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The map label for this gene is pleD [H]

Identifier: 78223121

GI number: 78223121

Start: 2136521

End: 2137900

Strand: Reverse

Name: pleD [H]

Synonym: Gmet_1914

Alternate gene names: 78223121

Gene position: 2137900-2136521 (Counterclockwise)

Preceding gene: 78223122

Following gene: 78223120

Centisome position: 53.48

GC content: 52.97

Gene sequence:

>1380_bases
ATGGATAAAATCCTCGTCGTCGAAGATGATCGCTTCTTTCGGCAGATGTATGTGGATCTCCTCAAGGAAGACGGATACGA
GGTAGATACGGTGACCTCCGGTGCCCGGGCGCTGGAGCTTCTTTCCGCCAACGATTATCAGCTTGTCATCACCGAGTTGG
TCATTCCCGGCATGAGTGGTATTGAACTCCTGACAAGGGTAAAGCAGCAGATGCCCACGGTGGATGTAATCCTCGTGACC
GGGCACGCAAATGTCGAATCTGCCGTGTTGGCCCTCAAAAGCGGCGCCCGTGATTACCTTCTCAAACCCTTCAATCATGA
AGAGTTCAAACATACCGTTGCTCTCTGCTTCGAGCAGCGTCGGCTCATCAACGAGAACTACGAACTGAAAGAGCTTCTGA
ACCTCTTCCAGATCGGCCAGAACATCGCCAACTGCATCGACATGGAACGCCTTTCAGCGGTAGTCCTTGACGCCCTGACA
AATGAGATCGGTGCTGACAGGGCCATTGGCCTGTTCCATCATAAGGACGATAATCTTGCCCTTAAGGAATTCAAGGGGTT
CAGCGAGGAGGCAGCTCAGAAACTTGCTGAGGCATTTCGGAAAAATTTCTGTATCGAGACCAATGATGGCTCGGTTATTA
ACAAGATTAATGCAACCCCCCTTGTGTCGGGAGCGGAATTCGACGGATTAGAGCTCTCGGAAGGGCTTCTTCTTGTCATA
CGTTCCAAGGGAGAGGTTCAGGGGGTAGTAGTCATTTTCAATAACCCCGGGCAGAAACTTCCGTTCGACTACAAGCAGAA
GAGCATTCAGTTTCTTCTTGAGCAGTCAACCCTCGCCTTTGAGAACGCCTCCCGCTACTCGAATGCCAGGGACATGCTCT
ATATCGATGAACTGACCGGGCTCTTCAACTACCGCTACCTGGATATCTCTCTCGATCGGGAGATCAAGCGGGCAGACCGC
TTCGGCTCTACGGTATCCATGATCTTCATTGATCTGGATTTCTTCAAGGGGGTAAACGACACCCACGGCCACCTGGTGGG
GAGTCAAGTCCTTAACGAAATGGGGATGCTCCTCAAGAAATCCGTACGCGAAGTGGATATCGTAATCCGGTACGGTGGCG
ACGAATTTACTGTCATGCTCGTGGAGACGGGGGAGAAGGGGGCCGCCACCGTGGCCGAGCGGATCCGCCGTTCCATCGAG
GGGCACACATTCCTGGCTGCCGAAGGATTCAACATTCGGCTCACTGCAAGCCTCGGCTACGCCTGTTATCCTGCCGACAC
CCAGTCCAAGCTCGAACTCCTCGAACTGGCCGACAAGGCCATGTACCAGGGGAAGGAGCAGGGTAAAAACTGTGTGTTCA
GGGCAACTGCAATTCGTTGA

Upstream 100 bases:

>100_bases
GGACGGGCATTTTCATTGACAAGCATCCCGTTTTATCTGATAGTTTTAAGGGTTAAAGCGCGGACACTGCACAAATTCGC
ACCCTCAGGAGCTACCCTCC

Downstream 100 bases:

>100_bases
TAATTTAGCTAGTTATTTAAAGTTTAAGGAGTAGGTAAGTACATGATCACTGGCATCAAGGGTTTTAACGATATTCTTCC
CGGCGAGGTGGAGCGGTGGC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDKILVVEDDRFFRQMYVDLLKEDGYEVDTVTSGARALELLSANDYQLVITELVIPGMSGIELLTRVKQQMPTVDVILVT
GHANVESAVLALKSGARDYLLKPFNHEEFKHTVALCFEQRRLINENYELKELLNLFQIGQNIANCIDMERLSAVVLDALT
NEIGADRAIGLFHHKDDNLALKEFKGFSEEAAQKLAEAFRKNFCIETNDGSVINKINATPLVSGAEFDGLELSEGLLLVI
RSKGEVQGVVVIFNNPGQKLPFDYKQKSIQFLLEQSTLAFENASRYSNARDMLYIDELTGLFNYRYLDISLDREIKRADR
FGSTVSMIFIDLDFFKGVNDTHGHLVGSQVLNEMGMLLKKSVREVDIVIRYGGDEFTVMLVETGEKGAATVAERIRRSIE
GHTFLAAEGFNIRLTASLGYACYPADTQSKLELLELADKAMYQGKEQGKNCVFRATAIR

Sequences:

>Translated_459_residues
MDKILVVEDDRFFRQMYVDLLKEDGYEVDTVTSGARALELLSANDYQLVITELVIPGMSGIELLTRVKQQMPTVDVILVT
GHANVESAVLALKSGARDYLLKPFNHEEFKHTVALCFEQRRLINENYELKELLNLFQIGQNIANCIDMERLSAVVLDALT
NEIGADRAIGLFHHKDDNLALKEFKGFSEEAAQKLAEAFRKNFCIETNDGSVINKINATPLVSGAEFDGLELSEGLLLVI
RSKGEVQGVVVIFNNPGQKLPFDYKQKSIQFLLEQSTLAFENASRYSNARDMLYIDELTGLFNYRYLDISLDREIKRADR
FGSTVSMIFIDLDFFKGVNDTHGHLVGSQVLNEMGMLLKKSVREVDIVIRYGGDEFTVMLVETGEKGAATVAERIRRSIE
GHTFLAAEGFNIRLTASLGYACYPADTQSKLELLELADKAMYQGKEQGKNCVFRATAIR
>Mature_459_residues
MDKILVVEDDRFFRQMYVDLLKEDGYEVDTVTSGARALELLSANDYQLVITELVIPGMSGIELLTRVKQQMPTVDVILVT
GHANVESAVLALKSGARDYLLKPFNHEEFKHTVALCFEQRRLINENYELKELLNLFQIGQNIANCIDMERLSAVVLDALT
NEIGADRAIGLFHHKDDNLALKEFKGFSEEAAQKLAEAFRKNFCIETNDGSVINKINATPLVSGAEFDGLELSEGLLLVI
RSKGEVQGVVVIFNNPGQKLPFDYKQKSIQFLLEQSTLAFENASRYSNARDMLYIDELTGLFNYRYLDISLDREIKRADR
FGSTVSMIFIDLDFFKGVNDTHGHLVGSQVLNEMGMLLKKSVREVDIVIRYGGDEFTVMLVETGEKGAATVAERIRRSIE
GHTFLAAEGFNIRLTASLGYACYPADTQSKLELLELADKAMYQGKEQGKNCVFRATAIR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=38.728323699422, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=35.4037267080745, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=33.7423312883436, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=36.6459627329193, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786584, Length=226, Percent_Identity=26.9911504424779, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=31.25, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082012, Length=132, Percent_Identity=35.6060606060606, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=105, Percent_Identity=37.1428571428571, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=33.5403726708075, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=157, Percent_Identity=31.8471337579618, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=36.1904761904762, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=36.4485981308411, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=149, Percent_Identity=30.2013422818792, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=37.2727272727273, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=31.7073170731707, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=30.0613496932515, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=32.7272727272727, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790863, Length=106, Percent_Identity=36.7924528301887, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=34.1666666666667, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787802, Length=139, Percent_Identity=30.2158273381295, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=118, Percent_Identity=35.5932203389831, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788905, Length=116, Percent_Identity=30.1724137931034, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51518; Mature: 51518

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKILVVEDDRFFRQMYVDLLKEDGYEVDTVTSGARALELLSANDYQLVITELVIPGMSG
CCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEEEEHHHCCCCCH
IELLTRVKQQMPTVDVILVTGHANVESAVLALKSGARDYLLKPFNHEEFKHTVALCFEQR
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHH
RLINENYELKELLNLFQIGQNIANCIDMERLSAVVLDALTNEIGADRAIGLFHHKDDNLA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
LKEFKGFSEEAAQKLAEAFRKNFCIETNDGSVINKINATPLVSGAEFDGLELSEGLLLVI
HHHHCCCCHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEE
RSKGEVQGVVVIFNNPGQKLPFDYKQKSIQFLLEQSTLAFENASRYSNARDMLYIDELTG
ECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHH
LFNYRYLDISLDREIKRADRFGSTVSMIFIDLDFFKGVNDTHGHLVGSQVLNEMGMLLKK
HHCEEEEEEECCHHHHHHHHCCCEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SVREVDIVIRYGGDEFTVMLVETGEKGAATVAERIRRSIEGHTFLAAEGFNIRLTASLGY
HHCEEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCE
ACYPADTQSKLELLELADKAMYQGKEQGKNCVFRATAIR
EEECCCCHHHHHHHHHHHHHHHCCHHCCCCEEEEEEECC
>Mature Secondary Structure
MDKILVVEDDRFFRQMYVDLLKEDGYEVDTVTSGARALELLSANDYQLVITELVIPGMSG
CCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEEEEHHHCCCCCH
IELLTRVKQQMPTVDVILVTGHANVESAVLALKSGARDYLLKPFNHEEFKHTVALCFEQR
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHH
RLINENYELKELLNLFQIGQNIANCIDMERLSAVVLDALTNEIGADRAIGLFHHKDDNLA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
LKEFKGFSEEAAQKLAEAFRKNFCIETNDGSVINKINATPLVSGAEFDGLELSEGLLLVI
HHHHCCCCHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEE
RSKGEVQGVVVIFNNPGQKLPFDYKQKSIQFLLEQSTLAFENASRYSNARDMLYIDELTG
ECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHH
LFNYRYLDISLDREIKRADRFGSTVSMIFIDLDFFKGVNDTHGHLVGSQVLNEMGMLLKK
HHCEEEEEEECCHHHHHHHHCCCEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SVREVDIVIRYGGDEFTVMLVETGEKGAATVAERIRRSIEGHTFLAAEGFNIRLTASLGY
HHCEEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCE
ACYPADTQSKLELLELADKAMYQGKEQGKNCVFRATAIR
EEECCCCHHHHHHHHHHHHHHHCCHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA