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The map label for this gene is pgk

Identifier: 78044457

GI number: 78044457

Start: 247230

End: 248414

Strand: Direct

Name: pgk

Synonym: CHY_0281

Alternate gene names: 78044457

Gene position: 247230-248414 (Clockwise)

Preceding gene: 78043763

Following gene: 78044231

Centisome position: 10.29

GC content: 47.0

Gene sequence:

>1185_bases
ATGAACAAAAAGTCTATAAGAGATGTGGAGGTAAAAGGCAAAAAAGTTTTTGTGCGGGTTGATTTTAACGTACCTTTAAA
TGAGAACCGGGAAATAACCGATGATACTCGCATCCGGGCAGCCCTTCCTACCCTCCAGTACTTGAGAGAACAAGGGGCTA
AACTTATCGTCGCCTCTCATTTAGGCCGACCCAAAGGTCAATTTAACGAAAAATACAGCTTAAAACCGGTTGCTAAAAGA
TTGGCCGAGCTTCTTGGTATAGAGGTTAAAATGGCTCCCGATGTAGTGGGGCCTGAGGTGGAAAAAATGGCCGGGGAATT
ACGGCCGGGAGAAGTACTTCTTTTAGAAAACGTACGCTTTTATCCCGAAGAGGAAAAAAATGATCCGGAATTTGCCCGAA
AACTGGCGCGGCTTGCCGAAATTTTTGTCAGCGATGCCTTTGGGGCTGCTCACCGGGCTCATGCTTCTACCGCCGGTATA
GCATCGTATTTACCGGCTGTAGCCGGATTTTTAATGGAAAAAGAAATTAACTTCCTTTCCCGGGCATTAAATAACCCGGA
ACGGCCCTTTGTGGCTATTATTGGTGGAGCCAAGGTTTCCGATAAAATCGGGGTGATTGAAAATCTTCTCGGCAAAGTTG
ACGTTCTTTTAATTGGCGGTGGCATGGCCAACACCTTTTTAAAAGCTATGGGCTATGAAACCGGAAAATCTTTGGTGGAA
GAGGATAAAGTAAATTTAGCCGGGGAGTTAATGAATAAAGCAAAAGAAGTAGGAGTTAAACTTTTGCTGCCGAGTGACGT
GGTGGTGGCTCCCAGGATTGAAGCGGGCGTACCCAGCAAAATTGTAGCGGTTGACAGCATTCCTGCTGAAGAAATGGCTT
TGGATATTGGCGAAAAAACCGCAAAGCATTTCCGGGAAGAGATTTTAAAAGCTAAGACCGTTGTCTGGAACGGGCCGATG
GGGGTTTTTGAAGTGGAACCCTTTGACCGGGGTACTGTTGCGGTAGCCGAAGCGGTAGCCGAAAGCGGCGCTCTTTCCGT
GGTCGGCGGTGGAGACTCGGTTGCGGCGGTAGAAAAGGCCGGAGTTGCGGACAAAATAACCCATATTTCCACCGGCGGGG
GAGCATCTCTGGAATTTTTAGAAGGGAAAAAACTTCCCGGAGTTGAAGTTTTAAACGATAAGTAA

Upstream 100 bases:

>100_bases
AACCGTCTGGTGGACTTAGCGACTTACATTGCCAAAAAAGGTTTATAATAGTGGGGGCCAAGTCCCCCGTTTCCCATTTT
AAGAAGGGAAGGGGAGTGTT

Downstream 100 bases:

>100_bases
TTACGGGGGGCGGGGTATGAGAAAACCTTTTATTGCTGGCAACTGGAAAATGTATAAAACCCCTGTCGAGGCGGCCGGAT
TTGTCCGTGAGCTCATCGAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKSIRDVEVKGKKVFVRVDFNVPLNENREITDDTRIRAALPTLQYLREQGAKLIVASHLGRPKGQFNEKYSLKPVAKR
LAELLGIEVKMAPDVVGPEVEKMAGELRPGEVLLLENVRFYPEEEKNDPEFARKLARLAEIFVSDAFGAAHRAHASTAGI
ASYLPAVAGFLMEKEINFLSRALNNPERPFVAIIGGAKVSDKIGVIENLLGKVDVLLIGGGMANTFLKAMGYETGKSLVE
EDKVNLAGELMNKAKEVGVKLLLPSDVVVAPRIEAGVPSKIVAVDSIPAEEMALDIGEKTAKHFREEILKAKTVVWNGPM
GVFEVEPFDRGTVAVAEAVAESGALSVVGGGDSVAAVEKAGVADKITHISTGGGASLEFLEGKKLPGVEVLNDK

Sequences:

>Translated_394_residues
MNKKSIRDVEVKGKKVFVRVDFNVPLNENREITDDTRIRAALPTLQYLREQGAKLIVASHLGRPKGQFNEKYSLKPVAKR
LAELLGIEVKMAPDVVGPEVEKMAGELRPGEVLLLENVRFYPEEEKNDPEFARKLARLAEIFVSDAFGAAHRAHASTAGI
ASYLPAVAGFLMEKEINFLSRALNNPERPFVAIIGGAKVSDKIGVIENLLGKVDVLLIGGGMANTFLKAMGYETGKSLVE
EDKVNLAGELMNKAKEVGVKLLLPSDVVVAPRIEAGVPSKIVAVDSIPAEEMALDIGEKTAKHFREEILKAKTVVWNGPM
GVFEVEPFDRGTVAVAEAVAESGALSVVGGGDSVAAVEKAGVADKITHISTGGGASLEFLEGKKLPGVEVLNDK
>Mature_394_residues
MNKKSIRDVEVKGKKVFVRVDFNVPLNENREITDDTRIRAALPTLQYLREQGAKLIVASHLGRPKGQFNEKYSLKPVAKR
LAELLGIEVKMAPDVVGPEVEKMAGELRPGEVLLLENVRFYPEEEKNDPEFARKLARLAEIFVSDAFGAAHRAHASTAGI
ASYLPAVAGFLMEKEINFLSRALNNPERPFVAIIGGAKVSDKIGVIENLLGKVDVLLIGGGMANTFLKAMGYETGKSLVE
EDKVNLAGELMNKAKEVGVKLLLPSDVVVAPRIEAGVPSKIVAVDSIPAEEMALDIGEKTAKHFREEILKAKTVVWNGPM
GVFEVEPFDRGTVAVAEAVAESGALSVVGGGDSVAAVEKAGVADKITHISTGGGASLEFLEGKKLPGVEVLNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=46.9879518072289, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI31543397, Length=414, Percent_Identity=44.2028985507246, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=45.3400503778337, Blast_Score=334, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=46.2469733656174, Blast_Score=354, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=48.792270531401, Blast_Score=358, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=47.3429951690821, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=45.3883495145631, Blast_Score=323, Evalue=8e-89,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CARHZ (Q3AFD1)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359153.1
- ProteinModelPortal:   Q3AFD1
- SMR:   Q3AFD1
- STRING:   Q3AFD1
- GeneID:   3727576
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0281
- NMPDR:   fig|246194.3.peg.292
- TIGR:   CHY_0281
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   CHYD246194:CHY_0281-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42295; Mature: 42295

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 119-119 BINDING 152-152 BINDING 202-202 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSIRDVEVKGKKVFVRVDFNVPLNENREITDDTRIRAALPTLQYLREQGAKLIVASH
CCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHH
LGRPKGQFNEKYSLKPVAKRLAELLGIEVKMAPDVVGPEVEKMAGELRPGEVLLLENVRF
CCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCCCEEEEECCEE
YPEEEKNDPEFARKLARLAEIFVSDAFGAAHRAHASTAGIASYLPAVAGFLMEKEINFLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALNNPERPFVAIIGGAKVSDKIGVIENLLGKVDVLLIGGGMANTFLKAMGYETGKSLVE
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCHHHHHHHH
EDKVNLAGELMNKAKEVGVKLLLPSDVVVAPRIEAGVPSKIVAVDSIPAEEMALDIGEKT
HHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHH
AKHFREEILKAKTVVWNGPMGVFEVEPFDRGTVAVAEAVAESGALSVVGGGDSVAAVEKA
HHHHHHHHHHHHEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHC
GVADKITHISTGGGASLEFLEGKKLPGVEVLNDK
CCHHHHHEEECCCCCEEEEECCCCCCCCEECCCC
>Mature Secondary Structure
MNKKSIRDVEVKGKKVFVRVDFNVPLNENREITDDTRIRAALPTLQYLREQGAKLIVASH
CCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHH
LGRPKGQFNEKYSLKPVAKRLAELLGIEVKMAPDVVGPEVEKMAGELRPGEVLLLENVRF
CCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCCCEEEEECCEE
YPEEEKNDPEFARKLARLAEIFVSDAFGAAHRAHASTAGIASYLPAVAGFLMEKEINFLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALNNPERPFVAIIGGAKVSDKIGVIENLLGKVDVLLIGGGMANTFLKAMGYETGKSLVE
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCHHHHHHHH
EDKVNLAGELMNKAKEVGVKLLLPSDVVVAPRIEAGVPSKIVAVDSIPAEEMALDIGEKT
HHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHH
AKHFREEILKAKTVVWNGPMGVFEVEPFDRGTVAVAEAVAESGALSVVGGGDSVAAVEKA
HHHHHHHHHHHHEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHC
GVADKITHISTGGGASLEFLEGKKLPGVEVLNDK
CCHHHHHEEECCCCCEEEEECCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA