDMFLEFIAEVLDVNMCSVMLCDDMTGDLMIKGARGLDDHMIKQTRVRMGDRIA
HHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCCEE
GWVALEGEPLFIEDIEQDPRFGRKSVPQYNSKSLISLPLKVGGKVIGVLNLNNKRSASPF
EEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHCCCCEEEEEEECCCCCCCCCC
TGRDFDLAQAMCERVAYFMGRVHGGDHQGDEFRQLMTSFDSLITAEKKYHKKDPRLPDIA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
MGIAAQIGIGEDERRSILFASVLYDLGLVLLEESILNKKGKLLPSEENTLKIHPYTTVGL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEHH
LDSFEFSEEIKRGILHHHERYDGTGYPDGLKGDEIPLISRILSVVDAYCAMTSERPYRPR
HHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
MDMADALAEVHKHTGTLYDPKVVKALDFVVGEGVE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSVPDDLRMFEKKFSILQEISNALVVTDNLSAIANLMLDLAINYTRAEKGSLMLVNERD
CCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCEEECCCCCEEEEECCC
ELYILAGRGIDLNLVRTYRAKIGEGIAGVVAKNRTPVLVEDLANEPRFKGITRDRYRTNS
CEEEEECCCCEEHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
FASCPIISKNRLLGVLNINDKVDGAPFNEDEFSLIKIIANQAAIAMENAILVGQLRFKAA
CCCCCEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEHHHHHHH
EQEGINRKLIESDVVKTEFITRVSHELRTPLNSIKGSVYYLRQSERLSRREQEEFYDIIS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECHHHHHHHHHHHHHHHHC
GETDKLIGIVENQLDFLRFEDETMLINKTLVSLREVLNEVCGSKNIKTLFARKDISIDVE
CCCHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEE
IGDGLSDVVGDRIRIVQFFLNILEGLSYFLERGDVVRISARENDDIHVSIGLPRRLPESI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHCCHHC
TPYLFSSNHLFQTDSPDEKLKLYLARKVVDIHRWDLVAENTATGLLVSLIIPKSARQKVD
CCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCHHHHHHH
AVVNTTLDMFLEFIAEVLDVNMCSVMLCDDMTGDLMIKGARGLDDHMIKQTRVRMGDRIA
HHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCCEE
GWVALEGEPLFIEDIEQDPRFGRKSVPQYNSKSLISLPLKVGGKVIGVLNLNNKRSASPF
EEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHCCCCEEEEEEECCCCCCCCCC
TGRDFDLAQAMCERVAYFMGRVHGGDHQGDEFRQLMTSFDSLITAEKKYHKKDPRLPDIA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
MGIAAQIGIGEDERRSILFASVLYDLGLVLLEESILNKKGKLLPSEENTLKIHPYTTVGL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEHH
LDSFEFSEEIKRGILHHHERYDGTGYPDGLKGDEIPLISRILSVVDAYCAMTSERPYRPR
HHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
MDMADALAEVHKHTGTLYDPKVVKALDFVVGEGVE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]