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The map label for this gene is purF [H]

Identifier: 78223146

GI number: 78223146

Start: 2163368

End: 2164768

Strand: Reverse

Name: purF [H]

Synonym: Gmet_1939

Alternate gene names: 78223146

Gene position: 2164768-2163368 (Counterclockwise)

Preceding gene: 78223147

Following gene: 78223145

Centisome position: 54.15

GC content: 61.96

Gene sequence:

>1401_bases
ATGAAGCTTTACAGACCTACGGAAGAGTGCGGCATTTTTGGAATTTATGGACATCCCGAGGCGGCAAACCTGACCTACCT
CGGACTTTACGCCCTCCAGCACCGGGGGCAGGAAGCCTGCGGCATTGTTTCCTCGGACGGACGGAGCCTCCACGTCCACC
GGAGCATGGGGCTGGTGGCCGATGTTTTCGGCGACCAGGCGATTTTCCGCAACCTCCCGGGTGAGGCGGCCATTGGGCAC
GTCCGCTACTCTACCACCGGCGACTCGGTCACCAAGAACGTCCAGCCCATCAAGGTCGATTATTCCCGGGGCTCCATTGC
CGTGGCCCACAACGGCAACCTGGTAAACGCCCAGATGGTCAAGGACGAGCTGGAAGCGTGGGGCTCCATCTTCCAGACCA
CTATGGACACCGAGGTGATCCTGCACCTCCTGGCCTCCTCCAAGCAGAACGCCCTTGAAGACCGCATCGCCGAGGCCCTC
GGCCGCCTGAAAGGGGCCTACTGCCTCCTCTTCCTGACCGAGACCCGCATGGTGGCTGCCCGCGATCCCCAGGGATTCCG
GCCCCTCTGTCTCGGCAAGCTCGGTGACGGCTGGGTGGTCGCCTCGGAGAGCTGTGCCCTGGACCTCATCGAGGCCGAAT
TCATCCGCGAGGTGGAGCCAGGAGAGGTCATCGTCATCACCAAGGACGGCATCACCTCCCATTTCCCCCTCAAGAAGTCC
GAGGCAGCCCCCTGCATCTTCGAATTCGTCTATTTCGCCCGTCCCGATTCCTACATCTTCGGAAAAAACGTCTATATGGT
CCGCAAGGATTTCGGTCGGCAACTGGCCCGGGAGCACCAGGTGGATGCCGATATCGTGATCCCGGTTCCCGATTCGGGGG
TGCCGGCGGCCCTGGGCTATGCCCAGGAGGCGGGGCTTCCCTTCGAACTGGGACTGATCCGCAACCACTACATCGGCCGG
ACCTTCATCGAACCCCAGCAGTCGATCCGCCACTTCGGGGTCAAGATCAAGCTGAACCCGGTGCGCGAGATCCTGGAGGG
AAAACGGGTCGTGGTCATCGACGACTCCATCGTCCGCGGCACCACCTCCCGCAAGATCGTCAAGATGGTGCGCAACGCCG
GCGCGAAGGAAGTTCATGTCCGGATTTCCTCCCCCCCTACCAGCTACCCCTGCTACTACGGCATCGATACCCCCACCCGG
AAGGAACTCATCTCCTCGTCCCACACCATCGAGGAAATCCGCCGGTACATCACCGCCGATTCCCTCGGCTACCTGTCGGA
GGAGGGGCTGCTGAAGGCAGTGGGGGCCGGTGAGAATCCCTTTTGCAAGGCTTGCTTCTCCGGCAACTACCCCATCACAT
TCCCTAAGCTGGCGGCTGCGCCCCAGCTGGACCTCTTCTGA

Upstream 100 bases:

>100_bases
TCAAGAACGCTGCCGAGTATGTCAAACTAAACTTGCTGTAAGACCGCTGAAACCAGGCGGCTGCCACCGTGCAGCCGCTT
ATTTCGTGCATGGAGACGGA

Downstream 100 bases:

>100_bases
AAGGAGCAGATTCGACCATGACTGACCGGGAGCGCTTGAAACAGATAATTATTGAACTCTCCTATGAAAAACGGAAGGTT
ACCCTCGCTTCGGGCCGCGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLHVHRSMGLVADVFGDQAIFRNLPGEAAIGH
VRYSTTGDSVTKNVQPIKVDYSRGSIAVAHNGNLVNAQMVKDELEAWGSIFQTTMDTEVILHLLASSKQNALEDRIAEAL
GRLKGAYCLLFLTETRMVAARDPQGFRPLCLGKLGDGWVVASESCALDLIEAEFIREVEPGEVIVITKDGITSHFPLKKS
EAAPCIFEFVYFARPDSYIFGKNVYMVRKDFGRQLAREHQVDADIVIPVPDSGVPAALGYAQEAGLPFELGLIRNHYIGR
TFIEPQQSIRHFGVKIKLNPVREILEGKRVVVIDDSIVRGTTSRKIVKMVRNAGAKEVHVRISSPPTSYPCYYGIDTPTR
KELISSSHTIEEIRRYITADSLGYLSEEGLLKAVGAGENPFCKACFSGNYPITFPKLAAAPQLDLF

Sequences:

