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The map label for this gene is murE

Identifier: 78221628

GI number: 78221628

Start: 449113

End: 450648

Strand: Direct

Name: murE

Synonym: Gmet_0407

Alternate gene names: 78221628

Gene position: 449113-450648 (Clockwise)

Preceding gene: 78221627

Following gene: 78221629

Centisome position: 11.24

GC content: 61.39

Gene sequence:

>1536_bases
ATGAGACTGGAAGATCTGGCACGGGTCATTAATCCCTCTGAAGCAGGAGGATCTCTGGCGCTGGAGATCGAAGGGCTCTA
CTGCGATTCACGGCAGGTGCGGTCCGGCGGTCTCTTTTTCGCGTTGAGGGGCGTGGCGACAGACGGCCATGATTACATTG
TCGCCTCCCGCGACCGTGGCGCGGTTGCCGTGGTGGTGGAGGATCCGTCGCGGGTCCCCGAAGGAATGACCTGGCTGAAG
GTCGATGACGCGCGTCTTGCCATGTCCCGTGCTGCCGCAGCCTTCTACGGCAACCCCACCGATGGTATTCCCGTGGTCGG
TATCACGGGGACCAACGGAAAGACGACCACTACGTACCTGGTGGAGGCCGTCATGGCCCGAGCCGGCATCCCTGTGGCGG
TTCTCGGGACTATCAGTTACCGCTTCGGTGACAAGACTATCCCGGCTCCCCACACGACTCCCGAGTCGGTAGATCTGCAG
CGGACCATACGCGATCTGGTGGACCGGGGCGCCCAAGGTGTGGTCATGGAGGTTTCCTCCCATGCCCTTGAGCAGCGACG
GGTGGATGGATGCCGCTTCGATGTCGGCGTCTTCACCAATCTCACCCGTGACCACCTGGACTATCATCGGGACATGGAGT
CGTACTTCGAAAGCAAGGCGCGGCTATTTACCGAGCTTCTGGCAGCGGACGACACGAAACCCCGACGTGCCGCGGTTATT
AACGTGGACGACCCCTATGGCGCCCGACTCGCCAGTGACGCGGTTGCGCCGGTGGTGAGCTACGGGCTGTCTCCTTCAGT
CATGGTCCGGGCCGAGATGGTCACCTTTTCTGTCGATGGAATCTCTGGTACCCTCGTTACCCCCCTTGGTACGATACCGT
TTCACTCGCGGCTTCTGGGGCGGTTCAACCTCTACAACATCCTGGCGGCTGTGGCGGCTGGCGTGGCCCTGGGACTCTCC
CCGGATGCAATTCGCGGGGGAATCGAGGGGGATGTCCGGGTCCCTGGACGCCTTGAGCGGGTTGATAACGACCATGGGAT
CACGGTGCTGGTCGATTATGCCCACACGGGCGACGCCCTGGAGAATGTCCTGAAGACCGTTGCCGAGATCGCCACCGGCC
GGATCATCACTGTATTCGGCTGCGGCGGAGACCGGGATCGGGGAAAACGGCCCGTCATGGGGGAGATTGCAGGGCGTTTC
AGCGATCTATCCATCGTCACCTCCGACAACCCGCGAACCGAGGATGCCGGAGCGATTATTGGCGAGATTCTCGGCGGTAT
CCGTTCTCTTGGGCTCCGGGAGTATGGGGCGGAAGAACTCATCACCGGTTTCGATGCCAAAGGATTTGCTGCGGTGGAAT
CGAGACGCGAGGCAATCAGGCTGGCCGCCCGCGTGGCACGTCCTGGGGATGTTCTGCTCCTCGCTGGCAAGGGACACGAG
GACTACCAGATTATCGGGACGATGAAGCACCACTTCGACGATCGGGAAGAGGTTGCTGCCGCTTTTCGGGAACTGTCATC
CAAAGGACAGGGATAG

Upstream 100 bases:

>100_bases
GCCCAGCGACCAGGTTTGGGTCAAGTTCACGCCGACGGCGTAACAAGGCTGGCTGAAGAAAGATGAAAATGAGCACAAGT
AAATTCTATGGCGAGGGAAC

Downstream 100 bases:

>100_bases
CGTGTTATTCTCTCTGGCAGACATACGTGAGGCAACGGGAGGCACCCTCGGCGGCGAAGGGAAAACTGCCGGTGTGGAGG
GGGTGTCAACCGACTCCCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRLEDLARVINPSEAGGSLALEIEGLYCDSRQVRSGGLFFALRGVATDGHDYIVASRDRGAVAVVVEDPSRVPEGMTWLK
VDDARLAMSRAAAAFYGNPTDGIPVVGITGTNGKTTTTYLVEAVMARAGIPVAVLGTISYRFGDKTIPAPHTTPESVDLQ
RTIRDLVDRGAQGVVMEVSSHALEQRRVDGCRFDVGVFTNLTRDHLDYHRDMESYFESKARLFTELLAADDTKPRRAAVI
NVDDPYGARLASDAVAPVVSYGLSPSVMVRAEMVTFSVDGISGTLVTPLGTIPFHSRLLGRFNLYNILAAVAAGVALGLS
PDAIRGGIEGDVRVPGRLERVDNDHGITVLVDYAHTGDALENVLKTVAEIATGRIITVFGCGGDRDRGKRPVMGEIAGRF
SDLSIVTSDNPRTEDAGAIIGEILGGIRSLGLREYGAEELITGFDAKGFAAVESRREAIRLAARVARPGDVLLLAGKGHE
DYQIIGTMKHHFDDREEVAAAFRELSSKGQG

Sequences:

>Translated_511_residues
MRLEDLARVINPSEAGGSLALEIEGLYCDSRQVRSGGLFFALRGVATDGHDYIVASRDRGAVAVVVEDPSRVPEGMTWLK
VDDARLAMSRAAAAFYGNPTDGIPVVGITGTNGKTTTTYLVEAVMARAGIPVAVLGTISYRFGDKTIPAPHTTPESVDLQ
RTIRDLVDRGAQGVVMEVSSHALEQRRVDGCRFDVGVFTNLTRDHLDYHRDMESYFESKARLFTELLAADDTKPRRAAVI
NVDDPYGARLASDAVAPVVSYGLSPSVMVRAEMVTFSVDGISGTLVTPLGTIPFHSRLLGRFNLYNILAAVAAGVALGLS
PDAIRGGIEGDVRVPGRLERVDNDHGITVLVDYAHTGDALENVLKTVAEIATGRIITVFGCGGDRDRGKRPVMGEIAGRF
SDLSIVTSDNPRTEDAGAIIGEILGGIRSLGLREYGAEELITGFDAKGFAAVESRREAIRLAARVARPGDVLLLAGKGHE
DYQIIGTMKHHFDDREEVAAAFRELSSKGQG
>Mature_511_residues
MRLEDLARVINPSEAGGSLALEIEGLYCDSRQVRSGGLFFALRGVATDGHDYIVASRDRGAVAVVVEDPSRVPEGMTWLK
VDDARLAMSRAAAAFYGNPTDGIPVVGITGTNGKTTTTYLVEAVMARAGIPVAVLGTISYRFGDKTIPAPHTTPESVDLQ
RTIRDLVDRGAQGVVMEVSSHALEQRRVDGCRFDVGVFTNLTRDHLDYHRDMESYFESKARLFTELLAADDTKPRRAAVI
NVDDPYGARLASDAVAPVVSYGLSPSVMVRAEMVTFSVDGISGTLVTPLGTIPFHSRLLGRFNLYNILAAVAAGVALGLS
PDAIRGGIEGDVRVPGRLERVDNDHGITVLVDYAHTGDALENVLKTVAEIATGRIITVFGCGGDRDRGKRPVMGEIAGRF
SDLSIVTSDNPRTEDAGAIIGEILGGIRSLGLREYGAEELITGFDAKGFAAVESRREAIRLAARVARPGDVLLLAGKGHE
DYQIIGTMKHHFDDREEVAAAFRELSSKGQG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=486, Percent_Identity=39.3004115226337, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1786274, Length=384, Percent_Identity=25, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_GEOMG (Q39YM4)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383375.1
- ProteinModelPortal:   Q39YM4
- SMR:   Q39YM4
- STRING:   Q39YM4
- GeneID:   3738162
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0407
- NMPDR:   fig|269799.3.peg.435
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- PhylomeDB:   Q39YM4
- ProtClustDB:   PRK00139
- BioCyc:   GMET269799:GMET_0407-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54734; Mature: 54734

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 179-179 BINDING 185-185 BINDING 187-187 BINDING 385-385 BINDING 476-476 BINDING 480-480

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEDLARVINPSEAGGSLALEIEGLYCDSRQVRSGGLFFALRGVATDGHDYIVASRDRG
CCHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEECCCC
AVAVVVEDPSRVPEGMTWLKVDDARLAMSRAAAAFYGNPTDGIPVVGITGTNGKTTTTYL
EEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
VEAVMARAGIPVAVLGTISYRFGDKTIPAPHTTPESVDLQRTIRDLVDRGAQGVVMEVSS
HHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHH
HALEQRRVDGCRFDVGVFTNLTRDHLDYHRDMESYFESKARLFTELLAADDTKPRRAAVI
HHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NVDDPYGARLASDAVAPVVSYGLSPSVMVRAEMVTFSVDGISGTLVTPLGTIPFHSRLLG
ECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEECCCCCCCHHHHHHH
RFNLYNILAAVAAGVALGLSPDAIRGGIEGDVRVPGRLERVDNDHGITVLVDYAHTGDAL
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEECCCHHHCCCCCCCEEEEEEECCCCHHH
ENVLKTVAEIATGRIITVFGCGGDRDRGKRPVMGEIAGRFSDLSIVTSDNPRTEDAGAII
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHH
GEILGGIRSLGLREYGAEELITGFDAKGFAAVESRREAIRLAARVARPGDVLLLAGKGHE
HHHHHHHHHCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
DYQIIGTMKHHFDDREEVAAAFRELSSKGQG
CEEEEEEHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLEDLARVINPSEAGGSLALEIEGLYCDSRQVRSGGLFFALRGVATDGHDYIVASRDRG
CCHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEECCCC
AVAVVVEDPSRVPEGMTWLKVDDARLAMSRAAAAFYGNPTDGIPVVGITGTNGKTTTTYL
EEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
VEAVMARAGIPVAVLGTISYRFGDKTIPAPHTTPESVDLQRTIRDLVDRGAQGVVMEVSS
HHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHH
HALEQRRVDGCRFDVGVFTNLTRDHLDYHRDMESYFESKARLFTELLAADDTKPRRAAVI
HHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NVDDPYGARLASDAVAPVVSYGLSPSVMVRAEMVTFSVDGISGTLVTPLGTIPFHSRLLG
ECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEECCCCCCCHHHHHHH
RFNLYNILAAVAAGVALGLSPDAIRGGIEGDVRVPGRLERVDNDHGITVLVDYAHTGDAL
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEECCCHHHCCCCCCCEEEEEEECCCCHHH
ENVLKTVAEIATGRIITVFGCGGDRDRGKRPVMGEIAGRFSDLSIVTSDNPRTEDAGAII
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHH
GEILGGIRSLGLREYGAEELITGFDAKGFAAVESRREAIRLAARVARPGDVLLLAGKGHE
HHHHHHHHHCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
DYQIIGTMKHHFDDREEVAAAFRELSSKGQG
CEEEEEEHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA