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The map label for this gene is ruvC [H]

Identifier: 78048837

GI number: 78048837

Start: 3748041

End: 3748565

Strand: Reverse

Name: ruvC [H]

Synonym: XCV3281

Alternate gene names: 78048837

Gene position: 3748565-3748041 (Counterclockwise)

Preceding gene: 78048838

Following gene: 78048836

Centisome position: 72.39

GC content: 67.05

Gene sequence:

>525_bases
ATGACCCGCATCCTGGGCATCGATCCCGGTTCGCAGCGCACCGGCATCGGCATCATCGATATCGACGAGGACGGTCGCAG
CCGGCATGTGCACCACGCGCCGCTGATGCTGCTGGGGGAGGGTGATTTCTCCCAACGGCTCAAGCGGCTGCTGCAGGGGC
TGGGCGAGCTGATCGAGACCTACCGGCCGGATGAGGTGGCGATCGAAAGGGTGTTCATGGGCAAGAGCGCCGCTTCGGCG
CTCAAGCTCGGCCACGCGCGCGGCGCGGCGATCTGCGCGGTGGTCATGCGCGACCTGCCGGTGCACGAGTACGCTGCCAC
CGAAGTCAAGCTCGCGCTGGTCGGCAAGGGCGGCGCGGACAAGGTGCAGGTCCAGCACATGGTCGGCATCATGCTCAACC
TGAAAGGCAAGCTGCAGCCCGACGCCGCCGACGCATTGGCGGTCGCCATCACTCACGCCCACGTGCGCGCCACCGCGCAA
CGCCTGGGCGTCAATACCCAACAGGCCTGGAGCCGCAAGAAATGA

Upstream 100 bases:

>100_bases
AATCGAAACAGCAGCATCCTGCATCGCTCGGCAATGTCGAGCACGCCAGTAGGCCGCTTTACCCATTCCCGATTCCCGAC
TCCCAATTGCCGGCCGCTCA

Downstream 100 bases:

>100_bases
CAATCGGGATTCGGGATTTGGGATTGGGGATTCGCAACCGCGAGTTCGCGTGCGCTGTACCGCTTCGGCGGTGCGCAGCC
GTCGTCAACGGCAGCGAGGG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTRILGIDPGSQRTGIGIIDIDEDGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIETYRPDEVAIERVFMGKSAASA
LKLGHARGAAICAVVMRDLPVHEYAATEVKLALVGKGGADKVQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQ
RLGVNTQQAWSRKK

Sequences:

>Translated_174_residues
MTRILGIDPGSQRTGIGIIDIDEDGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIETYRPDEVAIERVFMGKSAASA
LKLGHARGAAICAVVMRDLPVHEYAATEVKLALVGKGGADKVQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQ
RLGVNTQQAWSRKK
>Mature_173_residues
TRILGIDPGSQRTGIGIIDIDEDGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIETYRPDEVAIERVFMGKSAASAL
KLGHARGAAICAVVMRDLPVHEYAATEVKLALVGKGGADKVQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQR
LGVNTQQAWSRKK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=169, Percent_Identity=50.887573964497, Blast_Score=126, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18779; Mature: 18648

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRILGIDPGSQRTGIGIIDIDEDGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIET
CCEEEECCCCCCCCCEEEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
YRPDEVAIERVFMGKSAASALKLGHARGAAICAVVMRDLPVHEYAATEVKLALVGKGGAD
CCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCEEEEEEEECCCCC
KVQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQRLGVNTQQAWSRKK
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
TRILGIDPGSQRTGIGIIDIDEDGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIET
CEEEECCCCCCCCCEEEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
YRPDEVAIERVFMGKSAASALKLGHARGAAICAVVMRDLPVHEYAATEVKLALVGKGGAD
CCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCEEEEEEEECCCCC
KVQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQRLGVNTQQAWSRKK
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]