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The map label for this gene is rsmE [H]

Identifier: 78044093

GI number: 78044093

Start: 361829

End: 362575

Strand: Direct

Name: rsmE [H]

Synonym: CHY_0418

Alternate gene names: 78044093

Gene position: 361829-362575 (Clockwise)

Preceding gene: 78044697

Following gene: 78044693

Centisome position: 15.07

GC content: 45.52

Gene sequence:

>747_bases
ATGCCGTACTTTTTTCTACCCCCTGGGCAGACCCAGGGGGATTATGCTTATCTTACCGGTGACGATGCTAAACATGTAGC
CAAGGTTTTAAGAATAAAGCCCGGGGAAAAACTAACATTACAGGACGACCAGGAGTTTCGCTACTTATGCCAGGTAATAG
CGGTAAGAAAAGATTTAGTTCAATTAAAGATTTTGGAGAAAAAAAAGGTTGTAACCAGACCTCCGGTCAATGTAACTTTG
GTGCAGGGGGTACCGAAGGGCGATAAAATGGATTTTATCGTGCAGAAGGCTACGGAACTGGGGGTTTACCGGATAATTCC
GGTGGAAACCATCAGGACGGTAGTGGTTTTTGATGAACGCAAGAAAAAGGATCGATGCGAACGCTGGCAGAAAATTGCTT
ATGAAGCGGCTAAGCAGGCGGGAGTCAGCCGGGTTCCGGAAATTTTTCCGGTAATAGATTTAAAGGCCCTGCCGGAATTT
ATTTCCGATGCTTTAATCTTGGTACCCTACGAAGGCGAGGAGCAAACTTCTTTAAAGCAACTTTTAAATAAAGAGGCGGT
AAAAAACGTTACGGTAATAATAGGCCCCGAAGGGGGGTTTGACCCCGGAGAGATTGAGTATTTAAAAAGTTTTGGGGCGG
TCACTGTTAGCTTGGGGCCAAGAATTTTACGTACGGAAACGGCAGCTCTGGCCGTTCTTTCCATGGTTTTATATCAGTGG
GGTGACCTTGGAGGAGAATTAAGGTGA

Upstream 100 bases:

>100_bases
GAAGTTATGGATAAACTTTTGGAAGTTGGTTTTAAAATTATTGAAAGAAAAAACAGTGGTGGTTGGTGCCTTCTGGTTGC
CAGAAAGGAGTAGGGTGTAA

Downstream 100 bases:

>100_bases
AAAAAGTAGCTTTTTATACGTTAGGTTGTAAAGTAAATCAGTACGAAACCGAAGCGCTAAAAGGAGCTTTTTTAGAGAAA
GGGTATGAAATAGTTGACTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MPYFFLPPGQTQGDYAYLTGDDAKHVAKVLRIKPGEKLTLQDDQEFRYLCQVIAVRKDLVQLKILEKKKVVTRPPVNVTL
VQGVPKGDKMDFIVQKATELGVYRIIPVETIRTVVVFDERKKKDRCERWQKIAYEAAKQAGVSRVPEIFPVIDLKALPEF
ISDALILVPYEGEEQTSLKQLLNKEAVKNVTVIIGPEGGFDPGEIEYLKSFGAVTVSLGPRILRTETAALAVLSMVLYQW
GDLGGELR

Sequences:

>Translated_248_residues
MPYFFLPPGQTQGDYAYLTGDDAKHVAKVLRIKPGEKLTLQDDQEFRYLCQVIAVRKDLVQLKILEKKKVVTRPPVNVTL
VQGVPKGDKMDFIVQKATELGVYRIIPVETIRTVVVFDERKKKDRCERWQKIAYEAAKQAGVSRVPEIFPVIDLKALPEF
ISDALILVPYEGEEQTSLKQLLNKEAVKNVTVIIGPEGGFDPGEIEYLKSFGAVTVSLGPRILRTETAALAVLSMVLYQW
GDLGGELR
>Mature_247_residues
PYFFLPPGQTQGDYAYLTGDDAKHVAKVLRIKPGEKLTLQDDQEFRYLCQVIAVRKDLVQLKILEKKKVVTRPPVNVTLV
QGVPKGDKMDFIVQKATELGVYRIIPVETIRTVVVFDERKKKDRCERWQKIAYEAAKQAGVSRVPEIFPVIDLKALPEFI
SDALILVPYEGEEQTSLKQLLNKEAVKNVTVIIGPEGGFDPGEIEYLKSFGAVTVSLGPRILRTETAALAVLSMVLYQWG
DLGGELR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=254, Percent_Identity=33.4645669291339, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27830; Mature: 27699

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYFFLPPGQTQGDYAYLTGDDAKHVAKVLRIKPGEKLTLQDDQEFRYLCQVIAVRKDLV
CCEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHH
QLKILEKKKVVTRPPVNVTLVQGVPKGDKMDFIVQKATELGVYRIIPVETIRTVVVFDER
HHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEEHHHHEEEEEECCC
KKKDRCERWQKIAYEAAKQAGVSRVPEIFPVIDLKALPEFISDALILVPYEGEEQTSLKQ
CCHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LLNKEAVKNVTVIIGPEGGFDPGEIEYLKSFGAVTVSLGPRILRTETAALAVLSMVLYQW
HHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
GDLGGELR
HCCCCCCC
>Mature Secondary Structure 
PYFFLPPGQTQGDYAYLTGDDAKHVAKVLRIKPGEKLTLQDDQEFRYLCQVIAVRKDLV
CEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHH
QLKILEKKKVVTRPPVNVTLVQGVPKGDKMDFIVQKATELGVYRIIPVETIRTVVVFDER
HHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEEHHHHEEEEEECCC
KKKDRCERWQKIAYEAAKQAGVSRVPEIFPVIDLKALPEFISDALILVPYEGEEQTSLKQ
CCHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LLNKEAVKNVTVIIGPEGGFDPGEIEYLKSFGAVTVSLGPRILRTETAALAVLSMVLYQW
HHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
GDLGGELR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]