Click here to switch to the map view.

The map label for this gene is cysU [H]

Identifier: 78223112

GI number: 78223112

Start: 2126651

End: 2127487

Strand: Reverse

Name: cysU [H]

Synonym: Gmet_1905

Alternate gene names: 78223112

Gene position: 2127487-2126651 (Counterclockwise)

Preceding gene: 78223113

Following gene: 78223111

Centisome position: 53.22

GC content: 66.07

Gene sequence:

>837_bases
ATGATGGCAACCAAATCGACCAAGCGTACCACCGTCATCCCTGGCTTCGGCCCGACCCTCGGCTACACGCTCTTCTACCT
CTCCCTGATGGTGCTGCTGCCGCTCTCGTCCCTCGTGTTCAAGACGACAAGCCTCACCTGGCCCGAGTTCCTCGCCACGG
TCACGGCGCTGCGGGTGGTGGCCTCCTACCGGGTCACCTTCGGCGCGGCCCTGGCGGCTGCCGGGGTGAACGCCCTCTTC
GGGCTCCTGGTGGCGTGGGTGCTGGTGCGCTACCGGCTGCCGGGGAAGCGGCTCATCGACGCCCTGGTGGATCTCCCCTT
TGCGCTTCCCACGGCCGTGGCCGGCATCACCCTCTCCTCGGTCTACGCCGCCAACGGCTGGCTCGGGTCGATCCTTGAGC
CCCGGGGGATCAAGGTCGCCTTTACGCCGCTCGGGATCTTCGTGGCCATGATCTTCATCGGCCTCCCCTTCGTGGTGCGG
ACGGTGCAGCCGGTTCTGGAGGAACTGGAGGCAGAGATCGAAGAGGCGGCCGCCTGCCTCGGCGCCACCCGTTGGCAGAC
CTTCCGGCGCGTGCTCTTCCCGGAGCTCCTCCCAGCGCTCCTCACCGGTTTTGCCCTCGCCTTCGCCCGCGGGGTGGGGG
AGTACGGCTCTATCATCTTCATCGCCGGCAACATGCCGATGGTGTCGGAGATCACGCCGCTCCTCATTATCACCAAGCTG
GAGCAGTACGACTATGTCGGCGCCACCGCCATCGCCACGGTGATGCTCCTCTTCTCATTCCTCATGCTTCTCACCGTGAA
CCTCCTCCAGAAATGGAGCAGGCGCCACGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCGTAGCGCTGCTACGCCTCCGCAGTCCCTCCTGCGGGTGCGACGATCTGGCACAAACTCCACAGCCCTGCACTGGAT
TATGCAAGGGATCAACCACC

Downstream 100 bases:

>100_bases
TCGGGAGACTACGACATGTCCAGCATTTCTGCAAAATCAGCCCGCACCGAGCCGGCTCTCGTGCGCTGGTTGCTCATCGT
CACGGCGTTCCTCTTCCTGG

Product: Sulfate ABC transporter permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MMATKSTKRTTVIPGFGPTLGYTLFYLSLMVLLPLSSLVFKTTSLTWPEFLATVTALRVVASYRVTFGAALAAAGVNALF
GLLVAWVLVRYRLPGKRLIDALVDLPFALPTAVAGITLSSVYAANGWLGSILEPRGIKVAFTPLGIFVAMIFIGLPFVVR
TVQPVLEELEAEIEEAAACLGATRWQTFRRVLFPELLPALLTGFALAFARGVGEYGSIIFIAGNMPMVSEITPLLIITKL
EQYDYVGATAIATVMLLFSFLMLLTVNLLQKWSRRHAA

Sequences:

>Translated_278_residues
MMATKSTKRTTVIPGFGPTLGYTLFYLSLMVLLPLSSLVFKTTSLTWPEFLATVTALRVVASYRVTFGAALAAAGVNALF
GLLVAWVLVRYRLPGKRLIDALVDLPFALPTAVAGITLSSVYAANGWLGSILEPRGIKVAFTPLGIFVAMIFIGLPFVVR
TVQPVLEELEAEIEEAAACLGATRWQTFRRVLFPELLPALLTGFALAFARGVGEYGSIIFIAGNMPMVSEITPLLIITKL
EQYDYVGATAIATVMLLFSFLMLLTVNLLQKWSRRHAA
>Mature_278_residues
MMATKSTKRTTVIPGFGPTLGYTLFYLSLMVLLPLSSLVFKTTSLTWPEFLATVTALRVVASYRVTFGAALAAAGVNALF
GLLVAWVLVRYRLPGKRLIDALVDLPFALPTAVAGITLSSVYAANGWLGSILEPRGIKVAFTPLGIFVAMIFIGLPFVVR
TVQPVLEELEAEIEEAAACLGATRWQTFRRVLFPELLPALLTGFALAFARGVGEYGSIIFIAGNMPMVSEITPLLIITKL
EQYDYVGATAIATVMLLFSFLMLLTVNLLQKWSRRHAA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=271, Percent_Identity=52.7675276752767, Blast_Score=250, Evalue=6e-68,
Organism=Escherichia coli, GI87082099, Length=201, Percent_Identity=36.81592039801, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786980, Length=236, Percent_Identity=32.6271186440678, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786254, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787368, Length=225, Percent_Identity=28, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30195; Mature: 30195

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMATKSTKRTTVIPGFGPTLGYTLFYLSLMVLLPLSSLVFKTTSLTWPEFLATVTALRVV
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASYRVTFGAALAAAGVNALFGLLVAWVLVRYRLPGKRLIDALVDLPFALPTAVAGITLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
VYAANGWLGSILEPRGIKVAFTPLGIFVAMIFIGLPFVVRTVQPVLEELEAEIEEAAACL
HHHHCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GATRWQTFRRVLFPELLPALLTGFALAFARGVGEYGSIIFIAGNMPMVSEITPLLIITKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
EQYDYVGATAIATVMLLFSFLMLLTVNLLQKWSRRHAA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMATKSTKRTTVIPGFGPTLGYTLFYLSLMVLLPLSSLVFKTTSLTWPEFLATVTALRVV
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASYRVTFGAALAAAGVNALFGLLVAWVLVRYRLPGKRLIDALVDLPFALPTAVAGITLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
VYAANGWLGSILEPRGIKVAFTPLGIFVAMIFIGLPFVVRTVQPVLEELEAEIEEAAACL
HHHHCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GATRWQTFRRVLFPELLPALLTGFALAFARGVGEYGSIIFIAGNMPMVSEITPLLIITKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
EQYDYVGATAIATVMLLFSFLMLLTVNLLQKWSRRHAA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]