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The map label for this gene is yusZ [H]

Identifier: 78067281

GI number: 78067281

Start: 2928529

End: 2929428

Strand: Direct

Name: yusZ [H]

Synonym: Bcep18194_A5812

Alternate gene names: 78067281

Gene position: 2928529-2929428 (Clockwise)

Preceding gene: 78067280

Following gene: 78067283

Centisome position: 79.28

GC content: 72.22

Gene sequence:

>900_bases
ATGAAGGAAGTCATTCTCGTCACGGGCGCGTCCAGCGGCTTCGGCCTGCTGTCGGCCCAGGCGCTCGCCCGCGCCGGCCA
TACCGTCTACGCGTCGATGCGCGAAAGCGCCGGCCGCAACGCGCCGCGCGTCGCCGCCATCGCCGCCTACGCGCAGGAAC
ACGGCGTCGACCTGCGCACCGTCGAACTCGACGTCGGCGACGACGCGTCCGTCGGCGCCGCCATCGATCGCGTGATCGCG
GACAACGGCCGCCTCGACGTCATCGTCCACAACGCCGGCCACATGGTGTTCGGCCCGGCCGAAGCGTTCACGGCCGAGCA
GATCGCCCAGCTCTACGACATCAACGTCGTGTCGACCCAGCGCGTGAACCGCGCGGCGCTGCCGCACCTGCGCCGCCAGG
GCCGCGGCCTGCTCGTGTGGGTGTCGTCGTCGTCCGCGCGCGGCGGTACGCCGCCGTTCCTCGCCCCCTACTTCGCCGCG
AAGGCCGCGATGGATTCGCTCGCGGTGTCCTATGCGGCCGAACTCGCCCGCTGGGGGATCGAGACGTCGATCGTCGTGCC
GGGCGCCTTCACCCAGGGCACGAATCACTTTGCCCACGCAGGCAAGCCGGCCGACGCGGCCGTTCAGGCTGCCTACGTCG
ACGGCCCCTATGCGGGCGTCGCCGAGCAGGCGCTTCAAGGCCTCGCCGCGCTCGAACCGGCGGACGCCGATGCCGGCACC
GTCGCGACCGCGATCGCCGACATCGTCGCCGCCCCCGCCGGCAAGCGCCCGTACCGCGTGTTCGTCGATCCGTCGCAGGA
CGGCGCGGAGGAAGTGTTCCGCGTCGGCGATCGCATCCGCCGCGAGATGTTCAGGAACATCGGCCTCGCCGACCTGCTCG
CGGCCCGCGTCGGCGGCTGA

Upstream 100 bases:

>100_bases
GGCCCCGACGGCGCGTGGGTCAACGGTCAGATCCTGCGCGCCAACGGCGGCATGTGCTGACCGGCCGCCCGTCACGCCCC
CCTCTTACCGGAGATCGATC

Downstream 100 bases:

>100_bases
TCCACCGTGCGCCGGCCACGCCGGCGCGCATCAGCCTTACGCGCAGAATCCGAGGATCGCGTCGGCGACCGCCGCGGGCG
CCTCGCGCTGCGGAAAGTGG

Product: Short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: ORFA [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKEVILVTGASSGFGLLSAQALARAGHTVYASMRESAGRNAPRVAAIAAYAQEHGVDLRTVELDVGDDASVGAAIDRVIA
DNGRLDVIVHNAGHMVFGPAEAFTAEQIAQLYDINVVSTQRVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAA
KAAMDSLAVSYAAELARWGIETSIVVPGAFTQGTNHFAHAGKPADAAVQAAYVDGPYAGVAEQALQGLAALEPADADAGT
VATAIADIVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAARVGG

Sequences:

>Translated_299_residues
MKEVILVTGASSGFGLLSAQALARAGHTVYASMRESAGRNAPRVAAIAAYAQEHGVDLRTVELDVGDDASVGAAIDRVIA
DNGRLDVIVHNAGHMVFGPAEAFTAEQIAQLYDINVVSTQRVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAA
KAAMDSLAVSYAAELARWGIETSIVVPGAFTQGTNHFAHAGKPADAAVQAAYVDGPYAGVAEQALQGLAALEPADADAGT
VATAIADIVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAARVGG
>Mature_299_residues
MKEVILVTGASSGFGLLSAQALARAGHTVYASMRESAGRNAPRVAAIAAYAQEHGVDLRTVELDVGDDASVGAAIDRVIA
DNGRLDVIVHNAGHMVFGPAEAFTAEQIAQLYDINVVSTQRVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAA
KAAMDSLAVSYAAELARWGIETSIVVPGAFTQGTNHFAHAGKPADAAVQAAYVDGPYAGVAEQALQGLAALEPADADAGT
VATAIADIVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAARVGG

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI223718074, Length=192, Percent_Identity=37.5, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI7657478, Length=187, Percent_Identity=32.0855614973262, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI119392083, Length=190, Percent_Identity=29.4736842105263, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI44680136, Length=201, Percent_Identity=28.3582089552239, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI44680133, Length=201, Percent_Identity=28.3582089552239, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI17738292, Length=201, Percent_Identity=28.3582089552239, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786812, Length=186, Percent_Identity=31.7204301075269, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786701, Length=227, Percent_Identity=30.8370044052863, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788459, Length=199, Percent_Identity=29.6482412060301, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87082100, Length=202, Percent_Identity=30.1980198019802, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787335, Length=189, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=186, Percent_Identity=25.2688172043011, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI2367175, Length=205, Percent_Identity=29.7560975609756, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=217, Percent_Identity=29.9539170506912, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=186, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534660, Length=192, Percent_Identity=31.25, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=211, Percent_Identity=31.2796208530806, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=198, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=188, Percent_Identity=30.8510638297872, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=198, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24651139, Length=281, Percent_Identity=27.4021352313167, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571526, Length=189, Percent_Identity=33.8624338624339, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI23397609, Length=227, Percent_Identity=31.7180616740088, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357041, Length=189, Percent_Identity=31.7460317460317, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364892, Length=195, Percent_Identity=29.7435897435897, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644339, Length=206, Percent_Identity=30.5825242718447, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 31112; Mature: 31112

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVILVTGASSGFGLLSAQALARAGHTVYASMRESAGRNAPRVAAIAAYAQEHGVDLRT
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEE
VELDVGDDASVGAAIDRVIADNGRLDVIVHNAGHMVFGPAEAFTAEQIAQLYDINVVSTQ
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCEECHH
RVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAAKAAMDSLAVSYAAELARWGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETSIVVPGAFTQGTNHFAHAGKPADAAVQAAYVDGPYAGVAEQALQGLAALEPADADAGT
CEEEEECCCCCCCCCCHHCCCCCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHH
VATAIADIVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAARVGG
HHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MKEVILVTGASSGFGLLSAQALARAGHTVYASMRESAGRNAPRVAAIAAYAQEHGVDLRT
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEE
VELDVGDDASVGAAIDRVIADNGRLDVIVHNAGHMVFGPAEAFTAEQIAQLYDINVVSTQ
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCEECHH
RVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAAKAAMDSLAVSYAAELARWGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETSIVVPGAFTQGTNHFAHAGKPADAAVQAAYVDGPYAGVAEQALQGLAALEPADADAGT
CEEEEECCCCCCCCCCHHCCCCCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHH
VATAIADIVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAARVGG
HHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 8396117 [H]