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The map label for this gene is hisA

Identifier: 78221609

GI number: 78221609

Start: 432127

End: 432861

Strand: Direct

Name: hisA

Synonym: Gmet_0388

Alternate gene names: 78221609

Gene position: 432127-432861 (Clockwise)

Preceding gene: 78221608

Following gene: 78221610

Centisome position: 10.81

GC content: 65.17

Gene sequence:

>735_bases
ATGATCGTCATACCTGCCATCGACCTCAAGGAAGGAAAGTGCGTCCGCCTCGAACAGGGGCTCATGGAGAAGGACACTGT
CTTCTGCGACAATCCGGCCGACCAGGCCCGGGCGTGGGAGCGCCAGGGGGCGGAACTACTCCATATCGTTGACCTGGACG
GCGCCTTTGCCGGTGAGCCGAAAAACCGGGCCTCCATCGAAGCGATTGTCAAGGCCATTGCCATTCCGACCCAACTAGGG
GGGGGCATCCGGGACATCCCCACCATTGAGGCGTACCTATCGCTTGGCATCGGCCGGGTGATCCTCGGCACTGCGGCCCA
GCGCAACCCGGAGCTGGTGGAGGAGGCGTGCCGCCTCTTTCCCGGACGGATCGTTGTGGGGATCGACGCCAAGGACGGCA
TGGTGGCGGTCCAGGGGTGGGCCGAGGTGACCGGCGTCACGGCGGTGGATCTGGCGAAGCGGTTCGAGGGGTACGGCGTC
GCCGCAATCATCTACACCGACATCGCTCGTGACGGCATGATGCAGGGGCCCAACATCGCGGCGACCAGAGCCTTGGCCGA
GGCGATCTCGATCCCGGTCATCGCTTCCGGCGGGGTGTCGTCCCTGAAGGACATCGAGAACCTCATGACCATTGAGGCGA
GCGGCATCGCCGGCGCCATTACGGGCAAGGCAGTCTACACCGGGGCCATCAACCTGGCCGAGGCCGTGGCCCTCACGAAG
CGAGGAGGGGCGTAA

Upstream 100 bases:

>100_bases
AAAAGCATAAAATCCACCATGGAGACACGGAGGCACGGAGTAATTCTCCTATGGGCCTTTCTCCGTGTCTCCGTGCCTCC
GTGGTGAAAGGTTTTTTTGA

Downstream 100 bases:

>100_bases
CATGCTGACCAAGCGGATTATTCCGTGCCTCGACGTGAAGGGGGGGCGGGTCGTCAAGGGGGTCCAGTTCCTGGAGCTGC
GGGACGCCGGCGACCCGGTG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIVIPAIDLKEGKCVRLEQGLMEKDTVFCDNPADQARAWERQGAELLHIVDLDGAFAGEPKNRASIEAIVKAIAIPTQLG
GGIRDIPTIEAYLSLGIGRVILGTAAQRNPELVEEACRLFPGRIVVGIDAKDGMVAVQGWAEVTGVTAVDLAKRFEGYGV
AAIIYTDIARDGMMQGPNIAATRALAEAISIPVIASGGVSSLKDIENLMTIEASGIAGAITGKAVYTGAINLAEAVALTK
RGGA

Sequences:

>Translated_244_residues
MIVIPAIDLKEGKCVRLEQGLMEKDTVFCDNPADQARAWERQGAELLHIVDLDGAFAGEPKNRASIEAIVKAIAIPTQLG
GGIRDIPTIEAYLSLGIGRVILGTAAQRNPELVEEACRLFPGRIVVGIDAKDGMVAVQGWAEVTGVTAVDLAKRFEGYGV
AAIIYTDIARDGMMQGPNIAATRALAEAISIPVIASGGVSSLKDIENLMTIEASGIAGAITGKAVYTGAINLAEAVALTK
RGGA
>Mature_244_residues
MIVIPAIDLKEGKCVRLEQGLMEKDTVFCDNPADQARAWERQGAELLHIVDLDGAFAGEPKNRASIEAIVKAIAIPTQLG
GGIRDIPTIEAYLSLGIGRVILGTAAQRNPELVEEACRLFPGRIVVGIDAKDGMVAVQGWAEVTGVTAVDLAKRFEGYGV
AAIIYTDIARDGMMQGPNIAATRALAEAISIPVIASGGVSSLKDIENLMTIEASGIAGAITGKAVYTGAINLAEAVALTK
RGGA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=31.2757201646091, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=23.0452674897119, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_GEOMG (Q39YP3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383356.1
- ProteinModelPortal:   Q39YP3
- SMR:   Q39YP3
- STRING:   Q39YP3
- GeneID:   3739754
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0388
- NMPDR:   fig|269799.3.peg.400
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q39YP3
- ProtClustDB:   PRK00748
- BioCyc:   GMET269799:GMET_0388-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25422; Mature: 25422

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIPAIDLKEGKCVRLEQGLMEKDTVFCDNPADQARAWERQGAELLHIVDLDGAFAGEP
CEEECCCCCCCCCEEEHHHCCCCCCCEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
KNRASIEAIVKAIAIPTQLGGGIRDIPTIEAYLSLGIGRVILGTAAQRNPELVEEACRLF
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHC
PGRIVVGIDAKDGMVAVQGWAEVTGVTAVDLAKRFEGYGVAAIIYTDIARDGMMQGPNIA
CCEEEEEEECCCCEEEEECHHHHCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCHH
ATRALAEAISIPVIASGGVSSLKDIENLMTIEASGIAGAITGKAVYTGAINLAEAVALTK
HHHHHHHHHCCCEEECCCHHHHHHHHHHHEEECCCCEEEEECCEEEECHHHHHHHHHHHC
RGGA
CCCC
>Mature Secondary Structure
MIVIPAIDLKEGKCVRLEQGLMEKDTVFCDNPADQARAWERQGAELLHIVDLDGAFAGEP
CEEECCCCCCCCCEEEHHHCCCCCCCEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
KNRASIEAIVKAIAIPTQLGGGIRDIPTIEAYLSLGIGRVILGTAAQRNPELVEEACRLF
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHC
PGRIVVGIDAKDGMVAVQGWAEVTGVTAVDLAKRFEGYGVAAIIYTDIARDGMMQGPNIA
CCEEEEEEECCCCEEEEECHHHHCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCHH
ATRALAEAISIPVIASGGVSSLKDIENLMTIEASGIAGAITGKAVYTGAINLAEAVALTK
HHHHHHHHHCCCEEECCCHHHHHHHHHHHEEECCCCEEEEECCEEEECHHHHHHHHHHHC
RGGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA