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The map label for this gene is pyrE [H]

Identifier: 78223145

GI number: 78223145

Start: 2162802

End: 2163350

Strand: Reverse

Name: pyrE [H]

Synonym: Gmet_1938

Alternate gene names: 78223145

Gene position: 2163350-2162802 (Counterclockwise)

Preceding gene: 78223146

Following gene: 78223144

Centisome position: 54.12

GC content: 61.75

Gene sequence:

>549_bases
ATGACTGACCGGGAGCGCTTGAAACAGATAATTATTGAACTCTCCTATGAAAAACGGAAGGTTACCCTCGCTTCGGGCCG
CGAGAGCGACTTCTACTTTGACGGCAAGCAGACTACCCTCCATGCCGAGGGGGGGCTCCTGGTCGGCAAGCTCTTCTATG
AAGCCATCAAGGACGTGGAAAATGTCCAGGGGGTAGGGGGGATCACCCTGGGGGCCGATCCCATCGCCACCGCCACCTCC
ATTGCGGCGTTTCTGGACGGACGCCCCATGCACGCCTTCATTATCCGCAAGGAGCCCAAGGGGCACGGCACCGGCCAGTG
GCTCGAAGGGCGCAAGAACCTCCCCCCCGGGGCTCGGGTCGTTATCGTGGAGGACGTGGTCACCACCGGCGGTTCTTCCA
TGAAAGCAGTCCGGCGGGCCGAAGAGGAAGGGCTGGTCGTGCTCGGCATCGTCGCCCTCGTGGACCGTGAGGAGGGGGGC
CGCGAGAACATCGAGGCGGAAGGGGTCTGGCTGAGAAGCATTTTTACCAAGTCTCAGCTTGTGGCCTGA

Upstream 100 bases:

>100_bases
CCTTTTGCAAGGCTTGCTTCTCCGGCAACTACCCCATCACATTCCCTAAGCTGGCGGCTGCGCCCCAGCTGGACCTCTTC
TGAAAGGAGCAGATTCGACC

Downstream 100 bases:

>100_bases
AAAACGATTAGTGCATTGCGCAGAGAAGGCGGGAGTTTCCCGCCTTCTTTTTTTGAGGAGATATGAACGAGGTTCTCGAC
GAATACCGCAGACTGCTCTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MTDRERLKQIIIELSYEKRKVTLASGRESDFYFDGKQTTLHAEGGLLVGKLFYEAIKDVENVQGVGGITLGADPIATATS
IAAFLDGRPMHAFIIRKEPKGHGTGQWLEGRKNLPPGARVVIVEDVVTTGGSSMKAVRRAEEEGLVVLGIVALVDREEGG
RENIEAEGVWLRSIFTKSQLVA

Sequences:

>Translated_182_residues
MTDRERLKQIIIELSYEKRKVTLASGRESDFYFDGKQTTLHAEGGLLVGKLFYEAIKDVENVQGVGGITLGADPIATATS
IAAFLDGRPMHAFIIRKEPKGHGTGQWLEGRKNLPPGARVVIVEDVVTTGGSSMKAVRRAEEEGLVVLGIVALVDREEGG
RENIEAEGVWLRSIFTKSQLVA
>Mature_181_residues
TDRERLKQIIIELSYEKRKVTLASGRESDFYFDGKQTTLHAEGGLLVGKLFYEAIKDVENVQGVGGITLGADPIATATSI
AAFLDGRPMHAFIIRKEPKGHGTGQWLEGRKNLPPGARVVIVEDVVTTGGSSMKAVRRAEEEGLVVLGIVALVDREEGGR
ENIEAEGVWLRSIFTKSQLVA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=160, Percent_Identity=32.5, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=179, Percent_Identity=36.3128491620112, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17933654, Length=157, Percent_Identity=39.4904458598726, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 19819; Mature: 19688

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRERLKQIIIELSYEKRKVTLASGRESDFYFDGKQTTLHAEGGLLVGKLFYEAIKDVE
CCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHH
NVQGVGGITLGADPIATATSIAAFLDGRPMHAFIIRKEPKGHGTGQWLEGRKNLPPGARV
HCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
VIVEDVVTTGGSSMKAVRRAEEEGLVVLGIVALVDREEGGRENIEAEGVWLRSIFTKSQL
EEEEEEECCCCHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
VA
CC
>Mature Secondary Structure 
TDRERLKQIIIELSYEKRKVTLASGRESDFYFDGKQTTLHAEGGLLVGKLFYEAIKDVE
CCHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHH
NVQGVGGITLGADPIATATSIAAFLDGRPMHAFIIRKEPKGHGTGQWLEGRKNLPPGARV
HCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
VIVEDVVTTGGSSMKAVRRAEEEGLVVLGIVALVDREEGGRENIEAEGVWLRSIFTKSQL
EEEEEEECCCCHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
VA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA