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The map label for this gene is gmd [H]

Identifier: 78222700

GI number: 78222700

Start: 1682727

End: 1683776

Strand: Direct

Name: gmd [H]

Synonym: Gmet_1488

Alternate gene names: 78222700

Gene position: 1682727-1683776 (Clockwise)

Preceding gene: 78222699

Following gene: 78222701

Centisome position: 42.1

GC content: 55.52

Gene sequence:

>1050_bases
GTGAAAAAAGCACTGATTACCGGCATCACCGGTCAGGATGGCTCCTACCTGGCGGAGTTGCTGCTGCAAAAGGGTTACGA
AGTCCACGGCATGATCCGCCGTTCGTCATCCTTTAACACAGGTCGGATTAACCACATCTACCGCGATCCACACGAAAAGG
ACGTCCGGTTGTTTTTGCACTATGGTGATCTCAACGACGCCAGTTCCATCAACACCCTGCTGCGCATCGTCCAACCGGAC
GAGATCTACAATCTTGGTGCCCAGAGCCATGTCCGGGTCTCATTCGATGTGCCGGAATACACCGGAGAGGTGGATGCCCT
GGGAGCGGTACGGATACTCGAGGGAATCCGGGAAACCGGACTTAATACCAGATTTTATCAGGCGTCATCTTCTGAACTCT
ATGGAAAGGTCGTTGAGACACCACAGCGTGAATCGACTCCATTTTACCCCCGGTCCCCCTACGGTTGTGCCAAGGCTTAT
GCCTACTACATCACCCAGAACTATCGCGAAAGCTACGACATTTACGCCTGCAACGGCATTCTCTTCAATCATGAGTCACC
TCGGCGTGGCGAAACCTTTGTTACCCGAAAGATCACCCGGGCGGCGGCCCGGATCAAGTTGGGGCTCCAGAACTGTCTCT
ATCTGGGGAACCTGGATGCAAAACGGGACTGGGGATTTGCCGGAGATTACGTTGAAGCGATGTGGCTGATGCTGCAACAG
GAGCAGGCGGACGACTATGTTATCGCCACCGGCGAGACCTATATGGTCCGAACCTTCGCCGAAAAGGTTTTCGCCCGCCT
TGGGATGCCGCTTGAATGGGGGGGGAGCGGCGATCGGGAAGAAGGGGTTGACACCGCTACCGGCAAGCCTCTGATTCGCG
TTGACTCCAAATATTTCCGTCCCGCAGAGGTTGACTTGCTACTGGGAGACCCATCAAAGGCAAAGCGGGTGCTCGGCTGG
CAACAGCAGACCAGCTTTGACCAGTTGGTCGCCATGATGACCGATGCCGATCTGGCGCTGGCAGAGCGGGAAATGCGCGC
CAATGGATAA

Upstream 100 bases:

>100_bases
CCGCGGAACCTGGCAAAGAGTGTGACGGTTGAATGAAGGGCATGAATTTGTCAGGTGGCTCTGCTTCAGAACCAGCAATG
TATTGAAAAGAGGTAGGAAC

Downstream 100 bases:

>100_bases
ATTGAATGTAATGTTTGTGTTCGGAACTCGCCCCGAGGCCATAAAAATGGCGCCGTTGGTGAAGGAACTGCAGAAGCAAG
CCGGACAGTTCAAGACCATA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKKALITGITGQDGSYLAELLLQKGYEVHGMIRRSSSFNTGRINHIYRDPHEKDVRLFLHYGDLNDASSINTLLRIVQPD
EIYNLGAQSHVRVSFDVPEYTGEVDALGAVRILEGIRETGLNTRFYQASSSELYGKVVETPQRESTPFYPRSPYGCAKAY
AYYITQNYRESYDIYACNGILFNHESPRRGETFVTRKITRAAARIKLGLQNCLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EQADDYVIATGETYMVRTFAEKVFARLGMPLEWGGSGDREEGVDTATGKPLIRVDSKYFRPAEVDLLLGDPSKAKRVLGW
QQQTSFDQLVAMMTDADLALAEREMRANG

Sequences:

>Translated_349_residues
MKKALITGITGQDGSYLAELLLQKGYEVHGMIRRSSSFNTGRINHIYRDPHEKDVRLFLHYGDLNDASSINTLLRIVQPD
EIYNLGAQSHVRVSFDVPEYTGEVDALGAVRILEGIRETGLNTRFYQASSSELYGKVVETPQRESTPFYPRSPYGCAKAY
AYYITQNYRESYDIYACNGILFNHESPRRGETFVTRKITRAAARIKLGLQNCLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EQADDYVIATGETYMVRTFAEKVFARLGMPLEWGGSGDREEGVDTATGKPLIRVDSKYFRPAEVDLLLGDPSKAKRVLGW
QQQTSFDQLVAMMTDADLALAEREMRANG
>Mature_349_residues
MKKALITGITGQDGSYLAELLLQKGYEVHGMIRRSSSFNTGRINHIYRDPHEKDVRLFLHYGDLNDASSINTLLRIVQPD
EIYNLGAQSHVRVSFDVPEYTGEVDALGAVRILEGIRETGLNTRFYQASSSELYGKVVETPQRESTPFYPRSPYGCAKAY
AYYITQNYRESYDIYACNGILFNHESPRRGETFVTRKITRAAARIKLGLQNCLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EQADDYVIATGETYMVRTFAEKVFARLGMPLEWGGSGDREEGVDTATGKPLIRVDSKYFRPAEVDLLLGDPSKAKRVLGW
QQQTSFDQLVAMMTDADLALAEREMRANG

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=59.1954022988506, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=61.864406779661, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=25.1445086705202, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=22.7011494252874, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=351, Percent_Identity=57.5498575498575, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI133901786, Length=351, Percent_Identity=57.5498575498575, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI133901788, Length=351, Percent_Identity=57.5498575498575, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17539422, Length=351, Percent_Identity=57.5498575498575, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI133901790, Length=351, Percent_Identity=57.5498575498575, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17507723, Length=345, Percent_Identity=58.8405797101449, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24158427, Length=350, Percent_Identity=62, Blast_Score=448, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39458; Mature: 39458

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGSYLAELLLQKGYEVHGMIRRSSSFNTGRINHIYRDPHEKDVRLFLH
CCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEE
YGDLNDASSINTLLRIVQPDEIYNLGAQSHVRVSFDVPEYTGEVDALGAVRILEGIRETG
ECCCCCHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
LNTRFYQASSSELYGKVVETPQRESTPFYPRSPYGCAKAYAYYITQNYRESYDIYACNGI
CCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECEE
LFNHESPRRGETFVTRKITRAAARIKLGLQNCLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
EQADDYVIATGETYMVRTFAEKVFARLGMPLEWGGSGDREEGVDTATGKPLIRVDSKYFR
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCC
PAEVDLLLGDPSKAKRVLGWQQQTSFDQLVAMMTDADLALAEREMRANG
CCEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKALITGITGQDGSYLAELLLQKGYEVHGMIRRSSSFNTGRINHIYRDPHEKDVRLFLH
CCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEE
YGDLNDASSINTLLRIVQPDEIYNLGAQSHVRVSFDVPEYTGEVDALGAVRILEGIRETG
ECCCCCHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
LNTRFYQASSSELYGKVVETPQRESTPFYPRSPYGCAKAYAYYITQNYRESYDIYACNGI
CCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECEE
LFNHESPRRGETFVTRKITRAAARIKLGLQNCLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
EQADDYVIATGETYMVRTFAEKVFARLGMPLEWGGSGDREEGVDTATGKPLIRVDSKYFR
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCC
PAEVDLLLGDPSKAKRVLGWQQQTSFDQLVAMMTDADLALAEREMRANG
CCEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]