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The map label for this gene is purR [H]

Identifier: 78066218

GI number: 78066218

Start: 1739732

End: 1740763

Strand: Direct

Name: purR [H]

Synonym: Bcep18194_A4748

Alternate gene names: 78066218

Gene position: 1739732-1740763 (Clockwise)

Preceding gene: 78066217

Following gene: 78066219

Centisome position: 47.09

GC content: 68.22

Gene sequence:

>1032_bases
ATGGCGACGATCAAGGATGTGGCGGCCATGGCGGGCGTGTCGTTTACGACCGTCTCGCACGTGGTGAACAATTCGCGGCC
GGTGTCCGCGGATGTGCGTGCGAAGGTCGAGGGCGCAATCCGCGAGCTGAATTACGTGCCGTCGGCCGTGGCGCGCTCGC
TGAAGGCGCGTGCGACGGCGACGATCGGCCTCGTCGTGCCGAACAGCACGAATCCGTACTTCGCGGAGCTTGCGCGCGGC
ATTGAGGATCAGTGCGCGGCCAATGGCTATTGCGTGTTCTTCTGCAACTCGGACGACGATCCCGTCAAGCAGCGCAACTA
CCTGCGCGTGCTGCAGGAAAAGCGTATCGACGGGCTGATCGTGGCATCGGCCGGCGAGGATGCGGTGCTCGCGCAGACGC
TCGCGGATATCCACGCACCGCTCGTCGTCGTCGACCGCAACATCGAGGGGCTCGCGGCCGACCTCGTGCAGATCGACCAC
GAGCGCGGTGCGTACCTGGCGACGCGCCATCTGCTCGAGCTCGGGCATGCGAAGATCGGCTGCATCACGGGGCCGACCGA
CACGGCGGTCAGCGCAATGCGCGTGCACGGCTTCATCCGTGCGATGGCCGAGCGCGGGGTCGACATCGTGCCGGGCGCGA
TCGCGGAAAGCGACTTCTCGTGCCTCGGCGGTTATCACGCGGCGTCGCGGCTGTTCGAATCGGTGCGGCCGAGCGCGATC
TTCGCGGGCAACGACTTGATGGGCGTCGGCGCGTTGCGCGCGGCGGCCGAGCGCGGCATGCGCGTGCCCGACGACTGTTC
GATCATCGGGTTCGACGACATTGAATTCTCCCGCTACACGTATCCGGCGCTGTCGACGGTCGGCCAATCGGTGCGCGCGC
TCGGCGAAATGGCCGCGCAGACGCTGATCGAGCGGATCGGCGGCGGCTCGACCGCCGTGCCGAGCCGCCGTCGCGTCGTG
TCGCCGCGCCTCGTGCTGCGCGAATCGACGGCGATCTACCGCGAGCCGCCGGCATCGGGCGGTCGCGCATGA

Upstream 100 bases:

>100_bases
GCGCGGTGATCGTGGTGGCGGTCGTGCTCGATACGTATCGCAGCCGGCGCACACGCGTGCGGTAGAAAGACAGACAGAAC
AGACCAGAGAGAAACGGGAA

Downstream 100 bases:

>100_bases
CGGCGCCCGCAGCACACACCGGCCGCGTGACGGTGGTCGGCAGCCTCAACATGGATCTGGTCGTGCGCGCGCCGCGTCTG
CCGCTGCCGGGCGAAACGCT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATATIGLVVPNSTNPYFAELARG
IEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLIVASAGEDAVLAQTLADIHAPLVVVDRNIEGLAADLVQIDH
ERGAYLATRHLLELGHAKIGCITGPTDTAVSAMRVHGFIRAMAERGVDIVPGAIAESDFSCLGGYHAASRLFESVRPSAI
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQTLIERIGGGSTAVPSRRRVV
SPRLVLRESTAIYREPPASGGRA

Sequences:

>Translated_343_residues
MATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATATIGLVVPNSTNPYFAELARG
IEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLIVASAGEDAVLAQTLADIHAPLVVVDRNIEGLAADLVQIDH
ERGAYLATRHLLELGHAKIGCITGPTDTAVSAMRVHGFIRAMAERGVDIVPGAIAESDFSCLGGYHAASRLFESVRPSAI
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQTLIERIGGGSTAVPSRRRVV
SPRLVLRESTAIYREPPASGGRA
>Mature_342_residues
ATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATATIGLVVPNSTNPYFAELARGI
EDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLIVASAGEDAVLAQTLADIHAPLVVVDRNIEGLAADLVQIDHE
RGAYLATRHLLELGHAKIGCITGPTDTAVSAMRVHGFIRAMAERGVDIVPGAIAESDFSCLGGYHAASRLFESVRPSAIF
AGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQTLIERIGGGSTAVPSRRRVVS
PRLVLRESTAIYREPPASGGRA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=40.4833836858006, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=42.2155688622754, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=33.4319526627219, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=36.7164179104478, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789202, Length=334, Percent_Identity=33.5329341317365, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=31.2312312312312, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786540, Length=305, Percent_Identity=31.4754098360656, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=29.6178343949045, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=25.3164556962025, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=26.6081871345029, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=27.1386430678466, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786268, Length=328, Percent_Identity=25.9146341463415, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=24.3670886075949, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790192, Length=248, Percent_Identity=27.8225806451613, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36452; Mature: 36321

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATA
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEE
TIGLVVPNSTNPYFAELARGIEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGEDAVLAQTLADIHAPLVVVDRNIEGLAADLVQIDHERGAYLATRHLLELGHAKIG
EECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEE
CITGPTDTAVSAMRVHGFIRAMAERGVDIVPGAIAESDFSCLGGYHAASRLFESVRPSAI
EECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQ
EECCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLIERIGGGSTAVPSRRRVVSPRLVLRESTAIYREPPASGGRA
HHHHHHCCCCCCCCCCHHHCCCHHEEECCCHHEECCCCCCCCC
>Mature Secondary Structure 
ATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATA
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEE
TIGLVVPNSTNPYFAELARGIEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGEDAVLAQTLADIHAPLVVVDRNIEGLAADLVQIDHERGAYLATRHLLELGHAKIG
EECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEE
CITGPTDTAVSAMRVHGFIRAMAERGVDIVPGAIAESDFSCLGGYHAASRLFESVRPSAI
EECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQ
EECCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLIERIGGGSTAVPSRRRVVSPRLVLRESTAIYREPPASGGRA
HHHHHHCCCCCCCCCCHHHCCCHHEEECCCHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA