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The map label for this gene is shiA [H]

Identifier: 78065861

GI number: 78065861

Start: 1348768

End: 1350078

Strand: Direct

Name: shiA [H]

Synonym: Bcep18194_A4390

Alternate gene names: 78065861

Gene position: 1348768-1350078 (Clockwise)

Preceding gene: 78065859

Following gene: 78065862

Centisome position: 36.51

GC content: 68.12

Gene sequence:

>1311_bases
ATGACCCAGTCCCTTCCTTCCGCCCGCCAGCCGGCGCGCGCCGCGACGGCCGCGTTCATCGGCACGATGATCGAGTGGTA
CGACTTCTACATCTACGCAACCGCCGCCGCGCTCGTGTTCGGCGAGCTCTATTTCCCGTCGCATGACCGCTTCGTCAGCA
CGATGGCGTCGTTCGCGACGTTCGCGGTCGGCTTCTTCGCGCGGCCGCTCGGCGGCGTGATCTTCGGCCATCTTGGGGAC
CGCATCGGCCGCAAGAAGGCGTTGATGACCACGCTGATGATGATGGGCGTCGCAACCGTCTGCGTCGGGCTGCTGCCCGA
TTATTCGAAGGTCGGCATGCTCGCGCCGGTGCTGCTCGTCGCGCTGCGCGTGGTGCAGGGGATCGCGGTCGGCGGCGAAT
GGGGCGGCGCGGTGCTGATGGCGGGCGAGCATGCGCCGCAGGGGCGCCGCACGTTCTTCGCGTCGTTCGCGCAGCTCGGC
AGCCCGGCCGGGCTGATCCTGTCGCTGATCGCGTTTCGCGCGGTCACGTCGATGGACAAGGCCGATTTCCTCGCATGGGG
CTGGCGCCTGCCGTTTCTCGCGAGTTCGGTGCTGCTCGTGGTCGGCATCCTGATCCGGCTCGGCGTGAACGAGTCGCCGG
AATTCGCGCGCGTGAAGGAAACCAACCGCACGGTGAAGCTGCCGGTCGCGGAAGTGTTCCGCTCCGCGTCGGGGCTCGTG
GCGCTCTGCATCGGCGCGAACACGATCGGCATCGCGGGCGTCTATTTCACGAACACGTTCATGATCGCGTACACGACGCA
GTACGTCGGCGTGTCGCGCTCGCTGATCCTCGACAGCCTGTTCGCGGTCGCGATCATCCAGTTCATCGCGCAGCCGATCG
CCGCGTGGATCGCCGAGCGCATCGGCGGCGCGCGTTTCCTGAAGCTCGCGGCGTTGCTCGCGATGCTGTCGCCGTACCCG
ATGTTCATGCTCGTGCAGGGCGGCACGTCCGCCTCGCTGATCGCAGGCATCGCGCTCGCGGTGGTCTGCATGGCCGGCTT
CTATTCGGTGATCGCGGGTTTCGTCAGCGACGTGTTCCCCGCGCACGTGCGCTATTCGGCGATCTCGCTCGCTTACCAGA
TCTGCGGCGCGATCGCGGGCGGGCTGACGCCGCTCGTCGGCACGTGGCTCGCCCACCGCTTCGCGGGCCAGTGGTGGCCG
CTCGCGGTGTTCTACACGTGTCTGGCCGGCATCTCGCTGCTTTGCATCGTCGCGCTCGACGCACGGCGCGCGGCACGGCC
GGCTGCTGCCGAAGCGCTCGGCTCGCAGTGA

Upstream 100 bases:

>100_bases
GATAAAGGAGGGGTGCCGCAGCGTGCGGCACAAGGCCGCGACAGACGGCCGTCACACGATATTGCGCGTCGCTCCGCTGC
GGCGCGCGTCAGGAGCCCGC

Downstream 100 bases:

>100_bases
CCCTCAACGAACGAATTCATCCGGAGTGCACATGAAAGACCTGCTTGAAATCGACGGTGCCCGTTTGTGGCAGAGCCTGG
CTGACATGGCGCGGGTCGGT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFATFAVGFFARPLGGVIFGHLGD
RIGRKKALMTTLMMMGVATVCVGLLPDYSKVGMLAPVLLVALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLG
SPAGLILSLIAFRAVTSMDKADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKETNRTVKLPVAEVFRSASGLV
ALCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFIAQPIAAWIAERIGGARFLKLAALLAMLSPYP
MFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFPAHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFAGQWWP
LAVFYTCLAGISLLCIVALDARRAARPAAAEALGSQ

Sequences:

>Translated_436_residues
MTQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFATFAVGFFARPLGGVIFGHLGD
RIGRKKALMTTLMMMGVATVCVGLLPDYSKVGMLAPVLLVALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLG
SPAGLILSLIAFRAVTSMDKADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKETNRTVKLPVAEVFRSASGLV
ALCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFIAQPIAAWIAERIGGARFLKLAALLAMLSPYP
MFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFPAHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFAGQWWP
LAVFYTCLAGISLLCIVALDARRAARPAAAEALGSQ
>Mature_435_residues
TQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFATFAVGFFARPLGGVIFGHLGDR
IGRKKALMTTLMMMGVATVCVGLLPDYSKVGMLAPVLLVALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGS
PAGLILSLIAFRAVTSMDKADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKETNRTVKLPVAEVFRSASGLVA
LCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFIAQPIAAWIAERIGGARFLKLAALLAMLSPYPM
FMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFPAHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFAGQWWPL
AVFYTCLAGISLLCIVALDARRAARPAAAEALGSQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=38.9791183294664, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790550, Length=438, Percent_Identity=31.2785388127854, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1789941, Length=418, Percent_Identity=32.5358851674641, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788942, Length=380, Percent_Identity=28.6842105263158, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=23.2067510548523, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082404, Length=400, Percent_Identity=22.5, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322632, Length=354, Percent_Identity=25.4237288135593, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46269; Mature: 46138

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFAT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FAVGFFARPLGGVIFGHLGDRIGRKKALMTTLMMMGVATVCVGLLPDYSKVGMLAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGSPAGLILSLIAFRAVTSMDK
HHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
ADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKETNRTVKLPVAEVFRSASGLV
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCHHHHHCCCCCEE
ALCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFIAQPIAAWIAER
EEEECCCCHHHHHHHHHHEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGARFLKLAALLAMLSPYPMFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFP
HCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFAGQWWPLAVFYTCLAGISLLCIVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARRAARPAAAEALGSQ
HHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFAT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FAVGFFARPLGGVIFGHLGDRIGRKKALMTTLMMMGVATVCVGLLPDYSKVGMLAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGSPAGLILSLIAFRAVTSMDK
HHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
ADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKETNRTVKLPVAEVFRSASGLV
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCHHHHHCCCCCEE
ALCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFIAQPIAAWIAER
EEEECCCCHHHHHHHHHHEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGARFLKLAALLAMLSPYPMFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFP
HCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFAGQWWPLAVFYTCLAGISLLCIVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARRAARPAAAEALGSQ
HHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]