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The map label for this gene is metK [H]

Identifier: 78067900

GI number: 78067900

Start: 3597972

End: 3599159

Strand: Reverse

Name: metK [H]

Synonym: Bcep18194_A6431

Alternate gene names: 78067900

Gene position: 3599159-3597972 (Counterclockwise)

Preceding gene: 78067902

Following gene: 78067898

Centisome position: 97.43

GC content: 65.15

Gene sequence:

>1188_bases
GTGGCAAACGATTATCTCTTTACGTCCGAATCCGTCTCTGAAGGCCATCCGGACAAAGTCGCGGACCAAATCTCGGACGC
GATCCTCGACGCCATCCTCGAGCAGGACAAGTATTCCCGCGTTGCGGCGGAAACGCTGTGCAACACGGGTCTTGTCGTGC
TGGCCGGTGAAATCACCACGACGGCCAACATCGACTACATCCAGATCGCGCGCGACACGATCAAGCGCATCGGCTACGAC
AACACCGACTACGGCATCGACTACAAGGGTTGTGCCGTGCTCGTCGCGTACGACAAGCAGTCGCCGGACATCGCCCAGGG
CGTCGATCGTGCACACGACGACAACCTCGACCAAGGCGCGGGCGACCAGGGTCTGATGTTCGGTTACGCGTGCGATGAAA
CGCCGGAACTGATGCCGCTGCCGATCTACCTGTCGCACCGCCTCGTCGAGCGCCAGGCCAGCCTGCGCCGCGACGGCCGC
CTGCAATGGCTGCGCCCGGACGCGAAGTCGCAGGTGACGGTCCGCTACGTCGACGGCAAGCCGCATTCGATCGACACCGT
CGTGCTGTCGACGCAGCACGCACCGGACATCGAGCTGCCGGCGCTGCGCGAAGCCGTGATCGAGGAAATCATCAAGCCGA
CGCTGCCGGCCGACCTGATCAAGGGTGACATCAAGTTCCTGGTGAACCCGACCGGCCGGTTCGTGATCGGCGGCCCGCAG
GGCGACTGCGGCCTGACCGGCCGCAAGATCATCGTCGATACGTACGGCGGTGCCGCACCGCACGGCGGCGGCGCGTTCTC
GGGCAAGGATCCGTCGAAGGTCGACCGTTCGGCTGCATACGCAGGCCGCTACGTCGCGAAGAACATCGTCGCCGCCGGCC
TCGCGTCGCGCGCGCTGATCCAGGTGTCGTACGCGATCGGCGTTGCCGAGCCGACCTCAGTGATGGTCAACACGTTCGGC
ACGGGCCGCGTGTCGGATGCGGTGATCACGAAGCTCGTGCGCGAGCATTTCGACCTGCGTCCGAAGGGCATCATCAAGAT
GCTCGACCTGCTGCGCCCGATCTACGAGAAGACCGCTGCTTACGGCCACTTCGGCCGCGAAGAGCCGGAATTCTCGTGGG
AAGCGACCGACAAGGCGCTCGCCCTGGCCGAAGCGGCCGGCGTCGAGCCGACGGCACGCGTCGCGTAA

Upstream 100 bases:

>100_bases
CGCGCTGCCCGGTCCTGGTAATCCGCTAAAGCGTCGCCGCTTCAGGCCCAACGAAGCTGGCTACGTGAAACCAACCGTAA
CCACACAGGAGCCTGAAAAA

Downstream 100 bases:

>100_bases
GCGTCGTTCGCTGCAAATGAAAAACCCCGGCAAAGCCGGGGTTTTTTATGGGTTCCGATCGTCGTGGCAATCGGACCGGC
GGGCGGCAGCGCGACGGCGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGR
LQWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA

Sequences:

>Translated_395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGR
LQWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
>Mature_394_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDN
TDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRL
QWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGT
GRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=378, Percent_Identity=58.2010582010582, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=56.3492063492063, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=69.6103896103896, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.6842105263158, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.5106382978723, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=58.7765957446808, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=55.7840616966581, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=53.1914893617021, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=57.2289156626506, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.6219512195122, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=57.8073089700997, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=57.1428571428571, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=59.1029023746702, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=59.2592592592593, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.0531914893617, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=54.5454545454545, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=54.5454545454545, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52.2666666666667, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=54.6218487394958, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42717; Mature: 42586

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRLQWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCC
PHSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRLQWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCC
PHSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA