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The map label for this gene is cobD [H]

Identifier: 78043696

GI number: 78043696

Start: 677175

End: 678134

Strand: Direct

Name: cobD [H]

Synonym: CHY_0770

Alternate gene names: 78043696

Gene position: 677175-678134 (Clockwise)

Preceding gene: 78043382

Following gene: 78043061

Centisome position: 28.2

GC content: 44.06

Gene sequence:

>960_bases
ATGAAAGGAATTTTTATTACCGTGGTTATTGCCTTGATACTGGACATTGTCTTTAAGGATCCAGGGCAAGATTATCATCC
GGTAGCTTTAATTGGTCGTTATATTGCTTTTATAGAAAAAAAGCTTTACCCGAAAAAGCCTGCCAAGAAAGCCTTAATCC
TTTGCGGTGGTTTGCTGGTAGGGCTGACCTTATTGCCGGTAAGTTTCATAGCTTTTATCTACCAGCATTTTACCCAGAAG
CTACCTTTTCTTTTCCAGGTTGTTTTTCAGGGGGGCGGGCTGTTTTTTGCCATAGCCCCTACCGGTTTGGCTTTTTCGGT
TAACACCATAAAAAACCACCTGAAAGATGGTAACCTTGCTGAAGCCCGAAAAAGTCTTGGAATGATTGTTAGCCGTGATA
CCCGGGAATTACCGGTGACGGAAGTGGTAAGGGGAAGTTTGGAGAGCCTTGCCGAAAATACCGGCGATGGGGTAGTGGCT
CCCATATTCTGGTATTTAATTGGAGGACTTCCTGGAGTATGGTTTTACAGGACGGTTAACACTTTAGACAGCATGGTGGG
CTATAAAAGTGAAAGGTATCTTTATTTCGGGCGCATTGCGGCAAAATTGGATGATCTTTTAAATTACCTTCCCGCCCGCA
TCACGGGAGTTTTAATGGTTTTAGCAGCATTTATCTTAAGATATGACTGGAAAAGGGCAATAAAAACCTGGCTTAGAGAT
GCTAAAAAACACCCGAGCCCCAACGGCGGAATTCCCGAAAGCGTCCTGGCGGGAAGTCTTGGGGTCAGGCTTGGAGGCGA
AAACATTTACCACGGGCAGAAAAGCTTTCGGGCTTATTTAGGTGAACCGCTAAATCCGTTAACTCCGGAGGTAATAGATA
AAGCTTTAAAGCTTTTCTGGTTTACAGTATTTTTAATGGTTTTGCTTTTTGGCGGTTTCTCTACGGTAGGAGGGGTATGA

Upstream 100 bases:

>100_bases
TCCTTAAAAAACCTGCGCCAGGAAAATTATGATAAAATAGCCCGGGTGTTCAGGGAAAACGTTAACTTGAAGAAAATTTA
CGAGATTCTGGGGATCTGAG

Downstream 100 bases:

>100_bases
TGAAAGGGTTTATTATAAGTGGTACTACTACAGGAGCTGGCAAAAGTACCTTAACCGTTGGTATCTTAGCAGCTTTAAAA
CACTTGGGCTTAAAGGTGCA

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKGIFITVVIALILDIVFKDPGQDYHPVALIGRYIAFIEKKLYPKKPAKKALILCGGLLVGLTLLPVSFIAFIYQHFTQK
LPFLFQVVFQGGGLFFAIAPTGLAFSVNTIKNHLKDGNLAEARKSLGMIVSRDTRELPVTEVVRGSLESLAENTGDGVVA
PIFWYLIGGLPGVWFYRTVNTLDSMVGYKSERYLYFGRIAAKLDDLLNYLPARITGVLMVLAAFILRYDWKRAIKTWLRD
AKKHPSPNGGIPESVLAGSLGVRLGGENIYHGQKSFRAYLGEPLNPLTPEVIDKALKLFWFTVFLMVLLFGGFSTVGGV

Sequences:

>Translated_319_residues
MKGIFITVVIALILDIVFKDPGQDYHPVALIGRYIAFIEKKLYPKKPAKKALILCGGLLVGLTLLPVSFIAFIYQHFTQK
LPFLFQVVFQGGGLFFAIAPTGLAFSVNTIKNHLKDGNLAEARKSLGMIVSRDTRELPVTEVVRGSLESLAENTGDGVVA
PIFWYLIGGLPGVWFYRTVNTLDSMVGYKSERYLYFGRIAAKLDDLLNYLPARITGVLMVLAAFILRYDWKRAIKTWLRD
AKKHPSPNGGIPESVLAGSLGVRLGGENIYHGQKSFRAYLGEPLNPLTPEVIDKALKLFWFTVFLMVLLFGGFSTVGGV
>Mature_319_residues
MKGIFITVVIALILDIVFKDPGQDYHPVALIGRYIAFIEKKLYPKKPAKKALILCGGLLVGLTLLPVSFIAFIYQHFTQK
LPFLFQVVFQGGGLFFAIAPTGLAFSVNTIKNHLKDGNLAEARKSLGMIVSRDTRELPVTEVVRGSLESLAENTGDGVVA
PIFWYLIGGLPGVWFYRTVNTLDSMVGYKSERYLYFGRIAAKLDDLLNYLPARITGVLMVLAAFILRYDWKRAIKTWLRD
AKKHPSPNGGIPESVLAGSLGVRLGGENIYHGQKSFRAYLGEPLNPLTPEVIDKALKLFWFTVFLMVLLFGGFSTVGGV

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35304; Mature: 35304

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIFITVVIALILDIVFKDPGQDYHPVALIGRYIAFIEKKLYPKKPAKKALILCGGLLV
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GLTLLPVSFIAFIYQHFTQKLPFLFQVVFQGGGLFFAIAPTGLAFSVNTIKNHLKDGNLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCCHH
EARKSLGMIVSRDTRELPVTEVVRGSLESLAENTGDGVVAPIFWYLIGGLPGVWFYRTVN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHH
TLDSMVGYKSERYLYFGRIAAKLDDLLNYLPARITGVLMVLAAFILRYDWKRAIKTWLRD
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKHPSPNGGIPESVLAGSLGVRLGGENIYHGQKSFRAYLGEPLNPLTPEVIDKALKLFW
HHCCCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FTVFLMVLLFGGFSTVGGV
HHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MKGIFITVVIALILDIVFKDPGQDYHPVALIGRYIAFIEKKLYPKKPAKKALILCGGLLV
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GLTLLPVSFIAFIYQHFTQKLPFLFQVVFQGGGLFFAIAPTGLAFSVNTIKNHLKDGNLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCCHH
EARKSLGMIVSRDTRELPVTEVVRGSLESLAENTGDGVVAPIFWYLIGGLPGVWFYRTVN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHH
TLDSMVGYKSERYLYFGRIAAKLDDLLNYLPARITGVLMVLAAFILRYDWKRAIKTWLRD
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKHPSPNGGIPESVLAGSLGVRLGGENIYHGQKSFRAYLGEPLNPLTPEVIDKALKLFW
HHCCCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FTVFLMVLLFGGFSTVGGV
HHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]