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The map label for this gene is glcD [H]

Identifier: 78042639

GI number: 78042639

Start: 1155204

End: 1156580

Strand: Direct

Name: glcD [H]

Synonym: CHY_1297

Alternate gene names: 78042639

Gene position: 1155204-1156580 (Clockwise)

Preceding gene: 78043540

Following gene: 78044838

Centisome position: 48.1

GC content: 36.96

Gene sequence:

>1377_bases
GTGCTTTCCAAAAAAATCGTTGAAGAATTGTGGGAAATAGTAGGAAAAGAAAATGTTATTACTGATCCTATAGTTTTAGA
AGTTTACGGAATTGATGCTTCACCGTATAGTTCAATACCCAAAGCAGTGATTTTTCCGGAGAATACTGAACAAATTATTA
AATTAGTAAAGCTTGCCAGTCGCGAAGACCTGCCTATAATACCTAGGGGAGCCGGAACATCGCTTTGTGGTGGTGTGGTT
CCTGTAAAAAGTGATATTATTCTTGTCCTAACTAAAATGAAAGAAGTTATCGAAATAAATAAAAAGGATGGATATGCTGT
TGTTGAACCTGGACTTACCAATGGCGAGTTACAAGAAATATTAAAGCCTTATGGTTTTATGTTTGCACCTGATCCATCAA
GTTTTAGTGTCTCAACTATAGGAGGTAACGTTGGGGCAAATGCCGGTGGTATTAAAGGGGTTAAATATGGGGTAACAAGC
AATCACCTTTTAGGCCTGGAGGTAGTTATGCCTGATGGAGAACTTATTAAGACAGGAATTTTATCACCTAACTATGGGGT
TGAGCATGACATCACAGGCCTATTTTGTGGTTCGGAAGGAACATTTGGGATTATAACTAAAATTGCTGTGAAATTAACAC
CTTTGCCACAAAGTATAGGTACTCTTTTAACCTTTTTTACTTCATTGCATGATGCTGGACAAGCAGTTTCTGATATTATT
GCTGAGGGGATTATTCCAACTACATTAGAAATAATGGATAAAATTACAGCTAAAGCGGTAAATGAGTATATAAATTTAGG
ATTGAGACCAGAAACTGAAGCCTTGCTTCTTATTGAAGTAGATGGCTTAGAAGCTGAAATTCCTGGAACATTAGCTCGTA
TAGAAAGAATCTTACATAAAAACAACTGTATAAGTGTTTCGAAAGCGCAAACGCCAGAGGAAAGAGAATTACTTTGGCGG
GCACGCCGTTCAAATGCTGGAGCAATGGGTAGGATTAGGCCTTTAAATATAACTCAGGATATTGTGGTTCCCCGTGATAA
GTTACCGGAAATGATTAGTACCACCCAAGAAATTGCAAAAAAATTTAATGTTCTTATTGGCCAAGTAGCACATGCTGGAG
ACGGCAACGTTCATCCAATATTTTTATACTATCCTTGGGACCATGATGAATTAGAACGGGTAGAAAAAGCTTGTGACGAG
GTTATCAAATTAGCAATTGATTTGGGAGGAACGATCTCAGGTGAACATGGTATCGGGATTGAAAAATTAAAGTATATGAG
TTGGGAGTTTTCACCTGAAGATCTAAATTTTATGAAACAAATTAAGGAATGTTTAGATCCAAAAGGTATTTTAAATGCTG
GAAAGGTTATACCTTAA

Upstream 100 bases:

>100_bases
TTTTAAAAATTCGTAAACCTGGTATTGTTTTTGCTTAAGTGTTATTGTGTGTAAAAGCCGGCCGGAGCGGAAATTTATAT
TTGGTTTGGGAGTGGTAAAA

Downstream 100 bases:

>100_bases
ATTGCGGGTGGTGAATTTAAAGATGATGAGCGATATTTTAGAGAAAATTGAACCAGGTACTTTAGAAGAACTAATGTGGT
GTATAAAAAATTATGAAAAA

Product: glycolate oxidase, GlcD subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLSKKIVEELWEIVGKENVITDPIVLEVYGIDASPYSSIPKAVIFPENTEQIIKLVKLASREDLPIIPRGAGTSLCGGVV
PVKSDIILVLTKMKEVIEINKKDGYAVVEPGLTNGELQEILKPYGFMFAPDPSSFSVSTIGGNVGANAGGIKGVKYGVTS
NHLLGLEVVMPDGELIKTGILSPNYGVEHDITGLFCGSEGTFGIITKIAVKLTPLPQSIGTLLTFFTSLHDAGQAVSDII
AEGIIPTTLEIMDKITAKAVNEYINLGLRPETEALLLIEVDGLEAEIPGTLARIERILHKNNCISVSKAQTPEERELLWR
ARRSNAGAMGRIRPLNITQDIVVPRDKLPEMISTTQEIAKKFNVLIGQVAHAGDGNVHPIFLYYPWDHDELERVEKACDE
VIKLAIDLGGTISGEHGIGIEKLKYMSWEFSPEDLNFMKQIKECLDPKGILNAGKVIP

Sequences:

>Translated_458_residues
MLSKKIVEELWEIVGKENVITDPIVLEVYGIDASPYSSIPKAVIFPENTEQIIKLVKLASREDLPIIPRGAGTSLCGGVV
PVKSDIILVLTKMKEVIEINKKDGYAVVEPGLTNGELQEILKPYGFMFAPDPSSFSVSTIGGNVGANAGGIKGVKYGVTS
NHLLGLEVVMPDGELIKTGILSPNYGVEHDITGLFCGSEGTFGIITKIAVKLTPLPQSIGTLLTFFTSLHDAGQAVSDII
AEGIIPTTLEIMDKITAKAVNEYINLGLRPETEALLLIEVDGLEAEIPGTLARIERILHKNNCISVSKAQTPEERELLWR
ARRSNAGAMGRIRPLNITQDIVVPRDKLPEMISTTQEIAKKFNVLIGQVAHAGDGNVHPIFLYYPWDHDELERVEKACDE
VIKLAIDLGGTISGEHGIGIEKLKYMSWEFSPEDLNFMKQIKECLDPKGILNAGKVIP
>Mature_458_residues
MLSKKIVEELWEIVGKENVITDPIVLEVYGIDASPYSSIPKAVIFPENTEQIIKLVKLASREDLPIIPRGAGTSLCGGVV
PVKSDIILVLTKMKEVIEINKKDGYAVVEPGLTNGELQEILKPYGFMFAPDPSSFSVSTIGGNVGANAGGIKGVKYGVTS
NHLLGLEVVMPDGELIKTGILSPNYGVEHDITGLFCGSEGTFGIITKIAVKLTPLPQSIGTLLTFFTSLHDAGQAVSDII
AEGIIPTTLEIMDKITAKAVNEYINLGLRPETEALLLIEVDGLEAEIPGTLARIERILHKNNCISVSKAQTPEERELLWR
ARRSNAGAMGRIRPLNITQDIVVPRDKLPEMISTTQEIAKKFNVLIGQVAHAGDGNVHPIFLYYPWDHDELERVEKACDE
VIKLAIDLGGTISGEHGIGIEKLKYMSWEFSPEDLNFMKQIKECLDPKGILNAGKVIP

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=464, Percent_Identity=34.698275862069, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI37595754, Length=485, Percent_Identity=32.9896907216495, Blast_Score=261, Evalue=9e-70,
Organism=Homo sapiens, GI119964728, Length=450, Percent_Identity=28, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4501993, Length=264, Percent_Identity=27.6515151515151, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1789351, Length=428, Percent_Identity=38.0841121495327, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1787977, Length=502, Percent_Identity=24.9003984063745, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI48994907, Length=483, Percent_Identity=21.9461697722567, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=31.3852813852814, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=30.7359307359307, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI71992392, Length=366, Percent_Identity=31.9672131147541, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17534361, Length=426, Percent_Identity=28.4037558685446, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17556096, Length=477, Percent_Identity=21.3836477987421, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320027, Length=430, Percent_Identity=29.0697674418605, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320023, Length=443, Percent_Identity=25.7336343115124, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320764, Length=445, Percent_Identity=25.8426966292135, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI18921117, Length=472, Percent_Identity=26.9067796610169, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24639277, Length=472, Percent_Identity=26.9067796610169, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24639275, Length=472, Percent_Identity=26.9067796610169, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24653753, Length=482, Percent_Identity=21.3692946058091, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49835; Mature: 49835

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKKIVEELWEIVGKENVITDPIVLEVYGIDASPYSSIPKAVIFPENTEQIIKLVKLAS
CCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHCCCEEECCCCHHHHHHHHHHHC
REDLPIIPRGAGTSLCGGVVPVKSDIILVLTKMKEVIEINKKDGYAVVEPGLTNGELQEI
CCCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
LKPYGFMFAPDPSSFSVSTIGGNVGANAGGIKGVKYGVTSNHLLGLEVVMPDGELIKTGI
HHHHCEEECCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEECC
LSPNYGVEHDITGLFCGSEGTFGIITKIAVKLTPLPQSIGTLLTFFTSLHDAGQAVSDII
CCCCCCCCCCCCEEEECCCCCHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEGIIPTTLEIMDKITAKAVNEYINLGLRPETEALLLIEVDGLEAEIPGTLARIERILHK
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHC
NNCISVSKAQTPEERELLWRARRSNAGAMGRIRPLNITQDIVVPRDKLPEMISTTQEIAK
CCCEEECCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KFNVLIGQVAHAGDGNVHPIFLYYPWDHDELERVEKACDEVIKLAIDLGGTISGEHGIGI
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCH
EKLKYMSWEFSPEDLNFMKQIKECLDPKGILNAGKVIP
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSKKIVEELWEIVGKENVITDPIVLEVYGIDASPYSSIPKAVIFPENTEQIIKLVKLAS
CCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHCCCEEECCCCHHHHHHHHHHHC
REDLPIIPRGAGTSLCGGVVPVKSDIILVLTKMKEVIEINKKDGYAVVEPGLTNGELQEI
CCCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
LKPYGFMFAPDPSSFSVSTIGGNVGANAGGIKGVKYGVTSNHLLGLEVVMPDGELIKTGI
HHHHCEEECCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEECC
LSPNYGVEHDITGLFCGSEGTFGIITKIAVKLTPLPQSIGTLLTFFTSLHDAGQAVSDII
CCCCCCCCCCCCEEEECCCCCHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEGIIPTTLEIMDKITAKAVNEYINLGLRPETEALLLIEVDGLEAEIPGTLARIERILHK
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHC
NNCISVSKAQTPEERELLWRARRSNAGAMGRIRPLNITQDIVVPRDKLPEMISTTQEIAK
CCCEEECCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KFNVLIGQVAHAGDGNVHPIFLYYPWDHDELERVEKACDEVIKLAIDLGGTISGEHGIGI
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCH
EKLKYMSWEFSPEDLNFMKQIKECLDPKGILNAGKVIP
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]