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The map label for this gene is cysW [H]

Identifier: 78223111

GI number: 78223111

Start: 2125799

End: 2126635

Strand: Reverse

Name: cysW [H]

Synonym: Gmet_1904

Alternate gene names: 78223111

Gene position: 2126635-2125799 (Counterclockwise)

Preceding gene: 78223112

Following gene: 78223110

Centisome position: 53.2

GC content: 64.28

Gene sequence:

>837_bases
ATGTCCAGCATTTCTGCAAAATCAGCCCGCACCGAGCCGGCTCTCGTGCGCTGGTTGCTCATCGTCACGGCGTTCCTCTT
CCTGGGCCTCTTCCTCTTCGTCCCCTTGGCGGCGGTTTTCGTCCAAGCCCTGGAGAAGGGATGGGGCGCGTATCTCTCCG
CCATCCGCGAGCCCGACGCCCTGGCGGCGGTGAAGCTGACTCTCGTCACCGCAGCGGTGAGCGTGCCGGTGAACCTGGTC
TTCGGGGTGGTGGCCGCCTGGGCCATCGCCAAGTTCGAGTTCCGGGGGAAGCAACTCCTCATCACCCTCATCGACCTGCC
GTTCTCGGTGTCGCCGGTCATTTCCGGGCTCATCTACGTGCTCCTCTTCGGGCTCCAGGGTTGGCTCGGGCCGTGGCTCC
AGGCCCATGACCTGAAGATCATCTTCGCCGTGCCGGGGATCGTCCTCGCCACGGTCTTCGTCACCTTCCCCTTCGTAGCC
CGGGAGCTGATTCCGCTCATGGAGGCCCAGGGGAAGGACGAGGAGGAGGCGGCCATTACCCTTGGCGCGAGCGGCTTCCA
AGCGTTCTGGCGGGTGACGATACCCAACATCAAGTGGGGGCTCCTCTACGGGGTGATCCTCTGCAACGCCCGGGCAATGG
GGGAGTTCGGTGCCGTCTCGGTGGTTTCCGGCCATATCCGGGGGGCGACCAACACGATCCCCCTCCACGTGGAGATCCTC
TACAACGAATACAACTACGTTGCCGCCTTTGCCGTGGCCTCGCTCCTGGCGCTTCTGGCGCTGGTCACTCTGGTGGCGAA
GAGTTTCGTCGAGTGGCGGATGCGCGAGAGCGCTTAA

Upstream 100 bases:

>100_bases
CGCCACGGTGATGCTCCTCTTCTCATTCCTCATGCTTCTCACCGTGAACCTCCTCCAGAAATGGAGCAGGCGCCACGCGG
CCTGATCGGGAGACTACGAC

Downstream 100 bases:

>100_bases
CCTGATCGCCAAGACCATCACAAAGGAACCATGACATGAGCATAGAGATTGTCAGTGTCAACAAAGCTTTTGGCAGCTTC
ACCGCCCTGAACGACGTGAA

Product: Sulfate ABC transporter permease CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSSISAKSARTEPALVRWLLIVTAFLFLGLFLFVPLAAVFVQALEKGWGAYLSAIREPDALAAVKLTLVTAAVSVPVNLV
FGVVAAWAIAKFEFRGKQLLITLIDLPFSVSPVISGLIYVLLFGLQGWLGPWLQAHDLKIIFAVPGIVLATVFVTFPFVA
RELIPLMEAQGKDEEEAAITLGASGFQAFWRVTIPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGATNTIPLHVEIL
YNEYNYVAAFAVASLLALLALVTLVAKSFVEWRMRESA

Sequences:

>Translated_278_residues
MSSISAKSARTEPALVRWLLIVTAFLFLGLFLFVPLAAVFVQALEKGWGAYLSAIREPDALAAVKLTLVTAAVSVPVNLV
FGVVAAWAIAKFEFRGKQLLITLIDLPFSVSPVISGLIYVLLFGLQGWLGPWLQAHDLKIIFAVPGIVLATVFVTFPFVA
RELIPLMEAQGKDEEEAAITLGASGFQAFWRVTIPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGATNTIPLHVEIL
YNEYNYVAAFAVASLLALLALVTLVAKSFVEWRMRESA
>Mature_277_residues
SSISAKSARTEPALVRWLLIVTAFLFLGLFLFVPLAAVFVQALEKGWGAYLSAIREPDALAAVKLTLVTAAVSVPVNLVF
GVVAAWAIAKFEFRGKQLLITLIDLPFSVSPVISGLIYVLLFGLQGWLGPWLQAHDLKIIFAVPGIVLATVFVTFPFVAR
ELIPLMEAQGKDEEEAAITLGASGFQAFWRVTIPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGATNTIPLHVEILY
NEYNYVAAFAVASLLALLALVTLVAKSFVEWRMRESA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=54.0229885057471, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1788764, Length=216, Percent_Identity=37.962962962963, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=30.7339449541284, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787369, Length=170, Percent_Identity=30.5882352941176, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30149; Mature: 30018

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSISAKSARTEPALVRWLLIVTAFLFLGLFLFVPLAAVFVQALEKGWGAYLSAIREPDA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LAAVKLTLVTAAVSVPVNLVFGVVAAWAIAKFEFRGKQLLITLIDLPFSVSPVISGLIYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
LLFGLQGWLGPWLQAHDLKIIFAVPGIVLATVFVTFPFVARELIPLMEAQGKDEEEAAIT
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEE
LGASGFQAFWRVTIPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGATNTIPLHVEIL
ECCHHHHHHEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHCCCCCCCCCEEEEEEEE
YNEYNYVAAFAVASLLALLALVTLVAKSFVEWRMRESA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSISAKSARTEPALVRWLLIVTAFLFLGLFLFVPLAAVFVQALEKGWGAYLSAIREPDA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LAAVKLTLVTAAVSVPVNLVFGVVAAWAIAKFEFRGKQLLITLIDLPFSVSPVISGLIYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
LLFGLQGWLGPWLQAHDLKIIFAVPGIVLATVFVTFPFVARELIPLMEAQGKDEEEAAIT
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEE
LGASGFQAFWRVTIPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGATNTIPLHVEIL
ECCHHHHHHEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHCCCCCCCCCEEEEEEEE
YNEYNYVAAFAVASLLALLALVTLVAKSFVEWRMRESA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]