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The map label for this gene is tilS [H]

Identifier: 78043578

GI number: 78043578

Start: 178622

End: 179971

Strand: Direct

Name: tilS [H]

Synonym: CHY_0213

Alternate gene names: 78043578

Gene position: 178622-179971 (Clockwise)

Preceding gene: 78042785

Following gene: 78043977

Centisome position: 7.44

GC content: 41.33

Gene sequence:

>1350_bases
GTGAACGGGTTGTACGCTAAGTTTGTTAAAACCGTACAAAAATATCAGATGATTAATCCCGGAGAGAAAGTAGTGGTAGG
GGTATCGGGTGGCCCCGACTCCATGGCTCTCCTTTTTTGTTTGCTCAAATACCGGGAAGAAGTGGATTTTTCCCTTGAGG
TTGTTCATGTAAATCACGGTTTAAGGGGAAGGGAAAGCGATTTAGATGAGGAGTTGGTCCTGGATTTTACCCGAAAATAC
GGCATAAATTTTCATGTAAAAAAGCTTGATGATTTAAAAGATTTGCGGGAAAATTTACAGGCGGTAGCCCGGCAAAAACG
GTATGAATTTTTTTATGAAGTTTTGGAAAAAACAGGAGCGGATAAAATAGCGTTAGCTCATACCGCTTCGGATGTAGCGG
AGACGGTGATGATGAATATTTTGCGGGGAGCGGGAACGGAGGGTCTCTGTGGAATTTGGCCGGTGCGGGATAGGATTATT
AGACCATTATATACGGTTTTTCGACGGGAAGTGATGAGTTTTGTTGAACGGAAGAATATACCCTACCGGGTTGATTCTTC
CAACCTTGTGCCCAAGTATTTGCGTAATAAAGTACGTTTAAAGCTTTTTCCGTTTATAAATGAAGAGTTTGCAACCGATA
TCGAAGAGCGGCTTTTTAATCTTGCCCTGATTAAACAGGAAGAAAACCAGGTGCTTGATAATTACTTAAATAAACTGTGG
CAAGAAGCTTTTGTTAAAGAAGGCCAGTACTTAAAGGTACCGGTACTGCAAAACCTTTCCAGGGCGGAAGCTGGACTTTT
GTTGCGCAAGTTTTGGGAACAGGCAACAGGCAGTAAAAGGGATTTATACCAGGAGCACATTGAAAATTTACTGGCCTTTT
GTAACGATATAAAAGGGACCCGCTATTTCCTCTTGCCCCGGGGGATGCGGGCCAAGCTATCCTACGGAAAACTTTTTCTT
GAAAAAAATCTTTGGCAACCAAAGGTTAGCTTTGGATATGAATTGATTCCTCCGGGAAGTTTAGAAATTCCCGAAGCGGG
GAAAAAGATTACCTTAACTCCGGCTTTTAATGGTAAATTTGATTTATTTCTTGCCAAAAGTAGCGGTGTAATCGTCAGAA
ATCGCCGTCCTGGAGATCGATTTACCTTCAGGGGGCATACCAAAAAATTAAAAGAATGGTTGATAGAACAAAAAATACCC
AGGGATATAAGGGATCGTTTACTAATTATTGAAGTAAACGGTCAAATTGCCTGGGTAGAAGGATTGGGGACCGGGGATTT
ATTTAAAAACAGTAAGGATTCTGACCTTATTCCCTTTGCGGTAAACCTTGAATTATTGAAAAGTTGTTGA

Upstream 100 bases:

>100_bases
TTAGCCGAGTATTTCCTTGCCCATGCCAAATTACGCCACCAGAATGTCATTACCGATGACCAGACGGTAATTGTGGTTCG
TTTCTTTGAAAAGACAAGGA

Downstream 100 bases:

>100_bases
AATTATGATAAAATTTTGTAGCATAATGATGGAAAGCTGTGAGAGGAGTGCTCAACTTTGAATAAAGTATTAAAAAATTT
AGCAATTTATTTGTTGATTG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNGLYAKFVKTVQKYQMINPGEKVVVGVSGGPDSMALLFCLLKYREEVDFSLEVVHVNHGLRGRESDLDEELVLDFTRKY
GINFHVKKLDDLKDLRENLQAVARQKRYEFFYEVLEKTGADKIALAHTASDVAETVMMNILRGAGTEGLCGIWPVRDRII
RPLYTVFRREVMSFVERKNIPYRVDSSNLVPKYLRNKVRLKLFPFINEEFATDIEERLFNLALIKQEENQVLDNYLNKLW
QEAFVKEGQYLKVPVLQNLSRAEAGLLLRKFWEQATGSKRDLYQEHIENLLAFCNDIKGTRYFLLPRGMRAKLSYGKLFL
EKNLWQPKVSFGYELIPPGSLEIPEAGKKITLTPAFNGKFDLFLAKSSGVIVRNRRPGDRFTFRGHTKKLKEWLIEQKIP
RDIRDRLLIIEVNGQIAWVEGLGTGDLFKNSKDSDLIPFAVNLELLKSC

Sequences:

>Translated_449_residues
MNGLYAKFVKTVQKYQMINPGEKVVVGVSGGPDSMALLFCLLKYREEVDFSLEVVHVNHGLRGRESDLDEELVLDFTRKY
GINFHVKKLDDLKDLRENLQAVARQKRYEFFYEVLEKTGADKIALAHTASDVAETVMMNILRGAGTEGLCGIWPVRDRII
RPLYTVFRREVMSFVERKNIPYRVDSSNLVPKYLRNKVRLKLFPFINEEFATDIEERLFNLALIKQEENQVLDNYLNKLW
QEAFVKEGQYLKVPVLQNLSRAEAGLLLRKFWEQATGSKRDLYQEHIENLLAFCNDIKGTRYFLLPRGMRAKLSYGKLFL
EKNLWQPKVSFGYELIPPGSLEIPEAGKKITLTPAFNGKFDLFLAKSSGVIVRNRRPGDRFTFRGHTKKLKEWLIEQKIP
RDIRDRLLIIEVNGQIAWVEGLGTGDLFKNSKDSDLIPFAVNLELLKSC
>Mature_449_residues
MNGLYAKFVKTVQKYQMINPGEKVVVGVSGGPDSMALLFCLLKYREEVDFSLEVVHVNHGLRGRESDLDEELVLDFTRKY
GINFHVKKLDDLKDLRENLQAVARQKRYEFFYEVLEKTGADKIALAHTASDVAETVMMNILRGAGTEGLCGIWPVRDRII
RPLYTVFRREVMSFVERKNIPYRVDSSNLVPKYLRNKVRLKLFPFINEEFATDIEERLFNLALIKQEENQVLDNYLNKLW
QEAFVKEGQYLKVPVLQNLSRAEAGLLLRKFWEQATGSKRDLYQEHIENLLAFCNDIKGTRYFLLPRGMRAKLSYGKLFL
EKNLWQPKVSFGYELIPPGSLEIPEAGKKITLTPAFNGKFDLFLAKSSGVIVRNRRPGDRFTFRGHTKKLKEWLIEQKIP
RDIRDRLLIIEVNGQIAWVEGLGTGDLFKNSKDSDLIPFAVNLELLKSC

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=212, Percent_Identity=27.3584905660377, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787606, Length=181, Percent_Identity=22.6519337016575, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51891; Mature: 51891

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLYAKFVKTVQKYQMINPGEKVVVGVSGGPDSMALLFCLLKYREEVDFSLEVVHVNHG
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCC
LRGRESDLDEELVLDFTRKYGINFHVKKLDDLKDLRENLQAVARQKRYEFFYEVLEKTGA
CCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKIALAHTASDVAETVMMNILRGAGTEGLCGIWPVRDRIIRPLYTVFRREVMSFVERKNI
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PYRVDSSNLVPKYLRNKVRLKLFPFINEEFATDIEERLFNLALIKQEENQVLDNYLNKLW
CEEECCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QEAFVKEGQYLKVPVLQNLSRAEAGLLLRKFWEQATGSKRDLYQEHIENLLAFCNDIKGT
HHHHHCCCCEEECHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
RYFLLPRGMRAKLSYGKLFLEKNLWQPKVSFGYELIPPGSLEIPEAGKKITLTPAFNGKF
EEEEECCCCCCHHHHHHHHHHCCCCCCCHHCCEEECCCCCCCCCCCCCEEEEEECCCCCE
DLFLAKSSGVIVRNRRPGDRFTFRGHTKKLKEWLIEQKIPRDIRDRLLIIEVNGQIAWVE
EEEEECCCCEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCHHCCEEEEEECCEEEEEE
GLGTGDLFKNSKDSDLIPFAVNLELLKSC
CCCCCHHHCCCCCCCCCEEEECHHHHHCC
>Mature Secondary Structure
MNGLYAKFVKTVQKYQMINPGEKVVVGVSGGPDSMALLFCLLKYREEVDFSLEVVHVNHG
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCC
LRGRESDLDEELVLDFTRKYGINFHVKKLDDLKDLRENLQAVARQKRYEFFYEVLEKTGA
CCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKIALAHTASDVAETVMMNILRGAGTEGLCGIWPVRDRIIRPLYTVFRREVMSFVERKNI
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PYRVDSSNLVPKYLRNKVRLKLFPFINEEFATDIEERLFNLALIKQEENQVLDNYLNKLW
CEEECCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QEAFVKEGQYLKVPVLQNLSRAEAGLLLRKFWEQATGSKRDLYQEHIENLLAFCNDIKGT
HHHHHCCCCEEECHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
RYFLLPRGMRAKLSYGKLFLEKNLWQPKVSFGYELIPPGSLEIPEAGKKITLTPAFNGKF
EEEEECCCCCCHHHHHHHHHHCCCCCCCHHCCEEECCCCCCCCCCCCCEEEEEECCCCCE
DLFLAKSSGVIVRNRRPGDRFTFRGHTKKLKEWLIEQKIPRDIRDRLLIIEVNGQIAWVE
EEEEECCCCEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCHHCCEEEEEECCEEEEEE
GLGTGDLFKNSKDSDLIPFAVNLELLKSC
CCCCCHHHCCCCCCCCCEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]