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The map label for this gene is sbp [H]

Identifier: 78223113

GI number: 78223113

Start: 2127648

End: 2128661

Strand: Reverse

Name: sbp [H]

Synonym: Gmet_1906

Alternate gene names: 78223113

Gene position: 2128661-2127648 (Counterclockwise)

Preceding gene: 78223114

Following gene: 78223112

Centisome position: 53.25

GC content: 62.82

Gene sequence:

>1014_bases
ATGAAACCGATCAGAACCATCGCCACCCTCGCCGCAGCACTCCTCGCCCTTGCCGCCCCTCTGGCGGCCCGGGCCGAGGT
GAACCTCCTCAACGTCTCCTACGACCCGACTCGGGAGCTCTACCAGGACTATAACGCCGCCTTCGCCAGGTACTGGAAAG
GGAAGGGGGGCGACACAGTGACGGTGAAGCAGTCCCACGGTGGCTCGGGGAAACAGGCCCGGTCAGTCATCGACGGCCTG
GAGGCGGATGTGGTGACCCTGGCGCTCGCCTACGACATCGACGAGATCGGAGAGAGGGGGAAGCTCATCCCCGCCAACTG
GCAGAAGCGCCTTCCCCACAACAGTTCCCCCTACACCTCCACCATTGTCTTTCTCGTTCGCAAGGGAAATCCCAAGAAGA
TTCGTGACTGGAACGACCTCGTCCGCCCCGACGTGAAGGTGATCACTCCCAACCCCAAGACCTCCGGTGGCGCCCGCTGG
AACTATCTGGCCGCCTGGGGGTACGCCCTGAAGCAGAAGGGAGGGAGCGAGGCCAAGGCGAAGGAGTTTGTGACCAGGCT
CTTCAGGAACGTACCGGTGCTCGATTCCGGCGCCCGGGGCTCCACCACCACCTTTGTCCAGCGGGGACAGGGCGATGTGC
TCCTGGCGTGGGAGAACGAGGCGTTTCTGGCAGTGAACGAGCTGGGCAGGGATAAATTCCAGATCGTGGTGCCGTCGGTC
AGCATCCTGGCCGAGCCGCCGGTAACGGTGGTGGACAAGGTGGTGGACCGGAAGGGGACCCGCAAGGTGGCCGAGGAATA
CCTGAAGTACCTCTATACCCCGGCCGGGCAGGAAATTGCGGCCAGGCACTACTATCGCCCCATCGATAAGAAGGTGGCGG
CCAGGTACGCCAAGGTCTTCCCGAAGGTGAAGCTCTTCACCATTGACGATACCTTCGGCGGTTGGAAGGCGGCCCAGAAG
AAGCACTTCGCCGACGGCGGGGTGTTCGACCAGATCTACGGGCCGGGAAAATAA

Upstream 100 bases:

>100_bases
AGAAGGACGGGGCGCTTTTTTATGCAATCTGAAAGCAGCATTTTTTTGTGTCGTAAAAGACTATAAAATCGATAGGCTAA
AACAACAAAAGGAGCAGACC

Downstream 100 bases:

>100_bases
CGTGATGTAGGGGCTGCGGATTTCACACCATCTCGCCGCCGTCCTCGTCGCTCCTTTGTGCGGCGTAGCGCTGCTACGCC
TCCGCAGTCCCTCCTGCGGG

Product: thiosulphate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKPIRTIATLAAALLALAAPLAARAEVNLLNVSYDPTRELYQDYNAAFARYWKGKGGDTVTVKQSHGGSGKQARSVIDGL
EADVVTLALAYDIDEIGERGKLIPANWQKRLPHNSSPYTSTIVFLVRKGNPKKIRDWNDLVRPDVKVITPNPKTSGGARW
NYLAAWGYALKQKGGSEAKAKEFVTRLFRNVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGRDKFQIVVPSV
SILAEPPVTVVDKVVDRKGTRKVAEEYLKYLYTPAGQEIAARHYYRPIDKKVAARYAKVFPKVKLFTIDDTFGGWKAAQK
KHFADGGVFDQIYGPGK

Sequences:

>Translated_337_residues
MKPIRTIATLAAALLALAAPLAARAEVNLLNVSYDPTRELYQDYNAAFARYWKGKGGDTVTVKQSHGGSGKQARSVIDGL
EADVVTLALAYDIDEIGERGKLIPANWQKRLPHNSSPYTSTIVFLVRKGNPKKIRDWNDLVRPDVKVITPNPKTSGGARW
NYLAAWGYALKQKGGSEAKAKEFVTRLFRNVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGRDKFQIVVPSV
SILAEPPVTVVDKVVDRKGTRKVAEEYLKYLYTPAGQEIAARHYYRPIDKKVAARYAKVFPKVKLFTIDDTFGGWKAAQK
KHFADGGVFDQIYGPGK
>Mature_337_residues
MKPIRTIATLAAALLALAAPLAARAEVNLLNVSYDPTRELYQDYNAAFARYWKGKGGDTVTVKQSHGGSGKQARSVIDGL
EADVVTLALAYDIDEIGERGKLIPANWQKRLPHNSSPYTSTIVFLVRKGNPKKIRDWNDLVRPDVKVITPNPKTSGGARW
NYLAAWGYALKQKGGSEAKAKEFVTRLFRNVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGRDKFQIVVPSV
SILAEPPVTVVDKVVDRKGTRKVAEEYLKYLYTPAGQEIAARHYYRPIDKKVAARYAKVFPKVKLFTIDDTFGGWKAAQK
KHFADGGVFDQIYGPGK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=316, Percent_Identity=70.253164556962, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=50.3225806451613, Blast_Score=321, Evalue=4e-89,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37225; Mature: 37225

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIRTIATLAAALLALAAPLAARAEVNLLNVSYDPTRELYQDYNAAFARYWKGKGGDTV
CCHHHHHHHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
TVKQSHGGSGKQARSVIDGLEADVVTLALAYDIDEIGERGKLIPANWQKRLPHNSSPYTS
EEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCCCCHHHHCCCCCCCCCE
TIVFLVRKGNPKKIRDWNDLVRPDVKVITPNPKTSGGARWNYLAAWGYALKQKGGSEAKA
EEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHH
KEFVTRLFRNVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGRDKFQIVVPSV
HHHHHHHHHCCCCCCCCCCCCCHHHEECCCCCEEEEECCCEEEEHHHCCCCEEEEEECCH
SILAEPPVTVVDKVVDRKGTRKVAEEYLKYLYTPAGQEIAARHYYRPIDKKVAARYAKVF
HHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
PKVKLFTIDDTFGGWKAAQKKHFADGGVFDQIYGPGK
CCEEEEEEECCCCCHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure
MKPIRTIATLAAALLALAAPLAARAEVNLLNVSYDPTRELYQDYNAAFARYWKGKGGDTV
CCHHHHHHHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
TVKQSHGGSGKQARSVIDGLEADVVTLALAYDIDEIGERGKLIPANWQKRLPHNSSPYTS
EEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCCCCHHHHCCCCCCCCCE
TIVFLVRKGNPKKIRDWNDLVRPDVKVITPNPKTSGGARWNYLAAWGYALKQKGGSEAKA
EEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHH
KEFVTRLFRNVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGRDKFQIVVPSV
HHHHHHHHHCCCCCCCCCCCCCHHHEECCCCCEEEEECCCEEEEHHHCCCCEEEEEECCH
SILAEPPVTVVDKVVDRKGTRKVAEEYLKYLYTPAGQEIAARHYYRPIDKKVAARYAKVF
HHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
PKVKLFTIDDTFGGWKAAQKKHFADGGVFDQIYGPGK
CCEEEEEEECCCCCHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]