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The map label for this gene is ykuI [H]

Identifier: 78043610

GI number: 78043610

Start: 63684

End: 64853

Strand: Direct

Name: ykuI [H]

Synonym: CHY_0074

Alternate gene names: 78043610

Gene position: 63684-64853 (Clockwise)

Preceding gene: 78044978

Following gene: 78045094

Centisome position: 2.65

GC content: 41.03

Gene sequence:

>1170_bases
GTGTCTCCTACCGAAAAAATCGTACCTTTTTTTCAACCGGTGTTCTCGGTGAATACATTCAGCGTATTGGGTTACGAAGT
TTTGGCTAGAAAGATAACGCCCCAGGGTATTGAGAGCCTTGGTGCGTTTTTTCATGACCCCCAGGTGCCACCAGAGAATA
AGATCAAAGTTGACCGTTTTGTTCGCCGCAGGGCATTGGAACTTTTTAAGCAAAGTAACAAAAATTTGCGGCTATTTTTA
AACATCCAGCCCCAGTGGCTTTATCCTTTCATAAGTAAAGAAACAAATTTTCCTACCCTGGAGTACCTTGATGAATACGG
AATTAGCGCTGACCGGGTGATAATTGAAATAAGTGAAACCGAGTTTAGTGCGGACTACGAAAATCTTTCAAGGCTTATTG
ATAGGTACCGTAAAGCTGGGTGTCAGATAGCCGTTGATGATGTTGGTCGTGGTTTCAACAACCTGGAGAGAATTATTGCC
ATACGTCCGGATTTTTTGAAAGTTGATACCCAGTTGGTTAGAAGGAGTATCAAGGAAAATATGGCTCGCAATTTACTGGA
AACACTGGGAAGTTTTGCAGAAAAGTCGGGACTGGGCTTAATACTTGAAGGAATTGAAAGTGCTGAGTTATTTCGCTTGG
GGTTAGAAATCGGAGCCTGTTACTACCAGGGTTTCTTTATTGCCTGTCCGGCACCTCATTTGACAGAAGCTAAAGATTCC
GTTAATTTCATTAAACAAGAAGTCGAAGATTATATCCGCAACGAAATCCATAAACGGAATAGCCAATACCAGAGGAACGA
AGAATTAAATAAACTTATGAAAAATTTTAACTTTTCTAACATTAAACAAAGCCTCGAAGAAAAAATTAGCAATTTAATCC
AATTCCTACCCGACTATTGTTTTCGGGTGTATGCATGCGACTGTTATGGCTATCAAAGGACACCAAACTTTACACGCAAT
TTAGGAGGTGGGTGGACAGTTTCGGGTGAATACCTTAATCGCAACTGGAGCTGGCGTCCGTACTTCTTTCGTGGAGTTAC
TACTGCAAGCCAGCTTGGTAGGGGGATAAGTTCTGAGCCCTACATTGACCTTGAAACTAAGCTAAAAGTATGGACATTTT
GTTATGACCTGGGGGATAATCTTTACTTGTTTATTGACTGTTTAACATGA

Upstream 100 bases:

>100_bases
GCTGCTTTTTAATACAAAAATAAGTTTTTAAAATGAAACTTAACAGGGGTTAAAAATATTTGGGGATAGTTTGAATTAGA
AAGGATGTGAACCTCAAAAG

Downstream 100 bases:

>100_bases
CAGGTTTAATAAAAAATTAAAAGCGGGGGGAATAAACCCCCGCTTTTTTACTCACCGGCTACTTGCTTTTTACCAAATTT
TTGCACCCATAAATCGGCGT

Product: EAL domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSPTEKIVPFFQPVFSVNTFSVLGYEVLARKITPQGIESLGAFFHDPQVPPENKIKVDRFVRRRALELFKQSNKNLRLFL
NIQPQWLYPFISKETNFPTLEYLDEYGISADRVIIEISETEFSADYENLSRLIDRYRKAGCQIAVDDVGRGFNNLERIIA
IRPDFLKVDTQLVRRSIKENMARNLLETLGSFAEKSGLGLILEGIESAELFRLGLEIGACYYQGFFIACPAPHLTEAKDS
VNFIKQEVEDYIRNEIHKRNSQYQRNEELNKLMKNFNFSNIKQSLEEKISNLIQFLPDYCFRVYACDCYGYQRTPNFTRN
LGGGWTVSGEYLNRNWSWRPYFFRGVTTASQLGRGISSEPYIDLETKLKVWTFCYDLGDNLYLFIDCLT

Sequences:

>Translated_389_residues
MSPTEKIVPFFQPVFSVNTFSVLGYEVLARKITPQGIESLGAFFHDPQVPPENKIKVDRFVRRRALELFKQSNKNLRLFL
NIQPQWLYPFISKETNFPTLEYLDEYGISADRVIIEISETEFSADYENLSRLIDRYRKAGCQIAVDDVGRGFNNLERIIA
IRPDFLKVDTQLVRRSIKENMARNLLETLGSFAEKSGLGLILEGIESAELFRLGLEIGACYYQGFFIACPAPHLTEAKDS
VNFIKQEVEDYIRNEIHKRNSQYQRNEELNKLMKNFNFSNIKQSLEEKISNLIQFLPDYCFRVYACDCYGYQRTPNFTRN
LGGGWTVSGEYLNRNWSWRPYFFRGVTTASQLGRGISSEPYIDLETKLKVWTFCYDLGDNLYLFIDCLT
>Mature_388_residues
SPTEKIVPFFQPVFSVNTFSVLGYEVLARKITPQGIESLGAFFHDPQVPPENKIKVDRFVRRRALELFKQSNKNLRLFLN
IQPQWLYPFISKETNFPTLEYLDEYGISADRVIIEISETEFSADYENLSRLIDRYRKAGCQIAVDDVGRGFNNLERIIAI
RPDFLKVDTQLVRRSIKENMARNLLETLGSFAEKSGLGLILEGIESAELFRLGLEIGACYYQGFFIACPAPHLTEAKDSV
NFIKQEVEDYIRNEIHKRNSQYQRNEELNKLMKNFNFSNIKQSLEEKISNLIQFLPDYCFRVYACDCYGYQRTPNFTRNL
GGGWTVSGEYLNRNWSWRPYFFRGVTTASQLGRGISSEPYIDLETKLKVWTFCYDLGDNLYLFIDCLT

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787055, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081921, Length=237, Percent_Identity=25.7383966244726, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788849, Length=182, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790496, Length=233, Percent_Identity=24.8927038626609, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082096, Length=160, Percent_Identity=26.25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786507, Length=236, Percent_Identity=22.8813559322034, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788502, Length=236, Percent_Identity=24.1525423728814, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=26.4705882352941, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633
- InterPro:   IPR018842 [H]

Pfam domain/function: PF00563 EAL; PF10388 YkuI_C [H]

EC number: NA

Molecular weight: Translated: 45230; Mature: 45099

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTEKIVPFFQPVFSVNTFSVLGYEVLARKITPQGIESLGAFFHDPQVPPENKIKVDRF
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
VRRRALELFKQSNKNLRLFLNIQPQWLYPFISKETNFPTLEYLDEYGISADRVIIEISET
HHHHHHHHHHHCCCCEEEEEEECCCEECHHHCCCCCCCHHHHHHHHCCCCCEEEEEEECC
EFSADYENLSRLIDRYRKAGCQIAVDDVGRGFNNLERIIAIRPDFLKVDTQLVRRSIKEN
CCCCCHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHH
MARNLLETLGSFAEKSGLGLILEGIESAELFRLGLEIGACYYQGFFIACPAPHLTEAKDS
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
VNFIKQEVEDYIRNEIHKRNSQYQRNEELNKLMKNFNFSNIKQSLEEKISNLIQFLPDYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FRVYACDCYGYQRTPNFTRNLGGGWTVSGEYLNRNWSWRPYFFRGVTTASQLGRGISSEP
HHHHEEECCCCCCCCCHHHCCCCCEEEECHHHCCCCCCCCCCEECCCCHHHHCCCCCCCC
YIDLETKLKVWTFCYDLGDNLYLFIDCLT
CEEHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SPTEKIVPFFQPVFSVNTFSVLGYEVLARKITPQGIESLGAFFHDPQVPPENKIKVDRF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
VRRRALELFKQSNKNLRLFLNIQPQWLYPFISKETNFPTLEYLDEYGISADRVIIEISET
HHHHHHHHHHHCCCCEEEEEEECCCEECHHHCCCCCCCHHHHHHHHCCCCCEEEEEEECC
EFSADYENLSRLIDRYRKAGCQIAVDDVGRGFNNLERIIAIRPDFLKVDTQLVRRSIKEN
CCCCCHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHH
MARNLLETLGSFAEKSGLGLILEGIESAELFRLGLEIGACYYQGFFIACPAPHLTEAKDS
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
VNFIKQEVEDYIRNEIHKRNSQYQRNEELNKLMKNFNFSNIKQSLEEKISNLIQFLPDYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FRVYACDCYGYQRTPNFTRNLGGGWTVSGEYLNRNWSWRPYFFRGVTTASQLGRGISSEP
HHHHEEECCCCCCCCCHHHCCCCCEEEECHHHCCCCCCCCCCEECCCCHHHHCCCCCCCC
YIDLETKLKVWTFCYDLGDNLYLFIDCLT
CEEHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]