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The map label for this gene is hisH

Identifier: 78221608

GI number: 78221608

Start: 431404

End: 432036

Strand: Direct

Name: hisH

Synonym: Gmet_0387

Alternate gene names: 78221608

Gene position: 431404-432036 (Clockwise)

Preceding gene: 78221607

Following gene: 78221609

Centisome position: 10.79

GC content: 58.45

Gene sequence:

>633_bases
ATGGCAACAATCGCAATCATCGACTACGGCATGGGTAATCTCCGCTCCGTCCAGAAGGGGTTCGAAAAGGTGGGGTTCGA
GGCGGTGGTGACCGCCGACCCGAAGGTGGTCCTGGAGGCGGAGAAGGTGGTCCTCCCCGGCGTGGGGGCGTTCCGGGACT
GTATGCGCAACCTGGAGCAGGGTGGGTTCGTGGAGCCGATTCTCACGGTGATCCAGGATGGACGCCCCTTCCTCGGCATC
TGCGTCGGGATGCAGCTCCTCTTCACCGATAGCGTGGAGTTCGGCCTTTACCAGGGGCTGAACGTCATTCCCGGCCACGT
CCTCCGCTTCCCCGAGGGGATGCGCGAAAGAGGCGAGGATCTCAAGGTCCCCCACATGGGGTGGAACCAGCTTTCCATCA
AACGTCGCCCTTCGGCTTTCTCCGATGTTGAGGATGGAGCGAACGTTTATTTCGTTCACTCGTTCTACGCAAAGCCCGAT
GAAGAGGGTGTCGTTGCCGCTACGAGCAGTTACGGTATTGATTTCTGCGCCGCCGTCTGGAAGGACAACATCGTCGCCAC
CCAGTTCCACCCCGAGAAGTCCCAGGCGGTGGGTTTAAGTATTTTAAAAAATTTCGCGCTTTCAAAAGCATAA

Upstream 100 bases:

>100_bases
GTCGAGGCCTGCTTCAAGGCCTTTGCCCGGGCCATGGACCAGGCGACCCAGGTGGATCCCCGGATCGAAGGGGTCATGTC
GACGAAGGGCAAGCTCTGAT

Downstream 100 bases:

>100_bases
AATCCACCATGGAGACACGGAGGCACGGAGTAATTCTCCTATGGGCCTTTCTCCGTGTCTCCGTGCCTCCGTGGTGAAAG
GTTTTTTTGAATGATCGTCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MATIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKVVLPGVGAFRDCMRNLEQGGFVEPILTVIQDGRPFLGI
CVGMQLLFTDSVEFGLYQGLNVIPGHVLRFPEGMRERGEDLKVPHMGWNQLSIKRRPSAFSDVEDGANVYFVHSFYAKPD
EEGVVAATSSYGIDFCAAVWKDNIVATQFHPEKSQAVGLSILKNFALSKA

Sequences:

>Translated_210_residues
MATIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKVVLPGVGAFRDCMRNLEQGGFVEPILTVIQDGRPFLGI
CVGMQLLFTDSVEFGLYQGLNVIPGHVLRFPEGMRERGEDLKVPHMGWNQLSIKRRPSAFSDVEDGANVYFVHSFYAKPD
EEGVVAATSSYGIDFCAAVWKDNIVATQFHPEKSQAVGLSILKNFALSKA
>Mature_209_residues
ATIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKVVLPGVGAFRDCMRNLEQGGFVEPILTVIQDGRPFLGIC
VGMQLLFTDSVEFGLYQGLNVIPGHVLRFPEGMRERGEDLKVPHMGWNQLSIKRRPSAFSDVEDGANVYFVHSFYAKPDE
EGVVAATSSYGIDFCAAVWKDNIVATQFHPEKSQAVGLSILKNFALSKA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=203, Percent_Identity=41.3793103448276, Blast_Score=131, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=37.962962962963, Blast_Score=128, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_GEOMG (Q39YP4)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383355.1
- ProteinModelPortal:   Q39YP4
- SMR:   Q39YP4
- STRING:   Q39YP4
- GeneID:   3739753
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0387
- NMPDR:   fig|269799.3.peg.399
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   Q39YP4
- ProtClustDB:   PRK13141
- BioCyc:   GMET269799:GMET_0387-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23002; Mature: 22871

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 190-190 ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKVVLPGVGAFRDCMRNLEQ
CEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECEEEECCCHHHHHHHHHHHC
GGFVEPILTVIQDGRPFLGICVGMQLLFTDSVEFGLYQGLNVIPGHVLRFPEGMRERGED
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHCCHHHHHCCCC
LKVPHMGWNQLSIKRRPSAFSDVEDGANVYFVHSFYAKPDEEGVVAATSSYGIDFCAAVW
CCCCCCCCCCEEEECCCCCCCHHCCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHH
KDNIVATQFHPEKSQAVGLSILKNFALSKA
CCCEEEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKVVLPGVGAFRDCMRNLEQ
EEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECEEEECCCHHHHHHHHHHHC
GGFVEPILTVIQDGRPFLGICVGMQLLFTDSVEFGLYQGLNVIPGHVLRFPEGMRERGED
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHCCHHHHHCCCC
LKVPHMGWNQLSIKRRPSAFSDVEDGANVYFVHSFYAKPDEEGVVAATSSYGIDFCAAVW
CCCCCCCCCCEEEECCCCCCCHHCCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHH
KDNIVATQFHPEKSQAVGLSILKNFALSKA
CCCEEEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA