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The map label for this gene is glcD [H]

Identifier: 78046370

GI number: 78046370

Start: 928831

End: 930264

Strand: Reverse

Name: glcD [H]

Synonym: XCV0814

Alternate gene names: 78046370

Gene position: 930264-928831 (Counterclockwise)

Preceding gene: 78046371

Following gene: 78046369

Centisome position: 17.96

GC content: 68.76

Gene sequence:

>1434_bases
ATGCATCCAGCGCGCGGCACCACAGGATCGCATGTCATGACCGATGTACTTCCCACCGCACTTGCCGAGGTGTTGGCCAC
GCGCCTGGGTACCGATGGCTGGTTGACCGGCGACGATGCGCGCCGCCGCTATGGCGAAGACGATTCGCGCCGCTGGGCAT
TGCCTGCCGCAGTGGCGTTGCCGCGCGACACCGATGAGGTGGTGGCGATCGTGCAGGCGTGCCGTGCGCACGGCGTGCCG
ATCGTGGCGCGCGGCGCCGGCACCGGCACCACCGGCGCGGCGGTGCCGTTTTCCGGCGGCGTGGTGGTGTCGATGGCGCG
CATGAACCAGATCCTGGCACTGCGTCCGCAAGACCGCTGCGCGGTGGTGCAACCGGGCGTGCTCAATGGCGACCTGCAGC
AGGCGCTGCAGCCGCACGGATTGTTCTGGCCGCCGGATCCGTCCAGCGCGGAGATCTGCAGCGTCGGCGGCAATCTATCC
ACCAACGCCGGCGGCCCGCGCGCGGTGAAATACGGCGCCACCCGCGACAACGTGCTCGGCCTGGTGGCCGTCACCGGCAC
CGGCGAGGTGATCCGCTGCGGCGGTGCGTACACCAAGAATTCCACCGGTTACGACCTCACCCACTTGCTGGTGGGCAGCG
AGGGCACCCTGGCGATCATCGTCGAGGCGACCTTGAAGCTGACCCCGCGCGCAATCGCGCAAGCCGGCCTGCGCGCGCTG
TATCGCGATGCCTCCAGCGCCGCAGCCGCAGTGTCGCGGCTGATGGCGCAACCGACCACGCCGACCATGCTGGAATTCAT
GGATGCCAGCGCGATTGCGCTGCTACGCCGCAATGGCAGCGACGTACCCGACGCCGGCGCCATGCTGTTGATCGAAGCCG
ACGGCGATCACGACACCTTGCCCTACGCGCTGCAAGCCCTGCACGAGGCGGCCGATGGCCAGGGCGTACTGGGTCTGGAC
GTGGCCGCCGATGGCAGCGCGCGCGACAGGTTGTGGGCCGCACGCCGTGCCTTGTCGCCGGCCTTGCGCACCATCAAGCC
GGGCAAGATCAATGAGGACGTGGTGGTGCCGGTGTCGCGCATTCCCGAACTCGTCGCCGGCGTGGAAGCACTCGCTGCGG
AATTCGCGCTGCCCATCGTCGCCTTCGGCCATGCCGGCAACGGCAACCTGCACGTCAACATCATGTACGACCCGGCCGAT
GCCGACGAAGATGCCCGCGCACACGCCGCCCTGCCACGGCTGTTTACGCTGGTGCTGGCGCTGGAGGGCACGCTCTCCGG
CGAACACGGCATCGGCGTGGCCAAGCGCGATTTCATGAGCCAGGCGTTCAATCCAGCCACGCTGGCGGCCATGCGCGCGA
TCAAGGCGGCGCTGGACCCGGATGGCATCTTGAATCCGGGGAAGGTGTTGCCCCACTCGCCTGCCGGGCGATAG

Upstream 100 bases:

>100_bases
CATTTGCCACCGGCTTCTTCAATGCGCGTGGCCTGAGCACGCTGCTCTCCGAGCTGCGCTGGGTCTGGCTGCCGCTCACC
GTCGCCGTGCTGGGGTGGAA

Downstream 100 bases:

>100_bases
TCCGATGCAGACCCACACCATGAGTCAGTTGCTTCAGAAAGCACTCGATCCATACCGCAACCAGGCGATGGAACGGCTGC
TCATCGCACGCGATGCGTTT

Product: putative glycolate oxidase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MHPARGTTGSHVMTDVLPTALAEVLATRLGTDGWLTGDDARRRYGEDDSRRWALPAAVALPRDTDEVVAIVQACRAHGVP
IVARGAGTGTTGAAVPFSGGVVVSMARMNQILALRPQDRCAVVQPGVLNGDLQQALQPHGLFWPPDPSSAEICSVGGNLS
TNAGGPRAVKYGATRDNVLGLVAVTGTGEVIRCGGAYTKNSTGYDLTHLLVGSEGTLAIIVEATLKLTPRAIAQAGLRAL
YRDASSAAAAVSRLMAQPTTPTMLEFMDASAIALLRRNGSDVPDAGAMLLIEADGDHDTLPYALQALHEAADGQGVLGLD
VAADGSARDRLWAARRALSPALRTIKPGKINEDVVVPVSRIPELVAGVEALAAEFALPIVAFGHAGNGNLHVNIMYDPAD
ADEDARAHAALPRLFTLVLALEGTLSGEHGIGVAKRDFMSQAFNPATLAAMRAIKAALDPDGILNPGKVLPHSPAGR

Sequences:

>Translated_477_residues
MHPARGTTGSHVMTDVLPTALAEVLATRLGTDGWLTGDDARRRYGEDDSRRWALPAAVALPRDTDEVVAIVQACRAHGVP
IVARGAGTGTTGAAVPFSGGVVVSMARMNQILALRPQDRCAVVQPGVLNGDLQQALQPHGLFWPPDPSSAEICSVGGNLS
TNAGGPRAVKYGATRDNVLGLVAVTGTGEVIRCGGAYTKNSTGYDLTHLLVGSEGTLAIIVEATLKLTPRAIAQAGLRAL
YRDASSAAAAVSRLMAQPTTPTMLEFMDASAIALLRRNGSDVPDAGAMLLIEADGDHDTLPYALQALHEAADGQGVLGLD
VAADGSARDRLWAARRALSPALRTIKPGKINEDVVVPVSRIPELVAGVEALAAEFALPIVAFGHAGNGNLHVNIMYDPAD
ADEDARAHAALPRLFTLVLALEGTLSGEHGIGVAKRDFMSQAFNPATLAAMRAIKAALDPDGILNPGKVLPHSPAGR
>Mature_477_residues
MHPARGTTGSHVMTDVLPTALAEVLATRLGTDGWLTGDDARRRYGEDDSRRWALPAAVALPRDTDEVVAIVQACRAHGVP
IVARGAGTGTTGAAVPFSGGVVVSMARMNQILALRPQDRCAVVQPGVLNGDLQQALQPHGLFWPPDPSSAEICSVGGNLS
TNAGGPRAVKYGATRDNVLGLVAVTGTGEVIRCGGAYTKNSTGYDLTHLLVGSEGTLAIIVEATLKLTPRAIAQAGLRAL
YRDASSAAAAVSRLMAQPTTPTMLEFMDASAIALLRRNGSDVPDAGAMLLIEADGDHDTLPYALQALHEAADGQGVLGLD
VAADGSARDRLWAARRALSPALRTIKPGKINEDVVVPVSRIPELVAGVEALAAEFALPIVAFGHAGNGNLHVNIMYDPAD
ADEDARAHAALPRLFTLVLALEGTLSGEHGIGVAKRDFMSQAFNPATLAAMRAIKAALDPDGILNPGKVLPHSPAGR

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=477, Percent_Identity=33.5429769392034, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI37595754, Length=500, Percent_Identity=32.2, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI119964728, Length=426, Percent_Identity=32.8638497652582, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI4501993, Length=226, Percent_Identity=29.646017699115, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789351, Length=423, Percent_Identity=37.5886524822695, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1787977, Length=520, Percent_Identity=29.0384615384615, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI48994907, Length=482, Percent_Identity=23.8589211618257, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994914, Length=140, Percent_Identity=35, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=456, Percent_Identity=31.359649122807, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI71992381, Length=456, Percent_Identity=30.7017543859649, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI71992392, Length=380, Percent_Identity=31.5789473684211, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17534361, Length=469, Percent_Identity=26.865671641791, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17556096, Length=225, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320023, Length=452, Percent_Identity=28.3185840707965, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320027, Length=440, Percent_Identity=28.6363636363636, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6320764, Length=438, Percent_Identity=29.2237442922374, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI18921117, Length=425, Percent_Identity=30.3529411764706, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24639277, Length=425, Percent_Identity=30.3529411764706, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24639275, Length=425, Percent_Identity=30.3529411764706, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24653753, Length=242, Percent_Identity=29.7520661157025, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 49464; Mature: 49464

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPARGTTGSHVMTDVLPTALAEVLATRLGTDGWLTGDDARRRYGEDDSRRWALPAAVAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEECCHHEEC
PRDTDEVVAIVQACRAHGVPIVARGAGTGTTGAAVPFSGGVVVSMARMNQILALRPQDRC
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEHHHHCCEEEECCCCCE
AVVQPGVLNGDLQQALQPHGLFWPPDPSSAEICSVGGNLSTNAGGPRAVKYGATRDNVLG
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCEEE
LVAVTGTGEVIRCGGAYTKNSTGYDLTHLLVGSEGTLAIIVEATLKLTPRAIAQAGLRAL
EEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHHHHH
YRDASSAAAAVSRLMAQPTTPTMLEFMDASAIALLRRNGSDVPDAGAMLLIEADGDHDTL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHH
PYALQALHEAADGQGVLGLDVAADGSARDRLWAARRALSPALRTIKPGKINEDVVVPVSR
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHH
IPELVAGVEALAAEFALPIVAFGHAGNGNLHVNIMYDPADADEDARAHAALPRLFTLVLA
HHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LEGTLSGEHGIGVAKRDFMSQAFNPATLAAMRAIKAALDPDGILNPGKVLPHSPAGR
HHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHPARGTTGSHVMTDVLPTALAEVLATRLGTDGWLTGDDARRRYGEDDSRRWALPAAVAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEECCHHEEC
PRDTDEVVAIVQACRAHGVPIVARGAGTGTTGAAVPFSGGVVVSMARMNQILALRPQDRC
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEHHHHCCEEEECCCCCE
AVVQPGVLNGDLQQALQPHGLFWPPDPSSAEICSVGGNLSTNAGGPRAVKYGATRDNVLG
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCEEE
LVAVTGTGEVIRCGGAYTKNSTGYDLTHLLVGSEGTLAIIVEATLKLTPRAIAQAGLRAL
EEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHHHHH
YRDASSAAAAVSRLMAQPTTPTMLEFMDASAIALLRRNGSDVPDAGAMLLIEADGDHDTL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHH
PYALQALHEAADGQGVLGLDVAADGSARDRLWAARRALSPALRTIKPGKINEDVVVPVSR
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHH
IPELVAGVEALAAEFALPIVAFGHAGNGNLHVNIMYDPADADEDARAHAALPRLFTLVLA
HHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LEGTLSGEHGIGVAKRDFMSQAFNPATLAAMRAIKAALDPDGILNPGKVLPHSPAGR
HHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]