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The map label for this gene is rlmI [H]

Identifier: 78050032

GI number: 78050032

Start: 5163005

End: 5164171

Strand: Reverse

Name: rlmI [H]

Synonym: XCV4476

Alternate gene names: 78050032

Gene position: 5164171-5163005 (Counterclockwise)

Preceding gene: 78050035

Following gene: 78050031

Centisome position: 99.72

GC content: 64.27

Gene sequence:

>1167_bases
ATGAATATTCCAGTCCCCGTCGTCCGTCTCAAGAATGCCTGGCGCTCCAGCCACCCGTGGATCTTCCAGAAGCTGGTGGA
AAAGCCCGCCGCCAAGCCCAAGCCCGGCACCATCGTCGATGTGGTCGGCGTGGATGGGGAATGGATTGGCCGCGGCTTCT
ACAACGGCCATTCGCGCATTGCCGTGCGCATCCTGGAAACCGACCAGTCGGTGCCGGTGGATGCGGGCTGGTTCTCGCGC
AAGATCGCCGCGGCAGTGAGCCTGCGCCGCGACTGGCTCAAGCTCGATGGCGTGTCCGACGCCTGGCGCGTGGCGCACAG
CGAAGGCGATGGCCTGTCCGGCCTGGTGGTGGACCGCTACGGCGACCTGGTGGTGGTGGAGTTCTTCGCCGCCGGCATGT
TCCGTCATCGCGAATGGATCTACGAGGCGCTGCGCGAACAGTTCCCCGGCTGCCGCTTCCACAGCTTTGCCGACGAACAT
GTGCAAAAGCAGGAAAGCTTCGATTTCCACAGCAACACCACCACCGAAGCGGCGGTGATCACCGAGCACGGCATCAAGTT
CCGCGCCGACCCGGCCGGTGCGCACAAGACCGGTTTTTTCGCCGACCAGCGCGACAACCGCCAGTGGCTGAGCGAGCAGG
TCGAAGGCAAGAGCGTGCTGGACCTGTGCTGCAACACCGGCGGCTTTGCGGTGTATGCCGCCGCGCGCGGTGCGGCCGAA
GTGCTGGGCGTGGACATCGACGAAGACGTGATTGCCATTGCCAAGGGCAACGCCAAGCTCAATAACGTGCGGCCCAAGTT
CGTGCAGGCCGACATCTTTCCGTGGCTGCGCGATGCGGCCAACCGCGGCGAGCAGTTCGATGTGGTGATCCTGGACCCGG
CCAAGATGACCCGCGACCGCGATCAGGTGATTCCGGCGCTGAAGAAGTACCTGGACATGAACAAGCTGGCCTTGGGCGTG
GTCAAGCCGGGCGGCCTGTTTGCCACCTTCTCGTGCACCGGCCTGGTGGCCGAGCAGGAGTTCCTGGACATGCTGCGGCG
CGCGTCGTATTTCTCCGGGCGCACCATCCAGATCCTGAAGGTGGCCGGTGCCGGTCCCGATCATCCGTTCATGGCGCACG
TGCAGGAATCGCGCTATCTGAAAGCGGTGTTCTGCCGGGTGATGTAG

Upstream 100 bases:

>100_bases
GCTCGTAATGACGGCGACAACGCTGGGAGCTACTCCCCTTCTGCGCCCGATTCTGCGTGCACGGCGCAGGCACGTCAATC
CAGCCGGATACAATGGACCG

Downstream 100 bases:

>100_bases
CAGCGCGTTGCGGCGAAAGACGCCCGCTGCCGTGTGGCAGGCAGCGTCTTTCGCGGCGCGATGCCATCGCCGGCCAGCAG
TAGGTCAACGCAGACATGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRIAVRILETDQSVPVDAGWFSR
KIAAAVSLRRDWLKLDGVSDAWRVAHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDRDQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVM

Sequences:

>Translated_388_residues
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRIAVRILETDQSVPVDAGWFSR
KIAAAVSLRRDWLKLDGVSDAWRVAHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDRDQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVM
>Mature_388_residues
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRIAVRILETDQSVPVDAGWFSR
KIAAAVSLRRDWLKLDGVSDAWRVAHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDRDQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=379, Percent_Identity=35.3562005277045, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1787181, Length=197, Percent_Identity=27.4111675126904, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43277; Mature: 43277

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRI
CCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEECCCEECCCCEE
AVRILETDQSVPVDAGWFSRKIAAAVSLRRDWLKLDGVSDAWRVAHSEGDGLSGLVVDRY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHCCCCCCCCEEEEECC
GDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEHVQKQESFDFHSNTTTEAAVI
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
TEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
EECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHH
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDR
HEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCH
DQVIPALKKYLDMNKLALGVVKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILK
HHHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKAVFCRVM
EECCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRI
CCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEECCCEECCCCEE
AVRILETDQSVPVDAGWFSRKIAAAVSLRRDWLKLDGVSDAWRVAHSEGDGLSGLVVDRY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHCCCCCCCCEEEEECC
GDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEHVQKQESFDFHSNTTTEAAVI
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
TEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
EECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHH
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDR
HEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCH
DQVIPALKKYLDMNKLALGVVKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILK
HHHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKAVFCRVM
EECCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA