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The map label for this gene is carB [H]

Identifier: 78189743

GI number: 78189743

Start: 2306406

End: 2308271

Strand: Reverse

Name: carB [H]

Synonym: Cag_1787

Alternate gene names: 78189743

Gene position: 2308271-2306406 (Counterclockwise)

Preceding gene: 78189746

Following gene: 78189742

Centisome position: 89.74

GC content: 46.3

Gene sequence:

>1866_bases
ATGAGCACCATTACGCCCGACCAAACGGTTCTTACCCTTGCCAAAAAATTGTCTGCCGAGCAGCTTTTTGCCGCTAAGCA
AAATGGCTTTTCTGACCTGCAGCTTGCCACTATTTTTAAAACATCCGATACCGTTATTCGTGAGCTTCGTCGCCATTATG
GCATTGCCTCCGTGTTTAAAACGGTTGATACTTGTGCGGCGGAGTTTGATGCAAAAACCCCTTATCACTATTCAACCTAT
GAAGAGGAAAATGAGTCGGTTTGCTCTGATAGGAAAAAGGTGATTATTCTGGGTGGTGGACCTAACCGCATTGGGCAAGG
TATTGAGTTTGACTACTGCTGTGTACAAGCGGTGTTTGCTTTGCGAGAAGCGGGTTACGAAACCATTATGGTTAATTGCA
ATCCCGAAACGGTTTCAACCGATTACGATATTGCCGATAAACTTTACTTTGAGCCGCTTACCTTTGAGGATACCATTCGC
ATTATTGAGCATGAAAAGCCACTTGGTGTTATTGTAAGCTTTGGCGGACAAACGCCACTCAAGCTCTCTACCCGTTTGCA
TGAAGCGGGTGTAAAAATTCTTGGCACCTCATCTAAAGGCATTGACTTAGCTGAGGATCGCAAAAAGTTTGGAGCCTTGC
TTGTTGAGCTTGGTATTCCCCATCCAGCTTACGGCACGGCTATTAGTTTGGAAGAGGCAAAAGCCATTACCCAACGTATT
GGCTATCCCGCCTTAGTTCGCCCCAGCTATGTGCTTGGCGGACGTGCTATGAAAATTGTCTATAACGACGATTCGCTGAA
GGAGTACATTGATCAAGCGCTCTTTATTTCCGAAAAATATCCGCTCTTAATTGATCGCTTTCTTGAAACTGCTGTGGAGT
TTGATATTGATGCCCTTGCCGATAGCACCGATTGTGTGATTAGCGGCATTATGCAGCATGTAGAAGCAGCGGGCATTCAC
AGTGGCGACTCCACCTCCATTCTCCCTTACCATAACATTAGCAAGCAGGCAATTGCTGCCATGAAGGAGTACACCCGAAT
GCTTGCTAAAAGCTTGAATGTTATTGGGTTAATGAATGTGCAGTACGCTGTGCAAAACGACACGGTGTATGTTATTGAGG
TGAACCCTCGTGCCAGCCGCACCGTGCCATTTGTGGGTAAAGCCACCGCTATTCCGGTGGTAAAAATTGCTACCCGCGTT
ATGCTTGGCGAAAAGCTCTGCGATTTGCGCAACGAGTACAATTTAAAGGATTGCGATGAACTTGGCATGAAGCACATGGC
AATTAAAGAGCCTGTTTTCCCCTTCTCGAAGTTTGTAAAATCGGGCGTTTATCTTGGTCCCGAAATGCGCTCTACGGGTG
AAGCTATGAGTTTAGCGAACGACTTCCCCGAAGCTTTTGCAAAAGCCTATCAAGCCGCAAATATGCAGCTTCCGCTTTCG
GGCGCAGTGTTTATTAGTGTGAACGATCAAGATAAAAACCATCGTATGCTTGCTATTGCTCGCTCGTTGTACGATATGGA
TTTTGATTTAGTGGCAACGGCTGGTACATGGCAGTTCCTTACCGATAATGGTATTGAGTGCAAAAAAGTATATAAAGTAG
GTGAAGAGGGGCGTCCCAATATTTTTGACAGCATCAAACACGGCAAAGTTGATTTTGTGATTAATACGCCACGCGGCGAA
AAAGCACTGCACGATGAAGAGGCAATTGGTGCGGCATCAGTGTTAAGCAACGTGCCATTTGTAACCACCATTGAGGCGGC
TGAAGCCTCCGTGCAAGCAATTGGCTGCATTCGCCATCAAGAGTTTGGGGTAAAGAGCTTGCAAGAGTATGCAGCGTATC
GCGACACAGCTACCGCCACCTGTTAA

Upstream 100 bases:

>100_bases
GATGCTTTTAATAGCTCATGTTCGGGCATGTTCTAACTTTGAGAAGCAGGGGCATTTTTGTATTATAGCAGCACCTTTTA
AAGCTTTCAACTATCTCTGC

Downstream 100 bases:

>100_bases
GTATTAAGATTTAATAATCACATACCATGAGTGAAACTTTCCGTCAATTGCTTGACGAATCCATACAACTTGAGCTGAAT
CTCGCCAAGCTTTATACCGC

Product: carbamoyl-phosphate synthase, large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MSTITPDQTVLTLAKKLSAEQLFAAKQNGFSDLQLATIFKTSDTVIRELRRHYGIASVFKTVDTCAAEFDAKTPYHYSTY
EEENESVCSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIR
IIEHEKPLGVIVSFGGQTPLKLSTRLHEAGVKILGTSSKGIDLAEDRKKFGALLVELGIPHPAYGTAISLEEAKAITQRI
GYPALVRPSYVLGGRAMKIVYNDDSLKEYIDQALFISEKYPLLIDRFLETAVEFDIDALADSTDCVISGIMQHVEAAGIH
SGDSTSILPYHNISKQAIAAMKEYTRMLAKSLNVIGLMNVQYAVQNDTVYVIEVNPRASRTVPFVGKATAIPVVKIATRV
MLGEKLCDLRNEYNLKDCDELGMKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLANDFPEAFAKAYQAANMQLPLS
GAVFISVNDQDKNHRMLAIARSLYDMDFDLVATAGTWQFLTDNGIECKKVYKVGEEGRPNIFDSIKHGKVDFVINTPRGE
KALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIGCIRHQEFGVKSLQEYAAYRDTATATC

Sequences:

>Translated_621_residues
MSTITPDQTVLTLAKKLSAEQLFAAKQNGFSDLQLATIFKTSDTVIRELRRHYGIASVFKTVDTCAAEFDAKTPYHYSTY
EEENESVCSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIR
IIEHEKPLGVIVSFGGQTPLKLSTRLHEAGVKILGTSSKGIDLAEDRKKFGALLVELGIPHPAYGTAISLEEAKAITQRI
GYPALVRPSYVLGGRAMKIVYNDDSLKEYIDQALFISEKYPLLIDRFLETAVEFDIDALADSTDCVISGIMQHVEAAGIH
SGDSTSILPYHNISKQAIAAMKEYTRMLAKSLNVIGLMNVQYAVQNDTVYVIEVNPRASRTVPFVGKATAIPVVKIATRV
MLGEKLCDLRNEYNLKDCDELGMKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLANDFPEAFAKAYQAANMQLPLS
GAVFISVNDQDKNHRMLAIARSLYDMDFDLVATAGTWQFLTDNGIECKKVYKVGEEGRPNIFDSIKHGKVDFVINTPRGE
KALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIGCIRHQEFGVKSLQEYAAYRDTATATC
>Mature_620_residues
STITPDQTVLTLAKKLSAEQLFAAKQNGFSDLQLATIFKTSDTVIRELRRHYGIASVFKTVDTCAAEFDAKTPYHYSTYE
EENESVCSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRI
IEHEKPLGVIVSFGGQTPLKLSTRLHEAGVKILGTSSKGIDLAEDRKKFGALLVELGIPHPAYGTAISLEEAKAITQRIG
YPALVRPSYVLGGRAMKIVYNDDSLKEYIDQALFISEKYPLLIDRFLETAVEFDIDALADSTDCVISGIMQHVEAAGIHS
GDSTSILPYHNISKQAIAAMKEYTRMLAKSLNVIGLMNVQYAVQNDTVYVIEVNPRASRTVPFVGKATAIPVVKIATRVM
LGEKLCDLRNEYNLKDCDELGMKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLANDFPEAFAKAYQAANMQLPLSG
AVFISVNDQDKNHRMLAIARSLYDMDFDLVATAGTWQFLTDNGIECKKVYKVGEEGRPNIFDSIKHGKVDFVINTPRGEK
ALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIGCIRHQEFGVKSLQEYAAYRDTATATC

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI170295797, Length=607, Percent_Identity=36.7380560131796, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI169790915, Length=607, Percent_Identity=36.7380560131796, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI21361331, Length=607, Percent_Identity=36.7380560131796, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI18105007, Length=549, Percent_Identity=36.6120218579235, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1786216, Length=579, Percent_Identity=49.2227979274611, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI193204318, Length=596, Percent_Identity=36.0738255033557, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6322331, Length=591, Percent_Identity=36.3790186125212, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322569, Length=481, Percent_Identity=39.0852390852391, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24642586, Length=560, Percent_Identity=36.7857142857143, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI45555749, Length=520, Percent_Identity=37.8846153846154, Blast_Score=352, Evalue=5e-97,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 68440; Mature: 68309

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50975 ATP_GRASP ; PS00962 RIBOSOMAL_S2_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTITPDQTVLTLAKKLSAEQLFAAKQNGFSDLQLATIFKTSDTVIRELRRHYGIASVFK
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCHHHHHH
TVDTCAAEFDAKTPYHYSTYEEENESVCSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFA
HHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHH
LREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEKPLGVIVSFGGQTPL
HHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHHHHHCCCCCCEEEEEECCCCCH
KLSTRLHEAGVKILGTSSKGIDLAEDRKKFGALLVELGIPHPAYGTAISLEEAKAITQRI
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEHHHHHHHHHHC
GYPALVRPSYVLGGRAMKIVYNDDSLKEYIDQALFISEKYPLLIDRFLETAVEFDIDALA
CCCCCCCCCEEECCEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHC
DSTDCVISGIMQHVEAAGIHSGDSTSILPYHNISKQAIAAMKEYTRMLAKSLNVIGLMNV
CCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEE
QYAVQNDTVYVIEVNPRASRTVPFVGKATAIPVVKIATRVMLGEKLCDLRNEYNLKDCDE
EEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LGMKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLANDFPEAFAKAYQAANMQLPLS
HCCHHHHHCCCCCHHHHHHHCCCEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCC
GAVFISVNDQDKNHRMLAIARSLYDMDFDLVATAGTWQFLTDNGIECKKVYKVGEEGRPN
CEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCHHHHHHCCCCCCCC
IFDSIKHGKVDFVINTPRGEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIGCIRHQ
HHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHH
EFGVKSLQEYAAYRDTATATC
HCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STITPDQTVLTLAKKLSAEQLFAAKQNGFSDLQLATIFKTSDTVIRELRRHYGIASVFK
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCHHHHHH
TVDTCAAEFDAKTPYHYSTYEEENESVCSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFA
HHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHH
LREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEKPLGVIVSFGGQTPL
HHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHHHHHCCCCCCEEEEEECCCCCH
KLSTRLHEAGVKILGTSSKGIDLAEDRKKFGALLVELGIPHPAYGTAISLEEAKAITQRI
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEHHHHHHHHHHC
GYPALVRPSYVLGGRAMKIVYNDDSLKEYIDQALFISEKYPLLIDRFLETAVEFDIDALA
CCCCCCCCCEEECCEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHC
DSTDCVISGIMQHVEAAGIHSGDSTSILPYHNISKQAIAAMKEYTRMLAKSLNVIGLMNV
CCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEE
QYAVQNDTVYVIEVNPRASRTVPFVGKATAIPVVKIATRVMLGEKLCDLRNEYNLKDCDE
EEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LGMKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLANDFPEAFAKAYQAANMQLPLS
HCCHHHHHCCCCCHHHHHHHCCCEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCC
GAVFISVNDQDKNHRMLAIARSLYDMDFDLVATAGTWQFLTDNGIECKKVYKVGEEGRPN
CEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCHHHHHHCCCCCCCC
IFDSIKHGKVDFVINTPRGEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIGCIRHQ
HHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHH
EFGVKSLQEYAAYRDTATATC
HCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]