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The map label for this gene is amt [H]

Identifier: 78044978

GI number: 78044978

Start: 62220

End: 63536

Strand: Direct

Name: amt [H]

Synonym: CHY_0073

Alternate gene names: 78044978

Gene position: 62220-63536 (Clockwise)

Preceding gene: 78042932

Following gene: 78043610

Centisome position: 2.59

GC content: 44.87

Gene sequence:

>1317_bases
ATGAAGAAGAAATTTTTATCCTTGCTTTTTGGCCTAATCCTTTTACCTAATTTATCTTTAGCCGGAGAGCCAGGGTATAA
CCCGGGAGATGTTACCTTTGTACTGCTTTCAGCGGCGTTGGTCTTTTTAATGACTCCGGGGCTTGCCCTGTTTTATGGTG
GAATGGTACGCCGGAAAAATGTTTTAAATACGGTGGCGATGAGTTTTATTGCGATAGTGGTAGTTTCACTGCAATGGATT
TTAATAGGCTATACCCTTTCTTTTGGGAATGATGTAAAAGGAATTATCGGAGATTTTTCTTACCTTGGATTAAAAAACGT
AATGGGTCTTACTGTTTCTAACCTTCCCGGATACGTGTTTGTTGTTTTCCAATTAATGTTTGCCATCATAACAGCAGCTA
TTATTTCAGGTTCGGTGGTAGAAAGGATGAGCTTTGCTGCCTGGATCTTATTTATTGCTCTCTGGACGACATTAGTCTAT
GACCCCCTTGCCCATATGGTCTGGGGTGGTGGGATGCTTTTTAAGCTACCGACTTTAGATTTTGCTGGAGGAACGGTGGT
GCATATAAGTTCGGGAATATCGGGCTTGGTGGCAGCACTTATATTAGGTAAAAGAAAAGAAGGGGAAAATGCTCCCTTAA
TGCCGCATAATTTACCGCTTACCATTTTAGGGGCGGGGCTTCTCTGGTTTGGCTGGTTTGGATTTAATGCGGGAAGTGCC
CTTGCAGCCAATAATCTCGCAGCGCTGGCGTTTATTAACACCAATACTTCGGCGGTAATGGCTGCTCTGGCCTGGGCCTT
AGCGGAGTGGCTCCATCGCCGGAAGGTTACGGTTTTAGGTATTGCCACCGGGGCGGTGGCTGGGCTGGTGGCGGTTACTC
CGGCGGCAGGCTTTGTTACCCCAACATCTTCCGTTTTTATTGGCTTAGTCGGTGGGGTCCTGGTCTATATTGCGGTAGGC
ATATTAAAGGCTAAGTTTAATTATGACGATTCTTTGGATGCTTTTGGCTGTCACGGGGTAGGCGGTATCTGGGGAGCAGT
TGCTACGGGCATTTTTGCCACTAAAGCGGTAAACCCGGCGGGAGCTGATGGAGTACTTTACGGAAATTTTGGTCAACTAT
TAAACCAGTTAGCTGGAGTAGGGTTAGCGATTGCATTAGCGGTAGTTGGAACTGTTATAATTTTAAAGTTAATCGGTATT
TTCCTACCTTTGCGGGTTAAAGATGAAGCGGAAAGCATAGGCTTGGATATTACAGTTCATGGGGAAGATGCTTATAAAGA
TTTAATTTTTACCGGGAAGTCGGTTCCGTTTTCTTAA

Upstream 100 bases:

>100_bases
GTGATGGCAAGATTTTTATTAGTGAGGTTTTAGATGCGGTCCGAATTAGAACCGGTGAACGGGGGACAGCAGCTATAACG
TCACCAAAGGAGGGAGAAAG

Downstream 100 bases:

>100_bases
AAAATAAGATAATGGGCAGCTCCTGCGGGTGAAGGTAGGAAGCAGGAGCTGCTTTTTAATACAAAAATAAGTTTTTAAAA
TGAAACTTAACAGGGGTTAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKKKFLSLLFGLILLPNLSLAGEPGYNPGDVTFVLLSAALVFLMTPGLALFYGGMVRRKNVLNTVAMSFIAIVVVSLQWI
LIGYTLSFGNDVKGIIGDFSYLGLKNVMGLTVSNLPGYVFVVFQLMFAIITAAIISGSVVERMSFAAWILFIALWTTLVY
DPLAHMVWGGGMLFKLPTLDFAGGTVVHISSGISGLVAALILGKRKEGENAPLMPHNLPLTILGAGLLWFGWFGFNAGSA
LAANNLAALAFINTNTSAVMAALAWALAEWLHRRKVTVLGIATGAVAGLVAVTPAAGFVTPTSSVFIGLVGGVLVYIAVG
ILKAKFNYDDSLDAFGCHGVGGIWGAVATGIFATKAVNPAGADGVLYGNFGQLLNQLAGVGLAIALAVVGTVIILKLIGI
FLPLRVKDEAESIGLDITVHGEDAYKDLIFTGKSVPFS

Sequences:

>Translated_438_residues
MKKKFLSLLFGLILLPNLSLAGEPGYNPGDVTFVLLSAALVFLMTPGLALFYGGMVRRKNVLNTVAMSFIAIVVVSLQWI
LIGYTLSFGNDVKGIIGDFSYLGLKNVMGLTVSNLPGYVFVVFQLMFAIITAAIISGSVVERMSFAAWILFIALWTTLVY
DPLAHMVWGGGMLFKLPTLDFAGGTVVHISSGISGLVAALILGKRKEGENAPLMPHNLPLTILGAGLLWFGWFGFNAGSA
LAANNLAALAFINTNTSAVMAALAWALAEWLHRRKVTVLGIATGAVAGLVAVTPAAGFVTPTSSVFIGLVGGVLVYIAVG
ILKAKFNYDDSLDAFGCHGVGGIWGAVATGIFATKAVNPAGADGVLYGNFGQLLNQLAGVGLAIALAVVGTVIILKLIGI
FLPLRVKDEAESIGLDITVHGEDAYKDLIFTGKSVPFS
>Mature_438_residues
MKKKFLSLLFGLILLPNLSLAGEPGYNPGDVTFVLLSAALVFLMTPGLALFYGGMVRRKNVLNTVAMSFIAIVVVSLQWI
LIGYTLSFGNDVKGIIGDFSYLGLKNVMGLTVSNLPGYVFVVFQLMFAIITAAIISGSVVERMSFAAWILFIALWTTLVY
DPLAHMVWGGGMLFKLPTLDFAGGTVVHISSGISGLVAALILGKRKEGENAPLMPHNLPLTILGAGLLWFGWFGFNAGSA
LAANNLAALAFINTNTSAVMAALAWALAEWLHRRKVTVLGIATGAVAGLVAVTPAAGFVTPTSSVFIGLVGGVLVYIAVG
ILKAKFNYDDSLDAFGCHGVGGIWGAVATGIFATKAVNPAGADGVLYGNFGQLLNQLAGVGLAIALAVVGTVIILKLIGI
FLPLRVKDEAESIGLDITVHGEDAYKDLIFTGKSVPFS

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=43.5406698564593, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=31.9004524886878, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17550180, Length=440, Percent_Identity=30.4545454545455, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=26.2921348314607, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=415, Percent_Identity=28.433734939759, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=421, Percent_Identity=38.7173396674584, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6325396, Length=415, Percent_Identity=35.9036144578313, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=34.375, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI161078314, Length=411, Percent_Identity=30.1703163017032, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45870; Mature: 45870

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHCCCCCCCCC
>Mature Secondary Structure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CCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]