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The map label for this gene is glcE [H]

Identifier: 78044838

GI number: 78044838

Start: 1156588

End: 1157700

Strand: Direct

Name: glcE [H]

Synonym: CHY_1298

Alternate gene names: 78044838

Gene position: 1156588-1157700 (Clockwise)

Preceding gene: 78042639

Following gene: 78044496

Centisome position: 48.16

GC content: 30.19

Gene sequence:

>1113_bases
GTGGTGAATTTAAAGATGATGAGCGATATTTTAGAGAAAATTGAACCAGGTACTTTAGAAGAACTAATGTGGTGTATAAA
AAATTATGAAAAAACAAATAAACATATTATTCCTATAGGACAAGGAAGTACCCTTAAAACAGTCTCATTAACAAAGAGTG
ATGATTATGTGTATGTTTCTTCCAAAAATTTAAATAAAGTAATCGAATTTGCTAAAGATAATTTAACAATTACTGTTCAA
GCTGGGGCTACCCTAAAAAAAATTGATGAGTTGATTAATAAAAACAGTTTAACATTAATGCGCAGTCCTTTAATGGCAGG
GGAACGGACAATTGGAGGTATTGTTGCCGAAGGAGCATTTTTTAATAGAGATTTCAGTCAAAGCATTCTTGGTTTAAAAG
TTATTTTACCTAATGGTGATCTTATTAAAACTGGGGGAAAAACCATTAAGAACGTTAGTGGTTATGATTTAAGAAGCTTA
TTTTTTGGAAGTCGTGGGACTTTAGGTTTTTTGGTGGAAGTAACTCTTAAACTCCAGCCTGTTAAACCTGCAGCTGAATT
AATAGCTATAAAGGTTCATCCCGATGATGTTTCTACCGTTTTGATGAATATTCTTCCCTATCGTTATTATATAACCCATT
TGTTAATAGAAAATGTTAGCCTTGAAAATGAAGAGAAAGGTGAATATGTAAATATTTTGTTATTTTATGAAGGTAGCGTT
GAAGCCGTAGAATTCTTACATAAATTTATTTTGGATAAGATGATAGGAAACAAGTTTTTAAAAACAATTACAAAGACAAT
TACAGACAGGAAAGAAATTCAAAATACCATTAATAAACTTCCGGCTAATGAGTCACAATTTGCTGAATACAGAGTTTTGG
ATGAAATTGAACTGTTTAAAAATTGTGGATTTAAAAACGAAAAATTCATATTTGATATTAAGGCAAAATATTTGCGAATT
TTTAATGATACTTCTAAAGCAGAAATAGAAGTCAGTGGGAATGCTGCGAAAGTGTTGAAACATTATTTGTTTAATGACGT
TTTCCTCCAAATTAAAAAGAAAATTGATCCTAAGATGATATTTGCACCATATTTGGTGAAAATTGTGAATTGA

Upstream 100 bases:

>100_bases
GAGTTTTCACCTGAAGATCTAAATTTTATGAAACAAATTAAGGAATGTTTAGATCCAAAAGGTATTTTAAATGCTGGAAA
GGTTATACCTTAAATTGCGG

Downstream 100 bases:

>100_bases
GGTGGTAAAATGACACAAATAAAAGAATTGTTACTAAGATGTAATAAATGCGGTCTTTGTCAGCAATCGTGTCCAACATA
TAAAGTTACAAAAAATGAGT

Product: glycolate oxidase, GlcE subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MVNLKMMSDILEKIEPGTLEELMWCIKNYEKTNKHIIPIGQGSTLKTVSLTKSDDYVYVSSKNLNKVIEFAKDNLTITVQ
AGATLKKIDELINKNSLTLMRSPLMAGERTIGGIVAEGAFFNRDFSQSILGLKVILPNGDLIKTGGKTIKNVSGYDLRSL
FFGSRGTLGFLVEVTLKLQPVKPAAELIAIKVHPDDVSTVLMNILPYRYYITHLLIENVSLENEEKGEYVNILLFYEGSV
EAVEFLHKFILDKMIGNKFLKTITKTITDRKEIQNTINKLPANESQFAEYRVLDEIELFKNCGFKNEKFIFDIKAKYLRI
FNDTSKAEIEVSGNAAKVLKHYLFNDVFLQIKKKIDPKMIFAPYLVKIVN

Sequences:

>Translated_370_residues
MVNLKMMSDILEKIEPGTLEELMWCIKNYEKTNKHIIPIGQGSTLKTVSLTKSDDYVYVSSKNLNKVIEFAKDNLTITVQ
AGATLKKIDELINKNSLTLMRSPLMAGERTIGGIVAEGAFFNRDFSQSILGLKVILPNGDLIKTGGKTIKNVSGYDLRSL
FFGSRGTLGFLVEVTLKLQPVKPAAELIAIKVHPDDVSTVLMNILPYRYYITHLLIENVSLENEEKGEYVNILLFYEGSV
EAVEFLHKFILDKMIGNKFLKTITKTITDRKEIQNTINKLPANESQFAEYRVLDEIELFKNCGFKNEKFIFDIKAKYLRI
FNDTSKAEIEVSGNAAKVLKHYLFNDVFLQIKKKIDPKMIFAPYLVKIVN
>Mature_370_residues
MVNLKMMSDILEKIEPGTLEELMWCIKNYEKTNKHIIPIGQGSTLKTVSLTKSDDYVYVSSKNLNKVIEFAKDNLTITVQ
AGATLKKIDELINKNSLTLMRSPLMAGERTIGGIVAEGAFFNRDFSQSILGLKVILPNGDLIKTGGKTIKNVSGYDLRSL
FFGSRGTLGFLVEVTLKLQPVKPAAELIAIKVHPDDVSTVLMNILPYRYYITHLLIENVSLENEEKGEYVNILLFYEGSV
EAVEFLHKFILDKMIGNKFLKTITKTITDRKEIQNTINKLPANESQFAEYRVLDEIELFKNCGFKNEKFIFDIKAKYLRI
FNDTSKAEIEVSGNAAKVLKHYLFNDVFLQIKKKIDPKMIFAPYLVKIVN

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=167, Percent_Identity=28.7425149700599, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI119964728, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI48994914, Length=127, Percent_Identity=36.2204724409449, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556096, Length=194, Percent_Identity=28.3505154639175, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320027, Length=182, Percent_Identity=32.967032967033, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320023, Length=178, Percent_Identity=29.2134831460674, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320764, Length=158, Percent_Identity=31.0126582278481, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 41967; Mature: 41967

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLKMMSDILEKIEPGTLEELMWCIKNYEKTNKHIIPIGQGSTLKTVSLTKSDDYVYVS
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCEEEEE
SKNLNKVIEFAKDNLTITVQAGATLKKIDELINKNSLTLMRSPLMAGERTIGGIVAEGAF
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEHHCCCCCCCCHHCCEEECCCC
FNRDFSQSILGLKVILPNGDLIKTGGKTIKNVSGYDLRSLFFGSRGTLGFLVEVTLKLQP
CCCCHHHCCCEEEEEECCCCEEECCCCHHCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEC
VKPAAELIAIKVHPDDVSTVLMNILPYRYYITHLLIENVSLENEEKGEYVNILLFYEGSV
CCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCH
EAVEFLHKFILDKMIGNKFLKTITKTITDRKEIQNTINKLPANESQFAEYRVLDEIELFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NCGFKNEKFIFDIKAKYLRIFNDTSKAEIEVSGNAAKVLKHYLFNDVFLQIKKKIDPKMI
HCCCCCCEEEEEEHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEE
FAPYLVKIVN
HHHHHHHCCC
>Mature Secondary Structure
MVNLKMMSDILEKIEPGTLEELMWCIKNYEKTNKHIIPIGQGSTLKTVSLTKSDDYVYVS
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCEEEEE
SKNLNKVIEFAKDNLTITVQAGATLKKIDELINKNSLTLMRSPLMAGERTIGGIVAEGAF
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEHHCCCCCCCCHHCCEEECCCC
FNRDFSQSILGLKVILPNGDLIKTGGKTIKNVSGYDLRSLFFGSRGTLGFLVEVTLKLQP
CCCCHHHCCCEEEEEECCCCEEECCCCHHCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEC
VKPAAELIAIKVHPDDVSTVLMNILPYRYYITHLLIENVSLENEEKGEYVNILLFYEGSV
CCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCH
EAVEFLHKFILDKMIGNKFLKTITKTITDRKEIQNTINKLPANESQFAEYRVLDEIELFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NCGFKNEKFIFDIKAKYLRIFNDTSKAEIEVSGNAAKVLKHYLFNDVFLQIKKKIDPKMI
HCCCCCCEEEEEEHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEE
FAPYLVKIVN
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]