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The map label for this gene is yqeV [H]

Identifier: 78044693

GI number: 78044693

Start: 362572

End: 363876

Strand: Direct

Name: yqeV [H]

Synonym: CHY_0419

Alternate gene names: 78044693

Gene position: 362572-363876 (Clockwise)

Preceding gene: 78044093

Following gene: 78043245

Centisome position: 15.1

GC content: 42.68

Gene sequence:

>1305_bases
GTGAAAAAAGTAGCTTTTTATACGTTAGGTTGTAAAGTAAATCAGTACGAAACCGAAGCGCTAAAAGGAGCTTTTTTAGA
GAAAGGGTATGAAATAGTTGACTTTAGCGATTATGCTGATATTTACGTTATAAACACCTGTACTGTTACCCATTTAAGTG
ACCGTAAATCCCGGCAGATGATCAGAAAAGCGGTGCAAAAAAATCCCCGGGCGGTGGTGGCGGCGGTGGGCTGTTATGCT
CAGGTTGCTCCCGAGGAAATTTTAAAAATTCCCGAGGTAAATCTTGTTTTGGGGACCGTTCATAAAAACCGCCTGGTGGA
GTTAGTGGAAAAAGTACTAAGGGAACGGACAAAAATAAATGCGGTGGCCAGTTTTGAAGAGCTCTTGGAATTTGAAGAAA
TGCCTTTAAAGCTCGCACCGGGTAAGGCCCGGGCTTTTGTTAAGATTCAGGAAGGGTGTAATAGCTATTGCGCCTACTGT
ATTATTCCCTATGCCCGGGGACCCCTTAGAAGCCGTCCCTTAGAGGATGTGGTAGCGGAAGTAAAAAAACTCTGTCAGAG
CGGCTTTTCAGAAATTGTGCTTACGGGAATCCATACGGGAGCTTACGGCCAGGAAAAACAGGACTTGCCCAAACTTGCCG
ATTTGGTGGCCGAGTTATTTAAAATTCCCGAACTAAAAAGGCTTCGTTTGAGCTCCATTGAACCGCAGGATTTTACCGTG
GAACTACTGGATGTTCTGGCAAATAGTCCTAAATTCTGCCGTCATCTCCACCTACCCCTGCAAAGTGGTGATGACGATAT
TTTAAAGGCGATGCGCAGGAAATATACTTCTTATGAGTACTTAAGGTTAATTGAAACTATCCGGGAACGGATTCCTGATA
TTGCTTTAACTTCTGATGTTATCGTTGGTTTTCCGGGAGAAACCGAGGAGCAGTTCTTAAACACTTACAATTTAGTAAAA
AAGGTTGGTTTTATGGATATTCACGTTTTTAAATACTCACCCCGGGCGGGTACGCCGGCGGCGAAAATGCCCGGTCAGAT
ACCGGAACGGGAAAAGGAGCGGCGAAGCCTTTTACTTTTAAATTTAAAAGAGGAACTTTTTAAAAATTATGCTTCTAAAT
TTCTCGGTAAAATTTTAGAGGTTATTCCCGAGGAACAGGATACGGAAGGCTTTTGGGAAGGACATAGCGATAATTATTTA
AGAGTCAAGTTTTCGGGCAATAATATAAAGCGGGGAGAAATTTATCCGGTTAAAATTACCGAGATGAAAGAGGGCTATGT
TAGCGGAGAATTGGTAAATAGCTAA

Upstream 100 bases:

>100_bases
CTGTTAGCTTGGGGCCAAGAATTTTACGTACGGAAACGGCAGCTCTGGCCGTTCTTTCCATGGTTTTATATCAGTGGGGT
GACCTTGGAGGAGAATTAAG

Downstream 100 bases:

>100_bases
AATTTTTCTTGCCAGCAGGAATTTTATAGTGTATTGTAGAATTATCTCCTTTTGTAAGGATTGAATTGTACCTTTCTCCC
GAGGGGAGGTGAAGGTATGA

Product: MiaB-like tRNA modifying enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKKVAFYTLGCKVNQYETEALKGAFLEKGYEIVDFSDYADIYVINTCTVTHLSDRKSRQMIRKAVQKNPRAVVAAVGCYA
QVAPEEILKIPEVNLVLGTVHKNRLVELVEKVLRERTKINAVASFEELLEFEEMPLKLAPGKARAFVKIQEGCNSYCAYC
IIPYARGPLRSRPLEDVVAEVKKLCQSGFSEIVLTGIHTGAYGQEKQDLPKLADLVAELFKIPELKRLRLSSIEPQDFTV
ELLDVLANSPKFCRHLHLPLQSGDDDILKAMRRKYTSYEYLRLIETIRERIPDIALTSDVIVGFPGETEEQFLNTYNLVK
KVGFMDIHVFKYSPRAGTPAAKMPGQIPEREKERRSLLLLNLKEELFKNYASKFLGKILEVIPEEQDTEGFWEGHSDNYL
RVKFSGNNIKRGEIYPVKITEMKEGYVSGELVNS

Sequences:

>Translated_434_residues
MKKVAFYTLGCKVNQYETEALKGAFLEKGYEIVDFSDYADIYVINTCTVTHLSDRKSRQMIRKAVQKNPRAVVAAVGCYA
QVAPEEILKIPEVNLVLGTVHKNRLVELVEKVLRERTKINAVASFEELLEFEEMPLKLAPGKARAFVKIQEGCNSYCAYC
IIPYARGPLRSRPLEDVVAEVKKLCQSGFSEIVLTGIHTGAYGQEKQDLPKLADLVAELFKIPELKRLRLSSIEPQDFTV
ELLDVLANSPKFCRHLHLPLQSGDDDILKAMRRKYTSYEYLRLIETIRERIPDIALTSDVIVGFPGETEEQFLNTYNLVK
KVGFMDIHVFKYSPRAGTPAAKMPGQIPEREKERRSLLLLNLKEELFKNYASKFLGKILEVIPEEQDTEGFWEGHSDNYL
RVKFSGNNIKRGEIYPVKITEMKEGYVSGELVNS
>Mature_434_residues
MKKVAFYTLGCKVNQYETEALKGAFLEKGYEIVDFSDYADIYVINTCTVTHLSDRKSRQMIRKAVQKNPRAVVAAVGCYA
QVAPEEILKIPEVNLVLGTVHKNRLVELVEKVLRERTKINAVASFEELLEFEEMPLKLAPGKARAFVKIQEGCNSYCAYC
IIPYARGPLRSRPLEDVVAEVKKLCQSGFSEIVLTGIHTGAYGQEKQDLPKLADLVAELFKIPELKRLRLSSIEPQDFTV
ELLDVLANSPKFCRHLHLPLQSGDDDILKAMRRKYTSYEYLRLIETIRERIPDIALTSDVIVGFPGETEEQFLNTYNLVK
KVGFMDIHVFKYSPRAGTPAAKMPGQIPEREKERRSLLLLNLKEELFKNYASKFLGKILEVIPEEQDTEGFWEGHSDNYL
RVKFSGNNIKRGEIYPVKITEMKEGYVSGELVNS

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=31.1804008908686, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI28872784, Length=437, Percent_Identity=29.9771167048055, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI28872782, Length=386, Percent_Identity=30.8290155440415, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=33.0316742081448, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=29.954954954955, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=28.3870967741935, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71996771, Length=360, Percent_Identity=30, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=29.769392033543, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI19922432, Length=444, Percent_Identity=31.0810810810811, Blast_Score=181, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49382; Mature: 49382

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVAFYTLGCKVNQYETEALKGAFLEKGYEIVDFSDYADIYVINTCTVTHLSDRKSRQM
CCCEEEEEECCEECCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEECCCHHHHHH
IRKAVQKNPRAVVAAVGCYAQVAPEEILKIPEVNLVLGTVHKNRLVELVEKVLRERTKIN
HHHHHHCCCCEEEEHHHHHHHCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
AVASFEELLEFEEMPLKLAPGKARAFVKIQEGCNSYCAYCIIPYARGPLRSRPLEDVVAE
HHHHHHHHHHHHHCCEEECCCCCEEEEEEHHCCCCEEEEEEEECCCCCCCCCCHHHHHHH
VKKLCQSGFSEIVLTGIHTGAYGQEKQDLPKLADLVAELFKIPELKRLRLSSIEPQDFTV
HHHHHHCCHHHEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHH
ELLDVLANSPKFCRHLHLPLQSGDDDILKAMRRKYTSYEYLRLIETIRERIPDIALTSDV
HHHHHHHCCHHHHHEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCE
IVGFPGETEEQFLNTYNLVKKVGFMDIHVFKYSPRAGTPAAKMPGQIPEREKERRSLLLL
EEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCCCHHHHHHHEEEE
NLKEELFKNYASKFLGKILEVIPEEQDTEGFWEGHSDNYLRVKFSGNNIKRGEIYPVKIT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEE
EMKEGYVSGELVNS
CCCCCCCCCEECCC
>Mature Secondary Structure
MKKVAFYTLGCKVNQYETEALKGAFLEKGYEIVDFSDYADIYVINTCTVTHLSDRKSRQM
CCCEEEEEECCEECCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEECCCHHHHHH
IRKAVQKNPRAVVAAVGCYAQVAPEEILKIPEVNLVLGTVHKNRLVELVEKVLRERTKIN
HHHHHHCCCCEEEEHHHHHHHCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
AVASFEELLEFEEMPLKLAPGKARAFVKIQEGCNSYCAYCIIPYARGPLRSRPLEDVVAE
HHHHHHHHHHHHHCCEEECCCCCEEEEEEHHCCCCEEEEEEEECCCCCCCCCCHHHHHHH
VKKLCQSGFSEIVLTGIHTGAYGQEKQDLPKLADLVAELFKIPELKRLRLSSIEPQDFTV
HHHHHHCCHHHEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHH
ELLDVLANSPKFCRHLHLPLQSGDDDILKAMRRKYTSYEYLRLIETIRERIPDIALTSDV
HHHHHHHCCHHHHHEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCE
IVGFPGETEEQFLNTYNLVKKVGFMDIHVFKYSPRAGTPAAKMPGQIPEREKERRSLLLL
EEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCCCHHHHHHHEEEE
NLKEELFKNYASKFLGKILEVIPEEQDTEGFWEGHSDNYLRVKFSGNNIKRGEIYPVKIT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEE
EMKEGYVSGELVNS
CCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]