>Translated_466_residues
MKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLHVHRSMGLVADVFGDQAIFRNLPGEAAIGH
VRYSTTGDSVTKNVQPIKVDYSRGSIAVAHNGNLVNAQMVKDELEAWGSIFQTTMDTEVILHLLASSKQNALEDRIAEAL
GRLKGAYCLLFLTETRMVAARDPQGFRPLCLGKLGDGWVVASESCALDLIEAEFIREVEPGEVIVITKDGITSHFPLKKS
EAAPCIFEFVYFARPDSYIFGKNVYMVRKDFGRQLAREHQVDADIVIPVPDSGVPAALGYAQEAGLPFELGLIRNHYIGR
TFIEPQQSIRHFGVKIKLNPVREILEGKRVVVIDDSIVRGTTSRKIVKMVRNAGAKEVHVRISSPPTSYPCYYGIDTPTR
KELISSSHTIEEIRRYITADSLGYLSEEGLLKAVGAGENPFCKACFSGNYPITFPKLAAAPQLDLF
>Mature_466_residues
MKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLHVHRSMGLVADVFGDQAIFRNLPGEAAIGH
VRYSTTGDSVTKNVQPIKVDYSRGSIAVAHNGNLVNAQMVKDELEAWGSIFQTTMDTEVILHLLASSKQNALEDRIAEAL
GRLKGAYCLLFLTETRMVAARDPQGFRPLCLGKLGDGWVVASESCALDLIEAEFIREVEPGEVIVITKDGITSHFPLKKS
EAAPCIFEFVYFARPDSYIFGKNVYMVRKDFGRQLAREHQVDADIVIPVPDSGVPAALGYAQEAGLPFELGLIRNHYIGR
TFIEPQQSIRHFGVKIKLNPVREILEGKRVVVIDDSIVRGTTSRKIVKMVRNAGAKEVHVRISSPPTSYPCYYGIDTPTR
KELISSSHTIEEIRRYITADSLGYLSEEGLLKAVGAGENPFCKACFSGNYPITFPKLAAAPQLDLF

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=40.3162055335968, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=468, Percent_Identity=41.6666666666667, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=29.8701298701299, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=41.0041841004184, Blast_Score=344, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6323958, Length=472, Percent_Identity=35.8050847457627, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=43.6734693877551, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=44.6120689655172, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=42.6829268292683, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51351; Mature: 51351

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLHVHRSMGLVA
CCCCCCCHHCCEEEEECCCCCCCEEEEEHHHHHHCCCHHCEEEECCCCEEEEECCCCHHH
DVFGDQAIFRNLPGEAAIGHVRYSTTGDSVTKNVQPIKVDYSRGSIAVAHNGNLVNAQMV
HHHCCHHHHHCCCCCCEEEEEEEECCCCHHHCCCCEEEEEECCCCEEEEECCCEEEHHHH
KDELEAWGSIFQTTMDTEVILHLLASSKQNALEDRIAEALGRLKGAYCLLFLTETRMVAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEE
RDPQGFRPLCLGKLGDGWVVASESCALDLIEAEFIREVEPGEVIVITKDGITSHFPLKKS
CCCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
EAAPCIFEFVYFARPDSYIFGKNVYMVRKDFGRQLAREHQVDADIVIPVPDSGVPAALGY
CCCCHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCC
AQEAGLPFELGLIRNHYIGRTFIEPQQSIRHFGVKIKLNPVREILEGKRVVVIDDSIVRG
HHHCCCCEEEHHHHCCCCCCCCCCHHHHHHHCCEEEEECHHHHHHCCCEEEEECCCHHCC
TTSRKIVKMVRNAGAKEVHVRISSPPTSYPCYYGIDTPTRKELISSSHTIEEIRRYITAD
CHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHHC
SLGYLSEEGLLKAVGAGENPFCKACFSGNYPITFPKLAAAPQLDLF
CCCCCCCCCCEEECCCCCCCCCHHEECCCCCEECHHHCCCCCCCCC
>Mature Secondary Structure
MKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLHVHRSMGLVA
CCCCCCCHHCCEEEEECCCCCCCEEEEEHHHHHHCCCHHCEEEECCCCEEEEECCCCHHH
DVFGDQAIFRNLPGEAAIGHVRYSTTGDSVTKNVQPIKVDYSRGSIAVAHNGNLVNAQMV
HHHCCHHHHHCCCCCCEEEEEEEECCCCHHHCCCCEEEEEECCCCEEEEECCCEEEHHHH
KDELEAWGSIFQTTMDTEVILHLLASSKQNALEDRIAEALGRLKGAYCLLFLTETRMVAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEE
RDPQGFRPLCLGKLGDGWVVASESCALDLIEAEFIREVEPGEVIVITKDGITSHFPLKKS
CCCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
EAAPCIFEFVYFARPDSYIFGKNVYMVRKDFGRQLAREHQVDADIVIPVPDSGVPAALGY
CCCCHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCC
AQEAGLPFELGLIRNHYIGRTFIEPQQSIRHFGVKIKLNPVREILEGKRVVVIDDSIVRG
HHHCCCCEEEHHHHCCCCCCCCCCHHHHHHHCCEEEEECHHHHHHCCCEEEEECCCHHCC
TTSRKIVKMVRNAGAKEVHVRISSPPTSYPCYYGIDTPTRKELISSSHTIEEIRRYITAD
CHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHHC
SLGYLSEEGLLKAVGAGENPFCKACFSGNYPITFPKLAAAPQLDLF
CCCCCCCCCCEEECCCCCCCCCHHEECCCCCEECHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]