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The map label for this gene is puuA [H]

Identifier: 78067812

GI number: 78067812

Start: 3505937

End: 3507274

Strand: Direct

Name: puuA [H]

Synonym: Bcep18194_A6343

Alternate gene names: 78067812

Gene position: 3505937-3507274 (Clockwise)

Preceding gene: 78067811

Following gene: 78067813

Centisome position: 94.91

GC content: 65.02

Gene sequence:

>1338_bases
ATGCATGACATCGAAGATTTTCTGAAGCAGCACCGGATCACTGAAGTCGAAGCGATCATTCCCGACATGGCCGGCATCGC
GCGCGGCAAGATCACGCCGCGCAACAAGTTCACTTCCGGCGAATCGATGCGCCTGCCGCAGGCCGTGATGGTGCAGACCG
TCACCGGCGAATACCCGGAAGACGGCTCGCTGACCGGCGTGACCGACCCCGACATGGTGTGCGTGCCCGACGCATCGACG
ATCCGCCTGATCCCGTGGGCCATCGACCCGACCGCGCAGGTCATCCACGACTGCGTGCACTTCGACGGCTCGCCGGTCGA
GATCTCGCCGCGCTACGTGCTGCGCCGCGTGCTCGACCTGTACAAGGCGAAGGGCTGGAAGCCTGTCGTCGCGCCCGAGC
TCGAGTTCTACCTGGTCGACATGAACAAGGATCCCGACCTGCCGCTGCGCCCGCCGGTCGGCCGTACGGGCCGCCCCGAA
ACGGGCCGCCAGTCGTATTCGATCGAGGCCGTCAACGAGTTCGACCCGCTGTTCGAGGACATCTACGAGTACTGCGAAGC
GCAGAACCTCGACATCGACACGCTGATCCACGAAGTCGGCGCCGCGCAGATGGAGATCAACTTCATGCACGGCGACGCGC
TGTCGCTGGCCGACCAGGTGTTCCTGTTCAAGCGCACGGTGCGTGAGGCCGCGCTGCGTCACAACATGTACGCGACGTTC
ATGGCCAAGCCGATGGAAGACGAACCGGGTTCGGCGATGCACGTGCACCAGAGCATCGTCGACGAGGAAACGGGCCAGAA
CCTGTTCACGTCGCAGGAAACCGGCGGCGCGACGTCGATGTTCTACAACTACCTCGCGGGGCTGCAGAAGTACACGCCGG
CGCTGATGCCGATCTTCGCGCCGTACATCAACTCGTATCGCCGCCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAG
TGGGGCTACGACAACCGCACGGTCGGCTTCCGCATCCCGCACTCGGGCCCGTCGGCACGCCGCATCGAGAACCGCATTCC
GGGCGTCGACTGCAATCCGTACCTCGCGCTCGCCGCGACGCTCGCGGCCGGCTATCTCGGCATGACCCAGCGCCTGGAGC
CGACCGAGCCGCTCGTCAGCGACGGCTACGACCTGCCGTACCAGCTGCCGCGCAACCTCGAGGAAGGGCTGACGCTGATG
GCCGCCTGCGAGCCGCTCGGCGAGATCCTCGGCCACAAGTTCCTGAAGGCGTATTTCGCGCTGAAGGAAACCGAATACGA
AGCGTTCTTCCGCGTGATCAGCTCGTGGGAACGCCGCCATCTGCTGCTGCACGTCTGA

Upstream 100 bases:

>100_bases
CGCCTTCGGCGATGCCTGCCGCGCCCGCCGCGCCACCCGCACGCGTGCCACGGCCGCTGCCGCGCTCGCCTGAGCGCCGA
CCCAACCAACGAGAACAGAC

Downstream 100 bases:

>100_bases
GCGTGCACCCAACCGAATACGGAATCACGGAGGAAACATGACGTACCGCAACGAATCTGCCTGGATCCAGCCGGCCGCAC
CGAGCGCCACGGCCGCCGCA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MHDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDAST
IRLIPWAIDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSQETGGATSMFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPSARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLM
AACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_445_residues
MHDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDAST
IRLIPWAIDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSQETGGATSMFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPSARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLM
AACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV
>Mature_445_residues
MHDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDAST
IRLIPWAIDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSQETGGATSMFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPSARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLM
AACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=447, Percent_Identity=27.2930648769575, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=33.9246119733925, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790301, Length=439, Percent_Identity=28.0182232346241, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50239; Mature: 50239

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DGSLTGVTDPDMVCVPDASTIRLIPWAIDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCCCCCCCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSQETGGATSMFYNYLAGLQKYTPALMPIFA
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPSARRIENRIPGVDCNPYLALAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLMAACEPLGEILGHKFLKAYFA
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MHDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DGSLTGVTDPDMVCVPDASTIRLIPWAIDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCCCCCCCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSQETGGATSMFYNYLAGLQKYTPALMPIFA
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPSARRIENRIPGVDCNPYLALAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLMAACEPLGEILGHKFLKAYFA
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]