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The map label for this gene is glcF [H]

Identifier: 78044496

GI number: 78044496

Start: 1157710

End: 1158948

Strand: Direct

Name: glcF [H]

Synonym: CHY_1299

Alternate gene names: 78044496

Gene position: 1157710-1158948 (Clockwise)

Preceding gene: 78044838

Following gene: 78043354

Centisome position: 48.21

GC content: 30.99

Gene sequence:

>1239_bases
ATGACACAAATAAAAGAATTGTTACTAAGATGTAATAAATGCGGTCTTTGTCAGCAATCGTGTCCAACATATAAAGTTAC
AAAAAATGAGTTTTACTTAGCTCGTGGGCGGAATCGATTAATGGACTTAGTACTAGAAAAAAAATATGACTGGCAAAAAC
AAATAATATATGCAATAGAAACTTGTTTACAGTGTGGAGCGTGTGTTGAGACCTGTCCATCTTCGGTAAAAACTGACTTA
GTTATGAATTTGGCGAAAAGAGAAATAGTAAAAATAAAGAAACAAAATATCTTATTAAAATTTGCCTACAAAATTTTGTT
GCCTACTCCTCAAATGATAAAAATTGTAGGTAAATTTGTCAAGGTATATTCAAAAGTAAAAGATAAAAAAGTTGTTAAAT
ATGTTAAAAGATTAAATCCTATTAATGACCTTAAAAGGTTAGAGAGGTCTTTGCCTAATTATCGAGGGGAAGTAGGTGAG
TTAAAAACAAAGGGAAAGGGAAGTTTTAATAAAGAGATAAGAGTTGGCTATTTTAAAAATTGTGCTAATCTTCTTGCCTT
TAATTTTTTAGATCAATCAACTTTTAATGTGCTGAAAGCAGTAGGAATTCATGTGGAGAATTTAACAAATAAATGTTGTG
GAGCTCCTCATTGGTTTAGTGGGGATATAGAAACATTTAGGAATTTAGCAAATTACAATTTAAAAAATAGTGAATACCAA
AAATATGATTTTATTGTGACAGATTGCGCTACCTGTGGGGCAATATTAAAAAGATATGGAGAATTATTGGAAACGAAAGA
AGCGCACGAATTTGCCCAAAAGGTAATAGATATTAATGAGTTTTTAGTAAAAAATGAGTTGCTTGATTATTTTGAATATG
AAGAGAGGAGTGATGTTATAACAGTTCATGATCCATGTCATTTAGTGCGGGAGCAAAAGGTAAAATTTGAGCCTAGAAAA
ATTGCGAATATGGTGCCTGGGGTAAAACTAATTGAAATGAAAGACTCGAATATGTGCTGTGGAGGAGCTGGGGTCTTTAG
CATAACACAGCCAAGGATGTCTGATGAAATTTTGAAAGTAAAAATTAAAAATATATTAGAAACAAAGGCTAATATAGTTT
TAGCTGCCTGTCCTTCTTGTATTATGCAATTAACGCGGGGTGTAGAAACTTTTGCTCCTAATAGTAATATAAAAGTATTT
CATCCAATTGAATATTTAGTTCAACATATAAAAAAATAA

Upstream 100 bases:

>100_bases
TTATTTGTTTAATGACGTTTTCCTCCAAATTAAAAAGAAAATTGATCCTAAGATGATATTTGCACCATATTTGGTGAAAA
TTGTGAATTGAGGTGGTAAA

Downstream 100 bases:

>100_bases
ATTAGTTAATTATTTGGAGGAGGTGGCCTTTTTAAGAAAAATTGAGTAAAGTTCTAAAAGAATACTTAAGAGCGATAGGG
GGTGAAAGTAAAAAAGTTTG

Product: glycolate oxidase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTQIKELLLRCNKCGLCQQSCPTYKVTKNEFYLARGRNRLMDLVLEKKYDWQKQIIYAIETCLQCGACVETCPSSVKTDL
VMNLAKREIVKIKKQNILLKFAYKILLPTPQMIKIVGKFVKVYSKVKDKKVVKYVKRLNPINDLKRLERSLPNYRGEVGE
LKTKGKGSFNKEIRVGYFKNCANLLAFNFLDQSTFNVLKAVGIHVENLTNKCCGAPHWFSGDIETFRNLANYNLKNSEYQ
KYDFIVTDCATCGAILKRYGELLETKEAHEFAQKVIDINEFLVKNELLDYFEYEERSDVITVHDPCHLVREQKVKFEPRK
IANMVPGVKLIEMKDSNMCCGGAGVFSITQPRMSDEILKVKIKNILETKANIVLAACPSCIMQLTRGVETFAPNSNIKVF
HPIEYLVQHIKK

Sequences:

>Translated_412_residues
MTQIKELLLRCNKCGLCQQSCPTYKVTKNEFYLARGRNRLMDLVLEKKYDWQKQIIYAIETCLQCGACVETCPSSVKTDL
VMNLAKREIVKIKKQNILLKFAYKILLPTPQMIKIVGKFVKVYSKVKDKKVVKYVKRLNPINDLKRLERSLPNYRGEVGE
LKTKGKGSFNKEIRVGYFKNCANLLAFNFLDQSTFNVLKAVGIHVENLTNKCCGAPHWFSGDIETFRNLANYNLKNSEYQ
KYDFIVTDCATCGAILKRYGELLETKEAHEFAQKVIDINEFLVKNELLDYFEYEERSDVITVHDPCHLVREQKVKFEPRK
IANMVPGVKLIEMKDSNMCCGGAGVFSITQPRMSDEILKVKIKNILETKANIVLAACPSCIMQLTRGVETFAPNSNIKVF
HPIEYLVQHIKK
>Mature_411_residues
TQIKELLLRCNKCGLCQQSCPTYKVTKNEFYLARGRNRLMDLVLEKKYDWQKQIIYAIETCLQCGACVETCPSSVKTDLV
MNLAKREIVKIKKQNILLKFAYKILLPTPQMIKIVGKFVKVYSKVKDKKVVKYVKRLNPINDLKRLERSLPNYRGEVGEL
KTKGKGSFNKEIRVGYFKNCANLLAFNFLDQSTFNVLKAVGIHVENLTNKCCGAPHWFSGDIETFRNLANYNLKNSEYQK
YDFIVTDCATCGAILKRYGELLETKEAHEFAQKVIDINEFLVKNELLDYFEYEERSDVITVHDPCHLVREQKVKFEPRKI
ANMVPGVKLIEMKDSNMCCGGAGVFSITQPRMSDEILKVKIKNILETKANIVLAACPSCIMQLTRGVETFAPNSNIKVFH
PIEYLVQHIKK

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=424, Percent_Identity=24.7641509433962, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786497, Length=246, Percent_Identity=25.2032520325203, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 47342; Mature: 47211

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCEEEHHHHHHHHHHHHCHHHCCCCCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure 
TQIKELLLRCNKCGLCQQSCPTYKVTKNEFYLARGRNRLMDLVLEKKYDWQKQIIYAIE
CHHHHHHHHCCCCCCCHHCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHH
TCLQCGACVETCPSSVKTDLVMNLAKREIVKIKKQNILLKFAYKILLPTPQMIKIVGKFV
HHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KVYSKVKDKKVVKYVKRLNPINDLKRLERSLPNYRGEVGELKTKGKGSFNKEIRVGYFKN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEHHHHHH
CANLLAFNFLDQSTFNVLKAVGIHVENLTNKCCGAPHWFSGDIETFRNLANYNLKNSEYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCC
KYDFIVTDCATCGAILKRYGELLETKEAHEFAQKVIDINEFLVKNELLDYFEYEERSDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TVHDPCHLVREQKVKFEPRKIANMVPGVKLIEMKDSNMCCGGAGVFSITQPRMSDEILKV
EECCHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCEECCCCEEEECCCCHHHHHHHH
KIKNILETKANIVLAACPSCIMQLTRGVETFAPNSNIKVFHPIEYLVQHIKK
HHHHHHHHHHCEEEHHHHHHHHHHHHCHHHCCCCCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]