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The map label for this gene is ycgF [H]

Identifier: 78046766

GI number: 78046766

Start: 1355558

End: 1356304

Strand: Reverse

Name: ycgF [H]

Synonym: XCV1210

Alternate gene names: 78046766

Gene position: 1356304-1355558 (Counterclockwise)

Preceding gene: 78046770

Following gene: 78046762

Centisome position: 26.19

GC content: 64.79

Gene sequence:

>747_bases
ATGACCACGCTTCCTGTCTGCAAAGCCTGCCGCGACGGCCAGGACTTCCCCACCGCGATCACCATGGCCTTCCAGCCCAT
CGTGGACGTGTCCACCCGCAGCATCTACGCAGGCGAGGCGCTGGTGCGCGGCCTCAACGGCGAGTCGGCCGGCAGCATCC
TGGCCGCAGTGGATGAGCGCAATCGCTACGCATTCGACCAGGCCTGCCGCATCAAGGCAATCGAATGCGCTGCGCAGATT
GGCTTGCCGGCGCTGCTGTCGATCAACTTCATGCCCAACGCGGTCTATAACCCCGAGCATTGCCTGCGCGCCACGCTGTC
GGCCACTGCGCAATACGGTTGGCCGCTCGACGCCATCATCTTCGAGGTCAGCGAACAGGAACATCTCACCGATCCGGCGC
ATCTGCTCGGCATCCTGCGCACCTATCAGGCCCGCGGCATGAAAACGGCCATCGACGACTTCGGCGCCGGCTACGCCGGG
CTCGGCCTGCTGGCCGAGTTCCAGCCGGATCTGCTCAAGTTGGACATCGCCCTGGTCCGCGGGATCGACACCGACCGCAG
CCGCCAAGCCATCGTGCGCCACGTGACGCGCATGTGCGAAGAACTCGGCATCGCCGTCATTGCAGAGGGCATCGAACAAC
CCGATCAATACCGCGCCCTGCGCGACCTGGGCATCCACCTGCAGCAAGGCTATCTGTTCGCCAGACCGCAGATCGGCGAG
CTGCCGAAGGTGCATTGGCCGCAGTAA

Upstream 100 bases:

>100_bases
CGACAGGTGAGGATGCTGCCATGAGCGATTCAGGCTCAATCCCACGCGATTGCGGCCGATAGGTCTAGGCTGCACCTACG
CTGTTCGATGACCTGACGCC

Downstream 100 bases:

>100_bases
GCTCACGCTTGTGCGTATGAACTAAACAACTCAGCGCACCTAGCAGCAGGCGCCACTCGCCGTAACAGACCCACAACATC
CGAACACAGCGGCAATGAAA

Product: putative phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTTLPVCKACRDGQDFPTAITMAFQPIVDVSTRSIYAGEALVRGLNGESAGSILAAVDERNRYAFDQACRIKAIECAAQI
GLPALLSINFMPNAVYNPEHCLRATLSATAQYGWPLDAIIFEVSEQEHLTDPAHLLGILRTYQARGMKTAIDDFGAGYAG
LGLLAEFQPDLLKLDIALVRGIDTDRSRQAIVRHVTRMCEELGIAVIAEGIEQPDQYRALRDLGIHLQQGYLFARPQIGE
LPKVHWPQ

Sequences:

>Translated_248_residues
MTTLPVCKACRDGQDFPTAITMAFQPIVDVSTRSIYAGEALVRGLNGESAGSILAAVDERNRYAFDQACRIKAIECAAQI
GLPALLSINFMPNAVYNPEHCLRATLSATAQYGWPLDAIIFEVSEQEHLTDPAHLLGILRTYQARGMKTAIDDFGAGYAG
LGLLAEFQPDLLKLDIALVRGIDTDRSRQAIVRHVTRMCEELGIAVIAEGIEQPDQYRALRDLGIHLQQGYLFARPQIGE
LPKVHWPQ
>Mature_247_residues
TTLPVCKACRDGQDFPTAITMAFQPIVDVSTRSIYAGEALVRGLNGESAGSILAAVDERNRYAFDQACRIKAIECAAQIG
LPALLSINFMPNAVYNPEHCLRATLSATAQYGWPLDAIIFEVSEQEHLTDPAHLLGILRTYQARGMKTAIDDFGAGYAGL
GLLAEFQPDLLKLDIALVRGIDTDRSRQAIVRHVTRMCEELGIAVIAEGIEQPDQYRALRDLGIHLQQGYLFARPQIGEL
PKVHWPQ

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=231, Percent_Identity=32.9004329004329, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790496, Length=232, Percent_Identity=29.3103448275862, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI226510982, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081980, Length=151, Percent_Identity=31.7880794701987, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87081921, Length=232, Percent_Identity=28.448275862069, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87082096, Length=152, Percent_Identity=30.9210526315789, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788502, Length=245, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081845, Length=154, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788849, Length=152, Percent_Identity=32.2368421052632, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=122, Percent_Identity=34.4262295081967, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787055, Length=226, Percent_Identity=28.7610619469027, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081743, Length=125, Percent_Identity=32.8, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786507, Length=128, Percent_Identity=27.34375, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 27262; Mature: 27130

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLPVCKACRDGQDFPTAITMAFQPIVDVSTRSIYAGEALVRGLNGESAGSILAAVDER
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEHHCC
NRYAFDQACRIKAIECAAQIGLPALLSINFMPNAVYNPEHCLRATLSATAQYGWPLDAII
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
FEVSEQEHLTDPAHLLGILRTYQARGMKTAIDDFGAGYAGLGLLAEFQPDLLKLDIALVR
EECCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
GIDTDRSRQAIVRHVTRMCEELGIAVIAEGIEQPDQYRALRDLGIHLQQGYLFARPQIGE
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCC
LPKVHWPQ
CCCCCCCC
>Mature Secondary Structure 
TTLPVCKACRDGQDFPTAITMAFQPIVDVSTRSIYAGEALVRGLNGESAGSILAAVDER
CCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEHHCC
NRYAFDQACRIKAIECAAQIGLPALLSINFMPNAVYNPEHCLRATLSATAQYGWPLDAII
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
FEVSEQEHLTDPAHLLGILRTYQARGMKTAIDDFGAGYAGLGLLAEFQPDLLKLDIALVR
EECCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
GIDTDRSRQAIVRHVTRMCEELGIAVIAEGIEQPDQYRALRDLGIHLQQGYLFARPQIGE
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCC
LPKVHWPQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]