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The map label for this gene is murF [H]

Identifier: 78221629

GI number: 78221629

Start: 450650

End: 452062

Strand: Direct

Name: murF [H]

Synonym: Gmet_0408

Alternate gene names: 78221629

Gene position: 450650-452062 (Clockwise)

Preceding gene: 78221628

Following gene: 78221630

Centisome position: 11.27

GC content: 63.13

Gene sequence:

>1413_bases
GTGTTATTCTCTCTGGCAGACATACGTGAGGCAACGGGAGGCACCCTCGGCGGCGAAGGGAAAACTGCCGGTGTGGAGGG
GGTGTCAACCGACTCCCGAACGGTTCAGCCCGGCGAGCTGTTCGTGCCGTTGCGAGGTGAACGGTTTGACGGCCACGATT
ACCTCGGCGCTGCATGCGATCGCGGGGTACAGGTTGTGCTTGCGGAGCACCAGTGGCTGGAGAAGAACGAACTTCCGCCC
GGTGTCTCCGCCGTCGCCGTCGACGATACCCTGGAGGCCCTTGGCGATTTGGCCTGTTTTCATCGGCAGCGCTTTGCCAT
TCCAGTGGTGGCCGTGACCGGGAGCAACGGCAAGACAACCACCAAGGAGATGCTGGCCGCCATCCTGGGGCAGACCGGCC
CCGGGCTCAAGACAGAGGGGAACCTCAACAACCTGATCGGGCTTCCCCAGATGCTTCTTCGCCTCGATAACTCCCATCGT
TGGGCTGTCCTGGAGATGGGGATGAGCGCCTTTGGCGAGATCGACCGTCTGGCTGAGATTGCCCGTCCGGATGTGGGGAT
CATCACCAACGCCTATCCAGCCCACCTGGAGACCCTGGGAAGTGTCGAAGGGGTGGCACGGGCCAAAGGAGAGCTCTTCC
TGCGGCTCCCTGCAGAGGGGTGCGCCGTGTTCAACGCCGACGATCCCCTCATCGCCCGTTGTCCCTCCCCCGACGGAGTT
CGTCGTGTTTCCTTCGGGCTCCACAACGCCGATGTCACTGCCGAGGCGGTCGAGAATCTTGGGCGCAATGGCCAGCGTTT
CATTCTCCGGCTTTCCACGGAATCGGTTCCGGTCACCCTGCGGGCCTTCGGCCGTCACAATATCGCCAATGCCCTTGCCG
CTGCCGCGGCTGCCCATGTCCTTGGTGTTCCCACCGAGACCATCGCCCGGGGTCTGGGAGAATTTTCACCCTATGCCAGG
AGGTTCAATCTGGAGGAGGTGGGGGACGTCATCCTCATCGACGACAGCTACAATGCTAACCCCGCCTCCATGGCGGCAGC
TCTCACCACTCTGCGGGAAATCCGCGCAGGACGCAGGGCGGTGGCGGTTCTTGGCGACATGCTCGAACTGGGCGAAGGGG
CCGAGGAGGCCCACCGACAGCTGGGACTTCTGGCAGCCACCTGCGTCGATCGTCTCTACGTGATGGGGGGAATGGCCGGC
ATTGTTGCTGCTGGGGCACTCGAGGGAGGGCTTGCGGACGATGCAGTTACGGTTGCCACGAGCCATGACGCCATTGTGGC
GGACCTGCGGCGAACCACCGCGGCGGGCGATTTCATTCTTGTGAAAGGATCCCGGGGATTGGCCATGGATCGGGTCGCGG
AAAAGATGCGTGAAGTCTTTGTCGCGTCCCTCGGGAAGGGGGCCTCGGCCTAA

Upstream 100 bases:

>100_bases
GAGGACTACCAGATTATCGGGACGATGAAGCACCACTTCGACGATCGGGAAGAGGTTGCTGCCGCTTTTCGGGAACTGTC
ATCCAAAGGACAGGGATAGC

Downstream 100 bases:

>100_bases
TGCTCTACCATCTCCTTTATCCGCTGGCGAGCGACTTCAGGATCTTCAACGTCTTTAAGTACCTGACGTTCAGGACCATT
TACGCCGTCATCACCGCCCT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLFSLADIREATGGTLGGEGKTAGVEGVSTDSRTVQPGELFVPLRGERFDGHDYLGAACDRGVQVVLAEHQWLEKNELPP
GVSAVAVDDTLEALGDLACFHRQRFAIPVVAVTGSNGKTTTKEMLAAILGQTGPGLKTEGNLNNLIGLPQMLLRLDNSHR
WAVLEMGMSAFGEIDRLAEIARPDVGIITNAYPAHLETLGSVEGVARAKGELFLRLPAEGCAVFNADDPLIARCPSPDGV
RRVSFGLHNADVTAEAVENLGRNGQRFILRLSTESVPVTLRAFGRHNIANALAAAAAAHVLGVPTETIARGLGEFSPYAR
RFNLEEVGDVILIDDSYNANPASMAAALTTLREIRAGRRAVAVLGDMLELGEGAEEAHRQLGLLAATCVDRLYVMGGMAG
IVAAGALEGGLADDAVTVATSHDAIVADLRRTTAAGDFILVKGSRGLAMDRVAEKMREVFVASLGKGASA

Sequences:

>Translated_470_residues
MLFSLADIREATGGTLGGEGKTAGVEGVSTDSRTVQPGELFVPLRGERFDGHDYLGAACDRGVQVVLAEHQWLEKNELPP
GVSAVAVDDTLEALGDLACFHRQRFAIPVVAVTGSNGKTTTKEMLAAILGQTGPGLKTEGNLNNLIGLPQMLLRLDNSHR
WAVLEMGMSAFGEIDRLAEIARPDVGIITNAYPAHLETLGSVEGVARAKGELFLRLPAEGCAVFNADDPLIARCPSPDGV
RRVSFGLHNADVTAEAVENLGRNGQRFILRLSTESVPVTLRAFGRHNIANALAAAAAAHVLGVPTETIARGLGEFSPYAR
RFNLEEVGDVILIDDSYNANPASMAAALTTLREIRAGRRAVAVLGDMLELGEGAEEAHRQLGLLAATCVDRLYVMGGMAG
IVAAGALEGGLADDAVTVATSHDAIVADLRRTTAAGDFILVKGSRGLAMDRVAEKMREVFVASLGKGASA
>Mature_470_residues
MLFSLADIREATGGTLGGEGKTAGVEGVSTDSRTVQPGELFVPLRGERFDGHDYLGAACDRGVQVVLAEHQWLEKNELPP
GVSAVAVDDTLEALGDLACFHRQRFAIPVVAVTGSNGKTTTKEMLAAILGQTGPGLKTEGNLNNLIGLPQMLLRLDNSHR
WAVLEMGMSAFGEIDRLAEIARPDVGIITNAYPAHLETLGSVEGVARAKGELFLRLPAEGCAVFNADDPLIARCPSPDGV
RRVSFGLHNADVTAEAVENLGRNGQRFILRLSTESVPVTLRAFGRHNIANALAAAAAAHVLGVPTETIARGLGEFSPYAR
RFNLEEVGDVILIDDSYNANPASMAAALTTLREIRAGRRAVAVLGDMLELGEGAEEAHRQLGLLAATCVDRLYVMGGMAG
IVAAGALEGGLADDAVTVATSHDAIVADLRRTTAAGDFILVKGSRGLAMDRVAEKMREVFVASLGKGASA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=460, Percent_Identity=39.5652173913043, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1786279, Length=264, Percent_Identity=32.1969696969697, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786276, Length=277, Percent_Identity=32.8519855595668, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49373; Mature: 49373

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00778 HIS_ACID_PHOSPHAT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSLADIREATGGTLGGEGKTAGVEGVSTDSRTVQPGELFVPLRGERFDGHDYLGAACD
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHC
RGVQVVLAEHQWLEKNELPPGVSAVAVDDTLEALGDLACFHRQRFAIPVVAVTGSNGKTT
CCEEEEEECHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
TKEMLAAILGQTGPGLKTEGNLNNLIGLPQMLLRLDNSHRWAVLEMGMSAFGEIDRLAEI
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHCHHHHHHHHHHHHH
ARPDVGIITNAYPAHLETLGSVEGVARAKGELFLRLPAEGCAVFNADDPLIARCPSPDGV
HCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEEECCCCCCC
RRVSFGLHNADVTAEAVENLGRNGQRFILRLSTESVPVTLRAFGRHNIANALAAAAAAHV
EEHCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHH
LGVPTETIARGLGEFSPYARRFNLEEVGDVILIDDSYNANPASMAAALTTLREIRAGRRA
HCCCHHHHHHHHHHCCHHHHHCCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCHHH
VAVLGDMLELGEGAEEAHRQLGLLAATCVDRLYVMGGMAGIVAAGALEGGLADDAVTVAT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEE
SHDAIVADLRRTTAAGDFILVKGSRGLAMDRVAEKMREVFVASLGKGASA
CCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLFSLADIREATGGTLGGEGKTAGVEGVSTDSRTVQPGELFVPLRGERFDGHDYLGAACD
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHC
RGVQVVLAEHQWLEKNELPPGVSAVAVDDTLEALGDLACFHRQRFAIPVVAVTGSNGKTT
CCEEEEEECHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
TKEMLAAILGQTGPGLKTEGNLNNLIGLPQMLLRLDNSHRWAVLEMGMSAFGEIDRLAEI
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHCHHHHHHHHHHHHH
ARPDVGIITNAYPAHLETLGSVEGVARAKGELFLRLPAEGCAVFNADDPLIARCPSPDGV
HCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEEECCCCCCC
RRVSFGLHNADVTAEAVENLGRNGQRFILRLSTESVPVTLRAFGRHNIANALAAAAAAHV
EEHCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHH
LGVPTETIARGLGEFSPYARRFNLEEVGDVILIDDSYNANPASMAAALTTLREIRAGRRA
HCCCHHHHHHHHHHCCHHHHHCCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCHHH
VAVLGDMLELGEGAEEAHRQLGLLAATCVDRLYVMGGMAGIVAAGALEGGLADDAVTVAT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEE
SHDAIVADLRRTTAAGDFILVKGSRGLAMDRVAEKMREVFVASLGKGASA
CCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]