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The map label for this gene is tpiA

Identifier: 78044231

GI number: 78044231

Start: 248431

End: 249186

Strand: Direct

Name: tpiA

Synonym: CHY_0282

Alternate gene names: 78044231

Gene position: 248431-249186 (Clockwise)

Preceding gene: 78044457

Following gene: 78042968

Centisome position: 10.34

GC content: 48.02

Gene sequence:

>756_bases
ATGAGAAAACCTTTTATTGCTGGCAACTGGAAAATGTATAAAACCCCTGTCGAGGCGGCCGGATTTGTCCGTGAGCTCAT
CGATAGCTTAAAAGATGTTAGCGGTGTGGACGTAGCGGTCTGTCCGCCTTTTCCTGCCCTGTGGCCGGTTAAGGAAGCAT
TAGAGGGGAGCAATATTGCTCTGGGAGCCCAGAACATGCATTTTGAAAAGGAAGGGGCTTTTACCGGTGAAGTATCTCCG
GCCATGCTTCAAGATATAGGGGTTAAGTACGTAATCTTGGGACACTCCGAGCGGAGAGCTTACTTTGGGGAAACGGACGA
ACTTATTAACCAGAAAATAAAGGCAGCTTTTACCTGGGGACTAAATCCTATCTTTTGCGTTGGCGAAACTTTGGAAGAGC
GGGAACGGGGAATTACCAAGGCAGTAGTGGAAATTCAGGTGTTAAAGGGCCTGGCTGGAGTTACGGCAGAGCAGGCCGAA
AACCTGACCATTGCTTACGAGCCGGTATGGGCAATTGGTACCGGAAAAACCGCAACTCCCGATGATGCTCAGGAAGTGTG
CCAGTTTATCCGGGAACTTTTGGTAAAGCTTTTCGGGCGGGAGATAGCGGATAAAGTTCGCATTCAGTACGGTGGTAGTG
TTAAGCCGGAAAATATTAAAGAGCTTATTGCCAAACCGGATATCGACGGTGCCTTAGTAGGCGGTGCCAGCTTAAAAGTA
GAAAGCTTTACCGCAATAGTTAAAGGCAGTATTTAA

Upstream 100 bases:

>100_bases
CATATTTCCACCGGCGGGGGAGCATCTCTGGAATTTTTAGAAGGGAAAAAACTTCCCGGAGTTGAAGTTTTAAACGATAA
GTAATTACGGGGGGCGGGGT

Downstream 100 bases:

>100_bases
GGAGGACTTAAAGTGTCTGTATTACTGACAATATTAGACGGCTGGGGTATAGCGGAAACTACCTATGGCAACAGTATAAA
AGAGGCGGGAACTCCCAATT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRKPFIAGNWKMYKTPVEAAGFVRELIDSLKDVSGVDVAVCPPFPALWPVKEALEGSNIALGAQNMHFEKEGAFTGEVSP
AMLQDIGVKYVILGHSERRAYFGETDELINQKIKAAFTWGLNPIFCVGETLEERERGITKAVVEIQVLKGLAGVTAEQAE
NLTIAYEPVWAIGTGKTATPDDAQEVCQFIRELLVKLFGREIADKVRIQYGGSVKPENIKELIAKPDIDGALVGGASLKV
ESFTAIVKGSI

Sequences:

>Translated_251_residues
MRKPFIAGNWKMYKTPVEAAGFVRELIDSLKDVSGVDVAVCPPFPALWPVKEALEGSNIALGAQNMHFEKEGAFTGEVSP
AMLQDIGVKYVILGHSERRAYFGETDELINQKIKAAFTWGLNPIFCVGETLEERERGITKAVVEIQVLKGLAGVTAEQAE
NLTIAYEPVWAIGTGKTATPDDAQEVCQFIRELLVKLFGREIADKVRIQYGGSVKPENIKELIAKPDIDGALVGGASLKV
ESFTAIVKGSI
>Mature_251_residues
MRKPFIAGNWKMYKTPVEAAGFVRELIDSLKDVSGVDVAVCPPFPALWPVKEALEGSNIALGAQNMHFEKEGAFTGEVSP
AMLQDIGVKYVILGHSERRAYFGETDELINQKIKAAFTWGLNPIFCVGETLEERERGITKAVVEIQVLKGLAGVTAEQAE
NLTIAYEPVWAIGTGKTATPDDAQEVCQFIRELLVKLFGREIADKVRIQYGGSVKPENIKELIAKPDIDGALVGGASLKV
ESFTAIVKGSI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=243, Percent_Identity=46.0905349794239, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI226529917, Length=243, Percent_Identity=46.0905349794239, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=50, Blast_Score=231, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17536593, Length=242, Percent_Identity=50, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6320255, Length=243, Percent_Identity=43.2098765432099, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28572008, Length=240, Percent_Identity=49.1666666666667, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28572006, Length=240, Percent_Identity=49.1666666666667, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28572004, Length=240, Percent_Identity=49.1666666666667, Blast_Score=208, Evalue=3e-54,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CARHZ (Q3AFD0)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359154.1
- HSSP:   P36204
- ProteinModelPortal:   Q3AFD0
- SMR:   Q3AFD0
- STRING:   Q3AFD0
- GeneID:   3727348
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0282
- NMPDR:   fig|246194.3.peg.293
- TIGR:   CHY_0282
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- BioCyc:   CHYD246194:CHY_0282-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27294; Mature: 27294

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPFIAGNWKMYKTPVEAAGFVRELIDSLKDVSGVDVAVCPPFPALWPVKEALEGSNIA
CCCCEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHCCCCCEE
LGAQNMHFEKEGAFTGEVSPAMLQDIGVKYVILGHSERRAYFGETDELINQKIKAAFTWG
ECCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
LNPIFCVGETLEERERGITKAVVEIQVLKGLAGVTAEQAENLTIAYEPVWAIGTGKTATP
CCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCEEEECCCCCCCC
DDAQEVCQFIRELLVKLFGREIADKVRIQYGGSVKPENIKELIAKPDIDGALVGGASLKV
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCCEEECCCEEEH
ESFTAIVKGSI
HHHHEEEECCC
>Mature Secondary Structure
MRKPFIAGNWKMYKTPVEAAGFVRELIDSLKDVSGVDVAVCPPFPALWPVKEALEGSNIA
CCCCEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHCCCCCEE
LGAQNMHFEKEGAFTGEVSPAMLQDIGVKYVILGHSERRAYFGETDELINQKIKAAFTWG
ECCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
LNPIFCVGETLEERERGITKAVVEIQVLKGLAGVTAEQAENLTIAYEPVWAIGTGKTATP
CCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCEEEECCCCCCCC
DDAQEVCQFIRELLVKLFGREIADKVRIQYGGSVKPENIKELIAKPDIDGALVGGASLKV
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCCEEECCCEEEH
ESFTAIVKGSI
HHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA