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The map label for this gene is gacS [H]

Identifier: 78046084

GI number: 78046084

Start: 593039

End: 595348

Strand: Reverse

Name: gacS [H]

Synonym: XCV0528

Alternate gene names: 78046084

Gene position: 595348-593039 (Counterclockwise)

Preceding gene: 78046085

Following gene: 78046078

Centisome position: 11.5

GC content: 68.92

Gene sequence:

>2310_bases
GTGAGAGAGCGGGCAGCAGGTCTTCGCGCCTGGAGCATCTTGGCAGTGCTGGCCGCGCTGGCGGCCGCACTCGGCGCAGG
CCTGCAATGGTCGTCGCTGCAAGGCCCCTGGCGCGCCGTCAGCGCGGTGTCGGCAGTACTGGCGGTGGTCTGCGGGCTGT
GCGCCGCACTTGCGGCCCACCGGGCTGCCCAGGCGCAGCATGCGTTGGCACAGCGCCTGGAAGAGGCCGACCTGGCCCGC
GAACGCTTGCAGCAGGAACTGCGGCGCCACGGCGAACTCGAACAGGAACTGTTGCGCGCCAAGCAGGCCGCCGAGGCCGC
CACCCTGGCCAAGGGCGAATTCCTGGCCACCATGAGTCACGAGATCCGCACCCCGCTCAACGGCATCATTCCGATGCTGG
AGCTGATCTCCAGTGGCCAGCTCAGCGTTGACCAGCGCGACATGCTGCAGACCGCCACCGGCAGCTCGCTGCAGCTGCTG
CGCATCGTCGACGACATCCTCGATTACTCCAAGCTCGAGGCCAACAAGCTCGAACTGGAAATCACCACCTTCAACCTACG
CGAGCTGCTCGACGGCGTCATCCAGTTGCTGCAGCGCACCGCCGAAGGCCGCCAGCTGCGCCTGAGCCTGGATATCGAAC
CGTCGGTACGGCTGCTGGTGCGCGGCGACCCGATCCGCCTGCGCCAGGTGCTGGGCAACCTGATCGGCAACGCCATCAAG
TTCACCGAACGCGGCAGCGTCGACATCCAGCTGCGCCGCCTGGGCGAAACGCGCGCCCAGCACCTGCTGCGCTTCCAGGT
GCGCGACACCGGCATCGGCATCGCCCCGGACCAGCAGGCACGCCTGTTCCGCTCCTTCGCACAAGCCGATGCCTCCACCA
CGCGGCTGTACGGCGGCACCGGCCTGGGGCTGGCGATCTGCAAGCGCATCATCGACCTGATGGGCGGCCGCATCGGGGTG
GAATCCGAACCCGGGCGCGGCTCCACCTTCTGGTTCGAAATCCCGCTGCTCAAGGTCATCGGCGACCTCCAGCAGGCCAC
CGGCGCCGATGCCGCACGGGTGATGGTGATCAGCTCCGATGCGCGCCTGTCGCAACGCCTCAAGCGCCTGCTCGACAGCT
GGGGGGTGTCGCACGTGCTCATGGAAACCACCCAGGAAGCGCTCGAACGCGTGCGCCGGCACAACGACAGCGAGGGCTTC
CGCTGCGTCATCGCCGACCACGACACGTTGCGCTACAGCGCGCGCGCGGTGCACCGCGCGCTCGCCCGCCCGGAAAACGC
CAACAGCGCGCGGCTGATCTGGCTGTACGGCGAAGAACCGGTGCCGGCCGAACTGCAGGATCACGCCACCCTGGTGCCGC
GCCAGAGCCCGGACGAAACCCTGCGCGCCCTGGTGCTGCCGCCCGAACCCAAGCCCGCCCACGTCACCACGCTGGCCTCG
GCGATGATCCCCGAGCCATTGCCGCCGCCGATGGCCAATGCGCGCGAGGTGCGCATCCTGCTGGTCGAAGACAACCCGGT
GAACCTGCTGGTCGCGCAGAAGCTGCTGGCCGTGCTTGGCTTCGAGGCCGACACCGCCACCGACGGTGAAGCCGCACTGG
CCAGCATGGAATCCACCCGCTACGACATGGTCTTCATGGATTGCCAGATGCCGGTGCTGGATGGCTATGCCGCCACCCGC
CGCTGGCGCGCGATGGAAACCGAGAGCGGCGGCCGCCCGATTCCGATCGTGGCCATGACCGCCAACGCCATGGCCGGCGA
TCGCGAACGCTGCCTGGCCGCCGGCATGGACGACTATCTGTCCAAGCCGGTCGCACGCGAACAGCTCGACGCCTGTCTGC
AGCGCTGGCTGCCGCGTCAGGCGCTGCTGCCCGGCCCAAGCAGCGCCGGCCAGGCGACCCACGACCCCGAAAGCGCCAGC
GCCGCTGCCCAGGCACGCGCACTGCCGATCCTGGACAACAACGTCATCGACGAGCTGTACGAAGTGGCCGGCGCGGACAC
CATCACCATCCTGCAGCTGTTCCTGGAAGACGCACCGCTGATCATCGAGCAACTGGAAGCGGCCGCCGCCAACCGCGACA
GCATGCAGCTGCGCGACCTCGCCCACACCCTCAAGTCCTCCAGCGCCAACGTCGGTGCGCAGGCGGTTTCCAACGCGGCC
CGGCGCATCGAACTGGCCGCCCGCACCGGCACCATCGAACGCCCCTCGGTCATGGTCGCCCTGGTCATCGCCGAATACGC
CCGCGCCCGCCTGGCGCTGCTAGGCCAGGTCGCCCGCCTGCAAGCCGCCGCCAGCACCACAAATCAGTAG

Upstream 100 bases:

>100_bases
GGCTGCGATGTGGATGAGCGGCGTGGATTTCATCCAGGGCAACCTGGTGCAGACGGTCGGCAAGGAACTGGACTTCGACT
TCACGAGCGCGGTGCTCTGA

Downstream 100 bases:

>100_bases
TCGCATCAGCGCTGCCGAAGCAGCAGCAGACGCCTGGCGCCGCGACCTGCATCGAACTGCTGAGGGATGCCATCTCGGCC
GCGAACACGCTCGGGCACAG

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MRERAAGLRAWSILAVLAALAAALGAGLQWSSLQGPWRAVSAVSAVLAVVCGLCAALAAHRAAQAQHALAQRLEEADLAR
ERLQQELRRHGELEQELLRAKQAAEAATLAKGEFLATMSHEIRTPLNGIIPMLELISSGQLSVDQRDMLQTATGSSLQLL
RIVDDILDYSKLEANKLELEITTFNLRELLDGVIQLLQRTAEGRQLRLSLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAPDQQARLFRSFAQADASTTRLYGGTGLGLAICKRIIDLMGGRIGV
ESEPGRGSTFWFEIPLLKVIGDLQQATGADAARVMVISSDARLSQRLKRLLDSWGVSHVLMETTQEALERVRRHNDSEGF
RCVIADHDTLRYSARAVHRALARPENANSARLIWLYGEEPVPAELQDHATLVPRQSPDETLRALVLPPEPKPAHVTTLAS
AMIPEPLPPPMANAREVRILLVEDNPVNLLVAQKLLAVLGFEADTATDGEAALASMESTRYDMVFMDCQMPVLDGYAATR
RWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYLSKPVAREQLDACLQRWLPRQALLPGPSSAGQATHDPESAS
AAAQARALPILDNNVIDELYEVAGADTITILQLFLEDAPLIIEQLEAAAANRDSMQLRDLAHTLKSSSANVGAQAVSNAA
RRIELAARTGTIERPSVMVALVIAEYARARLALLGQVARLQAAASTTNQ

Sequences:

>Translated_769_residues
MRERAAGLRAWSILAVLAALAAALGAGLQWSSLQGPWRAVSAVSAVLAVVCGLCAALAAHRAAQAQHALAQRLEEADLAR
ERLQQELRRHGELEQELLRAKQAAEAATLAKGEFLATMSHEIRTPLNGIIPMLELISSGQLSVDQRDMLQTATGSSLQLL
RIVDDILDYSKLEANKLELEITTFNLRELLDGVIQLLQRTAEGRQLRLSLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAPDQQARLFRSFAQADASTTRLYGGTGLGLAICKRIIDLMGGRIGV
ESEPGRGSTFWFEIPLLKVIGDLQQATGADAARVMVISSDARLSQRLKRLLDSWGVSHVLMETTQEALERVRRHNDSEGF
RCVIADHDTLRYSARAVHRALARPENANSARLIWLYGEEPVPAELQDHATLVPRQSPDETLRALVLPPEPKPAHVTTLAS
AMIPEPLPPPMANAREVRILLVEDNPVNLLVAQKLLAVLGFEADTATDGEAALASMESTRYDMVFMDCQMPVLDGYAATR
RWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYLSKPVAREQLDACLQRWLPRQALLPGPSSAGQATHDPESAS
AAAQARALPILDNNVIDELYEVAGADTITILQLFLEDAPLIIEQLEAAAANRDSMQLRDLAHTLKSSSANVGAQAVSNAA
RRIELAARTGTIERPSVMVALVIAEYARARLALLGQVARLQAAASTTNQ
>Mature_769_residues
MRERAAGLRAWSILAVLAALAAALGAGLQWSSLQGPWRAVSAVSAVLAVVCGLCAALAAHRAAQAQHALAQRLEEADLAR
ERLQQELRRHGELEQELLRAKQAAEAATLAKGEFLATMSHEIRTPLNGIIPMLELISSGQLSVDQRDMLQTATGSSLQLL
RIVDDILDYSKLEANKLELEITTFNLRELLDGVIQLLQRTAEGRQLRLSLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAPDQQARLFRSFAQADASTTRLYGGTGLGLAICKRIIDLMGGRIGV
ESEPGRGSTFWFEIPLLKVIGDLQQATGADAARVMVISSDARLSQRLKRLLDSWGVSHVLMETTQEALERVRRHNDSEGF
RCVIADHDTLRYSARAVHRALARPENANSARLIWLYGEEPVPAELQDHATLVPRQSPDETLRALVLPPEPKPAHVTTLAS
AMIPEPLPPPMANAREVRILLVEDNPVNLLVAQKLLAVLGFEADTATDGEAALASMESTRYDMVFMDCQMPVLDGYAATR
RWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYLSKPVAREQLDACLQRWLPRQALLPGPSSAGQATHDPESAS
AAAQARALPILDNNVIDELYEVAGADTITILQLFLEDAPLIIEQLEAAAANRDSMQLRDLAHTLKSSSANVGAQAVSNAA
RRIELAARTGTIERPSVMVALVIAEYARARLALLGQVARLQAAASTTNQ

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=535, Percent_Identity=33.4579439252336, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1789149, Length=289, Percent_Identity=47.7508650519031, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI87081816, Length=340, Percent_Identity=36.7647058823529, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1788713, Length=240, Percent_Identity=38.75, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI48994928, Length=255, Percent_Identity=36.8627450980392, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1786783, Length=331, Percent_Identity=26.2839879154079, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=32.258064516129, Blast_Score=100, Evalue=7e-22,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=29.8449612403101, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790436, Length=263, Percent_Identity=28.5171102661597, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=28.8065843621399, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=26.2008733624454, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=28.3333333333333, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787894, Length=242, Percent_Identity=28.9256198347107, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789808, Length=250, Percent_Identity=27.6, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=290, Percent_Identity=28.9655172413793, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790300, Length=268, Percent_Identity=28.7313432835821, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=32.8, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1789402, Length=142, Percent_Identity=32.3943661971831, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=170, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322044, Length=130, Percent_Identity=37.6923076923077, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323034, Length=184, Percent_Identity=27.1739130434783, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83692; Mature: 83692

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERAAGLRAWSILAVLAALAAALGAGLQWSSLQGPWRAVSAVSAVLAVVCGLCAALAAH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAAQAQHALAQRLEEADLARERLQQELRRHGELEQELLRAKQAAEAATLAKGEFLATMSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EIRTPLNGIIPMLELISSGQLSVDQRDMLQTATGSSLQLLRIVDDILDYSKLEANKLELE
HHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
ITTFNLRELLDGVIQLLQRTAEGRQLRLSLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
EEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHEE
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAPDQQARLFRSFAQADASTTRLYGGT
ECCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCC
GLGLAICKRIIDLMGGRIGVESEPGRGSTFWFEIPLLKVIGDLQQATGADAARVMVISSD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEECC
ARLSQRLKRLLDSWGVSHVLMETTQEALERVRRHNDSEGFRCVIADHDTLRYSARAVHRA
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
LARPENANSARLIWLYGEEPVPAELQDHATLVPRQSPDETLRALVLPPEPKPAHVTTLAS
HCCCCCCCCEEEEEEECCCCCCCCHHCCCEECCCCCCCHHHEEEEECCCCCCCHHHHHHH
AMIPEPLPPPMANAREVRILLVEDNPVNLLVAQKLLAVLGFEADTATDGEAALASMESTR
HHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
YDMVFMDCQMPVLDGYAATRRWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYL
CEEEEEECCCCHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCHHCCCHHHHHHCCCHHHH
SKPVAREQLDACLQRWLPRQALLPGPSSAGQATHDPESASAAAQARALPILDNNVIDELY
CCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
EVAGADTITILQLFLEDAPLIIEQLEAAAANRDSMQLRDLAHTLKSSSANVGAQAVSNAA
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
RRIELAARTGTIERPSVMVALVIAEYARARLALLGQVARLQAAASTTNQ
HHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRERAAGLRAWSILAVLAALAAALGAGLQWSSLQGPWRAVSAVSAVLAVVCGLCAALAAH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAAQAQHALAQRLEEADLARERLQQELRRHGELEQELLRAKQAAEAATLAKGEFLATMSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EIRTPLNGIIPMLELISSGQLSVDQRDMLQTATGSSLQLLRIVDDILDYSKLEANKLELE
HHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
ITTFNLRELLDGVIQLLQRTAEGRQLRLSLDIEPSVRLLVRGDPIRLRQVLGNLIGNAIK
EEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHEE
FTERGSVDIQLRRLGETRAQHLLRFQVRDTGIGIAPDQQARLFRSFAQADASTTRLYGGT
ECCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCC
GLGLAICKRIIDLMGGRIGVESEPGRGSTFWFEIPLLKVIGDLQQATGADAARVMVISSD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEECC
ARLSQRLKRLLDSWGVSHVLMETTQEALERVRRHNDSEGFRCVIADHDTLRYSARAVHRA
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
LARPENANSARLIWLYGEEPVPAELQDHATLVPRQSPDETLRALVLPPEPKPAHVTTLAS
HCCCCCCCCEEEEEEECCCCCCCCHHCCCEECCCCCCCHHHEEEEECCCCCCCHHHHHHH
AMIPEPLPPPMANAREVRILLVEDNPVNLLVAQKLLAVLGFEADTATDGEAALASMESTR
HHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
YDMVFMDCQMPVLDGYAATRRWRAMETESGGRPIPIVAMTANAMAGDRERCLAAGMDDYL
CEEEEEECCCCHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCHHCCCHHHHHHCCCHHHH
SKPVAREQLDACLQRWLPRQALLPGPSSAGQATHDPESASAAAQARALPILDNNVIDELY
CCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
EVAGADTITILQLFLEDAPLIIEQLEAAAANRDSMQLRDLAHTLKSSSANVGAQAVSNAA
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
RRIELAARTGTIERPSVMVALVIAEYARARLALLGQVARLQAAASTTNQ
HHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]