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The map label for this gene is lpxD

Identifier: 78066788

GI number: 78066788

Start: 2370538

End: 2371617

Strand: Reverse

Name: lpxD

Synonym: Bcep18194_A5319

Alternate gene names: 78066788

Gene position: 2371617-2370538 (Counterclockwise)

Preceding gene: 78066789

Following gene: 78066787

Centisome position: 64.2

GC content: 68.89

Gene sequence:

>1080_bases
ATGGCATTGACGCTTGAGGAACTCGTAAAGCGGTTCGGCGGCGAGATCGCCGGCGACGCACAGTGCAAGGTGGGCGGGCT
CGCACCGCTCGACCAGGCAGGCCCGCAGCAACTCGCGTTCCTCGCGAATCCGAAGTACCTGTCGCAGGTCGAGTCGACCC
GCGCCGGCGCGGTGCTGATCGCGCCGAAGGATCTGGAAAAGCTCGGCGCGGCCGCTGGCGGCCGCAACTTCATTGTCACG
CCGAATCCGTACGCGTACTTCGCGCGCGTCGCGCAGATGTTCATCGACCTGGCTACGCCGCAGCGTGCCGCCGGCGTGCA
TCCGAGCGCGACGATCGACCCGGCCGCGCAGGTCGCCGCGAGCGCGGTGATCGGCCCGCACGTGACGGTCGAGGCCGGTG
CTGTGATCGAAGACGGCGTGCAGCTCGATGCGAACGTATTCGTCGGCCGCGGCACGACGATCGGCGCGGGCTCGCACCTG
TATCCGAACGCGTCGGTGTACCACGGCTGCAAGGTCGGCCCGCGCGCGATCATCCATGCGGGCGCCGTGATCGGCTCCGA
CGGCTTCGGCTTCGCGCCGGATTTCGTCGGCGACGGCGACGCGCGCACCGGCAGCTGGGTCAAGATCCCGCAGGTCGGCG
GCGTCACGATCGGGCCGGACGTCGAGATCGGCGCGAACACGACGATCGACCGCGGCGCGATGGCGGATACCGTCATCGAA
GAATGCGTGAAGATCGACAACCAGGTGCAGATCGGCCACAACTGCCGGATCGGCGCCTACACGGTGATCGCCGGCAGCGC
CGGGATCGCGGGCAGCACGACGATCGGCCGCCACTGCATGATCGGCGGTGCGGCGGGGATCGCCGGCCACGTGACGCTCG
GCGACTATGTCATCATCACCGCAAAATCGGGCGTATCGAAGTCGCTGCCGAAGGCCGGCATCTATACCAGCGCGTTCCCG
GCCGTCGACCACGGCGAATGGAACAAGAGCGCCGCGCTCGTGCGCAACCTCGACAAGCTGCGCGAGCGCATCAAGGCGCT
CGAAGCCGCGCTCGCCGCCCAGGGCGGCACGGACGCCTGA

Upstream 100 bases:

>100_bases
GTGCAGGAAGCCGTGTACGTCAGCCCGCGCATCGACATCACCGACAAGGTGCTCAAGGCGCTCGCGTCCGGCTCGACGAA
CTGAACGGAGCAGACGACGA

Downstream 100 bases:

>100_bases
GCGCATCATGCGAGACCTCGAACCGGGTACGGCCAGCGCAACCGGCCCGGTTTGCCAGACTGGGCCGCACGGCGGCCCGC
TTCAGCCTTTGCATCACCAC

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MALTLEELVKRFGGEIAGDAQCKVGGLAPLDQAGPQQLAFLANPKYLSQVESTRAGAVLIAPKDLEKLGAAAGGRNFIVT
PNPYAYFARVAQMFIDLATPQRAAGVHPSATIDPAAQVAASAVIGPHVTVEAGAVIEDGVQLDANVFVGRGTTIGAGSHL
YPNASVYHGCKVGPRAIIHAGAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVTIGPDVEIGANTTIDRGAMADTVIE
ECVKIDNQVQIGHNCRIGAYTVIAGSAGIAGSTTIGRHCMIGGAAGIAGHVTLGDYVIITAKSGVSKSLPKAGIYTSAFP
AVDHGEWNKSAALVRNLDKLRERIKALEAALAAQGGTDA

Sequences:

>Translated_359_residues
MALTLEELVKRFGGEIAGDAQCKVGGLAPLDQAGPQQLAFLANPKYLSQVESTRAGAVLIAPKDLEKLGAAAGGRNFIVT
PNPYAYFARVAQMFIDLATPQRAAGVHPSATIDPAAQVAASAVIGPHVTVEAGAVIEDGVQLDANVFVGRGTTIGAGSHL
YPNASVYHGCKVGPRAIIHAGAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVTIGPDVEIGANTTIDRGAMADTVIE
ECVKIDNQVQIGHNCRIGAYTVIAGSAGIAGSTTIGRHCMIGGAAGIAGHVTLGDYVIITAKSGVSKSLPKAGIYTSAFP
AVDHGEWNKSAALVRNLDKLRERIKALEAALAAQGGTDA
>Mature_358_residues
ALTLEELVKRFGGEIAGDAQCKVGGLAPLDQAGPQQLAFLANPKYLSQVESTRAGAVLIAPKDLEKLGAAAGGRNFIVTP
NPYAYFARVAQMFIDLATPQRAAGVHPSATIDPAAQVAASAVIGPHVTVEAGAVIEDGVQLDANVFVGRGTTIGAGSHLY
PNASVYHGCKVGPRAIIHAGAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVTIGPDVEIGANTTIDRGAMADTVIEE
CVKIDNQVQIGHNCRIGAYTVIAGSAGIAGSTTIGRHCMIGGAAGIAGHVTLGDYVIITAKSGVSKSLPKAGIYTSAFPA
VDHGEWNKSAALVRNLDKLRERIKALEAALAAQGGTDA

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=353, Percent_Identity=40.7932011331445, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36582; Mature: 36451

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLEELVKRFGGEIAGDAQCKVGGLAPLDQAGPQQLAFLANPKYLSQVESTRAGAVLI
CCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHEEEECCCHHHHHHHHCCCCEEEE
APKDLEKLGAAAGGRNFIVTPNPYAYFARVAQMFIDLATPQRAAGVHPSATIDPAAQVAA
CCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHH
SAVIGPHVTVEAGAVIEDGVQLDANVFVGRGTTIGAGSHLYPNASVYHGCKVGPRAIIHA
HHHCCCCEEEECCCEEECCEEECCEEEEECCCEECCCCCCCCCCCEECCCCCCCHHHEEE
GAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVTIGPDVEIGANTTIDRGAMADTVIE
CEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCEECCCCCCCCCHHHHHHHH
ECVKIDNQVQIGHNCRIGAYTVIAGSAGIAGSTTIGRHCMIGGAAGIAGHVTLGDYVIIT
HHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEE
AKSGVSKSLPKAGIYTSAFPAVDHGEWNKSAALVRNLDKLRERIKALEAALAAQGGTDA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALTLEELVKRFGGEIAGDAQCKVGGLAPLDQAGPQQLAFLANPKYLSQVESTRAGAVLI
CCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHEEEECCCHHHHHHHHCCCCEEEE
APKDLEKLGAAAGGRNFIVTPNPYAYFARVAQMFIDLATPQRAAGVHPSATIDPAAQVAA
CCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHH
SAVIGPHVTVEAGAVIEDGVQLDANVFVGRGTTIGAGSHLYPNASVYHGCKVGPRAIIHA
HHHCCCCEEEECCCEEECCEEECCEEEEECCCEECCCCCCCCCCCEECCCCCCCHHHEEE
GAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVTIGPDVEIGANTTIDRGAMADTVIE
CEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCEECCCCCCCCCHHHHHHHH
ECVKIDNQVQIGHNCRIGAYTVIAGSAGIAGSTTIGRHCMIGGAAGIAGHVTLGDYVIIT
HHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEE
AKSGVSKSLPKAGIYTSAFPAVDHGEWNKSAALVRNLDKLRERIKALEAALAAQGGTDA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA