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The map label for this gene is pleD [H]

Identifier: 78188889

GI number: 78188889

Start: 1253056

End: 1254312

Strand: Reverse

Name: pleD [H]

Synonym: Cag_0921

Alternate gene names: 78188889

Gene position: 1254312-1253056 (Counterclockwise)

Preceding gene: 78188900

Following gene: 78188888

Centisome position: 48.77

GC content: 46.54

Gene sequence:

>1257_bases
ATGACTCTCTTTCTTCAACGGCTAACAAATGCCGTTAAACATATATCACCCTTACAAGCACTTGCCGTAGCAGGCGTGGT
TGGTATGGCGTTGGTGGTAGCGGGATCGGTGTTTTTTGTTACAACACAGCATCGTTTGCTTCGGGAGATGGAGCATCGGC
AAGCGTTGTATTTAGCATTGGTTGAATTTCGTAGCACTCTGCTTGCATGCGAACATTCAATCGGTGATGAGAGTTTGCCA
TCTCACTGCACGCCAACAGCGTTGCAACAATTATTGCCGTTGATGCGTGCGGCTCATGCGGAAGCTCTACCGCACTTACA
GCAGCTTGCGTTATCCAAGCGAACGTTTACCAAGAAAGAGATTGCTACATTAAAAACCGAGCTTGAAAGAGCAATTCCTT
TAGCACAGCAAAAAGCAGCTCAAGCATCGGTGGATTTATTGGGGGTTAATCAACGCTTTATGTTGGCTTTAGTGCTACTC
TTACTCTTGCTTGGCTTAACGGCAGGCTTAGTGCTTCGCCATAATTATCGCCAAACAATCATCCCATTAGCCCAATTAGT
GGCACAGCTTAAACTCCTTAACCGCAACATTCCCGAAAGCATTCGCGATACGGCTGCCGAAATGAGCCGTGAATTACGTG
GCACGGCGGCACATTCGCACGATATTACCCAAATTACCGAGTCAGTAATTCGGTTGTGTGGCGATATTGAGGCTAAAAAT
CGTAAGCTGGATGAACTTTACATTCGCGATGAAAAAACGCAACTCTTTAATTATCGACATTTTAAAGAGCATTTGATTCT
TGATATTGAGCGGGCAAAGCGGCAACTCGACTCTTTATCGCTGGCTATGTTGGATATTGATTTTTTCAAGCAATACAACG
ATGCCAATGGTCATCTTTCGGGTGATAAAGCACTGAAGCGTTTTGCCGAGCTCATTAGCGCTGAATGCCGTGTCTATGAT
TTACCTGCCCGTTTTGGGGGCGACGAATTCGCTCTTCTTTTTCCCCGCACAAGCGCCGCTGATGCAGCCGATATTGCCGA
GCGCCTTCGCCATCTTATTGAATCAGCCTCATTTCCTCATGCGGAGTGCTTTCCCGATGGAAAACTCACCATCAGCATTG
GTCTTGCAACCTTCCCAAACGATGCGCAAGATTGGCATTCACTCCTTAACAATGCCGATAGGGCGCTCTATAAAGCCAAA
GCACTGGGACGTAACAGCGTGGTGAGTTTTAGTGAAGTGAAACAAGGCATTGCTTAA

Upstream 100 bases:

>100_bases
ATAAACGCTCTACCATTGTTTATATTGATGCTCTTTGTTGCTTGTATGTTGAGCGTTAAGCGCAAAAACAAAGCATTCAT
TTTGCTGTATCATTGGTACT

Downstream 100 bases:

>100_bases
TCTGAATAACGAGTAAAAAACGATGTTCTTTCAGAGAAAACAAGAAATTCAAACATTAACGAGGAGCTTTTGAACAATCC
CGAAGGGAATTGAATATGAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTLFLQRLTNAVKHISPLQALAVAGVVGMALVVAGSVFFVTTQHRLLREMEHRQALYLALVEFRSTLLACEHSIGDESLP
SHCTPTALQQLLPLMRAAHAEALPHLQQLALSKRTFTKKEIATLKTELERAIPLAQQKAAQASVDLLGVNQRFMLALVLL
LLLLGLTAGLVLRHNYRQTIIPLAQLVAQLKLLNRNIPESIRDTAAEMSRELRGTAAHSHDITQITESVIRLCGDIEAKN
RKLDELYIRDEKTQLFNYRHFKEHLILDIERAKRQLDSLSLAMLDIDFFKQYNDANGHLSGDKALKRFAELISAECRVYD
LPARFGGDEFALLFPRTSAADAADIAERLRHLIESASFPHAECFPDGKLTISIGLATFPNDAQDWHSLLNNADRALYKAK
ALGRNSVVSFSEVKQGIA

Sequences:

>Translated_418_residues
MTLFLQRLTNAVKHISPLQALAVAGVVGMALVVAGSVFFVTTQHRLLREMEHRQALYLALVEFRSTLLACEHSIGDESLP
SHCTPTALQQLLPLMRAAHAEALPHLQQLALSKRTFTKKEIATLKTELERAIPLAQQKAAQASVDLLGVNQRFMLALVLL
LLLLGLTAGLVLRHNYRQTIIPLAQLVAQLKLLNRNIPESIRDTAAEMSRELRGTAAHSHDITQITESVIRLCGDIEAKN
RKLDELYIRDEKTQLFNYRHFKEHLILDIERAKRQLDSLSLAMLDIDFFKQYNDANGHLSGDKALKRFAELISAECRVYD
LPARFGGDEFALLFPRTSAADAADIAERLRHLIESASFPHAECFPDGKLTISIGLATFPNDAQDWHSLLNNADRALYKAK
ALGRNSVVSFSEVKQGIA
>Mature_417_residues
TLFLQRLTNAVKHISPLQALAVAGVVGMALVVAGSVFFVTTQHRLLREMEHRQALYLALVEFRSTLLACEHSIGDESLPS
HCTPTALQQLLPLMRAAHAEALPHLQQLALSKRTFTKKEIATLKTELERAIPLAQQKAAQASVDLLGVNQRFMLALVLLL
LLLGLTAGLVLRHNYRQTIIPLAQLVAQLKLLNRNIPESIRDTAAEMSRELRGTAAHSHDITQITESVIRLCGDIEAKNR
KLDELYIRDEKTQLFNYRHFKEHLILDIERAKRQLDSLSLAMLDIDFFKQYNDANGHLSGDKALKRFAELISAECRVYDL
PARFGGDEFALLFPRTSAADAADIAERLRHLIESASFPHAECFPDGKLTISIGLATFPNDAQDWHSLLNNADRALYKAKA
LGRNSVVSFSEVKQGIA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=204, Percent_Identity=34.3137254901961, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=33.9393939393939, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693134, Length=155, Percent_Identity=36.1290322580645, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=32.7683615819209, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=36.1538461538462, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787816, Length=136, Percent_Identity=32.3529411764706, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=31.5151515151515, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081881, Length=131, Percent_Identity=38.9312977099237, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=29.2682926829268, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=136, Percent_Identity=33.0882352941176, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46618; Mature: 46486

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFLQRLTNAVKHISPLQALAVAGVVGMALVVAGSVFFVTTQHRLLREMEHRQALYLAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHHHHHHHHH
VEFRSTLLACEHSIGDESLPSHCTPTALQQLLPLMRAAHAEALPHLQQLALSKRTFTKKE
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IATLKTELERAIPLAQQKAAQASVDLLGVNQRFMLALVLLLLLLGLTAGLVLRHNYRQTI
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IPLAQLVAQLKLLNRNIPESIRDTAAEMSRELRGTAAHSHDITQITESVIRLCGDIEAKN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
RKLDELYIRDEKTQLFNYRHFKEHLILDIERAKRQLDSLSLAMLDIDFFKQYNDANGHLS
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDKALKRFAELISAECRVYDLPARFGGDEFALLFPRTSAADAADIAERLRHLIESASFPH
HHHHHHHHHHHHHCCCEEEECCHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
AECFPDGKLTISIGLATFPNDAQDWHSLLNNADRALYKAKALGRNSVVSFSEVKQGIA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCC
>Mature Secondary Structure 
TLFLQRLTNAVKHISPLQALAVAGVVGMALVVAGSVFFVTTQHRLLREMEHRQALYLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHHHHHHHHH
VEFRSTLLACEHSIGDESLPSHCTPTALQQLLPLMRAAHAEALPHLQQLALSKRTFTKKE
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IATLKTELERAIPLAQQKAAQASVDLLGVNQRFMLALVLLLLLLGLTAGLVLRHNYRQTI
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IPLAQLVAQLKLLNRNIPESIRDTAAEMSRELRGTAAHSHDITQITESVIRLCGDIEAKN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
RKLDELYIRDEKTQLFNYRHFKEHLILDIERAKRQLDSLSLAMLDIDFFKQYNDANGHLS
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDKALKRFAELISAECRVYDLPARFGGDEFALLFPRTSAADAADIAERLRHLIESASFPH
HHHHHHHHHHHHHCCCEEEECCHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
AECFPDGKLTISIGLATFPNDAQDWHSLLNNADRALYKAKALGRNSVVSFSEVKQGIA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA