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The map label for this gene is argT [H]

Identifier: 78065769

GI number: 78065769

Start: 1237137

End: 1237931

Strand: Direct

Name: argT [H]

Synonym: Bcep18194_A4297

Alternate gene names: 78065769

Gene position: 1237137-1237931 (Clockwise)

Preceding gene: 78065768

Following gene: 78065772

Centisome position: 33.49

GC content: 64.78

Gene sequence:

>795_bases
ATGAAGTTGGATTGGCGTAAAGTGGCCGCGCATGCGGTGGTGGCGGCATCGGCCGTGGCGGCAGGCAGCGCGATGGCAGC
GGATCTGAAAGAGATCCGTTTCGGCGTCGAAGCCTCGTACGCGCCGTTCGAATACAAGACGCCCGACGGCAAGCTGACCG
GCTTCGATATCGACATCGGCAACGCGGTGTGCGCGAAGCTGAAGACGAAGTGCGTGTGGGTCGAGAACGACTTCGACGGT
CTGATCCCGGCGCTGCAGGCACGCAAGTTCGACGCGATCAACTCGGACATGACGATCACCGACCAGCGCAAGCACGCGGT
CGCGTTCACCGACGCGATCTACACGATCCCGAACCAGCTGATCGCGAAGCAGGGCAGCGGCCTGCTGCCGACCCCGCAAT
CGCTGAAGGGCAAGCGCGTCGGCGTGCTGCAGGGCACGATCCAGGAAGCGTACGCGAAGAAGAAGTGGGCGCCGGCAGGC
GTCGAAGTCGTGCCGTACCAGACGCAGGACCTGGCCTACGCCGACCTGAAGTCGGGCCGTCTCGACGCGACGTTCCAGGA
CGCGGAAGCCGGTTCGAAGGGCTTCCTGACGAAGCCGCAAGGCCAGGGCTTCGCGTTCGCGGGCGGTACCGTGAGCGACG
CCGAGATCCTCGGCTCGGGCGTCGGTTTCGGCCTGCGCAAGAACGACGCGGCGCTGAAGACCGCGCTCGATCAGGCGCTG
AAGGAACTGAAGGCCGACGGCACGATCGACAACCTCGCGAAGAAGTACTTCAGCGTGCCCGTGACGCTCAAGTAA

Upstream 100 bases:

>100_bases
CGCATCACGGCGCGGCGGCGGCATTGCCGTGGTCATCCGACCCTGCAATGATGCATGCGCCCGTAGTTCCGGAACATTCG
AGTATCGAGGAGAACACCTG

Downstream 100 bases:

>100_bases
GCGTTGCGTTCGATCGCACGATGCAAGCAAACCGGCCGCTGATGCGGCCGGTTTGTTTTTGGGGGCGGGAAAGGACGGGA
AGCGGTGCGGCCGCGCCCGG

Product: ABC transporter, periplasmic ligand binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKLDWRKVAAHAVVAASAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKTKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAVAFTDAIYTIPNQLIAKQGSGLLPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDAEAGSKGFLTKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
KELKADGTIDNLAKKYFSVPVTLK

Sequences:

>Translated_264_residues
MKLDWRKVAAHAVVAASAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKTKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAVAFTDAIYTIPNQLIAKQGSGLLPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDAEAGSKGFLTKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
KELKADGTIDNLAKKYFSVPVTLK
>Mature_264_residues
MKLDWRKVAAHAVVAASAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKTKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAVAFTDAIYTIPNQLIAKQGSGLLPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDAEAGSKGFLTKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
KELKADGTIDNLAKKYFSVPVTLK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=241, Percent_Identity=52.2821576763486, Blast_Score=263, Evalue=9e-72,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=49.5689655172414, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=35.1020408163265, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=32.6530612244898, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=32.0610687022901, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=32.2709163346614, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28103; Mature: 28103

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDWRKVAAHAVVAASAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIG
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKTKCVWVENDFDGLIPALQARKFDAINSDMTITDQRKHAVAFTDAIYTIPNQL
HHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHHH
IAKQGSGLLPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAGVEVVPYQTQDLAYADLKSGR
HHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCC
LDATFQDAEAGSKGFLTKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
CCCEECCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KELKADGTIDNLAKKYFSVPVTLK
HHHHCCCCHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MKLDWRKVAAHAVVAASAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIG
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKTKCVWVENDFDGLIPALQARKFDAINSDMTITDQRKHAVAFTDAIYTIPNQL
HHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHHH
IAKQGSGLLPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAGVEVVPYQTQDLAYADLKSGR
HHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCC
LDATFQDAEAGSKGFLTKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
CCCEECCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KELKADGTIDNLAKKYFSVPVTLK
HHHHCCCCHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]